Paenibacillus paeoniae
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5044 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A371P7L8|A0A371P7L8_9BACL Glycoside hydrolase family 16 protein OS=Paenibacillus paeoniae OX=2292705 GN=DX130_19750 PE=4 SV=1
MM1 pKa = 7.31 RR2 pKa = 11.84 HH3 pKa = 5.94 IVTVWGAHH11 pKa = 5.5 FSSEE15 pKa = 4.17 DD16 pKa = 3.32 EE17 pKa = 3.87 LAAFVEE23 pKa = 4.42 TLYY26 pKa = 11.28 DD27 pKa = 3.74 EE28 pKa = 6.26 DD29 pKa = 5.76 GDD31 pKa = 4.42 ALLSDD36 pKa = 4.93 FEE38 pKa = 5.17 DD39 pKa = 4.32 SIGSDD44 pKa = 3.94 GIDD47 pKa = 3.22 EE48 pKa = 4.88 DD49 pKa = 4.51 FMEE52 pKa = 4.3 IHH54 pKa = 6.7 HH55 pKa = 6.88 LNDD58 pKa = 3.43 EE59 pKa = 4.32 EE60 pKa = 4.11 EE61 pKa = 4.08 QLAFIQYY68 pKa = 9.81 LKK70 pKa = 10.72 SEE72 pKa = 4.36 YY73 pKa = 10.78 VPDD76 pKa = 4.2 DD77 pKa = 3.66 SFVNGLPATINEE89 pKa = 4.43 SIKK92 pKa = 10.19 PYY94 pKa = 10.81 NSIILVSGSDD104 pKa = 3.47 SPYY107 pKa = 10.42 GSINEE112 pKa = 4.29 EE113 pKa = 3.67 LFQFDD118 pKa = 3.77 KK119 pKa = 11.51 ADD121 pKa = 3.71 VEE123 pKa = 4.52 SDD125 pKa = 3.81 SPVVLLTSVEE135 pKa = 4.25 YY136 pKa = 10.38 EE137 pKa = 4.16 GRR139 pKa = 3.6
Molecular weight: 15.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.49
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.401
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 3.986
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.808
Patrickios 1.837
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A371P7U4|A0A371P7U4_9BACL 30S ribosomal protein S6 OS=Paenibacillus paeoniae OX=2292705 GN=rpsF PE=3 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.84 PNVSKK11 pKa = 10.73 RR12 pKa = 11.84 AKK14 pKa = 8.72 VHH16 pKa = 5.43 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.78 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.84 VLSAA44 pKa = 4.11
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5044
0
5044
1661949
26
4634
329.5
36.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.314 ± 0.037
0.693 ± 0.009
5.142 ± 0.026
6.81 ± 0.043
4.005 ± 0.025
7.481 ± 0.052
2.147 ± 0.02
6.81 ± 0.032
5.15 ± 0.029
10.234 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.886 ± 0.02
3.748 ± 0.029
3.895 ± 0.021
3.789 ± 0.022
5.124 ± 0.034
6.591 ± 0.029
5.446 ± 0.053
6.933 ± 0.028
1.281 ± 0.015
3.521 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here