Lactococcus phage WP-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Negarvirus; Lactococcus virus WP2

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024B482|A0A024B482_9CAUD Lower collar protein OS=Lactococcus phage WP-2 OX=1486423 GN=WP2_21 PE=4 SV=1
MM1 pKa = 7.38TVPLDD6 pKa = 3.53NDD8 pKa = 3.95GQSVGTALNTIDD20 pKa = 5.5SIANYY25 pKa = 8.62MRR27 pKa = 11.84HH28 pKa = 5.64CRR30 pKa = 11.84YY31 pKa = 8.13NTTDD35 pKa = 2.53NWLFDD40 pKa = 3.86SFINSYY46 pKa = 11.37ANEE49 pKa = 3.91QILINICMQPFYY61 pKa = 11.28GIITLNNYY69 pKa = 10.05CNFDD73 pKa = 3.79YY74 pKa = 10.68FHH76 pKa = 6.96NWLINNDD83 pKa = 4.23EE84 pKa = 4.11ILEE87 pKa = 4.33DD88 pKa = 4.04KK89 pKa = 10.32PLSEE93 pKa = 3.47IDD95 pKa = 3.59YY96 pKa = 8.57YY97 pKa = 10.98QEE99 pKa = 3.58YY100 pKa = 10.96DD101 pKa = 4.1LIYY104 pKa = 10.73LLSEE108 pKa = 4.25SLNPTVGMIWKK119 pKa = 8.04YY120 pKa = 7.3TTDD123 pKa = 2.86TDD125 pKa = 3.84YY126 pKa = 11.21RR127 pKa = 11.84YY128 pKa = 8.99LTFDD132 pKa = 3.62PVNNFYY138 pKa = 11.0EE139 pKa = 4.74GTLNDD144 pKa = 4.26YY145 pKa = 11.0IKK147 pKa = 10.15MYY149 pKa = 10.49PEE151 pKa = 3.65YY152 pKa = 10.66TNYY155 pKa = 10.45QLVRR159 pKa = 11.84PTAFISDD166 pKa = 3.94TQQ168 pKa = 3.33

Molecular weight: 19.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024B467|A0A024B467_9CAUD Peptidase C51 domain-containing protein OS=Lactococcus phage WP-2 OX=1486423 GN=WP2_01 PE=4 SV=1
MM1 pKa = 7.27GTTNQQITEE10 pKa = 4.08MPVRR14 pKa = 11.84HH15 pKa = 6.01FPDD18 pKa = 2.78SQRR21 pKa = 11.84FDD23 pKa = 3.36NGQGMSRR30 pKa = 11.84LDD32 pKa = 3.09MSKK35 pKa = 10.17QYY37 pKa = 10.79KK38 pKa = 8.6STMSIQPSAGDD49 pKa = 3.41SYY51 pKa = 11.59TRR53 pKa = 11.84AIHH56 pKa = 5.98NPRR59 pKa = 11.84GG60 pKa = 3.44

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

5765

60

799

240.2

27.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.082 ± 0.438

0.468 ± 0.082

6.47 ± 0.3

6.123 ± 0.615

4.944 ± 0.521

5.776 ± 0.792

1.613 ± 0.213

7.129 ± 0.239

7.719 ± 0.654

8.049 ± 0.481

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.264

8.326 ± 0.497

3.348 ± 0.396

4.354 ± 0.456

2.984 ± 0.233

5.846 ± 0.3

7.476 ± 0.5

5.377 ± 0.293

1.023 ± 0.159

5.169 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski