Photobacterium lipolyticum
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4228 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T3N529|A0A2T3N529_9GAMM Cytochrome c biogenesis ATP-binding export protein CcmA OS=Photobacterium lipolyticum OX=266810 GN=ccmA PE=3 SV=1
MM1 pKa = 7.65 KK2 pKa = 9.08 KK3 pKa = 8.28 TLLALAVPALLAAGSATAGVNLYY26 pKa = 10.53 DD27 pKa = 4.29 ADD29 pKa = 4.13 GVKK32 pKa = 9.93 TDD34 pKa = 3.97 LSGAAEE40 pKa = 3.9 VQYY43 pKa = 10.54 FQGYY47 pKa = 8.56 EE48 pKa = 4.0 EE49 pKa = 4.87 NSDD52 pKa = 3.13 AKK54 pKa = 10.65 IRR56 pKa = 11.84 LDD58 pKa = 4.96 DD59 pKa = 4.56 GDD61 pKa = 4.38 LAVNTTVAVSGSLNAVAGLAFEE83 pKa = 4.59 MEE85 pKa = 4.64 SGDD88 pKa = 3.74 VTNDD92 pKa = 3.46 EE93 pKa = 4.59 LWVGLAGDD101 pKa = 4.99 FGTLTIGRR109 pKa = 11.84 QYY111 pKa = 11.18 LIADD115 pKa = 3.79 DD116 pKa = 4.15 SGIGKK121 pKa = 10.02 DD122 pKa = 3.81 YY123 pKa = 11.24 EE124 pKa = 4.36 LGGDD128 pKa = 3.52 GLDD131 pKa = 3.33 FVVANGDD138 pKa = 3.33 QTIKK142 pKa = 11.04 YY143 pKa = 9.97 LFDD146 pKa = 3.83 NGQFYY151 pKa = 11.05 AGASALLKK159 pKa = 9.38 EE160 pKa = 4.39 TGKK163 pKa = 10.83 AEE165 pKa = 4.71 DD166 pKa = 3.78 EE167 pKa = 4.41 TTVFDD172 pKa = 3.91 GRR174 pKa = 11.84 LGARR178 pKa = 11.84 FGDD181 pKa = 3.76 LDD183 pKa = 3.69 ARR185 pKa = 11.84 VYY187 pKa = 10.5 LYY189 pKa = 10.92 SSSDD193 pKa = 3.19 VDD195 pKa = 3.48 GGDD198 pKa = 2.86 IFGSVLDD205 pKa = 4.33 GVAIDD210 pKa = 3.8 VEE212 pKa = 4.79 GFNLEE217 pKa = 4.02 AEE219 pKa = 4.46 YY220 pKa = 10.14 IYY222 pKa = 11.19 NAFAFAASFGQVEE235 pKa = 4.75 YY236 pKa = 11.32 KK237 pKa = 10.62 NAANSADD244 pKa = 3.7 KK245 pKa = 11.32 VDD247 pKa = 3.74 VDD249 pKa = 4.08 TAALAGSYY257 pKa = 10.21 TMDD260 pKa = 2.98 RR261 pKa = 11.84 TTFAVGYY268 pKa = 8.18 TYY270 pKa = 11.38 LDD272 pKa = 4.12 FEE274 pKa = 7.01 ADD276 pKa = 3.77 DD277 pKa = 3.86 VSAFYY282 pKa = 11.46 ANVTQQLHH290 pKa = 6.38 SNVKK294 pKa = 8.9 VYY296 pKa = 11.26 GEE298 pKa = 4.53 IGSSDD303 pKa = 3.42 ADD305 pKa = 3.3 NSEE308 pKa = 4.16 FGYY311 pKa = 9.59 VAGMEE316 pKa = 4.3 VSFF319 pKa = 5.08
Molecular weight: 33.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.516
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.414
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.859
Patrickios 1.875
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A2T3MSZ1|A0A2T3MSZ1_9GAMM ATP-dependent protease OS=Photobacterium lipolyticum OX=266810 GN=C9I89_20030 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 ATINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4228
0
4228
1369385
26
2525
323.9
35.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.862 ± 0.042
1.123 ± 0.013
5.591 ± 0.035
6.251 ± 0.038
3.98 ± 0.028
6.911 ± 0.037
2.252 ± 0.018
6.407 ± 0.029
5.137 ± 0.032
10.442 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.711 ± 0.021
4.149 ± 0.028
3.996 ± 0.021
4.585 ± 0.034
4.742 ± 0.031
6.349 ± 0.033
5.375 ± 0.023
6.941 ± 0.037
1.227 ± 0.016
2.97 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here