Cellulosimicrobium cellulans J1
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3743 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X7DMU4|A0A1X7DMU4_CELCE Beta-N-acetylhexosaminidase OS=Cellulosimicrobium cellulans J1 OX=903523 GN=SAMN02744115_01861 PE=3 SV=1
MM1 pKa = 7.51 ALVTAGSGLAAAAAPANAATVYY23 pKa = 9.46 EE24 pKa = 5.04 LEE26 pKa = 4.65 GAWSAGTPTTVATGDD41 pKa = 3.59 AVSASWWFNLNDD53 pKa = 4.1 DD54 pKa = 4.26 AAAPGNAPVDD64 pKa = 3.86 DD65 pKa = 3.84 VTITVTGQGAVFDD78 pKa = 5.2 SIPPVCLTDD87 pKa = 3.93 GVDD90 pKa = 3.61 PASSISDD97 pKa = 3.52 DD98 pKa = 4.13 GATLVCNVGTQDD110 pKa = 3.31 EE111 pKa = 4.83 GTAFALTTPMRR122 pKa = 11.84 VTAQTGEE129 pKa = 4.23 PVSAGATVADD139 pKa = 4.23 QEE141 pKa = 4.48 ATVPDD146 pKa = 4.42 LVVQNAFLQDD156 pKa = 3.58 IRR158 pKa = 11.84 WVQATTQSGNTATYY172 pKa = 10.75 RR173 pKa = 11.84 DD174 pKa = 3.99 MVFNWTLFHH183 pKa = 6.43 GTHH186 pKa = 6.11 SPAGPSEE193 pKa = 3.99 VTYY196 pKa = 11.34 ALTLSNTLGATMQLGPDD213 pKa = 3.79 GCRR216 pKa = 11.84 AFTSGSASGHH226 pKa = 5.77 PWSGGSNDD234 pKa = 4.0 PSQTAPFVGTCTLTRR249 pKa = 11.84 NSSTSYY255 pKa = 11.25 SLTLSGIDD263 pKa = 3.61 YY264 pKa = 10.89 SHH266 pKa = 6.39 TQVPTKK272 pKa = 10.78 DD273 pKa = 3.27 SAGANLPTDD282 pKa = 3.9 RR283 pKa = 11.84 VAVASGQVQIRR294 pKa = 11.84 VMTASNSGGITLTSNAPTYY313 pKa = 9.7 TAVDD317 pKa = 3.87 GQTYY321 pKa = 10.06 TDD323 pKa = 3.74 DD324 pKa = 3.77 ASNNSVNRR332 pKa = 11.84 TWTAGYY338 pKa = 8.59 WSHH341 pKa = 6.38 AWTPAYY347 pKa = 9.11 TGQSGSSWSDD357 pKa = 3.03 TYY359 pKa = 10.95 RR360 pKa = 11.84 VAAGTEE366 pKa = 4.13 VSSFASVNYY375 pKa = 9.91 PNVSTPNATQGGLCVVLDD393 pKa = 3.89 TQNVEE398 pKa = 4.09 FTGATTRR405 pKa = 11.84 FDD407 pKa = 3.85 NNRR410 pKa = 11.84 TGPEE414 pKa = 3.63 AGLPLAYY421 pKa = 9.35 YY422 pKa = 9.26 TGTSALLNPDD432 pKa = 3.39 SGSYY436 pKa = 9.59 QPNDD440 pKa = 3.38 FTCNGTTGWTTTRR453 pKa = 11.84 PTDD456 pKa = 3.42 LSTIKK461 pKa = 10.5 AVRR464 pKa = 11.84 TVFTPTNAIKK474 pKa = 10.33 EE475 pKa = 4.3 RR476 pKa = 11.84 LVALQVTQRR485 pKa = 11.84 VKK487 pKa = 9.62 EE488 pKa = 4.25 TTPVGRR494 pKa = 11.84 DD495 pKa = 2.72 VWTWGAYY502 pKa = 8.71 INAVNTSNVWVHH514 pKa = 6.27 PNRR517 pKa = 11.84 SSDD520 pKa = 3.67 TADD523 pKa = 3.12 MPLNGTATPGSRR535 pKa = 11.84 YY536 pKa = 10.06 AYY538 pKa = 9.86 AAGGRR543 pKa = 11.84 DD544 pKa = 3.69 VLRR547 pKa = 11.84 VIGVTPDD554 pKa = 2.91 VRR556 pKa = 11.84 KK557 pKa = 9.93 SVLPITVDD565 pKa = 3.37 PSGEE569 pKa = 3.76 ATYY572 pKa = 10.49 TLTYY576 pKa = 9.71 SANGTGAVAPTVDD589 pKa = 5.23 DD590 pKa = 3.95 YY591 pKa = 12.31 VLVDD595 pKa = 4.0 TLPAGATYY603 pKa = 10.82 VGGSASPEE611 pKa = 3.86 PAVTTNGSGQQVLTWTLDD629 pKa = 3.51 GVATNAEE636 pKa = 4.18 HH637 pKa = 6.16 SLSYY641 pKa = 10.46 VVRR644 pKa = 11.84 FPDD647 pKa = 4.15 DD648 pKa = 3.49 LQGGDD653 pKa = 3.21 RR654 pKa = 11.84 LVNTVSAAVEE664 pKa = 4.5 GVTSDD669 pKa = 3.81 DD670 pKa = 3.29 ATASVVVSDD679 pKa = 4.58 SGLTKK684 pKa = 10.02 IIKK687 pKa = 8.82 TADD690 pKa = 3.09 QAFIPNLAGDD700 pKa = 4.34 GVGTGSWTVLVRR712 pKa = 11.84 SQDD715 pKa = 3.85 PIAQSFVDD723 pKa = 4.98 VIDD726 pKa = 3.49 ILPYY730 pKa = 10.33 VGDD733 pKa = 3.75 GRR735 pKa = 11.84 GTAFSGSYY743 pKa = 10.68 LLDD746 pKa = 3.98 GPVTSSIGGTVYY758 pKa = 9.49 YY759 pKa = 7.49 TTADD763 pKa = 3.77 PTTLTDD769 pKa = 5.21 DD770 pKa = 4.48 PADD773 pKa = 4.2 PSNGSAGSVAGNTVGWTTEE792 pKa = 3.93 YY793 pKa = 10.77 TPDD796 pKa = 3.1 ATAVRR801 pKa = 11.84 VITGTLAPRR810 pKa = 11.84 GEE812 pKa = 4.36 LTISVPVVTDD822 pKa = 3.21 GMVGGDD828 pKa = 3.36 TLVNRR833 pKa = 11.84 SQGRR837 pKa = 11.84 AEE839 pKa = 3.9 NTRR842 pKa = 11.84 LVMRR846 pKa = 11.84 TSAPTSIANYY856 pKa = 10.02 YY857 pKa = 9.47 SASLKK862 pKa = 10.66 KK863 pKa = 10.44 YY864 pKa = 9.53 VQDD867 pKa = 5.32 ADD869 pKa = 4.43 GEE871 pKa = 4.37 WRR873 pKa = 11.84 DD874 pKa = 3.72 ANDD877 pKa = 3.96 PLDD880 pKa = 3.91 YY881 pKa = 10.67 PQFRR885 pKa = 11.84 VGDD888 pKa = 3.75 TVRR891 pKa = 11.84 YY892 pKa = 9.3 RR893 pKa = 11.84 IVVEE897 pKa = 3.83 NTGQGTLTNVVVSDD911 pKa = 4.21 DD912 pKa = 4.09 LQPEE916 pKa = 4.52 LGSFTIEE923 pKa = 3.76 SLAPGEE929 pKa = 4.43 VEE931 pKa = 3.81 SHH933 pKa = 5.59 EE934 pKa = 4.23 FEE936 pKa = 4.35 IVLSEE941 pKa = 4.11 PVDD944 pKa = 3.67 DD945 pKa = 4.79 AVVNTACADD954 pKa = 3.47 ADD956 pKa = 3.81 VPEE959 pKa = 5.03 DD960 pKa = 3.61 SGVEE964 pKa = 3.82 PTINCDD970 pKa = 3.08 PAGFTVVGTPTHH982 pKa = 5.34 TKK984 pKa = 9.57 EE985 pKa = 4.21 IVSATPVGDD994 pKa = 3.73 GRR996 pKa = 11.84 WEE998 pKa = 3.86 VVYY1001 pKa = 10.24 EE1002 pKa = 4.08 VVVSNTQTPSTTYY1015 pKa = 10.84 SLTDD1019 pKa = 3.4 EE1020 pKa = 5.01 LHH1022 pKa = 6.03 FTDD1025 pKa = 3.54 QATIVSATVTQAPDD1039 pKa = 3.58 GVVLADD1045 pKa = 4.12 PAWDD1049 pKa = 3.67 GQEE1052 pKa = 4.05 NLTIASDD1059 pKa = 3.94 VPLAGNDD1066 pKa = 3.44 DD1067 pKa = 4.44 DD1068 pKa = 6.15 GYY1070 pKa = 9.77 EE1071 pKa = 3.86 PHH1073 pKa = 6.83 VYY1075 pKa = 9.82 RR1076 pKa = 11.84 VTVLADD1082 pKa = 3.21 VPLQLDD1088 pKa = 4.16 GAGSGADD1095 pKa = 4.74 DD1096 pKa = 3.83 PTACPAEE1103 pKa = 4.67 GSDD1106 pKa = 4.92 ADD1108 pKa = 3.46 QGFNNTSGLTGPDD1121 pKa = 3.52 GEE1123 pKa = 4.59 TEE1125 pKa = 4.02 EE1126 pKa = 5.26 DD1127 pKa = 3.73 QACAPIPSIDD1137 pKa = 3.44 VEE1139 pKa = 4.34 KK1140 pKa = 10.55 RR1141 pKa = 11.84 VAAGPTPNGDD1151 pKa = 3.68 GTWTVTYY1158 pKa = 10.57 DD1159 pKa = 3.59 VVATNSGTGDD1169 pKa = 3.25 GVYY1172 pKa = 10.42 EE1173 pKa = 4.15 VTDD1176 pKa = 3.49 RR1177 pKa = 11.84 MTADD1181 pKa = 3.33 GDD1183 pKa = 4.17 LEE1185 pKa = 4.6 VVSGAVTSAPDD1196 pKa = 3.62 GVTPSATWTGLGAEE1210 pKa = 4.51 GAPEE1214 pKa = 3.87 NVIASAVTLPAGTSHH1229 pKa = 7.15 AYY1231 pKa = 8.88 EE1232 pKa = 4.18 VQVVLTIAPGTEE1244 pKa = 3.95 GAPVVTPCSAEE1255 pKa = 3.86 PGGSSGGLSNAAGVEE1270 pKa = 4.35 HH1271 pKa = 7.47 NDD1273 pKa = 3.45 LTDD1276 pKa = 3.28 TAEE1279 pKa = 4.23 ACVTLAAITVEE1290 pKa = 4.4 KK1291 pKa = 10.24 SISAGPTPNGDD1302 pKa = 3.71 GTWTVVYY1309 pKa = 10.37 DD1310 pKa = 4.06 VVAEE1314 pKa = 4.16 NVGAAAGEE1322 pKa = 3.96 YY1323 pKa = 10.18 DD1324 pKa = 3.48 VYY1326 pKa = 11.69 DD1327 pKa = 3.63 RR1328 pKa = 11.84 LRR1330 pKa = 11.84 YY1331 pKa = 9.77 GDD1333 pKa = 4.22 GIEE1336 pKa = 4.2 IVDD1339 pKa = 3.89 AAVTSGPDD1347 pKa = 3.39 GVTTNAAWTGLGEE1360 pKa = 4.78 PDD1362 pKa = 3.09 TAAEE1366 pKa = 4.15 NLVAADD1372 pKa = 3.7 VEE1374 pKa = 4.48 LAAGGLHH1381 pKa = 5.92 TFQVEE1386 pKa = 4.13 VTVRR1390 pKa = 11.84 MDD1392 pKa = 3.22 EE1393 pKa = 4.03 ATIDD1397 pKa = 3.94 PVALRR1402 pKa = 11.84 CPPPGSGASGGLANSTSLDD1421 pKa = 3.39 HH1422 pKa = 6.64 NGIVAEE1428 pKa = 4.69 DD1429 pKa = 4.23 EE1430 pKa = 4.67 VCASLPLIGVEE1441 pKa = 4.39 KK1442 pKa = 10.27 SISAGPVPQGDD1453 pKa = 4.2 GTWAITYY1460 pKa = 10.48 DD1461 pKa = 4.51 LVATNAGEE1469 pKa = 3.96 AAGDD1473 pKa = 3.59 YY1474 pKa = 11.2 DD1475 pKa = 5.31 LVDD1478 pKa = 3.49 RR1479 pKa = 11.84 LQYY1482 pKa = 10.91 GEE1484 pKa = 4.3 GVVVEE1489 pKa = 4.48 SAAVVSAPDD1498 pKa = 3.83 GVATEE1503 pKa = 4.8 AGWTGQGAAGSTEE1516 pKa = 3.95 NVVAAGVTLGAGAAHH1531 pKa = 7.38 TYY1533 pKa = 8.7 QVQVVVSLDD1542 pKa = 3.48 RR1543 pKa = 11.84 AVVTPSTLACPEE1555 pKa = 4.54 PGSGEE1560 pKa = 4.16 GGGLANATEE1569 pKa = 4.29 LTHH1572 pKa = 7.49 NGEE1575 pKa = 4.31 TQGDD1579 pKa = 4.21 DD1580 pKa = 3.41 VCAEE1584 pKa = 4.43 LPLIDD1589 pKa = 3.88 IAKK1592 pKa = 8.81 SLSGAVTPVEE1602 pKa = 4.32 GEE1604 pKa = 3.9 PGVYY1608 pKa = 10.07 DD1609 pKa = 3.68 AVYY1612 pKa = 10.34 EE1613 pKa = 4.17 ITVTNAGPGDD1623 pKa = 3.97 GLYY1626 pKa = 11.08 DD1627 pKa = 3.93 LTDD1630 pKa = 3.39 EE1631 pKa = 4.6 LAPGEE1636 pKa = 4.23 GVEE1639 pKa = 4.15 VLGVQDD1645 pKa = 4.0 VVTDD1649 pKa = 3.75 APEE1652 pKa = 3.94 PVGLNPGFDD1661 pKa = 3.71 GTSDD1665 pKa = 3.6 TLVVAGQPIAGATTAPVVHH1684 pKa = 7.1 TYY1686 pKa = 9.23 TVTVRR1691 pKa = 11.84 YY1692 pKa = 9.04 AADD1695 pKa = 3.72 LSDD1698 pKa = 4.69 VEE1700 pKa = 5.49 IPDD1703 pKa = 3.63 TDD1705 pKa = 3.28 ACTTGDD1711 pKa = 3.45 GSTVAGGLANTATVGWNGLDD1731 pKa = 3.76 GTDD1734 pKa = 4.14 DD1735 pKa = 3.54 ACVRR1739 pKa = 11.84 PGKK1742 pKa = 8.38 PTLDD1746 pKa = 3.44 KK1747 pKa = 11.02 ALVSAAPAGDD1757 pKa = 3.84 GTWEE1761 pKa = 4.04 VVYY1764 pKa = 10.66 DD1765 pKa = 3.7 LTVGNVGGEE1774 pKa = 3.86 ATTYY1778 pKa = 11.33 DD1779 pKa = 4.64 LDD1781 pKa = 5.91 DD1782 pKa = 4.55 EE1783 pKa = 5.49 LLFAPVVTVDD1793 pKa = 3.56 SVAVTGPEE1801 pKa = 3.91 GVTVNAGFDD1810 pKa = 3.34 GATDD1814 pKa = 3.43 QRR1816 pKa = 11.84 IATGVSIAGMDD1827 pKa = 3.73 DD1828 pKa = 3.11 EE1829 pKa = 6.21 GYY1831 pKa = 10.42 APHH1834 pKa = 7.05 VYY1836 pKa = 9.69 RR1837 pKa = 11.84 VTVVAHH1843 pKa = 5.84 VPLSFAADD1851 pKa = 3.73 DD1852 pKa = 3.8 VDD1854 pKa = 4.83 GDD1856 pKa = 4.33 GTGSPACTVPAGSNTLQQGLNNAATLTDD1884 pKa = 3.88 EE1885 pKa = 4.55 TGGTQTDD1892 pKa = 3.89 TDD1894 pKa = 4.43 CAPLPSIDD1902 pKa = 3.66 VTKK1905 pKa = 10.33 STDD1908 pKa = 3.27 GDD1910 pKa = 4.0 PVQGDD1915 pKa = 3.61 DD1916 pKa = 4.03 GSWTVRR1922 pKa = 11.84 YY1923 pKa = 9.43 AVTVTNDD1930 pKa = 2.98 GGSAGAYY1937 pKa = 10.1 DD1938 pKa = 3.59 LTDD1941 pKa = 3.5 RR1942 pKa = 11.84 LRR1944 pKa = 11.84 YY1945 pKa = 8.97 GAGITVRR1952 pKa = 11.84 SATVTATPEE1961 pKa = 4.09 GVTASATWTGQGAEE1975 pKa = 3.8 GDD1977 pKa = 3.81 AANVVAADD1985 pKa = 3.42 VDD1987 pKa = 4.35 LAPGATHH1994 pKa = 6.66 VYY1996 pKa = 9.41 RR1997 pKa = 11.84 VEE1999 pKa = 3.96 VVAAVDD2005 pKa = 3.76 PGRR2008 pKa = 11.84 ADD2010 pKa = 3.07 ATTFTCPAPGSGEE2023 pKa = 3.81 AGGFANTAGVGHH2035 pKa = 7.01 NDD2037 pKa = 3.09 LTDD2040 pKa = 3.84 AADD2043 pKa = 3.48 ACAAPPEE2050 pKa = 4.38 PANPGTPGKK2059 pKa = 9.67 PGGSLATTGATLAWVAGGAALLLLLGMLALLAARR2093 pKa = 11.84 RR2094 pKa = 11.84 RR2095 pKa = 11.84 RR2096 pKa = 11.84 LTSS2099 pKa = 2.99
Molecular weight: 213.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.757
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.935
Patrickios 1.354
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A1X7CNR9|A0A1X7CNR9_CELCE Inositol-1-monophosphatase OS=Cellulosimicrobium cellulans J1 OX=903523 GN=SAMN02744115_00806 PE=3 SV=1
MM1 pKa = 7.63 AGLRR5 pKa = 11.84 PAVEE9 pKa = 4.22 PVAVPARR16 pKa = 11.84 AGARR20 pKa = 11.84 RR21 pKa = 11.84 VPTPVAPATVVARR34 pKa = 11.84 PARR37 pKa = 11.84 STTSVRR43 pKa = 11.84 RR44 pKa = 11.84 VPVEE48 pKa = 3.84 PPAAALPRR56 pKa = 11.84 ARR58 pKa = 11.84 GPLRR62 pKa = 11.84 VAPLTSVAVALARR75 pKa = 11.84 PPVVPTRR82 pKa = 11.84 GTVVTARR89 pKa = 11.84 GTVAATRR96 pKa = 11.84 TLVATGVPAAVLAARR111 pKa = 11.84 AVGTVLAARR120 pKa = 11.84 TAPGSIPTGAVTSVAGAVTTVRR142 pKa = 11.84 VRR144 pKa = 11.84 ATAVGSTVVATVALRR159 pKa = 11.84 TAALGTVRR167 pKa = 11.84 TRR169 pKa = 11.84 ASTVGSVPVAALRR182 pKa = 11.84 APAPVARR189 pKa = 11.84 AALVTAAATVTRR201 pKa = 11.84 RR202 pKa = 11.84 VTAGRR207 pKa = 11.84 PAMSPATLVAGASSTVLVASTAAVVARR234 pKa = 11.84 PRR236 pKa = 11.84 TVRR239 pKa = 11.84 LGLVGAVVHH248 pKa = 6.23 EE249 pKa = 4.92 GSLSFWSLRR258 pKa = 11.84 RR259 pKa = 11.84 PSSHH263 pKa = 6.85 ASVLGHH269 pKa = 6.53 AAGRR273 pKa = 11.84 LL274 pKa = 3.55
Molecular weight: 26.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.488
IPC2_protein 11.082
IPC_protein 12.705
Toseland 12.866
ProMoST 13.364
Dawson 12.866
Bjellqvist 12.866
Wikipedia 13.334
Rodwell 12.354
Grimsley 12.896
Solomon 13.364
Lehninger 13.261
Nozaki 12.866
DTASelect 12.866
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.076
IPC_peptide 13.364
IPC2_peptide 12.354
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3743
0
3743
1281283
27
2099
342.3
36.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.844 ± 0.06
0.512 ± 0.01
6.72 ± 0.036
5.42 ± 0.03
2.516 ± 0.023
9.564 ± 0.036
2.02 ± 0.019
2.536 ± 0.026
1.343 ± 0.023
9.969 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.406 ± 0.014
1.473 ± 0.022
6.25 ± 0.035
2.433 ± 0.021
7.985 ± 0.05
4.761 ± 0.023
6.398 ± 0.043
10.445 ± 0.041
1.544 ± 0.016
1.861 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here