Amino acid dipepetide frequency for Lactococcus phage WP-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.347AlaAla: 0.347 ± 0.231
0.0AlaCys: 0.0 ± 0.0
2.776AlaAsp: 2.776 ± 0.525
3.123AlaGlu: 3.123 ± 0.734
3.123AlaPhe: 3.123 ± 0.848
3.817AlaGly: 3.817 ± 1.106
0.867AlaHis: 0.867 ± 0.441
4.684AlaIle: 4.684 ± 0.797
3.296AlaLys: 3.296 ± 0.726
5.725AlaLeu: 5.725 ± 0.998
1.214AlaMet: 1.214 ± 0.517
4.684AlaAsn: 4.684 ± 1.146
1.388AlaPro: 1.388 ± 0.598
3.296AlaGln: 3.296 ± 0.797
1.388AlaArg: 1.388 ± 0.45
3.123AlaSer: 3.123 ± 0.607
3.296AlaThr: 3.296 ± 0.636
2.429AlaVal: 2.429 ± 0.534
0.867AlaTrp: 0.867 ± 0.39
1.388AlaTyr: 1.388 ± 0.528
0.0AlaXaa: 0.0 ± 0.0
Cys
0.173CysAla: 0.173 ± 0.171
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.173CysGlu: 0.173 ± 0.171
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
0.867CysIle: 0.867 ± 0.398
0.0CysLys: 0.0 ± 0.0
0.347CysLeu: 0.347 ± 0.216
0.347CysMet: 0.347 ± 0.242
0.694CysAsn: 0.694 ± 0.287
0.173CysPro: 0.173 ± 0.161
0.52CysGln: 0.52 ± 0.248
0.52CysArg: 0.52 ± 0.263
0.173CysSer: 0.173 ± 0.196
0.0CysThr: 0.0 ± 0.0
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.694CysTyr: 0.694 ± 0.267
0.0CysXaa: 0.0 ± 0.0
Asp
0.694AspAla: 0.694 ± 0.383
0.347AspCys: 0.347 ± 0.226
4.337AspAsp: 4.337 ± 0.793
4.337AspGlu: 4.337 ± 0.864
2.949AspPhe: 2.949 ± 0.808
4.684AspGly: 4.684 ± 1.15
0.867AspHis: 0.867 ± 0.383
5.378AspIle: 5.378 ± 1.13
5.378AspLys: 5.378 ± 0.739
6.593AspLeu: 6.593 ± 0.931
2.082AspMet: 2.082 ± 0.567
5.899AspAsn: 5.899 ± 1.013
1.735AspPro: 1.735 ± 0.401
1.561AspGln: 1.561 ± 0.581
1.388AspArg: 1.388 ± 0.67
3.296AspSer: 3.296 ± 0.697
3.296AspThr: 3.296 ± 0.713
4.684AspVal: 4.684 ± 0.939
0.52AspTrp: 0.52 ± 0.299
4.684AspTyr: 4.684 ± 0.858
0.0AspXaa: 0.0 ± 0.0
Glu
2.776GluAla: 2.776 ± 0.817
0.52GluCys: 0.52 ± 0.26
3.817GluAsp: 3.817 ± 0.778
5.378GluGlu: 5.378 ± 1.183
2.255GluPhe: 2.255 ± 0.585
3.296GluGly: 3.296 ± 0.632
0.694GluHis: 0.694 ± 0.314
4.164GluIle: 4.164 ± 1.147
5.031GluLys: 5.031 ± 1.161
5.031GluLeu: 5.031 ± 0.828
2.255GluMet: 2.255 ± 0.572
5.031GluAsn: 5.031 ± 1.138
1.388GluPro: 1.388 ± 0.382
3.123GluGln: 3.123 ± 0.792
3.296GluArg: 3.296 ± 0.804
1.388GluSer: 1.388 ± 0.406
3.643GluThr: 3.643 ± 0.896
3.99GluVal: 3.99 ± 0.965
0.694GluTrp: 0.694 ± 0.351
3.47GluTyr: 3.47 ± 0.864
0.0GluXaa: 0.0 ± 0.0
Phe
2.255PheAla: 2.255 ± 0.853
0.347PheCys: 0.347 ± 0.204
3.296PheAsp: 3.296 ± 0.932
2.082PheGlu: 2.082 ± 0.781
1.735PhePhe: 1.735 ± 0.792
3.123PheGly: 3.123 ± 0.609
1.041PheHis: 1.041 ± 0.544
3.99PheIle: 3.99 ± 0.699
3.643PheLys: 3.643 ± 0.933
2.255PheLeu: 2.255 ± 0.532
1.561PheMet: 1.561 ± 0.673
4.337PheAsn: 4.337 ± 1.099
2.082PhePro: 2.082 ± 0.642
1.908PheGln: 1.908 ± 0.637
1.388PheArg: 1.388 ± 0.448
3.643PheSer: 3.643 ± 0.751
3.817PheThr: 3.817 ± 0.907
2.602PheVal: 2.602 ± 0.55
0.173PheTrp: 0.173 ± 0.164
4.164PheTyr: 4.164 ± 1.047
0.0PheXaa: 0.0 ± 0.0
Gly
3.643GlyAla: 3.643 ± 0.886
0.173GlyCys: 0.173 ± 0.164
3.123GlyAsp: 3.123 ± 0.911
2.602GlyGlu: 2.602 ± 0.648
3.123GlyPhe: 3.123 ± 0.789
4.684GlyGly: 4.684 ± 1.205
0.867GlyHis: 0.867 ± 0.406
4.858GlyIle: 4.858 ± 0.956
4.164GlyLys: 4.164 ± 0.955
3.47GlyLeu: 3.47 ± 0.713
1.908GlyMet: 1.908 ± 0.679
4.684GlyAsn: 4.684 ± 1.279
0.173GlyPro: 0.173 ± 0.171
2.949GlyGln: 2.949 ± 0.915
1.735GlyArg: 1.735 ± 0.591
3.817GlySer: 3.817 ± 1.37
5.378GlyThr: 5.378 ± 1.228
2.255GlyVal: 2.255 ± 0.619
0.867GlyTrp: 0.867 ± 0.32
3.296GlyTyr: 3.296 ± 0.866
0.0GlyXaa: 0.0 ± 0.0
His
1.041HisAla: 1.041 ± 0.364
0.173HisCys: 0.173 ± 0.164
1.214HisAsp: 1.214 ± 0.509
0.347HisGlu: 0.347 ± 0.322
1.908HisPhe: 1.908 ± 0.449
0.52HisGly: 0.52 ± 0.348
0.173HisHis: 0.173 ± 0.183
0.694HisIle: 0.694 ± 0.312
1.214HisLys: 1.214 ± 0.572
1.388HisLeu: 1.388 ± 0.496
0.347HisMet: 0.347 ± 0.228
1.388HisAsn: 1.388 ± 0.55
0.173HisPro: 0.173 ± 0.171
0.694HisGln: 0.694 ± 0.251
0.694HisArg: 0.694 ± 0.345
0.694HisSer: 0.694 ± 0.347
1.735HisThr: 1.735 ± 0.426
0.867HisVal: 0.867 ± 0.532
0.0HisTrp: 0.0 ± 0.0
0.867HisTyr: 0.867 ± 0.368
0.0HisXaa: 0.0 ± 0.0
Ile
4.164IleAla: 4.164 ± 0.616
0.347IleCys: 0.347 ± 0.242
5.899IleAsp: 5.899 ± 1.209
7.113IleGlu: 7.113 ± 1.494
1.908IlePhe: 1.908 ± 0.681
4.684IleGly: 4.684 ± 1.448
1.214IleHis: 1.214 ± 0.451
2.776IleIle: 2.776 ± 0.624
7.807IleLys: 7.807 ± 0.871
5.725IleLeu: 5.725 ± 1.168
2.429IleMet: 2.429 ± 0.868
4.164IleAsn: 4.164 ± 0.896
1.908IlePro: 1.908 ± 0.483
2.255IleGln: 2.255 ± 0.618
1.908IleArg: 1.908 ± 0.558
2.949IleSer: 2.949 ± 0.573
5.899IleThr: 5.899 ± 0.604
3.123IleVal: 3.123 ± 0.906
1.388IleTrp: 1.388 ± 0.288
3.643IleTyr: 3.643 ± 0.737
0.0IleXaa: 0.0 ± 0.0
Lys
2.776LysAla: 2.776 ± 0.629
0.173LysCys: 0.173 ± 0.161
6.419LysAsp: 6.419 ± 1.051
4.164LysGlu: 4.164 ± 1.279
4.164LysPhe: 4.164 ± 0.984
4.164LysGly: 4.164 ± 1.21
1.214LysHis: 1.214 ± 0.343
5.205LysIle: 5.205 ± 1.094
4.511LysLys: 4.511 ± 1.341
6.072LysLeu: 6.072 ± 1.302
2.255LysMet: 2.255 ± 0.608
6.593LysAsn: 6.593 ± 0.944
4.164LysPro: 4.164 ± 1.701
3.123LysGln: 3.123 ± 0.649
2.429LysArg: 2.429 ± 0.792
4.858LysSer: 4.858 ± 0.895
5.552LysThr: 5.552 ± 0.736
5.031LysVal: 5.031 ± 1.034
0.347LysTrp: 0.347 ± 0.216
3.99LysTyr: 3.99 ± 0.952
0.0LysXaa: 0.0 ± 0.0
Leu
5.031LeuAla: 5.031 ± 1.084
0.173LeuCys: 0.173 ± 0.171
4.164LeuAsp: 4.164 ± 1.157
5.205LeuGlu: 5.205 ± 1.087
2.429LeuPhe: 2.429 ± 0.633
5.205LeuGly: 5.205 ± 1.287
2.949LeuHis: 2.949 ± 0.712
5.725LeuIle: 5.725 ± 0.799
7.634LeuLys: 7.634 ± 1.213
6.246LeuLeu: 6.246 ± 1.432
2.255LeuMet: 2.255 ± 0.562
6.766LeuAsn: 6.766 ± 1.178
2.602LeuPro: 2.602 ± 0.615
2.255LeuGln: 2.255 ± 0.702
2.602LeuArg: 2.602 ± 0.549
6.072LeuSer: 6.072 ± 0.694
5.552LeuThr: 5.552 ± 1.115
3.123LeuVal: 3.123 ± 0.862
1.041LeuTrp: 1.041 ± 0.425
3.47LeuTyr: 3.47 ± 0.737
0.0LeuXaa: 0.0 ± 0.0
Met
1.561MetAla: 1.561 ± 0.636
0.0MetCys: 0.0 ± 0.0
1.388MetAsp: 1.388 ± 0.515
1.561MetGlu: 1.561 ± 0.512
1.908MetPhe: 1.908 ± 0.601
1.041MetGly: 1.041 ± 0.452
0.52MetHis: 0.52 ± 0.269
2.949MetIle: 2.949 ± 0.602
3.47MetLys: 3.47 ± 0.689
1.388MetLeu: 1.388 ± 0.615
0.347MetMet: 0.347 ± 0.232
2.429MetAsn: 2.429 ± 0.534
0.867MetPro: 0.867 ± 0.35
0.867MetGln: 0.867 ± 0.384
0.694MetArg: 0.694 ± 0.3
2.082MetSer: 2.082 ± 0.902
1.388MetThr: 1.388 ± 0.622
1.388MetVal: 1.388 ± 0.588
0.173MetTrp: 0.173 ± 0.161
1.214MetTyr: 1.214 ± 0.326
0.0MetXaa: 0.0 ± 0.0
Asn
3.99AsnAla: 3.99 ± 1.004
0.173AsnCys: 0.173 ± 0.188
5.205AsnAsp: 5.205 ± 0.658
3.123AsnGlu: 3.123 ± 0.743
4.511AsnPhe: 4.511 ± 0.759
5.205AsnGly: 5.205 ± 1.075
1.214AsnHis: 1.214 ± 0.614
4.337AsnIle: 4.337 ± 1.111
4.511AsnLys: 4.511 ± 1.2
6.072AsnLeu: 6.072 ± 1.144
1.908AsnMet: 1.908 ± 0.556
6.94AsnAsn: 6.94 ± 1.422
5.205AsnPro: 5.205 ± 1.17
4.684AsnGln: 4.684 ± 0.861
2.255AsnArg: 2.255 ± 0.421
5.378AsnSer: 5.378 ± 1.303
7.981AsnThr: 7.981 ± 2.008
4.858AsnVal: 4.858 ± 1.381
1.735AsnTrp: 1.735 ± 0.593
3.99AsnTyr: 3.99 ± 0.734
0.0AsnXaa: 0.0 ± 0.0
Pro
1.388ProAla: 1.388 ± 0.63
0.0ProCys: 0.0 ± 0.0
2.949ProAsp: 2.949 ± 0.747
2.082ProGlu: 2.082 ± 0.581
1.214ProPhe: 1.214 ± 0.614
0.173ProGly: 0.173 ± 0.171
0.347ProHis: 0.347 ± 0.245
1.735ProIle: 1.735 ± 0.616
4.511ProLys: 4.511 ± 1.071
3.47ProLeu: 3.47 ± 0.539
0.867ProMet: 0.867 ± 0.318
2.776ProAsn: 2.776 ± 1.317
0.694ProPro: 0.694 ± 0.373
1.561ProGln: 1.561 ± 0.371
0.694ProArg: 0.694 ± 0.41
2.082ProSer: 2.082 ± 0.617
3.296ProThr: 3.296 ± 0.749
2.255ProVal: 2.255 ± 0.569
0.347ProTrp: 0.347 ± 0.24
1.041ProTyr: 1.041 ± 0.426
0.0ProXaa: 0.0 ± 0.0
Gln
1.908GlnAla: 1.908 ± 0.605
0.694GlnCys: 0.694 ± 0.375
2.255GlnAsp: 2.255 ± 0.965
1.561GlnGlu: 1.561 ± 0.572
2.602GlnPhe: 2.602 ± 0.815
2.776GlnGly: 2.776 ± 0.63
0.694GlnHis: 0.694 ± 0.305
3.123GlnIle: 3.123 ± 0.757
2.602GlnLys: 2.602 ± 0.729
4.684GlnLeu: 4.684 ± 0.791
1.561GlnMet: 1.561 ± 0.444
2.776GlnAsn: 2.776 ± 0.615
1.561GlnPro: 1.561 ± 0.792
2.776GlnGln: 2.776 ± 1.058
1.214GlnArg: 1.214 ± 0.388
1.908GlnSer: 1.908 ± 0.53
4.511GlnThr: 4.511 ± 1.203
2.082GlnVal: 2.082 ± 0.561
0.52GlnTrp: 0.52 ± 0.301
1.735GlnTyr: 1.735 ± 0.39
0.0GlnXaa: 0.0 ± 0.0
Arg
1.908ArgAla: 1.908 ± 0.411
0.0ArgCys: 0.0 ± 0.0
1.735ArgAsp: 1.735 ± 0.571
0.867ArgGlu: 0.867 ± 0.575
2.602ArgPhe: 2.602 ± 0.881
1.041ArgGly: 1.041 ± 0.413
0.694ArgHis: 0.694 ± 0.343
2.082ArgIle: 2.082 ± 0.489
2.255ArgLys: 2.255 ± 0.697
2.082ArgLeu: 2.082 ± 0.481
0.52ArgMet: 0.52 ± 0.291
2.255ArgAsn: 2.255 ± 0.745
1.561ArgPro: 1.561 ± 0.37
1.735ArgGln: 1.735 ± 0.589
0.52ArgArg: 0.52 ± 0.355
1.908ArgSer: 1.908 ± 0.485
1.214ArgThr: 1.214 ± 0.552
2.082ArgVal: 2.082 ± 0.762
0.0ArgTrp: 0.0 ± 0.0
2.776ArgTyr: 2.776 ± 0.581
0.0ArgXaa: 0.0 ± 0.0
Ser
4.511SerAla: 4.511 ± 0.592
0.347SerCys: 0.347 ± 0.251
4.164SerAsp: 4.164 ± 1.191
3.817SerGlu: 3.817 ± 0.796
3.123SerPhe: 3.123 ± 0.6
2.949SerGly: 2.949 ± 0.718
0.867SerHis: 0.867 ± 0.273
3.817SerIle: 3.817 ± 0.935
4.511SerLys: 4.511 ± 0.976
3.817SerLeu: 3.817 ± 1.209
1.041SerMet: 1.041 ± 0.365
4.684SerAsn: 4.684 ± 0.999
1.735SerPro: 1.735 ± 0.633
2.602SerGln: 2.602 ± 0.857
1.908SerArg: 1.908 ± 0.53
4.164SerSer: 4.164 ± 0.815
4.337SerThr: 4.337 ± 0.93
2.602SerVal: 2.602 ± 0.569
0.52SerTrp: 0.52 ± 0.342
2.949SerTyr: 2.949 ± 0.729
0.0SerXaa: 0.0 ± 0.0
Thr
5.378ThrAla: 5.378 ± 1.093
0.0ThrCys: 0.0 ± 0.0
3.643ThrAsp: 3.643 ± 0.869
5.552ThrGlu: 5.552 ± 1.402
5.378ThrPhe: 5.378 ± 0.859
4.164ThrGly: 4.164 ± 1.17
0.867ThrHis: 0.867 ± 0.294
6.072ThrIle: 6.072 ± 1.244
4.337ThrLys: 4.337 ± 0.981
7.807ThrLeu: 7.807 ± 0.959
1.735ThrMet: 1.735 ± 0.455
6.94ThrAsn: 6.94 ± 0.726
2.776ThrPro: 2.776 ± 0.428
3.296ThrGln: 3.296 ± 0.655
1.561ThrArg: 1.561 ± 0.728
3.47ThrSer: 3.47 ± 0.762
4.684ThrThr: 4.684 ± 1.241
2.776ThrVal: 2.776 ± 0.61
0.347ThrTrp: 0.347 ± 0.234
3.99ThrTyr: 3.99 ± 0.64
0.0ThrXaa: 0.0 ± 0.0
Val
2.949ValAla: 2.949 ± 0.522
0.173ValCys: 0.173 ± 0.164
3.817ValAsp: 3.817 ± 1.009
4.337ValGlu: 4.337 ± 0.855
2.082ValPhe: 2.082 ± 0.712
3.123ValGly: 3.123 ± 1.219
0.173ValHis: 0.173 ± 0.164
3.99ValIle: 3.99 ± 0.727
4.684ValLys: 4.684 ± 1.198
3.296ValLeu: 3.296 ± 0.686
1.561ValMet: 1.561 ± 0.435
4.858ValAsn: 4.858 ± 0.65
1.735ValPro: 1.735 ± 0.623
1.388ValGln: 1.388 ± 0.369
1.908ValArg: 1.908 ± 0.57
3.296ValSer: 3.296 ± 0.704
4.164ValThr: 4.164 ± 0.934
4.337ValVal: 4.337 ± 1.023
0.52ValTrp: 0.52 ± 0.351
1.388ValTyr: 1.388 ± 0.466
0.0ValXaa: 0.0 ± 0.0
Trp
0.694TrpAla: 0.694 ± 0.437
0.173TrpCys: 0.173 ± 0.171
1.041TrpAsp: 1.041 ± 0.336
0.52TrpGlu: 0.52 ± 0.257
0.694TrpPhe: 0.694 ± 0.255
0.694TrpGly: 0.694 ± 0.36
0.0TrpHis: 0.0 ± 0.0
0.867TrpIle: 0.867 ± 0.318
0.52TrpLys: 0.52 ± 0.274
1.214TrpLeu: 1.214 ± 0.497
0.0TrpMet: 0.0 ± 0.0
0.52TrpAsn: 0.52 ± 0.367
0.0TrpPro: 0.0 ± 0.0
0.173TrpGln: 0.173 ± 0.173
0.173TrpArg: 0.173 ± 0.156
0.52TrpSer: 0.52 ± 0.299
0.867TrpThr: 0.867 ± 0.309
1.041TrpVal: 1.041 ± 0.502
0.0TrpTrp: 0.0 ± 0.0
0.52TrpTyr: 0.52 ± 0.26
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.643TyrAla: 3.643 ± 0.79
0.867TyrCys: 0.867 ± 0.426
3.47TyrAsp: 3.47 ± 0.872
3.296TyrGlu: 3.296 ± 0.788
1.735TyrPhe: 1.735 ± 0.527
2.429TyrGly: 2.429 ± 0.461
0.867TyrHis: 0.867 ± 0.506
3.99TyrIle: 3.99 ± 0.724
3.123TyrLys: 3.123 ± 0.611
3.817TyrLeu: 3.817 ± 0.726
0.867TyrMet: 0.867 ± 0.513
4.858TyrAsn: 4.858 ± 1.022
1.561TyrPro: 1.561 ± 0.496
2.776TyrGln: 2.776 ± 0.497
1.561TyrArg: 1.561 ± 0.572
3.643TyrSer: 3.643 ± 0.887
4.164TyrThr: 4.164 ± 0.713
2.255TyrVal: 2.255 ± 0.573
0.173TyrTrp: 0.173 ± 0.169
2.429TyrTyr: 2.429 ± 0.728
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 24 proteins (5765 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski