Amino acid dipepetide frequency for Mycobacterium phage Henu3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.69AlaAla: 19.69 ± 1.416
1.231AlaCys: 1.231 ± 0.345
7.521AlaAsp: 7.521 ± 0.598
8.204AlaGlu: 8.204 ± 1.097
2.598AlaPhe: 2.598 ± 0.538
10.665AlaGly: 10.665 ± 0.903
2.963AlaHis: 2.963 ± 0.396
4.284AlaIle: 4.284 ± 0.56
2.552AlaLys: 2.552 ± 0.31
10.757AlaLeu: 10.757 ± 0.766
2.188AlaMet: 2.188 ± 0.39
2.598AlaAsn: 2.598 ± 0.369
7.429AlaPro: 7.429 ± 0.915
5.469AlaGln: 5.469 ± 0.445
10.392AlaArg: 10.392 ± 0.958
6.7AlaSer: 6.7 ± 0.826
6.062AlaThr: 6.062 ± 0.639
11.851AlaVal: 11.851 ± 0.919
1.823AlaTrp: 1.823 ± 0.395
2.051AlaTyr: 2.051 ± 0.328
0.0AlaXaa: 0.0 ± 0.0
Cys
1.641CysAla: 1.641 ± 0.337
0.319CysCys: 0.319 ± 0.119
0.684CysAsp: 0.684 ± 0.18
0.501CysGlu: 0.501 ± 0.146
0.319CysPhe: 0.319 ± 0.117
1.413CysGly: 1.413 ± 0.312
0.228CysHis: 0.228 ± 0.087
0.501CysIle: 0.501 ± 0.156
0.228CysLys: 0.228 ± 0.121
0.501CysLeu: 0.501 ± 0.176
0.137CysMet: 0.137 ± 0.071
0.091CysAsn: 0.091 ± 0.06
1.003CysPro: 1.003 ± 0.237
0.273CysGln: 0.273 ± 0.11
1.504CysArg: 1.504 ± 0.319
1.231CysSer: 1.231 ± 0.252
0.82CysThr: 0.82 ± 0.226
0.729CysVal: 0.729 ± 0.254
0.273CysTrp: 0.273 ± 0.12
0.046CysTyr: 0.046 ± 0.044
0.0CysXaa: 0.0 ± 0.0
Asp
6.882AspAla: 6.882 ± 0.576
0.41AspCys: 0.41 ± 0.137
5.287AspAsp: 5.287 ± 0.782
4.649AspGlu: 4.649 ± 0.474
1.322AspPhe: 1.322 ± 0.266
5.697AspGly: 5.697 ± 0.565
1.139AspHis: 1.139 ± 0.258
1.048AspIle: 1.048 ± 0.222
1.276AspLys: 1.276 ± 0.31
5.059AspLeu: 5.059 ± 0.548
1.231AspMet: 1.231 ± 0.301
1.322AspAsn: 1.322 ± 0.281
4.057AspPro: 4.057 ± 0.456
1.595AspGln: 1.595 ± 0.227
6.381AspArg: 6.381 ± 0.657
3.054AspSer: 3.054 ± 0.504
2.689AspThr: 2.689 ± 0.292
4.603AspVal: 4.603 ± 0.575
0.638AspTrp: 0.638 ± 0.149
0.729AspTyr: 0.729 ± 0.161
0.0AspXaa: 0.0 ± 0.0
Glu
5.652GluAla: 5.652 ± 0.788
0.501GluCys: 0.501 ± 0.155
1.96GluAsp: 1.96 ± 0.332
1.139GluGlu: 1.139 ± 0.292
0.82GluPhe: 0.82 ± 0.209
2.735GluGly: 2.735 ± 0.364
2.279GluHis: 2.279 ± 0.298
1.504GluIle: 1.504 ± 0.313
1.595GluLys: 1.595 ± 0.315
6.335GluLeu: 6.335 ± 0.623
0.912GluMet: 0.912 ± 0.182
0.957GluAsn: 0.957 ± 0.199
3.829GluPro: 3.829 ± 0.464
2.416GluGln: 2.416 ± 0.359
4.786GluArg: 4.786 ± 0.765
1.823GluSer: 1.823 ± 0.33
2.325GluThr: 2.325 ± 0.306
4.376GluVal: 4.376 ± 0.588
0.729GluTrp: 0.729 ± 0.158
1.048GluTyr: 1.048 ± 0.19
0.0GluXaa: 0.0 ± 0.0
Phe
2.507PheAla: 2.507 ± 0.312
0.182PheCys: 0.182 ± 0.091
2.188PheAsp: 2.188 ± 0.297
1.367PheGlu: 1.367 ± 0.25
0.456PhePhe: 0.456 ± 0.148
2.416PheGly: 2.416 ± 0.365
0.547PheHis: 0.547 ± 0.131
0.866PheIle: 0.866 ± 0.188
0.593PheLys: 0.593 ± 0.197
1.778PheLeu: 1.778 ± 0.27
0.593PheMet: 0.593 ± 0.166
0.684PheAsn: 0.684 ± 0.207
0.82PhePro: 0.82 ± 0.222
0.82PheGln: 0.82 ± 0.154
1.322PheArg: 1.322 ± 0.241
1.413PheSer: 1.413 ± 0.239
1.504PheThr: 1.504 ± 0.29
1.823PheVal: 1.823 ± 0.242
0.365PheTrp: 0.365 ± 0.13
0.228PheTyr: 0.228 ± 0.098
0.0PheXaa: 0.0 ± 0.0
Gly
9.025GlyAla: 9.025 ± 0.818
0.957GlyCys: 0.957 ± 0.195
5.697GlyAsp: 5.697 ± 0.695
4.877GlyGlu: 4.877 ± 0.52
1.914GlyPhe: 1.914 ± 0.327
8.25GlyGly: 8.25 ± 0.972
2.78GlyHis: 2.78 ± 0.477
2.552GlyIle: 2.552 ± 0.439
2.325GlyLys: 2.325 ± 0.349
6.791GlyLeu: 6.791 ± 0.767
1.55GlyMet: 1.55 ± 0.272
2.644GlyAsn: 2.644 ± 0.486
3.418GlyPro: 3.418 ± 0.539
4.193GlyGln: 4.193 ± 0.473
8.933GlyArg: 8.933 ± 0.839
5.15GlySer: 5.15 ± 0.705
4.512GlyThr: 4.512 ± 0.608
6.244GlyVal: 6.244 ± 0.659
1.276GlyTrp: 1.276 ± 0.297
2.005GlyTyr: 2.005 ± 0.358
0.0GlyXaa: 0.0 ± 0.0
His
2.552HisAla: 2.552 ± 0.342
0.228HisCys: 0.228 ± 0.096
1.641HisAsp: 1.641 ± 0.284
1.322HisGlu: 1.322 ± 0.257
0.684HisPhe: 0.684 ± 0.176
2.416HisGly: 2.416 ± 0.38
1.185HisHis: 1.185 ± 0.218
1.185HisIle: 1.185 ± 0.266
0.456HisLys: 0.456 ± 0.153
2.963HisLeu: 2.963 ± 0.481
0.501HisMet: 0.501 ± 0.118
0.547HisAsn: 0.547 ± 0.149
1.322HisPro: 1.322 ± 0.274
1.231HisGln: 1.231 ± 0.219
4.284HisArg: 4.284 ± 0.716
1.732HisSer: 1.732 ± 0.301
1.048HisThr: 1.048 ± 0.234
3.464HisVal: 3.464 ± 0.493
0.273HisTrp: 0.273 ± 0.103
0.365HisTyr: 0.365 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
4.421IleAla: 4.421 ± 0.513
0.228IleCys: 0.228 ± 0.111
2.279IleAsp: 2.279 ± 0.417
2.188IleGlu: 2.188 ± 0.398
0.684IlePhe: 0.684 ± 0.184
4.148IleGly: 4.148 ± 0.564
0.729IleHis: 0.729 ± 0.159
1.048IleIle: 1.048 ± 0.263
1.048IleLys: 1.048 ± 0.431
2.142IleLeu: 2.142 ± 0.274
0.319IleMet: 0.319 ± 0.11
1.094IleAsn: 1.094 ± 0.264
1.778IlePro: 1.778 ± 0.281
0.684IleGln: 0.684 ± 0.226
2.552IleArg: 2.552 ± 0.388
1.778IleSer: 1.778 ± 0.27
2.644IleThr: 2.644 ± 0.427
2.917IleVal: 2.917 ± 0.418
0.456IleTrp: 0.456 ± 0.176
0.684IleTyr: 0.684 ± 0.186
0.0IleXaa: 0.0 ± 0.0
Lys
3.191LysAla: 3.191 ± 0.485
0.41LysCys: 0.41 ± 0.126
1.139LysAsp: 1.139 ± 0.344
0.638LysGlu: 0.638 ± 0.162
0.41LysPhe: 0.41 ± 0.13
1.504LysGly: 1.504 ± 0.244
0.684LysHis: 0.684 ± 0.206
0.912LysIle: 0.912 ± 0.203
0.365LysLys: 0.365 ± 0.221
2.097LysLeu: 2.097 ± 0.287
0.866LysMet: 0.866 ± 0.204
0.775LysAsn: 0.775 ± 0.172
1.595LysPro: 1.595 ± 0.257
1.003LysGln: 1.003 ± 0.229
1.778LysArg: 1.778 ± 0.306
1.459LysSer: 1.459 ± 0.261
1.322LysThr: 1.322 ± 0.385
1.914LysVal: 1.914 ± 0.305
0.41LysTrp: 0.41 ± 0.137
0.456LysTyr: 0.456 ± 0.146
0.0LysXaa: 0.0 ± 0.0
Leu
12.124LeuAla: 12.124 ± 0.879
0.729LeuCys: 0.729 ± 0.173
6.518LeuAsp: 6.518 ± 0.64
1.732LeuGlu: 1.732 ± 0.29
1.778LeuPhe: 1.778 ± 0.296
8.751LeuGly: 8.751 ± 0.651
2.37LeuHis: 2.37 ± 0.421
2.826LeuIle: 2.826 ± 0.377
1.914LeuLys: 1.914 ± 0.394
7.156LeuLeu: 7.156 ± 0.67
1.185LeuMet: 1.185 ± 0.229
2.097LeuAsn: 2.097 ± 0.335
5.652LeuPro: 5.652 ± 0.54
3.327LeuGln: 3.327 ± 0.405
8.751LeuArg: 8.751 ± 0.739
4.786LeuSer: 4.786 ± 0.541
4.695LeuThr: 4.695 ± 0.501
7.748LeuVal: 7.748 ± 0.725
0.912LeuTrp: 0.912 ± 0.287
1.504LeuTyr: 1.504 ± 0.282
0.0LeuXaa: 0.0 ± 0.0
Met
1.276MetAla: 1.276 ± 0.253
0.137MetCys: 0.137 ± 0.079
0.456MetAsp: 0.456 ± 0.13
0.273MetGlu: 0.273 ± 0.107
0.593MetPhe: 0.593 ± 0.205
0.729MetGly: 0.729 ± 0.163
0.638MetHis: 0.638 ± 0.173
0.684MetIle: 0.684 ± 0.169
0.638MetLys: 0.638 ± 0.177
1.823MetLeu: 1.823 ± 0.279
0.273MetMet: 0.273 ± 0.111
0.547MetAsn: 0.547 ± 0.159
1.185MetPro: 1.185 ± 0.298
0.41MetGln: 0.41 ± 0.145
1.367MetArg: 1.367 ± 0.237
1.869MetSer: 1.869 ± 0.378
1.55MetThr: 1.55 ± 0.258
1.048MetVal: 1.048 ± 0.214
0.273MetTrp: 0.273 ± 0.115
0.046MetTyr: 0.046 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
3.373AsnAla: 3.373 ± 0.449
0.273AsnCys: 0.273 ± 0.119
1.276AsnAsp: 1.276 ± 0.228
1.048AsnGlu: 1.048 ± 0.195
0.729AsnPhe: 0.729 ± 0.182
2.097AsnGly: 2.097 ± 0.332
0.593AsnHis: 0.593 ± 0.175
0.775AsnIle: 0.775 ± 0.249
0.456AsnLys: 0.456 ± 0.135
2.598AsnLeu: 2.598 ± 0.375
0.501AsnMet: 0.501 ± 0.137
0.501AsnAsn: 0.501 ± 0.141
2.325AsnPro: 2.325 ± 0.356
0.547AsnGln: 0.547 ± 0.169
1.778AsnArg: 1.778 ± 0.323
1.231AsnSer: 1.231 ± 0.291
1.595AsnThr: 1.595 ± 0.277
2.279AsnVal: 2.279 ± 0.365
0.365AsnTrp: 0.365 ± 0.121
0.41AsnTyr: 0.41 ± 0.137
0.0AsnXaa: 0.0 ± 0.0
Pro
9.298ProAla: 9.298 ± 1.022
0.456ProCys: 0.456 ± 0.106
2.826ProAsp: 2.826 ± 0.373
3.008ProGlu: 3.008 ± 0.51
1.185ProPhe: 1.185 ± 0.233
7.384ProGly: 7.384 ± 0.587
2.325ProHis: 2.325 ± 0.392
2.461ProIle: 2.461 ± 0.354
1.139ProLys: 1.139 ± 0.206
4.877ProLeu: 4.877 ± 0.444
0.684ProMet: 0.684 ± 0.228
1.686ProAsn: 1.686 ± 0.265
5.378ProPro: 5.378 ± 0.663
1.914ProGln: 1.914 ± 0.326
6.928ProArg: 6.928 ± 0.823
3.737ProSer: 3.737 ± 0.489
3.737ProThr: 3.737 ± 0.406
4.923ProVal: 4.923 ± 0.527
0.547ProTrp: 0.547 ± 0.131
1.231ProTyr: 1.231 ± 0.265
0.0ProXaa: 0.0 ± 0.0
Gln
4.923GlnAla: 4.923 ± 0.497
0.593GlnCys: 0.593 ± 0.155
1.139GlnAsp: 1.139 ± 0.249
1.231GlnGlu: 1.231 ± 0.258
0.957GlnPhe: 0.957 ± 0.184
1.55GlnGly: 1.55 ± 0.269
1.778GlnHis: 1.778 ± 0.379
1.55GlnIle: 1.55 ± 0.24
1.003GlnLys: 1.003 ± 0.2
4.284GlnLeu: 4.284 ± 0.593
0.547GlnMet: 0.547 ± 0.165
0.957GlnAsn: 0.957 ± 0.184
3.51GlnPro: 3.51 ± 0.456
2.233GlnGln: 2.233 ± 0.368
4.968GlnArg: 4.968 ± 0.862
1.367GlnSer: 1.367 ± 0.235
1.459GlnThr: 1.459 ± 0.301
2.507GlnVal: 2.507 ± 0.368
0.593GlnTrp: 0.593 ± 0.144
0.638GlnTyr: 0.638 ± 0.164
0.0GlnXaa: 0.0 ± 0.0
Arg
11.531ArgAla: 11.531 ± 1.201
2.735ArgCys: 2.735 ± 0.47
4.786ArgAsp: 4.786 ± 0.569
3.92ArgGlu: 3.92 ± 0.429
2.279ArgPhe: 2.279 ± 0.288
5.652ArgGly: 5.652 ± 0.689
4.148ArgHis: 4.148 ± 0.595
3.51ArgIle: 3.51 ± 0.442
1.96ArgLys: 1.96 ± 0.364
9.298ArgLeu: 9.298 ± 0.88
1.686ArgMet: 1.686 ± 0.287
2.552ArgAsn: 2.552 ± 0.318
6.928ArgPro: 6.928 ± 0.879
4.923ArgGln: 4.923 ± 0.555
15.178ArgArg: 15.178 ± 1.822
6.29ArgSer: 6.29 ± 0.836
3.464ArgThr: 3.464 ± 0.369
7.247ArgVal: 7.247 ± 0.679
1.686ArgTrp: 1.686 ± 0.37
1.732ArgTyr: 1.732 ± 0.322
0.0ArgXaa: 0.0 ± 0.0
Ser
8.204SerAla: 8.204 ± 0.918
0.912SerCys: 0.912 ± 0.236
2.644SerAsp: 2.644 ± 0.361
1.641SerGlu: 1.641 ± 0.301
1.914SerPhe: 1.914 ± 0.305
5.242SerGly: 5.242 ± 0.742
1.231SerHis: 1.231 ± 0.243
1.686SerIle: 1.686 ± 0.299
1.003SerLys: 1.003 ± 0.202
3.737SerLeu: 3.737 ± 0.478
0.82SerMet: 0.82 ± 0.187
1.595SerAsn: 1.595 ± 0.381
3.692SerPro: 3.692 ± 0.468
1.322SerGln: 1.322 ± 0.249
5.834SerArg: 5.834 ± 0.743
5.606SerSer: 5.606 ± 0.861
4.33SerThr: 4.33 ± 0.633
4.467SerVal: 4.467 ± 0.397
1.094SerTrp: 1.094 ± 0.219
1.367SerTyr: 1.367 ± 0.257
0.0SerXaa: 0.0 ± 0.0
Thr
6.563ThrAla: 6.563 ± 0.755
0.775ThrCys: 0.775 ± 0.184
2.552ThrAsp: 2.552 ± 0.365
2.917ThrGlu: 2.917 ± 0.355
1.732ThrPhe: 1.732 ± 0.262
5.287ThrGly: 5.287 ± 0.708
1.276ThrHis: 1.276 ± 0.261
2.963ThrIle: 2.963 ± 0.43
1.504ThrLys: 1.504 ± 0.262
4.193ThrLeu: 4.193 ± 0.542
0.593ThrMet: 0.593 ± 0.171
1.185ThrAsn: 1.185 ± 0.214
4.376ThrPro: 4.376 ± 0.434
1.413ThrGln: 1.413 ± 0.29
4.057ThrArg: 4.057 ± 0.525
2.644ThrSer: 2.644 ± 0.438
3.327ThrThr: 3.327 ± 0.506
4.421ThrVal: 4.421 ± 0.525
1.003ThrTrp: 1.003 ± 0.21
1.048ThrTyr: 1.048 ± 0.232
0.0ThrXaa: 0.0 ± 0.0
Val
11.03ValAla: 11.03 ± 0.903
0.684ValCys: 0.684 ± 0.194
6.837ValAsp: 6.837 ± 0.629
5.469ValGlu: 5.469 ± 0.587
1.322ValPhe: 1.322 ± 0.239
6.563ValGly: 6.563 ± 0.636
1.96ValHis: 1.96 ± 0.458
2.598ValIle: 2.598 ± 0.37
1.686ValLys: 1.686 ± 0.366
7.247ValLeu: 7.247 ± 0.794
0.775ValMet: 0.775 ± 0.18
1.914ValAsn: 1.914 ± 0.313
5.606ValPro: 5.606 ± 0.506
2.552ValGln: 2.552 ± 0.343
6.882ValArg: 6.882 ± 0.576
4.33ValSer: 4.33 ± 0.476
4.558ValThr: 4.558 ± 0.38
8.341ValVal: 8.341 ± 0.774
1.413ValTrp: 1.413 ± 0.235
1.459ValTyr: 1.459 ± 0.233
0.0ValXaa: 0.0 ± 0.0
Trp
1.139TrpAla: 1.139 ± 0.234
0.365TrpCys: 0.365 ± 0.103
0.547TrpAsp: 0.547 ± 0.13
0.729TrpGlu: 0.729 ± 0.186
0.593TrpPhe: 0.593 ± 0.15
0.82TrpGly: 0.82 ± 0.247
0.365TrpHis: 0.365 ± 0.127
0.273TrpIle: 0.273 ± 0.098
0.365TrpLys: 0.365 ± 0.122
1.55TrpLeu: 1.55 ± 0.286
0.228TrpMet: 0.228 ± 0.086
0.456TrpAsn: 0.456 ± 0.187
0.866TrpPro: 0.866 ± 0.214
0.957TrpGln: 0.957 ± 0.179
2.142TrpArg: 2.142 ± 0.306
0.957TrpSer: 0.957 ± 0.218
1.094TrpThr: 1.094 ± 0.249
0.912TrpVal: 0.912 ± 0.233
0.319TrpTrp: 0.319 ± 0.113
0.228TrpTyr: 0.228 ± 0.089
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.005TyrAla: 2.005 ± 0.321
0.319TyrCys: 0.319 ± 0.132
1.094TyrAsp: 1.094 ± 0.269
0.866TyrGlu: 0.866 ± 0.237
0.182TyrPhe: 0.182 ± 0.082
1.823TyrGly: 1.823 ± 0.321
0.182TyrHis: 0.182 ± 0.096
0.593TyrIle: 0.593 ± 0.162
0.82TyrLys: 0.82 ± 0.236
1.367TyrLeu: 1.367 ± 0.305
0.182TyrMet: 0.182 ± 0.078
0.547TyrAsn: 0.547 ± 0.147
0.957TyrPro: 0.957 ± 0.259
0.501TyrGln: 0.501 ± 0.186
1.686TyrArg: 1.686 ± 0.26
1.185TyrSer: 1.185 ± 0.21
1.094TyrThr: 1.094 ± 0.237
1.413TyrVal: 1.413 ± 0.264
0.456TyrTrp: 0.456 ± 0.135
0.319TyrTyr: 0.319 ± 0.121
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (21941 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski