Streptococcus phage Javan576
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6BAQ0|A0A4D6BAQ0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan576 OX=2548268 GN=Javan576_0017 PE=4 SV=1
MM1 pKa = 7.17 QFDD4 pKa = 3.89 EE5 pKa = 5.04 VIEE8 pKa = 4.64 FNDD11 pKa = 3.32 EE12 pKa = 4.16 LYY14 pKa = 10.82 QKK16 pKa = 10.81 NIADD20 pKa = 3.86 HH21 pKa = 6.71 EE22 pKa = 4.59 FPKK25 pKa = 10.71 TDD27 pKa = 4.09 DD28 pKa = 3.89 LDD30 pKa = 4.46 GKK32 pKa = 10.35 GGEE35 pKa = 4.44 YY36 pKa = 10.18 EE37 pKa = 3.99
Molecular weight: 4.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.207
IPC2_protein 3.948
IPC_protein 3.834
Toseland 3.643
ProMoST 3.935
Dawson 3.821
Bjellqvist 4.062
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.948
Patrickios 3.465
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A4D6BDT5|A0A4D6BDT5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan576 OX=2548268 GN=Javan576_0028 PE=4 SV=1
MM1 pKa = 7.34 SRR3 pKa = 11.84 LIGADD8 pKa = 3.36 RR9 pKa = 11.84 LISKK13 pKa = 9.11 LKK15 pKa = 10.03 RR16 pKa = 11.84 LSSQRR21 pKa = 11.84 QTEE24 pKa = 4.01 IMAKK28 pKa = 9.84 AVHH31 pKa = 5.97 NAAKK35 pKa = 10.53 NVVQADD41 pKa = 3.5 AKK43 pKa = 10.81 LRR45 pKa = 11.84 APANNGDD52 pKa = 3.56 LRR54 pKa = 11.84 AGIKK58 pKa = 10.31 VRR60 pKa = 11.84 MSKK63 pKa = 10.21 SGNPRR68 pKa = 11.84 AEE70 pKa = 4.01 VVSTSDD76 pKa = 2.51 HH77 pKa = 6.03 GGFVEE82 pKa = 6.18 FGTGPKK88 pKa = 10.05 GAANHH93 pKa = 6.59 AGISPNVSVSYY104 pKa = 10.67 RR105 pKa = 11.84 STPWYY110 pKa = 9.47 VHH112 pKa = 6.4 EE113 pKa = 4.39 SQIDD117 pKa = 3.44 VGPYY121 pKa = 9.34 RR122 pKa = 11.84 FQKK125 pKa = 10.66 LGEE128 pKa = 4.1 FYY130 pKa = 11.56 KK131 pKa = 10.51 MFGQVAQPYY140 pKa = 9.58 LYY142 pKa = 9.66 PALKK146 pKa = 10.66 DD147 pKa = 3.27 NEE149 pKa = 4.03 EE150 pKa = 3.97 RR151 pKa = 11.84 VTKK154 pKa = 10.58 NINRR158 pKa = 11.84 FVKK161 pKa = 10.1 RR162 pKa = 11.84 KK163 pKa = 9.94 LIEE166 pKa = 4.24 EE167 pKa = 4.15 VSKK170 pKa = 11.39
Molecular weight: 18.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.176
IPC2_protein 9.516
IPC_protein 9.619
Toseland 10.35
ProMoST 9.97
Dawson 10.482
Bjellqvist 10.116
Wikipedia 10.628
Rodwell 10.994
Grimsley 10.54
Solomon 10.511
Lehninger 10.496
Nozaki 10.306
DTASelect 10.116
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.394
Patrickios 10.701
IPC_peptide 10.526
IPC2_peptide 8.595
IPC2.peptide.svr19 8.649
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
13081
37
1520
195.2
22.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.438 ± 0.327
0.413 ± 0.073
6.467 ± 0.239
7.492 ± 0.514
3.983 ± 0.214
6.284 ± 0.414
1.521 ± 0.212
6.98 ± 0.268
7.782 ± 0.374
8.677 ± 0.4
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.231
5.252 ± 0.208
2.829 ± 0.251
4.342 ± 0.292
4.564 ± 0.211
5.841 ± 0.394
5.94 ± 0.477
6.766 ± 0.265
1.185 ± 0.109
3.807 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here