Amino acid dipepetide frequency for Bacillus virus 250

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.754AlaAla: 4.754 ± 0.736
0.604AlaCys: 0.604 ± 0.218
3.094AlaAsp: 3.094 ± 0.47
4.905AlaGlu: 4.905 ± 0.614
2.792AlaPhe: 2.792 ± 0.389
3.32AlaGly: 3.32 ± 0.46
1.207AlaHis: 1.207 ± 0.293
5.282AlaIle: 5.282 ± 0.492
5.206AlaLys: 5.206 ± 0.609
6.263AlaLeu: 6.263 ± 1.301
2.792AlaMet: 2.792 ± 0.642
3.094AlaAsn: 3.094 ± 0.485
1.962AlaPro: 1.962 ± 0.369
3.245AlaGln: 3.245 ± 0.526
2.49AlaArg: 2.49 ± 0.454
3.245AlaSer: 3.245 ± 0.531
4.678AlaThr: 4.678 ± 0.754
3.924AlaVal: 3.924 ± 0.619
0.83AlaTrp: 0.83 ± 0.24
2.113AlaTyr: 2.113 ± 0.37
0.0AlaXaa: 0.0 ± 0.0
Cys
0.377CysAla: 0.377 ± 0.151
0.151CysCys: 0.151 ± 0.095
0.981CysAsp: 0.981 ± 0.293
0.981CysGlu: 0.981 ± 0.283
0.377CysPhe: 0.377 ± 0.151
0.604CysGly: 0.604 ± 0.249
0.0CysHis: 0.0 ± 0.0
0.302CysIle: 0.302 ± 0.13
0.528CysLys: 0.528 ± 0.222
0.302CysLeu: 0.302 ± 0.195
0.226CysMet: 0.226 ± 0.141
0.528CysAsn: 0.528 ± 0.228
0.453CysPro: 0.453 ± 0.232
0.0CysGln: 0.0 ± 0.0
0.302CysArg: 0.302 ± 0.168
0.453CysSer: 0.453 ± 0.185
0.528CysThr: 0.528 ± 0.256
0.528CysVal: 0.528 ± 0.213
0.0CysTrp: 0.0 ± 0.0
0.377CysTyr: 0.377 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
4.15AspAla: 4.15 ± 0.631
0.604AspCys: 0.604 ± 0.26
2.565AspAsp: 2.565 ± 0.439
4.452AspGlu: 4.452 ± 0.76
2.716AspPhe: 2.716 ± 0.498
3.018AspGly: 3.018 ± 0.621
0.83AspHis: 0.83 ± 0.267
4.376AspIle: 4.376 ± 0.546
3.924AspLys: 3.924 ± 0.475
4.376AspLeu: 4.376 ± 0.732
1.735AspMet: 1.735 ± 0.423
2.792AspAsn: 2.792 ± 0.371
1.509AspPro: 1.509 ± 0.354
1.962AspGln: 1.962 ± 0.391
2.264AspArg: 2.264 ± 0.44
2.49AspSer: 2.49 ± 0.424
2.49AspThr: 2.49 ± 0.471
4.98AspVal: 4.98 ± 0.668
0.981AspTrp: 0.981 ± 0.258
2.943AspTyr: 2.943 ± 0.526
0.0AspXaa: 0.0 ± 0.0
Glu
4.98GluAla: 4.98 ± 0.615
0.905GluCys: 0.905 ± 0.29
2.792GluAsp: 2.792 ± 0.533
7.093GluGlu: 7.093 ± 1.124
2.867GluPhe: 2.867 ± 0.461
3.622GluGly: 3.622 ± 0.627
1.585GluHis: 1.585 ± 0.392
6.489GluIle: 6.489 ± 0.861
6.489GluLys: 6.489 ± 0.85
7.168GluLeu: 7.168 ± 0.771
2.565GluMet: 2.565 ± 0.557
4.075GluAsn: 4.075 ± 0.589
1.962GluPro: 1.962 ± 0.602
3.245GluGln: 3.245 ± 0.725
3.546GluArg: 3.546 ± 0.598
4.075GluSer: 4.075 ± 0.417
4.452GluThr: 4.452 ± 0.67
5.508GluVal: 5.508 ± 0.661
1.207GluTrp: 1.207 ± 0.313
2.339GluTyr: 2.339 ± 0.458
0.0GluXaa: 0.0 ± 0.0
Phe
2.49PheAla: 2.49 ± 0.422
0.226PheCys: 0.226 ± 0.13
2.943PheAsp: 2.943 ± 0.541
2.641PheGlu: 2.641 ± 0.467
1.358PhePhe: 1.358 ± 0.327
2.264PheGly: 2.264 ± 0.379
0.679PheHis: 0.679 ± 0.239
3.395PheIle: 3.395 ± 0.573
3.169PheLys: 3.169 ± 0.396
2.641PheLeu: 2.641 ± 0.51
0.981PheMet: 0.981 ± 0.252
2.339PheAsn: 2.339 ± 0.465
1.358PhePro: 1.358 ± 0.3
1.358PheGln: 1.358 ± 0.258
2.188PheArg: 2.188 ± 0.404
2.037PheSer: 2.037 ± 0.416
2.339PheThr: 2.339 ± 0.43
2.415PheVal: 2.415 ± 0.471
0.226PheTrp: 0.226 ± 0.131
1.962PheTyr: 1.962 ± 0.292
0.0PheXaa: 0.0 ± 0.0
Gly
3.018GlyAla: 3.018 ± 0.497
0.528GlyCys: 0.528 ± 0.233
3.169GlyAsp: 3.169 ± 0.504
3.848GlyGlu: 3.848 ± 0.514
2.716GlyPhe: 2.716 ± 0.612
3.773GlyGly: 3.773 ± 0.514
0.905GlyHis: 0.905 ± 0.28
5.81GlyIle: 5.81 ± 0.797
5.961GlyLys: 5.961 ± 0.615
3.924GlyLeu: 3.924 ± 0.689
2.867GlyMet: 2.867 ± 0.571
4.301GlyAsn: 4.301 ± 0.67
0.679GlyPro: 0.679 ± 0.245
1.66GlyGln: 1.66 ± 0.325
2.641GlyArg: 2.641 ± 0.433
3.094GlySer: 3.094 ± 0.495
3.546GlyThr: 3.546 ± 0.753
3.773GlyVal: 3.773 ± 0.512
0.679GlyTrp: 0.679 ± 0.222
3.169GlyTyr: 3.169 ± 0.485
0.0GlyXaa: 0.0 ± 0.0
His
0.905HisAla: 0.905 ± 0.264
0.226HisCys: 0.226 ± 0.137
1.056HisAsp: 1.056 ± 0.254
1.283HisGlu: 1.283 ± 0.272
1.132HisPhe: 1.132 ± 0.24
1.056HisGly: 1.056 ± 0.218
0.075HisHis: 0.075 ± 0.069
0.981HisIle: 0.981 ± 0.253
1.358HisLys: 1.358 ± 0.297
2.339HisLeu: 2.339 ± 0.453
0.302HisMet: 0.302 ± 0.143
0.981HisAsn: 0.981 ± 0.292
0.453HisPro: 0.453 ± 0.139
0.83HisGln: 0.83 ± 0.25
0.905HisArg: 0.905 ± 0.255
0.981HisSer: 0.981 ± 0.238
1.283HisThr: 1.283 ± 0.336
0.604HisVal: 0.604 ± 0.27
0.302HisTrp: 0.302 ± 0.12
0.679HisTyr: 0.679 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
5.433IleAla: 5.433 ± 0.588
0.302IleCys: 0.302 ± 0.187
4.376IleAsp: 4.376 ± 0.588
6.112IleGlu: 6.112 ± 0.896
1.735IlePhe: 1.735 ± 0.465
3.395IleGly: 3.395 ± 0.598
1.132IleHis: 1.132 ± 0.294
4.754IleIle: 4.754 ± 0.733
7.319IleLys: 7.319 ± 0.919
4.678IleLeu: 4.678 ± 0.683
1.735IleMet: 1.735 ± 0.368
5.735IleAsn: 5.735 ± 0.81
2.641IlePro: 2.641 ± 0.472
2.867IleGln: 2.867 ± 0.669
2.641IleArg: 2.641 ± 0.428
4.905IleSer: 4.905 ± 0.712
4.452IleThr: 4.452 ± 0.566
4.225IleVal: 4.225 ± 0.672
0.755IleTrp: 0.755 ± 0.24
2.188IleTyr: 2.188 ± 0.448
0.0IleXaa: 0.0 ± 0.0
Lys
5.659LysAla: 5.659 ± 0.531
0.755LysCys: 0.755 ± 0.265
5.81LysAsp: 5.81 ± 0.923
7.998LysGlu: 7.998 ± 1.201
2.339LysPhe: 2.339 ± 0.527
3.773LysGly: 3.773 ± 0.535
1.811LysHis: 1.811 ± 0.396
5.206LysIle: 5.206 ± 0.653
8.828LysLys: 8.828 ± 1.113
6.489LysLeu: 6.489 ± 0.709
2.037LysMet: 2.037 ± 0.412
4.603LysAsn: 4.603 ± 0.521
2.792LysPro: 2.792 ± 0.372
4.678LysGln: 4.678 ± 0.609
5.433LysArg: 5.433 ± 0.692
4.905LysSer: 4.905 ± 0.578
5.357LysThr: 5.357 ± 0.534
4.905LysVal: 4.905 ± 0.675
1.585LysTrp: 1.585 ± 0.359
4.301LysTyr: 4.301 ± 0.717
0.0LysXaa: 0.0 ± 0.0
Leu
5.433LeuAla: 5.433 ± 0.906
0.528LeuCys: 0.528 ± 0.205
4.905LeuAsp: 4.905 ± 0.501
6.414LeuGlu: 6.414 ± 0.762
3.018LeuPhe: 3.018 ± 0.518
4.905LeuGly: 4.905 ± 0.689
0.981LeuHis: 0.981 ± 0.295
4.829LeuIle: 4.829 ± 0.683
7.998LeuLys: 7.998 ± 0.825
6.64LeuLeu: 6.64 ± 0.809
2.565LeuMet: 2.565 ± 0.392
5.131LeuAsn: 5.131 ± 0.696
2.867LeuPro: 2.867 ± 0.826
2.943LeuGln: 2.943 ± 0.433
3.697LeuArg: 3.697 ± 0.513
4.754LeuSer: 4.754 ± 0.602
4.829LeuThr: 4.829 ± 0.795
3.999LeuVal: 3.999 ± 0.658
0.528LeuTrp: 0.528 ± 0.176
2.943LeuTyr: 2.943 ± 0.46
0.0LeuXaa: 0.0 ± 0.0
Met
2.716MetAla: 2.716 ± 0.621
0.226MetCys: 0.226 ± 0.141
1.434MetAsp: 1.434 ± 0.281
1.886MetGlu: 1.886 ± 0.314
0.83MetPhe: 0.83 ± 0.217
1.434MetGly: 1.434 ± 0.491
0.83MetHis: 0.83 ± 0.277
1.811MetIle: 1.811 ± 0.394
3.773MetLys: 3.773 ± 0.477
2.641MetLeu: 2.641 ± 0.57
1.283MetMet: 1.283 ± 0.353
2.188MetAsn: 2.188 ± 0.419
0.905MetPro: 0.905 ± 0.287
1.132MetGln: 1.132 ± 0.223
1.585MetArg: 1.585 ± 0.435
2.867MetSer: 2.867 ± 0.408
1.811MetThr: 1.811 ± 0.347
0.755MetVal: 0.755 ± 0.243
0.075MetTrp: 0.075 ± 0.073
1.056MetTyr: 1.056 ± 0.234
0.0MetXaa: 0.0 ± 0.0
Asn
3.32AsnAla: 3.32 ± 0.661
0.679AsnCys: 0.679 ± 0.242
3.773AsnAsp: 3.773 ± 0.561
4.301AsnGlu: 4.301 ± 0.533
1.886AsnPhe: 1.886 ± 0.287
5.357AsnGly: 5.357 ± 0.582
1.811AsnHis: 1.811 ± 0.43
3.924AsnIle: 3.924 ± 0.625
5.584AsnLys: 5.584 ± 0.707
4.301AsnLeu: 4.301 ± 0.573
2.188AsnMet: 2.188 ± 0.325
3.32AsnAsn: 3.32 ± 0.514
2.867AsnPro: 2.867 ± 0.316
2.867AsnGln: 2.867 ± 0.377
1.962AsnArg: 1.962 ± 0.396
3.245AsnSer: 3.245 ± 0.76
2.943AsnThr: 2.943 ± 0.575
3.018AsnVal: 3.018 ± 0.451
0.377AsnTrp: 0.377 ± 0.157
1.886AsnTyr: 1.886 ± 0.454
0.0AsnXaa: 0.0 ± 0.0
Pro
1.66ProAla: 1.66 ± 0.297
0.226ProCys: 0.226 ± 0.135
1.585ProAsp: 1.585 ± 0.404
1.735ProGlu: 1.735 ± 0.41
1.132ProPhe: 1.132 ± 0.307
1.358ProGly: 1.358 ± 0.386
0.528ProHis: 0.528 ± 0.211
2.188ProIle: 2.188 ± 0.325
2.113ProLys: 2.113 ± 0.477
2.037ProLeu: 2.037 ± 0.385
0.981ProMet: 0.981 ± 0.22
2.188ProAsn: 2.188 ± 0.39
1.358ProPro: 1.358 ± 0.495
1.735ProGln: 1.735 ± 0.316
1.358ProArg: 1.358 ± 0.37
2.641ProSer: 2.641 ± 0.329
2.339ProThr: 2.339 ± 0.414
3.094ProVal: 3.094 ± 0.6
0.226ProTrp: 0.226 ± 0.129
1.509ProTyr: 1.509 ± 0.28
0.0ProXaa: 0.0 ± 0.0
Gln
2.867GlnAla: 2.867 ± 0.571
0.075GlnCys: 0.075 ± 0.072
1.735GlnAsp: 1.735 ± 0.325
2.943GlnGlu: 2.943 ± 0.701
1.056GlnPhe: 1.056 ± 0.361
2.264GlnGly: 2.264 ± 0.418
0.604GlnHis: 0.604 ± 0.198
3.245GlnIle: 3.245 ± 0.571
2.716GlnLys: 2.716 ± 0.44
4.301GlnLeu: 4.301 ± 0.672
1.434GlnMet: 1.434 ± 0.332
1.735GlnAsn: 1.735 ± 0.343
1.207GlnPro: 1.207 ± 0.24
2.037GlnGln: 2.037 ± 0.576
2.943GlnArg: 2.943 ± 0.496
1.962GlnSer: 1.962 ± 0.33
3.018GlnThr: 3.018 ± 0.383
1.811GlnVal: 1.811 ± 0.353
0.679GlnTrp: 0.679 ± 0.22
1.509GlnTyr: 1.509 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
2.037ArgAla: 2.037 ± 0.447
0.377ArgCys: 0.377 ± 0.159
2.641ArgAsp: 2.641 ± 0.445
3.245ArgGlu: 3.245 ± 0.551
1.962ArgPhe: 1.962 ± 0.317
2.867ArgGly: 2.867 ± 0.513
0.981ArgHis: 0.981 ± 0.298
3.622ArgIle: 3.622 ± 0.566
5.055ArgLys: 5.055 ± 0.788
3.622ArgLeu: 3.622 ± 0.505
1.735ArgMet: 1.735 ± 0.394
2.264ArgAsn: 2.264 ± 0.345
1.358ArgPro: 1.358 ± 0.389
1.735ArgGln: 1.735 ± 0.434
2.792ArgArg: 2.792 ± 0.415
1.735ArgSer: 1.735 ± 0.331
2.188ArgThr: 2.188 ± 0.521
3.169ArgVal: 3.169 ± 0.449
0.679ArgTrp: 0.679 ± 0.266
2.037ArgTyr: 2.037 ± 0.403
0.0ArgXaa: 0.0 ± 0.0
Ser
3.169SerAla: 3.169 ± 0.602
0.302SerCys: 0.302 ± 0.126
3.094SerAsp: 3.094 ± 0.346
3.924SerGlu: 3.924 ± 0.617
3.094SerPhe: 3.094 ± 0.424
4.15SerGly: 4.15 ± 0.488
0.905SerHis: 0.905 ± 0.227
3.697SerIle: 3.697 ± 0.523
4.905SerLys: 4.905 ± 0.624
5.433SerLeu: 5.433 ± 0.702
1.434SerMet: 1.434 ± 0.359
3.697SerAsn: 3.697 ± 0.687
1.585SerPro: 1.585 ± 0.341
2.113SerGln: 2.113 ± 0.395
2.264SerArg: 2.264 ± 0.438
3.471SerSer: 3.471 ± 0.688
2.792SerThr: 2.792 ± 0.533
3.546SerVal: 3.546 ± 0.72
0.755SerTrp: 0.755 ± 0.211
2.037SerTyr: 2.037 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
5.433ThrAla: 5.433 ± 0.786
0.302ThrCys: 0.302 ± 0.19
2.264ThrAsp: 2.264 ± 0.394
3.546ThrGlu: 3.546 ± 0.456
3.471ThrPhe: 3.471 ± 0.625
5.055ThrGly: 5.055 ± 0.658
1.132ThrHis: 1.132 ± 0.231
4.301ThrIle: 4.301 ± 0.767
4.829ThrLys: 4.829 ± 0.614
5.357ThrLeu: 5.357 ± 0.747
1.132ThrMet: 1.132 ± 0.376
4.225ThrAsn: 4.225 ± 0.51
3.169ThrPro: 3.169 ± 0.666
1.585ThrGln: 1.585 ± 0.427
1.811ThrArg: 1.811 ± 0.491
3.395ThrSer: 3.395 ± 0.486
3.848ThrThr: 3.848 ± 0.46
3.471ThrVal: 3.471 ± 0.488
0.377ThrTrp: 0.377 ± 0.17
1.585ThrTyr: 1.585 ± 0.302
0.0ThrXaa: 0.0 ± 0.0
Val
3.848ValAla: 3.848 ± 0.538
0.377ValCys: 0.377 ± 0.176
4.225ValAsp: 4.225 ± 0.69
4.905ValGlu: 4.905 ± 0.667
2.641ValPhe: 2.641 ± 0.499
4.603ValGly: 4.603 ± 0.717
0.83ValHis: 0.83 ± 0.289
3.622ValIle: 3.622 ± 0.501
5.357ValLys: 5.357 ± 0.723
3.848ValLeu: 3.848 ± 0.515
1.811ValMet: 1.811 ± 0.487
3.245ValAsn: 3.245 ± 0.508
2.113ValPro: 2.113 ± 0.433
2.113ValGln: 2.113 ± 0.415
2.641ValArg: 2.641 ± 0.525
3.169ValSer: 3.169 ± 0.532
4.376ValThr: 4.376 ± 0.81
3.848ValVal: 3.848 ± 0.54
0.679ValTrp: 0.679 ± 0.221
1.811ValTyr: 1.811 ± 0.392
0.0ValXaa: 0.0 ± 0.0
Trp
0.905TrpAla: 0.905 ± 0.28
0.151TrpCys: 0.151 ± 0.1
0.905TrpAsp: 0.905 ± 0.278
1.207TrpGlu: 1.207 ± 0.359
0.528TrpPhe: 0.528 ± 0.204
0.453TrpGly: 0.453 ± 0.172
0.377TrpHis: 0.377 ± 0.227
0.755TrpIle: 0.755 ± 0.217
0.83TrpLys: 0.83 ± 0.244
0.604TrpLeu: 0.604 ± 0.203
0.377TrpMet: 0.377 ± 0.185
0.905TrpAsn: 0.905 ± 0.245
0.0TrpPro: 0.0 ± 0.0
0.226TrpGln: 0.226 ± 0.142
0.453TrpArg: 0.453 ± 0.16
0.83TrpSer: 0.83 ± 0.293
0.905TrpThr: 0.905 ± 0.276
0.528TrpVal: 0.528 ± 0.149
0.151TrpTrp: 0.151 ± 0.104
0.377TrpTyr: 0.377 ± 0.199
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.641TyrAla: 2.641 ± 0.443
0.453TyrCys: 0.453 ± 0.197
1.811TyrAsp: 1.811 ± 0.37
3.546TyrGlu: 3.546 ± 0.719
1.358TyrPhe: 1.358 ± 0.308
2.792TyrGly: 2.792 ± 0.408
0.528TyrHis: 0.528 ± 0.236
2.792TyrIle: 2.792 ± 0.433
3.32TyrLys: 3.32 ± 0.486
2.943TyrLeu: 2.943 ± 0.389
1.056TyrMet: 1.056 ± 0.243
2.264TyrAsn: 2.264 ± 0.385
0.755TyrPro: 0.755 ± 0.255
1.811TyrGln: 1.811 ± 0.419
2.188TyrArg: 2.188 ± 0.4
2.113TyrSer: 2.113 ± 0.473
2.113TyrThr: 2.113 ± 0.39
1.962TyrVal: 1.962 ± 0.337
0.377TyrTrp: 0.377 ± 0.219
2.641TyrTyr: 2.641 ± 0.499
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13254 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski