Pseudaminobacter manganicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Pseudaminobacter

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V8RQ89|A0A1V8RQ89_9RHIZ Uncharacterized protein OS=Pseudaminobacter manganicus OX=1873176 GN=BFN67_18905 PE=4 SV=1
MM1 pKa = 7.65VLDD4 pKa = 4.37KK5 pKa = 11.21VSEE8 pKa = 4.73AIAHH12 pKa = 6.58FIGLFQIEE20 pKa = 4.36TEE22 pKa = 4.21QARR25 pKa = 11.84MRR27 pKa = 11.84DD28 pKa = 3.49DD29 pKa = 3.53YY30 pKa = 11.79LQFKK34 pKa = 9.91ALQAAQQPHH43 pKa = 6.6HH44 pKa = 7.42PDD46 pKa = 3.68DD47 pKa = 4.64PSQISFHH54 pKa = 6.55SPYY57 pKa = 11.03DD58 pKa = 3.68FDD60 pKa = 6.46DD61 pKa = 5.08PDD63 pKa = 5.22PGIHH67 pKa = 5.65YY68 pKa = 9.96VPNGPDD74 pKa = 3.48YY75 pKa = 11.18VSPAPVDD82 pKa = 3.19IANYY86 pKa = 7.86PQLEE90 pKa = 4.54VPVPTDD96 pKa = 3.51VQALAYY102 pKa = 8.72PGYY105 pKa = 10.08VYY107 pKa = 10.15PIIHH111 pKa = 6.91AGSHH115 pKa = 4.7SLARR119 pKa = 11.84MFEE122 pKa = 4.22LQPPGAVAAHH132 pKa = 6.77ISQVNALSDD141 pKa = 3.53NDD143 pKa = 3.88FVHH146 pKa = 6.57VGPGNVEE153 pKa = 3.74FHH155 pKa = 7.15QIGTPNLALAGLLDD169 pKa = 3.95QADD172 pKa = 3.67QALPIGPVAFGAAQDD187 pKa = 3.44IGDD190 pKa = 4.22FVSGSAAALNALVAGNEE207 pKa = 4.21GATSDD212 pKa = 5.0SIGAGDD218 pKa = 3.93GDD220 pKa = 4.18GVTIEE225 pKa = 4.75TIVSAFNEE233 pKa = 4.09PAADD237 pKa = 4.27AIYY240 pKa = 10.81VNGHH244 pKa = 5.95IVTTAPRR251 pKa = 11.84LDD253 pKa = 4.24DD254 pKa = 4.17MLPSASPLAKK264 pKa = 10.1DD265 pKa = 4.56APDD268 pKa = 4.28PAPDD272 pKa = 3.69PTPAGGNHH280 pKa = 7.63AINGGGDD287 pKa = 3.32SAAGSSAYY295 pKa = 10.13GQGALSSDD303 pKa = 3.33MSVTLGTGANALSNAASLVNDD324 pKa = 4.37ALAGGVFAVAGNHH337 pKa = 5.97ISLDD341 pKa = 3.9AIVQINAWSDD351 pKa = 3.17SDD353 pKa = 5.1SIGASLAGWNGMSHH367 pKa = 5.58QAATAAFNIAEE378 pKa = 4.42MQRR381 pKa = 11.84IDD383 pKa = 3.12TSTSDD388 pKa = 3.61GAGAAHH394 pKa = 7.2PGFPKK399 pKa = 10.24AWVVTEE405 pKa = 3.76ISGDD409 pKa = 3.84YY410 pKa = 10.98VSLNWVQQLNFVTDD424 pKa = 3.2NDD426 pKa = 4.38TAFVTSSHH434 pKa = 6.08GVTTMVGTGEE444 pKa = 4.01NQTFNNVSVADD455 pKa = 4.07LGHH458 pKa = 6.43YY459 pKa = 9.73YY460 pKa = 10.89DD461 pKa = 5.18LVLIGGNYY469 pKa = 9.29YY470 pKa = 10.37DD471 pKa = 4.93ANIIAQTNVLLDD483 pKa = 4.06DD484 pKa = 5.03DD485 pKa = 4.51VAGSIGGFQTSGTGTVSTSDD505 pKa = 3.2NLLWNDD511 pKa = 3.03ARR513 pKa = 11.84IVTVGSNTVDD523 pKa = 3.33ALPSGFAKK531 pKa = 10.65ALQDD535 pKa = 4.44FEE537 pKa = 6.76GGDD540 pKa = 3.72HH541 pKa = 6.3TLGAAVLNDD550 pKa = 3.73DD551 pKa = 5.02AFQGMAGLRR560 pKa = 11.84VLYY563 pKa = 10.05ISGSIYY569 pKa = 10.28DD570 pKa = 4.02LQYY573 pKa = 10.4IHH575 pKa = 5.79QTNVLGDD582 pKa = 3.64SDD584 pKa = 4.58QIALAMNTTQAGDD597 pKa = 3.47GGDD600 pKa = 3.25WSVTTGSDD608 pKa = 3.41ALINTARR615 pKa = 11.84IVDD618 pKa = 4.34ADD620 pKa = 4.18PAAKK624 pKa = 9.47TYY626 pKa = 11.09VGGDD630 pKa = 3.38HH631 pKa = 6.96YY632 pKa = 11.48SDD634 pKa = 3.96EE635 pKa = 5.16LLVQTDD641 pKa = 4.28IIRR644 pKa = 11.84TDD646 pKa = 3.16HH647 pKa = 6.96LLEE650 pKa = 4.42TRR652 pKa = 11.84DD653 pKa = 3.35GDD655 pKa = 4.01HH656 pKa = 6.81LVNEE660 pKa = 4.69AVAFLSDD667 pKa = 5.35DD668 pKa = 3.91MTHH671 pKa = 7.1HH672 pKa = 7.41DD673 pKa = 3.91AAYY676 pKa = 10.35ANPDD680 pKa = 3.7DD681 pKa = 5.68PISALQAPVDD691 pKa = 3.52IHH693 pKa = 6.51PAHH696 pKa = 7.09ADD698 pKa = 2.76IMQSVISS705 pKa = 3.93

Molecular weight: 73.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V8RTR6|A0A1V8RTR6_9RHIZ Oxidoreductase OS=Pseudaminobacter manganicus OX=1873176 GN=BFN67_14985 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.9GGRR28 pKa = 11.84GVIVARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4404

0

4404

1361994

29

3478

309.3

33.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.326 ± 0.046

0.8 ± 0.011

5.735 ± 0.028

5.749 ± 0.035

3.843 ± 0.025

8.634 ± 0.051

2.072 ± 0.019

5.457 ± 0.023

3.692 ± 0.031

9.919 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.017

2.689 ± 0.021

4.966 ± 0.029

2.989 ± 0.022

6.949 ± 0.04

5.444 ± 0.025

5.23 ± 0.029

7.36 ± 0.027

1.303 ± 0.014

2.265 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski