Mycobacterium phage Henu3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410T7H7|A0A410T7H7_9CAUD HNH endonuclease OS=Mycobacterium phage Henu3 OX=2492961 GN=Henu3_gp25 PE=4 SV=1
MM1 pKa = 8.44IEE3 pKa = 3.88EE4 pKa = 4.17TTYY7 pKa = 10.74RR8 pKa = 11.84AARR11 pKa = 11.84LDD13 pKa = 3.67AEE15 pKa = 4.79TIDD18 pKa = 5.44DD19 pKa = 4.4LAALPSSSITYY30 pKa = 10.04DD31 pKa = 4.74DD32 pKa = 4.26PQADD36 pKa = 4.01NNTRR40 pKa = 11.84AAFAAEE46 pKa = 3.78ALVAYY51 pKa = 8.36VRR53 pKa = 11.84RR54 pKa = 11.84VGDD57 pKa = 3.71PGEE60 pKa = 4.7LEE62 pKa = 4.16TAVTDD67 pKa = 3.72LLGDD71 pKa = 3.88LRR73 pKa = 11.84HH74 pKa = 6.41LCDD77 pKa = 3.82ALGFDD82 pKa = 4.95FEE84 pKa = 5.25DD85 pKa = 3.7AAEE88 pKa = 4.12MSFCHH93 pKa = 6.6YY94 pKa = 10.24DD95 pKa = 3.15AEE97 pKa = 4.98IRR99 pKa = 11.84GEE101 pKa = 4.02LL102 pKa = 3.7

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410T7W2|A0A410T7W2_9CAUD Terminase OS=Mycobacterium phage Henu3 OX=2492961 GN=Henu3_gp98 PE=4 SV=1
MM1 pKa = 6.55ATKK4 pKa = 10.25RR5 pKa = 11.84RR6 pKa = 11.84APGEE10 pKa = 3.91GGLFKK15 pKa = 10.55RR16 pKa = 11.84ADD18 pKa = 3.79GMWVGRR24 pKa = 11.84VDD26 pKa = 3.97VPTADD31 pKa = 2.81GRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84VVYY40 pKa = 10.75SRR42 pKa = 11.84DD43 pKa = 3.23KK44 pKa = 11.22AVAAEE49 pKa = 4.18KK50 pKa = 10.47LRR52 pKa = 11.84KK53 pKa = 9.2LRR55 pKa = 11.84NDD57 pKa = 3.06VAEE60 pKa = 4.55GRR62 pKa = 11.84VSSSPTMTLGAWLDD76 pKa = 3.35QWLDD80 pKa = 2.82IHH82 pKa = 6.56GPHH85 pKa = 6.17VRR87 pKa = 11.84PTTRR91 pKa = 11.84RR92 pKa = 11.84HH93 pKa = 5.6HH94 pKa = 5.5EE95 pKa = 3.65QSIRR99 pKa = 11.84LYY101 pKa = 9.42IKK103 pKa = 9.21PHH105 pKa = 5.85ISDD108 pKa = 3.39RR109 pKa = 11.84RR110 pKa = 11.84LDD112 pKa = 4.25KK113 pKa = 10.73LTLDD117 pKa = 4.51DD118 pKa = 3.95VEE120 pKa = 4.88KK121 pKa = 10.33MLRR124 pKa = 11.84AVAARR129 pKa = 11.84STKK132 pKa = 10.06SAQKK136 pKa = 10.43AHH138 pKa = 5.13QTLSQALKK146 pKa = 7.86EE147 pKa = 4.15AEE149 pKa = 4.01RR150 pKa = 11.84RR151 pKa = 11.84GLVYY155 pKa = 10.58RR156 pKa = 11.84NVAAIVRR163 pKa = 11.84KK164 pKa = 8.8PRR166 pKa = 11.84HH167 pKa = 5.76APRR170 pKa = 11.84QRR172 pKa = 11.84EE173 pKa = 4.2ALTVDD178 pKa = 3.44QARR181 pKa = 11.84HH182 pKa = 5.87IISTAIDD189 pKa = 3.98LEE191 pKa = 4.65PAAGPHH197 pKa = 6.71LATRR201 pKa = 11.84WAAAFLTGARR211 pKa = 11.84QAEE214 pKa = 4.43LLGLTWDD221 pKa = 4.9RR222 pKa = 11.84VDD224 pKa = 5.37LDD226 pKa = 4.21AGTIDD231 pKa = 5.79LAWQLQQLQHH241 pKa = 4.66VHH243 pKa = 6.13GCGEE247 pKa = 4.22PDD249 pKa = 5.91DD250 pKa = 5.62DD251 pKa = 5.99GEE253 pKa = 4.5WPCGRR258 pKa = 11.84VRR260 pKa = 11.84PGWCPSRR267 pKa = 11.84RR268 pKa = 11.84WEE270 pKa = 4.03LPAGFEE276 pKa = 4.36YY277 pKa = 10.72KK278 pKa = 10.17VVHH281 pKa = 6.74RR282 pKa = 11.84SLAFTRR288 pKa = 11.84PKK290 pKa = 9.39TRR292 pKa = 11.84AGTRR296 pKa = 11.84VVPLAEE302 pKa = 4.68PLRR305 pKa = 11.84LMLVEE310 pKa = 3.86HH311 pKa = 7.39AKK313 pKa = 10.55RR314 pKa = 11.84SGPNPHH320 pKa = 6.25NLVWRR325 pKa = 11.84YY326 pKa = 9.57PDD328 pKa = 3.55GRR330 pKa = 11.84PIGPRR335 pKa = 11.84DD336 pKa = 3.92DD337 pKa = 3.35LHH339 pKa = 6.55AWRR342 pKa = 11.84VLLAAAGVDD351 pKa = 3.86PVPLHH356 pKa = 5.69SARR359 pKa = 11.84HH360 pKa = 4.18TTATLLLAAGVDD372 pKa = 3.53AHH374 pKa = 6.57VVSAILGHH382 pKa = 6.12SDD384 pKa = 3.66VIVTRR389 pKa = 11.84GYY391 pKa = 9.2QHH393 pKa = 7.12VDD395 pKa = 2.93LTLARR400 pKa = 11.84QAAGQLTRR408 pKa = 11.84LLSS411 pKa = 3.63

Molecular weight: 45.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

21941

67

1325

255.1

27.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.702 ± 0.376

1.244 ± 0.117

5.907 ± 0.236

4.772 ± 0.281

2.247 ± 0.196

8.514 ± 0.428

2.94 ± 0.292

3.382 ± 0.242

2.293 ± 0.227

8.655 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.582 ± 0.11

2.475 ± 0.181

6.572 ± 0.285

3.778 ± 0.198

10.15 ± 0.717

5.834 ± 0.36

5.383 ± 0.315

8.322 ± 0.318

1.49 ± 0.137

1.759 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski