Streptococcus phage Javan275

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AB95|A0A4D6AB95_9CAUD DNA helicase OS=Streptococcus phage Javan275 OX=2548090 GN=Javan275_0037 PE=3 SV=1
MM1 pKa = 7.97EE2 pKa = 4.6YY3 pKa = 8.11TTEE6 pKa = 4.07LNGKK10 pKa = 8.7KK11 pKa = 10.11LIYY14 pKa = 10.32GPLVEE19 pKa = 5.0NSNFTAKK26 pKa = 9.37EE27 pKa = 3.44WSAIYY32 pKa = 10.82AEE34 pKa = 4.49TVKK37 pKa = 10.67QNQPEE42 pKa = 4.28VFGTKK47 pKa = 10.21KK48 pKa = 10.3NDD50 pKa = 3.12KK51 pKa = 10.7DD52 pKa = 4.12YY53 pKa = 11.3IDD55 pKa = 3.84VFGALISLEE64 pKa = 3.77EE65 pKa = 4.28RR66 pKa = 11.84YY67 pKa = 9.72EE68 pKa = 4.0VLLEE72 pKa = 3.91NLPQNSYY79 pKa = 10.97SKK81 pKa = 11.04AGTHH85 pKa = 5.96PMWVANAVEE94 pKa = 4.88DD95 pKa = 3.66NTLNRR100 pKa = 11.84EE101 pKa = 3.87ATTADD106 pKa = 2.99ISEE109 pKa = 4.35MIEE112 pKa = 3.81NCEE115 pKa = 4.0SLEE118 pKa = 3.95EE119 pKa = 4.01LVEE122 pKa = 4.01EE123 pKa = 4.05LTDD126 pKa = 3.74YY127 pKa = 11.5FEE129 pKa = 6.26LEE131 pKa = 4.24VEE133 pKa = 4.29

Molecular weight: 15.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ABA6|A0A4D6ABA6_9CAUD Integrase OS=Streptococcus phage Javan275 OX=2548090 GN=Javan275_0047 PE=4 SV=1
MM1 pKa = 7.54ASVRR5 pKa = 11.84KK6 pKa = 8.96RR7 pKa = 11.84GKK9 pKa = 7.94YY10 pKa = 8.49FEE12 pKa = 4.15YY13 pKa = 10.04RR14 pKa = 11.84VVYY17 pKa = 10.1RR18 pKa = 11.84DD19 pKa = 3.5SLGEE23 pKa = 3.85RR24 pKa = 11.84HH25 pKa = 5.95EE26 pKa = 4.34ATSGGFKK33 pKa = 10.12TKK35 pKa = 10.44AQARR39 pKa = 11.84AAGQQRR45 pKa = 11.84EE46 pKa = 4.36VEE48 pKa = 4.38LRR50 pKa = 11.84RR51 pKa = 11.84SPLSNTDD58 pKa = 2.8VTMLDD63 pKa = 4.32YY64 pKa = 11.57YY65 pKa = 9.24MAWAQLYY72 pKa = 9.8KK73 pKa = 10.01KK74 pKa = 9.08TILRR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 9.96HH81 pKa = 6.45GILTNRR87 pKa = 11.84PRR89 pKa = 11.84ITLLSILVKK98 pKa = 10.85

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

9041

40

934

188.4

21.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.758 ± 0.465

0.509 ± 0.119

6.205 ± 0.319

7.643 ± 0.514

4.269 ± 0.367

5.785 ± 0.397

1.471 ± 0.198

7.499 ± 0.38

7.931 ± 0.346

8.373 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.721 ± 0.152

5.995 ± 0.339

2.577 ± 0.186

3.893 ± 0.259

4.015 ± 0.385

6.603 ± 0.436

6.393 ± 0.371

6.238 ± 0.243

1.106 ± 0.142

4.015 ± 0.382
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski