Amino acid dipepetide frequency for Escherichia virus ECH1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.447AlaAla: 7.447 ± 1.238
0.651AlaCys: 0.651 ± 0.206
4.193AlaAsp: 4.193 ± 0.699
4.338AlaGlu: 4.338 ± 0.687
3.326AlaPhe: 3.326 ± 0.589
5.35AlaGly: 5.35 ± 0.752
1.518AlaHis: 1.518 ± 0.464
5.784AlaIle: 5.784 ± 0.632
5.929AlaLys: 5.929 ± 0.987
7.302AlaLeu: 7.302 ± 1.016
2.747AlaMet: 2.747 ± 0.49
3.543AlaAsn: 3.543 ± 0.539
2.314AlaPro: 2.314 ± 0.423
3.543AlaGln: 3.543 ± 0.635
4.7AlaArg: 4.7 ± 0.714
5.712AlaSer: 5.712 ± 0.641
4.555AlaThr: 4.555 ± 0.69
5.133AlaVal: 5.133 ± 0.814
0.94AlaTrp: 0.94 ± 0.309
1.88AlaTyr: 1.88 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.795CysAla: 0.795 ± 0.251
0.434CysCys: 0.434 ± 0.242
0.94CysAsp: 0.94 ± 0.246
1.085CysGlu: 1.085 ± 0.363
0.362CysPhe: 0.362 ± 0.16
1.374CysGly: 1.374 ± 0.442
0.145CysHis: 0.145 ± 0.103
0.289CysIle: 0.289 ± 0.162
0.795CysLys: 0.795 ± 0.264
1.301CysLeu: 1.301 ± 0.284
0.795CysMet: 0.795 ± 0.333
1.012CysAsn: 1.012 ± 0.328
0.217CysPro: 0.217 ± 0.152
0.362CysGln: 0.362 ± 0.152
0.362CysArg: 0.362 ± 0.138
1.663CysSer: 1.663 ± 0.452
0.795CysThr: 0.795 ± 0.232
0.868CysVal: 0.868 ± 0.297
0.217CysTrp: 0.217 ± 0.131
0.217CysTyr: 0.217 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
4.555AspAla: 4.555 ± 0.938
0.578AspCys: 0.578 ± 0.2
4.049AspAsp: 4.049 ± 0.683
3.832AspGlu: 3.832 ± 0.586
2.458AspPhe: 2.458 ± 0.417
6.29AspGly: 6.29 ± 0.625
0.795AspHis: 0.795 ± 0.319
3.832AspIle: 3.832 ± 0.54
4.483AspLys: 4.483 ± 0.587
3.687AspLeu: 3.687 ± 0.549
1.518AspMet: 1.518 ± 0.361
2.964AspAsn: 2.964 ± 0.68
1.663AspPro: 1.663 ± 0.364
1.229AspGln: 1.229 ± 0.263
1.808AspArg: 1.808 ± 0.341
3.615AspSer: 3.615 ± 0.618
2.314AspThr: 2.314 ± 0.519
3.977AspVal: 3.977 ± 0.474
1.663AspTrp: 1.663 ± 0.485
2.675AspTyr: 2.675 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
5.061GluAla: 5.061 ± 0.752
0.94GluCys: 0.94 ± 0.385
2.675GluAsp: 2.675 ± 0.463
3.687GluGlu: 3.687 ± 0.771
3.615GluPhe: 3.615 ± 0.598
3.543GluGly: 3.543 ± 0.488
0.506GluHis: 0.506 ± 0.211
4.7GluIle: 4.7 ± 0.724
3.832GluLys: 3.832 ± 0.644
5.133GluLeu: 5.133 ± 0.565
2.097GluMet: 2.097 ± 0.434
3.109GluAsn: 3.109 ± 0.401
1.952GluPro: 1.952 ± 0.311
2.458GluGln: 2.458 ± 0.695
2.964GluArg: 2.964 ± 0.556
3.76GluSer: 3.76 ± 0.491
3.037GluThr: 3.037 ± 0.435
5.423GluVal: 5.423 ± 0.704
0.723GluTrp: 0.723 ± 0.292
2.531GluTyr: 2.531 ± 0.553
0.0GluXaa: 0.0 ± 0.0
Phe
1.952PheAla: 1.952 ± 0.411
0.651PheCys: 0.651 ± 0.2
2.964PheAsp: 2.964 ± 0.429
3.543PheGlu: 3.543 ± 0.621
1.374PhePhe: 1.374 ± 0.353
3.615PheGly: 3.615 ± 0.561
0.723PheHis: 0.723 ± 0.336
2.747PheIle: 2.747 ± 0.53
2.603PheLys: 2.603 ± 0.482
2.603PheLeu: 2.603 ± 0.569
1.157PheMet: 1.157 ± 0.434
1.591PheAsn: 1.591 ± 0.423
1.085PhePro: 1.085 ± 0.269
2.097PheGln: 2.097 ± 0.569
2.314PheArg: 2.314 ± 0.623
2.892PheSer: 2.892 ± 0.584
2.386PheThr: 2.386 ± 0.428
2.892PheVal: 2.892 ± 0.548
0.289PheTrp: 0.289 ± 0.127
1.012PheTyr: 1.012 ± 0.25
0.0PheXaa: 0.0 ± 0.0
Gly
5.495GlyAla: 5.495 ± 0.946
1.301GlyCys: 1.301 ± 0.301
4.049GlyAsp: 4.049 ± 0.516
4.121GlyGlu: 4.121 ± 0.552
3.326GlyPhe: 3.326 ± 0.472
6.652GlyGly: 6.652 ± 1.226
0.795GlyHis: 0.795 ± 0.326
4.772GlyIle: 4.772 ± 0.562
5.495GlyLys: 5.495 ± 0.988
6.796GlyLeu: 6.796 ± 0.93
1.735GlyMet: 1.735 ± 0.361
3.687GlyAsn: 3.687 ± 0.59
0.506GlyPro: 0.506 ± 0.216
2.169GlyGln: 2.169 ± 0.39
2.964GlyArg: 2.964 ± 0.502
5.206GlySer: 5.206 ± 0.63
3.037GlyThr: 3.037 ± 0.511
5.567GlyVal: 5.567 ± 0.627
1.157GlyTrp: 1.157 ± 0.326
3.687GlyTyr: 3.687 ± 0.696
0.0GlyXaa: 0.0 ± 0.0
His
0.795HisAla: 0.795 ± 0.284
0.145HisCys: 0.145 ± 0.101
0.94HisAsp: 0.94 ± 0.272
0.651HisGlu: 0.651 ± 0.254
0.578HisPhe: 0.578 ± 0.296
1.229HisGly: 1.229 ± 0.404
0.578HisHis: 0.578 ± 0.187
1.157HisIle: 1.157 ± 0.386
1.085HisLys: 1.085 ± 0.28
1.735HisLeu: 1.735 ± 0.506
0.506HisMet: 0.506 ± 0.256
1.085HisAsn: 1.085 ± 0.314
0.217HisPro: 0.217 ± 0.11
1.157HisGln: 1.157 ± 0.338
0.723HisArg: 0.723 ± 0.28
0.94HisSer: 0.94 ± 0.248
1.085HisThr: 1.085 ± 0.384
0.795HisVal: 0.795 ± 0.267
0.217HisTrp: 0.217 ± 0.133
0.506HisTyr: 0.506 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
6.724IleAla: 6.724 ± 1.128
1.301IleCys: 1.301 ± 0.405
5.133IleAsp: 5.133 ± 0.559
3.687IleGlu: 3.687 ± 0.533
1.518IlePhe: 1.518 ± 0.369
4.483IleGly: 4.483 ± 0.587
1.518IleHis: 1.518 ± 0.375
3.109IleIle: 3.109 ± 0.521
4.844IleLys: 4.844 ± 0.79
4.627IleLeu: 4.627 ± 0.629
1.301IleMet: 1.301 ± 0.346
4.41IleAsn: 4.41 ± 0.544
3.037IlePro: 3.037 ± 0.541
2.024IleGln: 2.024 ± 0.531
3.109IleArg: 3.109 ± 0.429
4.7IleSer: 4.7 ± 0.602
4.338IleThr: 4.338 ± 0.516
4.193IleVal: 4.193 ± 0.631
0.868IleTrp: 0.868 ± 0.354
2.747IleTyr: 2.747 ± 0.577
0.0IleXaa: 0.0 ± 0.0
Lys
6.073LysAla: 6.073 ± 0.892
0.578LysCys: 0.578 ± 0.254
3.832LysAsp: 3.832 ± 0.538
5.35LysGlu: 5.35 ± 1.089
2.675LysPhe: 2.675 ± 0.487
4.049LysGly: 4.049 ± 0.652
0.94LysHis: 0.94 ± 0.262
4.7LysIle: 4.7 ± 0.678
3.76LysLys: 3.76 ± 0.724
6.073LysLeu: 6.073 ± 0.785
1.88LysMet: 1.88 ± 0.415
1.591LysAsn: 1.591 ± 0.295
2.024LysPro: 2.024 ± 0.431
2.531LysGln: 2.531 ± 0.477
2.964LysArg: 2.964 ± 0.706
4.41LysSer: 4.41 ± 0.663
3.326LysThr: 3.326 ± 0.459
4.916LysVal: 4.916 ± 0.618
0.651LysTrp: 0.651 ± 0.262
2.82LysTyr: 2.82 ± 0.481
0.0LysXaa: 0.0 ± 0.0
Leu
6.869LeuAla: 6.869 ± 0.792
1.012LeuCys: 1.012 ± 0.3
3.76LeuAsp: 3.76 ± 0.614
4.41LeuGlu: 4.41 ± 0.62
2.386LeuPhe: 2.386 ± 0.48
3.832LeuGly: 3.832 ± 0.67
1.085LeuHis: 1.085 ± 0.298
6.218LeuIle: 6.218 ± 0.598
4.049LeuLys: 4.049 ± 0.493
4.338LeuLeu: 4.338 ± 0.606
2.024LeuMet: 2.024 ± 0.638
3.977LeuAsn: 3.977 ± 0.463
3.181LeuPro: 3.181 ± 0.431
3.037LeuGln: 3.037 ± 0.652
5.495LeuArg: 5.495 ± 0.793
6.073LeuSer: 6.073 ± 0.704
5.423LeuThr: 5.423 ± 0.726
5.278LeuVal: 5.278 ± 0.68
0.723LeuTrp: 0.723 ± 0.276
2.097LeuTyr: 2.097 ± 0.522
0.0LeuXaa: 0.0 ± 0.0
Met
2.314MetAla: 2.314 ± 0.445
0.362MetCys: 0.362 ± 0.187
1.157MetAsp: 1.157 ± 0.408
1.591MetGlu: 1.591 ± 0.306
1.301MetPhe: 1.301 ± 0.386
0.651MetGly: 0.651 ± 0.219
0.217MetHis: 0.217 ± 0.142
2.097MetIle: 2.097 ± 0.422
1.518MetLys: 1.518 ± 0.309
1.663MetLeu: 1.663 ± 0.415
1.229MetMet: 1.229 ± 0.342
1.229MetAsn: 1.229 ± 0.368
0.651MetPro: 0.651 ± 0.264
0.723MetGln: 0.723 ± 0.238
2.531MetArg: 2.531 ± 0.392
1.88MetSer: 1.88 ± 0.41
2.82MetThr: 2.82 ± 0.461
2.024MetVal: 2.024 ± 0.426
0.434MetTrp: 0.434 ± 0.202
0.578MetTyr: 0.578 ± 0.225
0.0MetXaa: 0.0 ± 0.0
Asn
4.121AsnAla: 4.121 ± 0.688
0.506AsnCys: 0.506 ± 0.184
2.675AsnAsp: 2.675 ± 0.491
3.398AsnGlu: 3.398 ± 0.724
1.88AsnPhe: 1.88 ± 0.341
5.133AsnGly: 5.133 ± 0.99
1.012AsnHis: 1.012 ± 0.258
2.169AsnIle: 2.169 ± 0.369
2.82AsnLys: 2.82 ± 0.516
4.338AsnLeu: 4.338 ± 0.666
0.868AsnMet: 0.868 ± 0.271
3.254AsnAsn: 3.254 ± 0.531
2.097AsnPro: 2.097 ± 0.429
2.097AsnGln: 2.097 ± 0.406
2.169AsnArg: 2.169 ± 0.507
3.687AsnSer: 3.687 ± 0.693
2.747AsnThr: 2.747 ± 0.674
3.47AsnVal: 3.47 ± 0.412
1.085AsnTrp: 1.085 ± 0.243
2.024AsnTyr: 2.024 ± 0.459
0.0AsnXaa: 0.0 ± 0.0
Pro
3.398ProAla: 3.398 ± 0.411
0.723ProCys: 0.723 ± 0.236
1.808ProAsp: 1.808 ± 0.404
2.169ProGlu: 2.169 ± 0.439
1.446ProPhe: 1.446 ± 0.32
2.241ProGly: 2.241 ± 0.408
0.723ProHis: 0.723 ± 0.285
2.675ProIle: 2.675 ± 0.527
1.374ProLys: 1.374 ± 0.346
1.591ProLeu: 1.591 ± 0.384
0.795ProMet: 0.795 ± 0.244
1.518ProAsn: 1.518 ± 0.478
0.434ProPro: 0.434 ± 0.211
1.085ProGln: 1.085 ± 0.315
1.735ProArg: 1.735 ± 0.339
1.446ProSer: 1.446 ± 0.384
1.808ProThr: 1.808 ± 0.443
2.603ProVal: 2.603 ± 0.468
0.506ProTrp: 0.506 ± 0.261
1.518ProTyr: 1.518 ± 0.299
0.0ProXaa: 0.0 ± 0.0
Gln
3.76GlnAla: 3.76 ± 0.882
0.362GlnCys: 0.362 ± 0.154
1.518GlnAsp: 1.518 ± 0.342
2.531GlnGlu: 2.531 ± 0.465
1.518GlnPhe: 1.518 ± 0.435
2.241GlnGly: 2.241 ± 0.492
0.651GlnHis: 0.651 ± 0.252
3.398GlnIle: 3.398 ± 0.581
2.892GlnLys: 2.892 ± 0.481
2.82GlnLeu: 2.82 ± 0.564
0.795GlnMet: 0.795 ± 0.287
2.169GlnAsn: 2.169 ± 0.533
0.868GlnPro: 0.868 ± 0.257
2.531GlnGln: 2.531 ± 0.723
1.735GlnArg: 1.735 ± 0.429
2.82GlnSer: 2.82 ± 0.683
1.952GlnThr: 1.952 ± 0.395
2.169GlnVal: 2.169 ± 0.389
0.723GlnTrp: 0.723 ± 0.312
1.301GlnTyr: 1.301 ± 0.27
0.0GlnXaa: 0.0 ± 0.0
Arg
4.121ArgAla: 4.121 ± 0.601
1.012ArgCys: 1.012 ± 0.368
1.735ArgAsp: 1.735 ± 0.303
3.037ArgGlu: 3.037 ± 0.519
1.952ArgPhe: 1.952 ± 0.364
2.747ArgGly: 2.747 ± 0.454
0.868ArgHis: 0.868 ± 0.268
3.977ArgIle: 3.977 ± 0.566
4.266ArgLys: 4.266 ± 0.534
3.977ArgLeu: 3.977 ± 0.433
1.663ArgMet: 1.663 ± 0.379
3.181ArgAsn: 3.181 ± 0.562
1.952ArgPro: 1.952 ± 0.494
1.952ArgGln: 1.952 ± 0.441
3.109ArgArg: 3.109 ± 0.553
3.109ArgSer: 3.109 ± 0.554
2.024ArgThr: 2.024 ± 0.452
4.193ArgVal: 4.193 ± 0.584
0.868ArgTrp: 0.868 ± 0.313
2.747ArgTyr: 2.747 ± 0.439
0.0ArgXaa: 0.0 ± 0.0
Ser
4.193SerAla: 4.193 ± 0.529
0.795SerCys: 0.795 ± 0.259
4.989SerAsp: 4.989 ± 0.699
4.266SerGlu: 4.266 ± 0.558
2.964SerPhe: 2.964 ± 0.46
6.001SerGly: 6.001 ± 0.459
1.374SerHis: 1.374 ± 0.352
4.844SerIle: 4.844 ± 0.609
3.037SerLys: 3.037 ± 0.506
5.423SerLeu: 5.423 ± 0.77
1.229SerMet: 1.229 ± 0.336
3.326SerAsn: 3.326 ± 0.489
2.241SerPro: 2.241 ± 0.51
2.458SerGln: 2.458 ± 0.558
3.181SerArg: 3.181 ± 0.453
3.254SerSer: 3.254 ± 0.696
5.061SerThr: 5.061 ± 1.001
5.64SerVal: 5.64 ± 0.639
0.723SerTrp: 0.723 ± 0.252
2.458SerTyr: 2.458 ± 0.48
0.0SerXaa: 0.0 ± 0.0
Thr
4.627ThrAla: 4.627 ± 0.816
0.651ThrCys: 0.651 ± 0.28
2.531ThrAsp: 2.531 ± 0.469
2.892ThrGlu: 2.892 ± 0.457
2.675ThrPhe: 2.675 ± 0.479
6.001ThrGly: 6.001 ± 0.821
0.94ThrHis: 0.94 ± 0.265
3.615ThrIle: 3.615 ± 0.452
3.687ThrLys: 3.687 ± 0.588
3.109ThrLeu: 3.109 ± 0.426
1.591ThrMet: 1.591 ± 0.339
2.675ThrAsn: 2.675 ± 0.482
2.675ThrPro: 2.675 ± 0.482
2.892ThrGln: 2.892 ± 0.597
2.892ThrArg: 2.892 ± 0.576
3.398ThrSer: 3.398 ± 0.462
3.254ThrThr: 3.254 ± 0.571
3.47ThrVal: 3.47 ± 0.559
0.651ThrTrp: 0.651 ± 0.25
2.603ThrTyr: 2.603 ± 0.503
0.0ThrXaa: 0.0 ± 0.0
Val
5.206ValAla: 5.206 ± 0.801
1.229ValCys: 1.229 ± 0.367
5.133ValAsp: 5.133 ± 0.514
3.543ValGlu: 3.543 ± 0.585
3.47ValPhe: 3.47 ± 0.489
3.687ValGly: 3.687 ± 0.618
0.578ValHis: 0.578 ± 0.218
4.844ValIle: 4.844 ± 0.723
4.989ValLys: 4.989 ± 0.684
4.555ValLeu: 4.555 ± 0.674
2.169ValMet: 2.169 ± 0.442
4.555ValAsn: 4.555 ± 0.59
2.386ValPro: 2.386 ± 0.459
2.314ValGln: 2.314 ± 0.631
4.627ValArg: 4.627 ± 0.651
4.844ValSer: 4.844 ± 0.661
4.555ValThr: 4.555 ± 0.853
5.206ValVal: 5.206 ± 0.924
0.868ValTrp: 0.868 ± 0.312
2.386ValTyr: 2.386 ± 0.417
0.0ValXaa: 0.0 ± 0.0
Trp
1.085TrpAla: 1.085 ± 0.3
0.289TrpCys: 0.289 ± 0.157
1.012TrpAsp: 1.012 ± 0.244
0.578TrpGlu: 0.578 ± 0.271
0.723TrpPhe: 0.723 ± 0.229
0.723TrpGly: 0.723 ± 0.227
0.578TrpHis: 0.578 ± 0.262
0.868TrpIle: 0.868 ± 0.251
1.085TrpLys: 1.085 ± 0.482
1.518TrpLeu: 1.518 ± 0.43
0.217TrpMet: 0.217 ± 0.134
0.578TrpAsn: 0.578 ± 0.195
0.506TrpPro: 0.506 ± 0.211
0.145TrpGln: 0.145 ± 0.102
1.229TrpArg: 1.229 ± 0.422
1.012TrpSer: 1.012 ± 0.443
0.362TrpThr: 0.362 ± 0.175
1.012TrpVal: 1.012 ± 0.277
0.145TrpTrp: 0.145 ± 0.095
0.506TrpTyr: 0.506 ± 0.265
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.314TyrAla: 2.314 ± 0.531
0.506TyrCys: 0.506 ± 0.186
2.964TyrAsp: 2.964 ± 0.576
3.037TyrGlu: 3.037 ± 0.565
0.94TyrPhe: 0.94 ± 0.315
2.675TyrGly: 2.675 ± 0.469
0.578TyrHis: 0.578 ± 0.236
1.88TyrIle: 1.88 ± 0.359
2.603TyrLys: 2.603 ± 0.589
2.675TyrLeu: 2.675 ± 0.465
0.578TyrMet: 0.578 ± 0.189
2.097TyrAsn: 2.097 ± 0.388
1.591TyrPro: 1.591 ± 0.442
1.952TyrGln: 1.952 ± 0.577
1.88TyrArg: 1.88 ± 0.435
3.037TyrSer: 3.037 ± 0.507
1.952TyrThr: 1.952 ± 0.353
2.314TyrVal: 2.314 ± 0.447
0.723TyrTrp: 0.723 ± 0.231
0.868TyrTyr: 0.868 ± 0.265
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (13832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski