Escherichia virus ECH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Braunvirinae; Guelphvirus; unclassified Guelphvirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D2V1|A0A5P8D2V1_9CAUD VRR-NUC domain-containing protein OS=Escherichia virus ECH1 OX=2593465 GN=ECH1_51 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.32NVFGISYY9 pKa = 10.03EE10 pKa = 4.19EE11 pKa = 4.32EE12 pKa = 4.01FVDD15 pKa = 4.82CLIYY19 pKa = 10.94LNEE22 pKa = 4.02IYY24 pKa = 10.64GDD26 pKa = 3.78EE27 pKa = 4.45FEE29 pKa = 5.62CDD31 pKa = 3.47GDD33 pKa = 4.1SLHH36 pKa = 7.26AKK38 pKa = 9.97IEE40 pKa = 4.13LEE42 pKa = 4.2TFGEE46 pKa = 4.91VVV48 pKa = 2.94

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D5G7|A0A5P8D5G7_9CAUD Uncharacterized protein OS=Escherichia virus ECH1 OX=2593465 GN=ECH1_06 PE=4 SV=1
MM1 pKa = 7.15QKK3 pKa = 10.1LRR5 pKa = 11.84KK6 pKa = 9.72LPVFFLSPIDD16 pKa = 3.83FFNRR20 pKa = 11.84MHH22 pKa = 5.85HH23 pKa = 4.85TKK25 pKa = 9.41MRR27 pKa = 11.84IACFKK32 pKa = 9.64FAYY35 pKa = 9.4KK36 pKa = 10.4EE37 pKa = 4.0RR38 pKa = 11.84VHH40 pKa = 6.23NAFMIPVVLGNLINSTFAKK59 pKa = 10.56DD60 pKa = 3.21ILNRR64 pKa = 11.84CAYY67 pKa = 9.54TSFHH71 pKa = 6.18RR72 pKa = 11.84HH73 pKa = 4.77GSRR76 pKa = 11.84LL77 pKa = 3.43

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

13832

32

1158

184.4

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.09 ± 0.397

1.403 ± 0.157

5.805 ± 0.268

6.174 ± 0.33

4.049 ± 0.21

7.251 ± 0.3

1.612 ± 0.195

6.767 ± 0.208

6.514 ± 0.447

7.526 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.209

5.155 ± 0.275

3.159 ± 0.212

3.875 ± 0.344

5.249 ± 0.246

6.745 ± 0.198

5.682 ± 0.327

7.114 ± 0.291

1.417 ± 0.122

3.716 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski