Primorskyibacter insulae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudoprimorskyibacter

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3838 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R8AWS6|A0A2R8AWS6_9RHOB Putative phosphoadenosine phosphosulfate reductase OS=Primorskyibacter insulae OX=1695997 GN=cysH PE=3 SV=1
MM1 pKa = 7.86ASPAFATFTNSLSGNYY17 pKa = 10.24DD18 pKa = 3.1EE19 pKa = 6.41DD20 pKa = 5.38RR21 pKa = 11.84YY22 pKa = 10.79ILQLTAGKK30 pKa = 10.18NYY32 pKa = 9.8WYY34 pKa = 9.96DD35 pKa = 3.37LEE37 pKa = 4.9RR38 pKa = 11.84AVDD41 pKa = 3.87DD42 pKa = 4.35PGGISVSFASQTGARR57 pKa = 11.84MDD59 pKa = 4.21GNTATADD66 pKa = 3.43GDD68 pKa = 3.68HH69 pKa = 6.79RR70 pKa = 11.84LVLKK74 pKa = 9.15SQPYY78 pKa = 10.95AMYY81 pKa = 9.31TPNYY85 pKa = 7.9VPGYY89 pKa = 9.37AGAYY93 pKa = 5.82TLSVYY98 pKa = 10.84EE99 pKa = 4.12EE100 pKa = 4.32VGNSNASTEE109 pKa = 4.06RR110 pKa = 11.84LEE112 pKa = 5.13VEE114 pKa = 4.03TAYY117 pKa = 10.37HH118 pKa = 6.37GRR120 pKa = 11.84FDD122 pKa = 3.98YY123 pKa = 11.42VSDD126 pKa = 3.75RR127 pKa = 11.84DD128 pKa = 3.86VIGFQGRR135 pKa = 11.84AGVGYY140 pKa = 9.88FNEE143 pKa = 4.09IVTEE147 pKa = 4.16VEE149 pKa = 4.02GATVSGYY156 pKa = 10.24IKK158 pKa = 10.62NDD160 pKa = 2.96GSGPSYY166 pKa = 10.86TYY168 pKa = 7.34FTPEE172 pKa = 4.07ADD174 pKa = 3.2EE175 pKa = 4.11LLYY178 pKa = 11.09VGVYY182 pKa = 9.97SGVVTDD188 pKa = 3.41YY189 pKa = 10.73TLTVRR194 pKa = 11.84QEE196 pKa = 4.06VGNSLSSVAVLKK208 pKa = 10.37PDD210 pKa = 3.2TSIASRR216 pKa = 11.84IDD218 pKa = 3.18YY219 pKa = 9.96MGDD222 pKa = 2.97YY223 pKa = 10.91DD224 pKa = 4.26VFHH227 pKa = 6.3VTPEE231 pKa = 4.16DD232 pKa = 3.89GFGLTVTVTAADD244 pKa = 3.71TDD246 pKa = 4.14PALGLDD252 pKa = 4.14LFAYY256 pKa = 9.72EE257 pKa = 4.33YY258 pKa = 10.64DD259 pKa = 3.37VSYY262 pKa = 11.14GVTDD266 pKa = 3.55TQFVLTGPTAITVGADD282 pKa = 3.43GPGGYY287 pKa = 9.4ILTAEE292 pKa = 4.21RR293 pKa = 11.84EE294 pKa = 4.37VLGTLDD300 pKa = 4.29SKK302 pKa = 12.11SMLTPDD308 pKa = 4.29APVHH312 pKa = 6.42DD313 pKa = 4.11TLQYY317 pKa = 11.2GGDD320 pKa = 3.06IDD322 pKa = 3.88VFRR325 pKa = 11.84FEE327 pKa = 4.38ITDD330 pKa = 3.63GADD333 pKa = 2.99YY334 pKa = 10.81LIRR337 pKa = 11.84FRR339 pKa = 11.84GDD341 pKa = 2.99PGGDD345 pKa = 2.78EE346 pKa = 4.15LTRR349 pKa = 11.84LRR351 pKa = 11.84FAIFDD356 pKa = 3.69EE357 pKa = 5.93DD358 pKa = 3.99GTQVPVNGGLGSEE371 pKa = 3.78WRR373 pKa = 11.84YY374 pKa = 8.18LTEE377 pKa = 4.18GLGAGTYY384 pKa = 8.27FARR387 pKa = 11.84LLTGQIYY394 pKa = 10.71DD395 pKa = 3.6GGDD398 pKa = 3.67YY399 pKa = 10.45ILTMTPEE406 pKa = 4.19EE407 pKa = 4.62AGDD410 pKa = 3.87AQTGSAIRR418 pKa = 11.84PGGQFAGNIDD428 pKa = 3.45EE429 pKa = 5.08WDD431 pKa = 3.85DD432 pKa = 3.8EE433 pKa = 4.41DD434 pKa = 3.44WVRR437 pKa = 11.84IVAQPGRR444 pKa = 11.84DD445 pKa = 3.35VTLSIDD451 pKa = 3.67MGSGAPTLSPIEE463 pKa = 4.12PEE465 pKa = 3.71EE466 pKa = 4.72GIFLSLVDD474 pKa = 3.58EE475 pKa = 4.87TGTVLAISTVTDD487 pKa = 3.36TSEE490 pKa = 4.09TLTFTPEE497 pKa = 3.55PGKK500 pKa = 9.46TYY502 pKa = 9.83FAKK505 pKa = 9.41VTPPPGSFSLFGPDD519 pKa = 2.16IFAWSEE525 pKa = 3.31GDD527 pKa = 2.93MGYY530 pKa = 11.02GYY532 pKa = 8.39TLSMTQNGVRR542 pKa = 11.84IGTALAEE549 pKa = 4.29VLSGSDD555 pKa = 3.43GPDD558 pKa = 3.28WITADD563 pKa = 4.08AGNDD567 pKa = 3.53TVNALDD573 pKa = 4.39GADD576 pKa = 3.54TVIAGPGDD584 pKa = 3.61DD585 pKa = 3.94VVNGGTTAADD595 pKa = 3.77LRR597 pKa = 11.84DD598 pKa = 3.91VIYY601 pKa = 10.72GGDD604 pKa = 3.78GDD606 pKa = 4.49DD607 pKa = 4.4SLVGGYY613 pKa = 10.89GNDD616 pKa = 3.67EE617 pKa = 4.11LRR619 pKa = 11.84GDD621 pKa = 4.01AGNDD625 pKa = 3.31TLEE628 pKa = 4.58GGFGADD634 pKa = 3.12TVLGGTGNDD643 pKa = 3.56VLTGGAWGDD652 pKa = 3.57MLFGGDD658 pKa = 5.18GDD660 pKa = 5.0DD661 pKa = 4.1FLNGGFGFDD670 pKa = 3.81RR671 pKa = 11.84LNGGSGADD679 pKa = 3.32QFFHH683 pKa = 7.11LGAAGHH689 pKa = 6.09GSDD692 pKa = 4.83WIQDD696 pKa = 3.36YY697 pKa = 10.93SAAEE701 pKa = 4.1GDD703 pKa = 3.81VLVYY707 pKa = 10.87GGTATKK713 pKa = 10.91GDD715 pKa = 4.08FLVQRR720 pKa = 11.84AATPGAGDD728 pKa = 3.55AAVKK732 pKa = 10.04EE733 pKa = 4.43VFITHH738 pKa = 5.85VPSNALLWALVDD750 pKa = 4.18GDD752 pKa = 4.02AQAAITVRR760 pKa = 11.84AGGVEE765 pKa = 4.07FDD767 pKa = 4.56LLAA770 pKa = 5.47

Molecular weight: 80.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R8APN7|A0A2R8APN7_9RHOB 4 5-dihydroxyphthalate dehydrogenase OS=Primorskyibacter insulae OX=1695997 GN=pht4 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3838

0

3838

1215343

29

9594

316.7

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.074 ± 0.061

0.869 ± 0.015

6.48 ± 0.05

5.616 ± 0.044

3.698 ± 0.029

8.81 ± 0.074

2.024 ± 0.022

5.223 ± 0.026

3.421 ± 0.036

9.83 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.83 ± 0.027

2.826 ± 0.03

4.907 ± 0.039

3.368 ± 0.025

6.28 ± 0.053

5.2 ± 0.036

5.686 ± 0.056

7.24 ± 0.042

1.345 ± 0.018

2.273 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski