Amino acid dipepetide frequency for Novosphingobium sp. AAP93

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.113AlaAla: 21.113 ± 0.217
1.055AlaCys: 1.055 ± 0.033
7.763AlaAsp: 7.763 ± 0.128
8.462AlaGlu: 8.462 ± 0.101
4.494AlaPhe: 4.494 ± 0.069
12.056AlaGly: 12.056 ± 0.2
2.554AlaHis: 2.554 ± 0.048
6.402AlaIle: 6.402 ± 0.071
4.354AlaLys: 4.354 ± 0.074
14.69AlaLeu: 14.69 ± 0.199
4.134AlaMet: 4.134 ± 0.08
3.205AlaAsn: 3.205 ± 0.052
6.773AlaPro: 6.773 ± 0.098
4.504AlaGln: 4.504 ± 0.072
9.942AlaArg: 9.942 ± 0.13
6.506AlaSer: 6.506 ± 0.091
6.164AlaThr: 6.164 ± 0.08
9.078AlaVal: 9.078 ± 0.105
1.767AlaTrp: 1.767 ± 0.042
2.709AlaTyr: 2.709 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.067CysAla: 1.067 ± 0.038
0.092CysCys: 0.092 ± 0.009
0.543CysAsp: 0.543 ± 0.021
0.399CysGlu: 0.399 ± 0.017
0.288CysPhe: 0.288 ± 0.016
0.932CysGly: 0.932 ± 0.027
0.251CysHis: 0.251 ± 0.019
0.342CysIle: 0.342 ± 0.019
0.197CysLys: 0.197 ± 0.013
0.729CysLeu: 0.729 ± 0.025
0.199CysMet: 0.199 ± 0.014
0.237CysAsn: 0.237 ± 0.016
0.521CysPro: 0.521 ± 0.022
0.207CysGln: 0.207 ± 0.013
0.525CysArg: 0.525 ± 0.022
0.424CysSer: 0.424 ± 0.02
0.442CysThr: 0.442 ± 0.02
0.554CysVal: 0.554 ± 0.022
0.11CysTrp: 0.11 ± 0.009
0.195CysTyr: 0.195 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.403AspAla: 7.403 ± 0.09
0.51AspCys: 0.51 ± 0.022
2.853AspAsp: 2.853 ± 0.092
3.135AspGlu: 3.135 ± 0.061
2.174AspPhe: 2.174 ± 0.044
5.309AspGly: 5.309 ± 0.121
1.219AspHis: 1.219 ± 0.032
2.567AspIle: 2.567 ± 0.055
1.8AspLys: 1.8 ± 0.042
5.603AspLeu: 5.603 ± 0.076
1.314AspMet: 1.314 ± 0.029
1.325AspAsn: 1.325 ± 0.035
3.939AspPro: 3.939 ± 0.059
1.586AspGln: 1.586 ± 0.041
4.084AspArg: 4.084 ± 0.066
2.192AspSer: 2.192 ± 0.047
2.794AspThr: 2.794 ± 0.165
4.158AspVal: 4.158 ± 0.088
1.098AspTrp: 1.098 ± 0.03
1.64AspTyr: 1.64 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
8.434GluAla: 8.434 ± 0.108
0.342GluCys: 0.342 ± 0.02
2.78GluAsp: 2.78 ± 0.056
2.846GluGlu: 2.846 ± 0.061
1.569GluPhe: 1.569 ± 0.037
4.751GluGly: 4.751 ± 0.079
1.16GluHis: 1.16 ± 0.031
2.757GluIle: 2.757 ± 0.059
1.804GluLys: 1.804 ± 0.049
4.957GluLeu: 4.957 ± 0.069
1.368GluMet: 1.368 ± 0.04
1.296GluAsn: 1.296 ± 0.033
2.62GluPro: 2.62 ± 0.053
1.987GluGln: 1.987 ± 0.043
4.639GluArg: 4.639 ± 0.073
2.082GluSer: 2.082 ± 0.046
3.208GluThr: 3.208 ± 0.055
3.855GluVal: 3.855 ± 0.057
0.889GluTrp: 0.889 ± 0.03
1.0GluTyr: 1.0 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
5.031PheAla: 5.031 ± 0.076
0.335PheCys: 0.335 ± 0.015
2.592PheAsp: 2.592 ± 0.058
1.871PheGlu: 1.871 ± 0.043
1.232PhePhe: 1.232 ± 0.037
3.729PheGly: 3.729 ± 0.059
0.792PheHis: 0.792 ± 0.025
1.43PheIle: 1.43 ± 0.032
0.946PheLys: 0.946 ± 0.029
3.099PheLeu: 3.099 ± 0.059
0.749PheMet: 0.749 ± 0.026
1.093PheAsn: 1.093 ± 0.037
1.651PhePro: 1.651 ± 0.04
0.885PheGln: 0.885 ± 0.025
2.057PheArg: 2.057 ± 0.042
1.917PheSer: 1.917 ± 0.044
2.142PheThr: 2.142 ± 0.046
2.571PheVal: 2.571 ± 0.049
0.514PheTrp: 0.514 ± 0.019
0.928PheTyr: 0.928 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
10.742GlyAla: 10.742 ± 0.183
0.839GlyCys: 0.839 ± 0.029
4.786GlyAsp: 4.786 ± 0.176
4.99GlyGlu: 4.99 ± 0.071
3.731GlyPhe: 3.731 ± 0.066
8.704GlyGly: 8.704 ± 0.299
1.915GlyHis: 1.915 ± 0.048
4.404GlyIle: 4.404 ± 0.068
3.617GlyLys: 3.617 ± 0.061
8.977GlyLeu: 8.977 ± 0.099
2.464GlyMet: 2.464 ± 0.052
2.455GlyAsn: 2.455 ± 0.083
3.985GlyPro: 3.985 ± 0.06
2.97GlyGln: 2.97 ± 0.044
6.003GlyArg: 6.003 ± 0.084
5.051GlySer: 5.051 ± 0.17
5.288GlyThr: 5.288 ± 0.11
6.332GlyVal: 6.332 ± 0.067
1.66GlyTrp: 1.66 ± 0.043
2.405GlyTyr: 2.405 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.623HisAla: 2.623 ± 0.055
0.258HisCys: 0.258 ± 0.014
1.157HisAsp: 1.157 ± 0.031
1.077HisGlu: 1.077 ± 0.032
0.896HisPhe: 0.896 ± 0.027
2.025HisGly: 2.025 ± 0.043
0.558HisHis: 0.558 ± 0.028
0.876HisIle: 0.876 ± 0.026
0.523HisLys: 0.523 ± 0.02
1.88HisLeu: 1.88 ± 0.045
0.495HisMet: 0.495 ± 0.02
0.446HisAsn: 0.446 ± 0.021
1.426HisPro: 1.426 ± 0.036
0.523HisGln: 0.523 ± 0.024
1.401HisArg: 1.401 ± 0.036
0.897HisSer: 0.897 ± 0.025
0.832HisThr: 0.832 ± 0.026
1.572HisVal: 1.572 ± 0.041
0.363HisTrp: 0.363 ± 0.017
0.617HisTyr: 0.617 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
7.527IleAla: 7.527 ± 0.08
0.443IleCys: 0.443 ± 0.02
3.706IleAsp: 3.706 ± 0.063
3.4IleGlu: 3.4 ± 0.059
1.282IlePhe: 1.282 ± 0.032
4.946IleGly: 4.946 ± 0.082
0.89IleHis: 0.89 ± 0.028
1.772IleIle: 1.772 ± 0.045
1.332IleLys: 1.332 ± 0.037
3.474IleLeu: 3.474 ± 0.057
0.896IleMet: 0.896 ± 0.027
1.38IleAsn: 1.38 ± 0.037
2.275IlePro: 2.275 ± 0.046
1.061IleGln: 1.061 ± 0.032
2.904IleArg: 2.904 ± 0.055
2.488IleSer: 2.488 ± 0.045
2.736IleThr: 2.736 ± 0.054
3.721IleVal: 3.721 ± 0.062
0.589IleTrp: 0.589 ± 0.022
0.967IleTyr: 0.967 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
4.436LysAla: 4.436 ± 0.081
0.177LysCys: 0.177 ± 0.012
1.546LysAsp: 1.546 ± 0.035
1.214LysGlu: 1.214 ± 0.035
0.911LysPhe: 0.911 ± 0.032
2.78LysGly: 2.78 ± 0.057
0.532LysHis: 0.532 ± 0.022
1.435LysIle: 1.435 ± 0.042
0.917LysLys: 0.917 ± 0.033
3.324LysLeu: 3.324 ± 0.058
0.686LysMet: 0.686 ± 0.029
0.703LysAsn: 0.703 ± 0.027
2.042LysPro: 2.042 ± 0.041
0.911LysGln: 0.911 ± 0.028
2.228LysArg: 2.228 ± 0.047
1.455LysSer: 1.455 ± 0.036
1.645LysThr: 1.645 ± 0.043
2.467LysVal: 2.467 ± 0.051
0.403LysTrp: 0.403 ± 0.02
0.585LysTyr: 0.585 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
15.354LeuAla: 15.354 ± 0.171
0.764LeuCys: 0.764 ± 0.026
5.882LeuAsp: 5.882 ± 0.081
5.04LeuGlu: 5.04 ± 0.08
3.322LeuPhe: 3.322 ± 0.06
8.879LeuGly: 8.879 ± 0.088
1.945LeuHis: 1.945 ± 0.046
4.609LeuIle: 4.609 ± 0.067
2.991LeuLys: 2.991 ± 0.055
9.323LeuLeu: 9.323 ± 0.123
2.152LeuMet: 2.152 ± 0.046
2.315LeuAsn: 2.315 ± 0.053
5.769LeuPro: 5.769 ± 0.08
2.559LeuGln: 2.559 ± 0.056
6.703LeuArg: 6.703 ± 0.099
5.486LeuSer: 5.486 ± 0.075
5.641LeuThr: 5.641 ± 0.074
7.339LeuVal: 7.339 ± 0.098
1.252LeuTrp: 1.252 ± 0.035
1.919LeuTyr: 1.919 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
3.574MetAla: 3.574 ± 0.059
0.172MetCys: 0.172 ± 0.012
1.15MetAsp: 1.15 ± 0.031
1.021MetGlu: 1.021 ± 0.033
0.655MetPhe: 0.655 ± 0.026
2.006MetGly: 2.006 ± 0.041
0.443MetHis: 0.443 ± 0.02
1.331MetIle: 1.331 ± 0.035
0.824MetLys: 0.824 ± 0.026
2.641MetLeu: 2.641 ± 0.049
0.644MetMet: 0.644 ± 0.027
0.622MetAsn: 0.622 ± 0.024
1.476MetPro: 1.476 ± 0.039
0.745MetGln: 0.745 ± 0.028
1.779MetArg: 1.779 ± 0.038
1.387MetSer: 1.387 ± 0.03
1.735MetThr: 1.735 ± 0.041
1.777MetVal: 1.777 ± 0.041
0.221MetTrp: 0.221 ± 0.014
0.283MetTyr: 0.283 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.417AsnAla: 3.417 ± 0.062
0.24AsnCys: 0.24 ± 0.014
1.354AsnAsp: 1.354 ± 0.068
1.067AsnGlu: 1.067 ± 0.03
0.938AsnPhe: 0.938 ± 0.033
2.442AsnGly: 2.442 ± 0.059
0.477AsnHis: 0.477 ± 0.019
1.128AsnIle: 1.128 ± 0.035
0.619AsnLys: 0.619 ± 0.021
2.506AsnLeu: 2.506 ± 0.054
0.525AsnMet: 0.525 ± 0.021
0.71AsnAsn: 0.71 ± 0.032
1.905AsnPro: 1.905 ± 0.04
0.714AsnGln: 0.714 ± 0.027
1.734AsnArg: 1.734 ± 0.042
1.121AsnSer: 1.121 ± 0.034
1.31AsnThr: 1.31 ± 0.037
1.887AsnVal: 1.887 ± 0.049
0.439AsnTrp: 0.439 ± 0.019
0.692AsnTyr: 0.692 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
7.671ProAla: 7.671 ± 0.108
0.361ProCys: 0.361 ± 0.016
3.663ProAsp: 3.663 ± 0.058
3.796ProGlu: 3.796 ± 0.063
2.001ProPhe: 2.001 ± 0.042
5.17ProGly: 5.17 ± 0.065
1.094ProHis: 1.094 ± 0.032
2.313ProIle: 2.313 ± 0.045
1.549ProLys: 1.549 ± 0.038
5.296ProLeu: 5.296 ± 0.084
1.269ProMet: 1.269 ± 0.035
1.265ProAsn: 1.265 ± 0.034
2.889ProPro: 2.889 ± 0.074
1.808ProGln: 1.808 ± 0.041
3.058ProArg: 3.058 ± 0.061
2.614ProSer: 2.614 ± 0.061
2.557ProThr: 2.557 ± 0.055
4.424ProVal: 4.424 ± 0.067
0.758ProTrp: 0.758 ± 0.026
1.142ProTyr: 1.142 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.016GlnAla: 4.016 ± 0.064
0.237GlnCys: 0.237 ± 0.016
1.376GlnAsp: 1.376 ± 0.031
1.155GlnGlu: 1.155 ± 0.031
1.18GlnPhe: 1.18 ± 0.034
2.53GlnGly: 2.53 ± 0.047
0.644GlnHis: 0.644 ± 0.024
1.789GlnIle: 1.789 ± 0.042
0.87GlnLys: 0.87 ± 0.03
3.104GlnLeu: 3.104 ± 0.051
0.799GlnMet: 0.799 ± 0.027
0.73GlnAsn: 0.73 ± 0.031
1.801GlnPro: 1.801 ± 0.041
1.131GlnGln: 1.131 ± 0.037
2.371GlnArg: 2.371 ± 0.048
1.61GlnSer: 1.61 ± 0.041
1.702GlnThr: 1.702 ± 0.043
2.257GlnVal: 2.257 ± 0.04
0.52GlnTrp: 0.52 ± 0.019
0.639GlnTyr: 0.639 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
8.517ArgAla: 8.517 ± 0.116
0.558ArgCys: 0.558 ± 0.02
3.699ArgAsp: 3.699 ± 0.064
4.063ArgGlu: 4.063 ± 0.07
2.93ArgPhe: 2.93 ± 0.053
5.139ArgGly: 5.139 ± 0.07
1.625ArgHis: 1.625 ± 0.044
3.932ArgIle: 3.932 ± 0.061
2.154ArgLys: 2.154 ± 0.049
7.679ArgLeu: 7.679 ± 0.105
1.96ArgMet: 1.96 ± 0.043
1.677ArgAsn: 1.677 ± 0.041
3.512ArgPro: 3.512 ± 0.062
2.343ArgGln: 2.343 ± 0.055
5.165ArgArg: 5.165 ± 0.095
3.435ArgSer: 3.435 ± 0.065
3.458ArgThr: 3.458 ± 0.058
4.722ArgVal: 4.722 ± 0.052
1.149ArgTrp: 1.149 ± 0.032
1.842ArgTyr: 1.842 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
6.617SerAla: 6.617 ± 0.129
0.41SerCys: 0.41 ± 0.019
2.841SerAsp: 2.841 ± 0.051
2.572SerGlu: 2.572 ± 0.044
2.016SerPhe: 2.016 ± 0.037
5.476SerGly: 5.476 ± 0.13
0.978SerHis: 0.978 ± 0.028
2.373SerIle: 2.373 ± 0.049
1.345SerLys: 1.345 ± 0.036
4.994SerLeu: 4.994 ± 0.068
1.079SerMet: 1.079 ± 0.033
1.309SerAsn: 1.309 ± 0.04
2.782SerPro: 2.782 ± 0.051
1.478SerGln: 1.478 ± 0.035
3.259SerArg: 3.259 ± 0.053
2.569SerSer: 2.569 ± 0.057
2.456SerThr: 2.456 ± 0.049
3.52SerVal: 3.52 ± 0.059
0.787SerTrp: 0.787 ± 0.024
1.368SerTyr: 1.368 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
6.914ThrAla: 6.914 ± 0.082
0.462ThrCys: 0.462 ± 0.023
2.738ThrAsp: 2.738 ± 0.061
2.371ThrGlu: 2.371 ± 0.051
1.975ThrPhe: 1.975 ± 0.045
5.678ThrGly: 5.678 ± 0.115
0.979ThrHis: 0.979 ± 0.03
2.746ThrIle: 2.746 ± 0.053
1.34ThrLys: 1.34 ± 0.037
5.728ThrLeu: 5.728 ± 0.115
1.185ThrMet: 1.185 ± 0.029
1.352ThrAsn: 1.352 ± 0.037
3.282ThrPro: 3.282 ± 0.06
1.468ThrGln: 1.468 ± 0.043
3.454ThrArg: 3.454 ± 0.057
2.788ThrSer: 2.788 ± 0.059
2.908ThrThr: 2.908 ± 0.063
4.23ThrVal: 4.23 ± 0.079
0.738ThrTrp: 0.738 ± 0.025
1.264ThrTyr: 1.264 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
9.1ValAla: 9.1 ± 0.102
0.637ValCys: 0.637 ± 0.022
3.984ValAsp: 3.984 ± 0.065
4.089ValGlu: 4.089 ± 0.069
2.479ValPhe: 2.479 ± 0.047
5.562ValGly: 5.562 ± 0.074
1.434ValHis: 1.434 ± 0.043
3.991ValIle: 3.991 ± 0.06
2.085ValLys: 2.085 ± 0.045
7.33ValLeu: 7.33 ± 0.09
1.79ValMet: 1.79 ± 0.041
1.943ValAsn: 1.943 ± 0.039
4.201ValPro: 4.201 ± 0.061
2.116ValGln: 2.116 ± 0.047
5.166ValArg: 5.166 ± 0.072
4.047ValSer: 4.047 ± 0.06
4.558ValThr: 4.558 ± 0.071
5.558ValVal: 5.558 ± 0.086
1.0ValTrp: 1.0 ± 0.031
1.445ValTyr: 1.445 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.415TrpAla: 1.415 ± 0.033
0.128TrpCys: 0.128 ± 0.01
0.808TrpAsp: 0.808 ± 0.026
0.617TrpGlu: 0.617 ± 0.028
0.603TrpPhe: 0.603 ± 0.024
1.065TrpGly: 1.065 ± 0.035
0.374TrpHis: 0.374 ± 0.018
0.701TrpIle: 0.701 ± 0.025
0.504TrpLys: 0.504 ± 0.019
1.799TrpLeu: 1.799 ± 0.043
0.358TrpMet: 0.358 ± 0.019
0.464TrpAsn: 0.464 ± 0.02
0.728TrpPro: 0.728 ± 0.026
0.735TrpGln: 0.735 ± 0.024
1.3TrpArg: 1.3 ± 0.033
0.976TrpSer: 0.976 ± 0.028
0.835TrpThr: 0.835 ± 0.029
0.869TrpVal: 0.869 ± 0.033
0.26TrpTrp: 0.26 ± 0.016
0.321TrpTyr: 0.321 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.753TyrAla: 2.753 ± 0.056
0.233TyrCys: 0.233 ± 0.013
1.483TyrAsp: 1.483 ± 0.054
1.161TyrGlu: 1.161 ± 0.034
0.889TyrPhe: 0.889 ± 0.027
2.137TyrGly: 2.137 ± 0.047
0.579TyrHis: 0.579 ± 0.023
0.892TyrIle: 0.892 ± 0.03
0.627TyrLys: 0.627 ± 0.027
2.109TyrLeu: 2.109 ± 0.046
0.421TyrMet: 0.421 ± 0.02
0.698TyrAsn: 0.698 ± 0.028
1.124TyrPro: 1.124 ± 0.034
0.729TyrGln: 0.729 ± 0.022
1.784TyrArg: 1.784 ± 0.036
1.184TyrSer: 1.184 ± 0.048
1.189TyrThr: 1.189 ± 0.038
1.625TyrVal: 1.625 ± 0.038
0.344TyrTrp: 0.344 ± 0.015
0.619TyrTyr: 0.619 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3654 proteins (1213131 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski