Rhizobium sp. Root149

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5002 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7YYJ0|A0A0Q7YYJ0_9RHIZ DNA topoisomerase 1 OS=Rhizobium sp. Root149 OX=1736473 GN=topA PE=3 SV=1
MM1 pKa = 7.61WGGSGNDD8 pKa = 2.88HH9 pKa = 6.98YY10 pKa = 11.48YY11 pKa = 11.1FNGQGFDD18 pKa = 4.13RR19 pKa = 11.84INDD22 pKa = 3.67GVTNTGAARR31 pKa = 11.84TDD33 pKa = 3.49GAFDD37 pKa = 3.87TEE39 pKa = 4.32DD40 pKa = 3.39VLYY43 pKa = 10.89VSYY46 pKa = 10.83AANDD50 pKa = 3.57LGLNRR55 pKa = 11.84IGNDD59 pKa = 3.16LVIFSNADD67 pKa = 3.09AVDD70 pKa = 4.38NILNSSVVIEE80 pKa = 4.01NFFLGSHH87 pKa = 5.29YY88 pKa = 10.67VVEE91 pKa = 4.87VVATSSGAGPAYY103 pKa = 10.56DD104 pKa = 3.93LTGLLAAA111 pKa = 5.71

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7Y1N7|A0A0Q7Y1N7_9RHIZ Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO OS=Rhizobium sp. Root149 OX=1736473 GN=trmFO PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 8.98VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5002

0

5002

1581867

41

4159

316.2

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.516 ± 0.046

0.807 ± 0.011

5.594 ± 0.028

5.838 ± 0.034

3.886 ± 0.023

8.142 ± 0.035

2.023 ± 0.016

5.682 ± 0.028

3.833 ± 0.03

10.119 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.015

2.921 ± 0.02

4.819 ± 0.023

3.438 ± 0.025

6.604 ± 0.035

6.014 ± 0.031

5.371 ± 0.026

7.254 ± 0.028

1.251 ± 0.014

2.248 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski