Ahrensia sp. R2A130

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Ahrensiaceae; Ahrensia; unclassified Ahrensia

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3622 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0MJH6|E0MJH6_9RHOB Uncharacterized protein OS=Ahrensia sp. R2A130 OX=744979 GN=R2A130_0040 PE=4 SV=1
MM1 pKa = 7.48HH2 pKa = 7.89ALKK5 pKa = 10.46QEE7 pKa = 4.18QFCTDD12 pKa = 3.23ITPAQAFLASDD23 pKa = 4.23PASVACDD30 pKa = 3.44VMDD33 pKa = 3.38MWTEE37 pKa = 4.11DD38 pKa = 2.93GTLIVISADD47 pKa = 3.34ALPSATLQGLQNCWLNGDD65 pKa = 3.77VEE67 pKa = 4.96AYY69 pKa = 10.35RR70 pKa = 11.84AMDD73 pKa = 3.22MMGG76 pKa = 2.99

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0MR67|E0MR67_9RHOB Uncharacterized protein OS=Ahrensia sp. R2A130 OX=744979 GN=R2A130_2910 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.26VKK4 pKa = 10.85NSLRR8 pKa = 11.84SLKK11 pKa = 10.09GRR13 pKa = 11.84HH14 pKa = 4.78RR15 pKa = 11.84ANRR18 pKa = 11.84LVRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 10.11GRR25 pKa = 11.84VYY27 pKa = 10.41IINKK31 pKa = 7.68VNPRR35 pKa = 11.84FKK37 pKa = 10.77ARR39 pKa = 11.84QGG41 pKa = 3.28

Molecular weight: 4.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3622

0

3622

1133679

37

7333

313.0

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.64 ± 0.067

0.86 ± 0.015

6.398 ± 0.077

5.807 ± 0.046

3.886 ± 0.028

8.515 ± 0.064

1.981 ± 0.028

5.461 ± 0.035

3.797 ± 0.048

9.363 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.033

3.152 ± 0.036

4.706 ± 0.032

3.134 ± 0.028

6.034 ± 0.061

5.775 ± 0.032

5.92 ± 0.065

7.339 ± 0.04

1.298 ± 0.019

2.164 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski