Novosphingobium sp. AAP93

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3654 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0K7A1|A0A0N0K7A1_9SPHN Peptidase M16 OS=Novosphingobium sp. AAP93 OX=1523427 GN=IP83_10975 PE=3 SV=1
MM1 pKa = 7.64ANLSTTLTFDD11 pKa = 3.9SFDD14 pKa = 3.67LAEE17 pKa = 4.75GGEE20 pKa = 4.19SRR22 pKa = 11.84LANGYY27 pKa = 10.62GGFNWQQAGIYY38 pKa = 8.99NPNGAIAGYY47 pKa = 9.44VASSGQNILFIAEE60 pKa = 3.9AANNEE65 pKa = 4.05VAGYY69 pKa = 9.52EE70 pKa = 3.97DD71 pKa = 3.96AAAGSALVMSRR82 pKa = 11.84EE83 pKa = 4.22APFTLDD89 pKa = 3.93TIQLSSAFRR98 pKa = 11.84DD99 pKa = 3.62GLTVTIRR106 pKa = 11.84AYY108 pKa = 10.61ADD110 pKa = 3.13QAGTILIGEE119 pKa = 4.44KK120 pKa = 9.71TVTVDD125 pKa = 3.23TAAQSLVSFADD136 pKa = 3.71DD137 pKa = 3.68GLDD140 pKa = 3.16YY141 pKa = 9.88GTFSGATRR149 pKa = 11.84IEE151 pKa = 4.37FNANDD156 pKa = 3.76NNAGTNDD163 pKa = 3.43YY164 pKa = 11.08FGIDD168 pKa = 3.19NLTYY172 pKa = 10.51HH173 pKa = 6.72DD174 pKa = 4.45TPPTFTLDD182 pKa = 3.32FDD184 pKa = 6.18DD185 pKa = 4.49IALAPGAEE193 pKa = 4.31TALGTYY199 pKa = 9.8QGFTFAEE206 pKa = 4.21AGAYY210 pKa = 9.44HH211 pKa = 6.76VDD213 pKa = 3.54GSLPGYY219 pKa = 7.74TAASGTNIGFIAEE232 pKa = 4.17ANNNEE237 pKa = 4.06VVGYY241 pKa = 9.01EE242 pKa = 4.15GQAAGSPVVITNPDD256 pKa = 3.03AFDD259 pKa = 3.76FLGGAFSAAFRR270 pKa = 11.84DD271 pKa = 4.6GVAVTIRR278 pKa = 11.84GYY280 pKa = 11.21SDD282 pKa = 4.43ADD284 pKa = 3.5GFNLVAEE291 pKa = 4.31EE292 pKa = 4.51TIVVRR297 pKa = 11.84AGSAQPFSFEE307 pKa = 4.34TFDD310 pKa = 3.45GLHH313 pKa = 6.14RR314 pKa = 11.84LEE316 pKa = 5.27FSTNDD321 pKa = 3.3GDD323 pKa = 4.95PNTNDD328 pKa = 3.11YY329 pKa = 11.69VGFDD333 pKa = 3.39NLLFRR338 pKa = 11.84AADD341 pKa = 3.42VAAQGEE347 pKa = 4.91GILII351 pKa = 4.17

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1BNJ1|A0A0N1BNJ1_9SPHN Uncharacterized protein OS=Novosphingobium sp. AAP93 OX=1523427 GN=IP83_16315 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.11VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3654

0

3654

1213131

41

6642

332.0

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.772 ± 0.067

0.825 ± 0.012

5.67 ± 0.052

5.435 ± 0.036

3.556 ± 0.025

9.132 ± 0.089

2.044 ± 0.024

4.779 ± 0.023

3.04 ± 0.03

9.924 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.026

2.522 ± 0.034

5.47 ± 0.043

3.046 ± 0.023

6.956 ± 0.047

5.023 ± 0.039

5.36 ± 0.041

7.252 ± 0.029

1.486 ± 0.018

2.258 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski