Amino acid dipepetide frequency for Rhizobium sp. Root149

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.575AlaAla: 14.575 ± 0.166	0.997AlaCys: 0.997 ± 0.026	6.371AlaAsp: 6.371 ± 0.071	7.179AlaGlu: 7.179 ± 0.088	4.458AlaPhe: 4.458 ± 0.061	9.381AlaGly: 9.381 ± 0.086	2.043AlaHis: 2.043 ± 0.035	6.678AlaIle: 6.678 ± 0.069	4.523AlaLys: 4.523 ± 0.068	12.262AlaLeu: 12.262 ± 0.113	3.492AlaMet: 3.492 ± 0.054	3.149AlaAsn: 3.149 ± 0.047	4.551AlaPro: 4.551 ± 0.067	3.957AlaGln: 3.957 ± 0.061	7.233AlaArg: 7.233 ± 0.068	6.858AlaSer: 6.858 ± 0.082	5.607AlaThr: 5.607 ± 0.059	8.12AlaVal: 8.12 ± 0.076	1.252AlaTrp: 1.252 ± 0.031	2.468AlaTyr: 2.468 ± 0.042	0.001AlaXaa: 0.001 ± 0.001
Cys	0.85CysAla: 0.85 ± 0.025	0.113CysCys: 0.113 ± 0.009	0.501CysAsp: 0.501 ± 0.018	0.444CysGlu: 0.444 ± 0.018	0.314CysPhe: 0.314 ± 0.014	0.853CysGly: 0.853 ± 0.024	0.221CysHis: 0.221 ± 0.014	0.403CysIle: 0.403 ± 0.016	0.209CysLys: 0.209 ± 0.011	0.833CysLeu: 0.833 ± 0.024	0.168CysMet: 0.168 ± 0.01	0.204CysAsn: 0.204 ± 0.012	0.402CysPro: 0.402 ± 0.018	0.26CysGln: 0.26 ± 0.012	0.557CysArg: 0.557 ± 0.02	0.468CysSer: 0.468 ± 0.019	0.381CysThr: 0.381 ± 0.014	0.59CysVal: 0.59 ± 0.018	0.106CysTrp: 0.106 ± 0.007	0.188CysTyr: 0.188 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.278AspAla: 6.278 ± 0.069	0.473AspCys: 0.473 ± 0.018	2.98AspAsp: 2.98 ± 0.05	3.492AspGlu: 3.492 ± 0.045	2.203AspPhe: 2.203 ± 0.039	4.824AspGly: 4.824 ± 0.062	1.29AspHis: 1.29 ± 0.03	3.227AspIle: 3.227 ± 0.051	1.933AspLys: 1.933 ± 0.041	6.066AspLeu: 6.066 ± 0.073	1.412AspMet: 1.412 ± 0.033	1.39AspAsn: 1.39 ± 0.031	3.163AspPro: 3.163 ± 0.044	1.93AspGln: 1.93 ± 0.043	4.085AspArg: 4.085 ± 0.05	2.158AspSer: 2.158 ± 0.035	2.46AspThr: 2.46 ± 0.037	4.15AspVal: 4.15 ± 0.05	0.899AspTrp: 0.899 ± 0.025	1.52AspTyr: 1.52 ± 0.033	0.002AspXaa: 0.002 ± 0.001
Glu	7.363GluAla: 7.363 ± 0.078	0.369GluCys: 0.369 ± 0.017	2.928GluAsp: 2.928 ± 0.046	3.567GluGlu: 3.567 ± 0.057	1.959GluPhe: 1.959 ± 0.039	4.24GluGly: 4.24 ± 0.058	1.202GluHis: 1.202 ± 0.025	3.788GluIle: 3.788 ± 0.049	2.77GluLys: 2.77 ± 0.049	5.504GluLeu: 5.504 ± 0.06	1.599GluMet: 1.599 ± 0.028	1.904GluAsn: 1.904 ± 0.039	2.585GluPro: 2.585 ± 0.04	2.367GluGln: 2.367 ± 0.045	4.672GluArg: 4.672 ± 0.063	2.39GluSer: 2.39 ± 0.04	3.629GluThr: 3.629 ± 0.055	3.826GluVal: 3.826 ± 0.05	0.718GluTrp: 0.718 ± 0.025	0.994GluTyr: 0.994 ± 0.031	0.001GluXaa: 0.001 ± 0.001
Phe	4.344PheAla: 4.344 ± 0.059	0.384PheCys: 0.384 ± 0.015	2.666PheAsp: 2.666 ± 0.046	2.277PheGlu: 2.277 ± 0.039	1.57PhePhe: 1.57 ± 0.043	3.739PheGly: 3.739 ± 0.057	0.787PheHis: 0.787 ± 0.024	1.997PheIle: 1.997 ± 0.043	1.278PheLys: 1.278 ± 0.034	3.531PheLeu: 3.531 ± 0.06	0.919PheMet: 0.919 ± 0.024	1.245PheAsn: 1.245 ± 0.03	1.588PhePro: 1.588 ± 0.035	1.188PheGln: 1.188 ± 0.024	2.295PheArg: 2.295 ± 0.038	2.566PheSer: 2.566 ± 0.048	2.069PheThr: 2.069 ± 0.039	2.884PheVal: 2.884 ± 0.048	0.587PheTrp: 0.587 ± 0.021	0.944PheTyr: 0.944 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	7.968GlyAla: 7.968 ± 0.082	0.785GlyCys: 0.785 ± 0.024	4.012GlyAsp: 4.012 ± 0.047	4.742GlyGlu: 4.742 ± 0.065	3.703GlyPhe: 3.703 ± 0.048	6.673GlyGly: 6.673 ± 0.102	1.824GlyHis: 1.824 ± 0.038	4.839GlyIle: 4.839 ± 0.061	3.701GlyLys: 3.701 ± 0.054	8.714GlyLeu: 8.714 ± 0.095	2.254GlyMet: 2.254 ± 0.042	2.354GlyAsn: 2.354 ± 0.052	2.998GlyPro: 2.998 ± 0.045	2.967GlyGln: 2.967 ± 0.049	5.363GlyArg: 5.363 ± 0.061	4.961GlySer: 4.961 ± 0.058	4.365GlyThr: 4.365 ± 0.066	5.672GlyVal: 5.672 ± 0.065	1.189GlyTrp: 1.189 ± 0.032	2.333GlyTyr: 2.333 ± 0.037	0.001GlyXaa: 0.001 ± 0.001
His	2.132HisAla: 2.132 ± 0.046	0.209HisCys: 0.209 ± 0.011	1.178HisAsp: 1.178 ± 0.032	1.11HisGlu: 1.11 ± 0.029	0.887HisPhe: 0.887 ± 0.024	1.808HisGly: 1.808 ± 0.04	0.589HisHis: 0.589 ± 0.027	1.047HisIle: 1.047 ± 0.024	0.549HisLys: 0.549 ± 0.017	2.137HisLeu: 2.137 ± 0.04	0.522HisMet: 0.522 ± 0.02	0.503HisAsn: 0.503 ± 0.018	1.24HisPro: 1.24 ± 0.027	0.69HisGln: 0.69 ± 0.024	1.412HisArg: 1.412 ± 0.035	1.058HisSer: 1.058 ± 0.027	0.818HisThr: 0.818 ± 0.022	1.486HisVal: 1.486 ± 0.031	0.289HisTrp: 0.289 ± 0.011	0.57HisTyr: 0.57 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.388IleAla: 7.388 ± 0.071	0.587IleCys: 0.587 ± 0.02	3.696IleAsp: 3.696 ± 0.047	3.808IleGlu: 3.808 ± 0.048	2.032IlePhe: 2.032 ± 0.042	5.176IleGly: 5.176 ± 0.066	0.997IleHis: 0.997 ± 0.026	2.945IleIle: 2.945 ± 0.053	1.757IleLys: 1.757 ± 0.036	5.016IleLeu: 5.016 ± 0.066	1.175IleMet: 1.175 ± 0.034	1.719IleAsn: 1.719 ± 0.034	2.48IlePro: 2.48 ± 0.046	1.439IleGln: 1.439 ± 0.026	3.644IleArg: 3.644 ± 0.055	3.649IleSer: 3.649 ± 0.055	2.947IleThr: 2.947 ± 0.053	4.465IleVal: 4.465 ± 0.065	0.664IleTrp: 0.664 ± 0.021	1.237IleTyr: 1.237 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.944LysAla: 4.944 ± 0.067	0.172LysCys: 0.172 ± 0.01	2.08LysAsp: 2.08 ± 0.04	1.948LysGlu: 1.948 ± 0.043	1.08LysPhe: 1.08 ± 0.029	2.922LysGly: 2.922 ± 0.048	0.675LysHis: 0.675 ± 0.019	2.136LysIle: 2.136 ± 0.04	1.52LysLys: 1.52 ± 0.038	3.832LysLeu: 3.832 ± 0.056	0.962LysMet: 0.962 ± 0.024	1.127LysAsn: 1.127 ± 0.028	2.505LysPro: 2.505 ± 0.046	1.331LysGln: 1.331 ± 0.031	2.615LysArg: 2.615 ± 0.045	2.3LysSer: 2.3 ± 0.037	2.331LysThr: 2.331 ± 0.043	2.83LysVal: 2.83 ± 0.057	0.393LysTrp: 0.393 ± 0.016	0.628LysTyr: 0.628 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	12.457LeuAla: 12.457 ± 0.119	0.855LeuCys: 0.855 ± 0.025	5.801LeuAsp: 5.801 ± 0.06	5.482LeuGlu: 5.482 ± 0.061	3.773LeuPhe: 3.773 ± 0.06	7.891LeuGly: 7.891 ± 0.075	1.819LeuHis: 1.819 ± 0.034	5.483LeuIle: 5.483 ± 0.062	4.429LeuLys: 4.429 ± 0.064	9.51LeuLeu: 9.51 ± 0.116	2.603LeuMet: 2.603 ± 0.049	2.979LeuAsn: 2.979 ± 0.05	5.3LeuPro: 5.3 ± 0.064	3.13LeuGln: 3.13 ± 0.048	6.224LeuArg: 6.224 ± 0.072	7.361LeuSer: 7.361 ± 0.091	5.786LeuThr: 5.786 ± 0.075	7.218LeuVal: 7.218 ± 0.075	1.039LeuTrp: 1.039 ± 0.027	2.055LeuTyr: 2.055 ± 0.034	0.001LeuXaa: 0.001 ± 0.001
Met	3.144MetAla: 3.144 ± 0.045	0.148MetCys: 0.148 ± 0.01	1.238MetAsp: 1.238 ± 0.028	1.29MetGlu: 1.29 ± 0.03	0.781MetPhe: 0.781 ± 0.025	1.79MetGly: 1.79 ± 0.035	0.42MetHis: 0.42 ± 0.016	1.568MetIle: 1.568 ± 0.033	1.144MetLys: 1.144 ± 0.03	2.676MetLeu: 2.676 ± 0.042	0.762MetMet: 0.762 ± 0.022	0.89MetAsn: 0.89 ± 0.025	1.53MetPro: 1.53 ± 0.036	1.018MetGln: 1.018 ± 0.025	1.811MetArg: 1.811 ± 0.036	1.813MetSer: 1.813 ± 0.033	2.009MetThr: 2.009 ± 0.032	1.847MetVal: 1.847 ± 0.034	0.206MetTrp: 0.206 ± 0.013	0.313MetTyr: 0.313 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.392AsnAla: 3.392 ± 0.056	0.246AsnCys: 0.246 ± 0.013	1.538AsnAsp: 1.538 ± 0.034	1.517AsnGlu: 1.517 ± 0.031	1.103AsnPhe: 1.103 ± 0.029	2.71AsnGly: 2.71 ± 0.049	0.587AsnHis: 0.587 ± 0.019	1.62AsnIle: 1.62 ± 0.034	0.872AsnLys: 0.872 ± 0.029	3.007AsnLeu: 3.007 ± 0.048	0.723AsnMet: 0.723 ± 0.021	0.833AsnAsn: 0.833 ± 0.024	1.897AsnPro: 1.897 ± 0.036	0.908AsnGln: 0.908 ± 0.026	2.079AsnArg: 2.079 ± 0.035	1.508AsnSer: 1.508 ± 0.028	1.402AsnThr: 1.402 ± 0.031	2.064AsnVal: 2.064 ± 0.041	0.492AsnTrp: 0.492 ± 0.019	0.711AsnTyr: 0.711 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	5.291ProAla: 5.291 ± 0.074	0.29ProCys: 0.29 ± 0.013	3.373ProAsp: 3.373 ± 0.05	3.455ProGlu: 3.455 ± 0.053	1.943ProPhe: 1.943 ± 0.036	3.838ProGly: 3.838 ± 0.054	1.043ProHis: 1.043 ± 0.024	2.34ProIle: 2.34 ± 0.038	1.866ProLys: 1.866 ± 0.034	4.529ProLeu: 4.529 ± 0.054	1.212ProMet: 1.212 ± 0.029	1.407ProAsn: 1.407 ± 0.03	2.102ProPro: 2.102 ± 0.047	1.759ProGln: 1.759 ± 0.035	2.497ProArg: 2.497 ± 0.044	2.936ProSer: 2.936 ± 0.041	2.384ProThr: 2.384 ± 0.036	4.183ProVal: 4.183 ± 0.055	0.582ProTrp: 0.582 ± 0.02	1.164ProTyr: 1.164 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	4.206GlnAla: 4.206 ± 0.062	0.191GlnCys: 0.191 ± 0.012	1.668GlnAsp: 1.668 ± 0.035	1.773GlnGlu: 1.773 ± 0.041	1.153GlnPhe: 1.153 ± 0.027	2.431GlnGly: 2.431 ± 0.042	0.673GlnHis: 0.673 ± 0.02	2.178GlnIle: 2.178 ± 0.054	1.4GlnLys: 1.4 ± 0.031	3.144GlnLeu: 3.144 ± 0.051	1.018GlnMet: 1.018 ± 0.026	1.075GlnAsn: 1.075 ± 0.032	1.901GlnPro: 1.901 ± 0.045	1.501GlnGln: 1.501 ± 0.04	2.486GlnArg: 2.486 ± 0.042	2.171GlnSer: 2.171 ± 0.037	1.977GlnThr: 1.977 ± 0.039	2.399GlnVal: 2.399 ± 0.043	0.394GlnTrp: 0.394 ± 0.017	0.641GlnTyr: 0.641 ± 0.02	0.002GlnXaa: 0.002 ± 0.001
Arg	6.467ArgAla: 6.467 ± 0.07	0.443ArgCys: 0.443 ± 0.017	3.809ArgAsp: 3.809 ± 0.049	3.998ArgGlu: 3.998 ± 0.058	2.935ArgPhe: 2.935 ± 0.044	4.206ArgGly: 4.206 ± 0.057	1.624ArgHis: 1.624 ± 0.034	4.062ArgIle: 4.062 ± 0.05	2.608ArgLys: 2.608 ± 0.04	7.327ArgLeu: 7.327 ± 0.081	1.931ArgMet: 1.931 ± 0.035	1.999ArgAsn: 1.999 ± 0.034	3.051ArgPro: 3.051 ± 0.052	2.791ArgGln: 2.791 ± 0.041	4.859ArgArg: 4.859 ± 0.063	3.959ArgSer: 3.959 ± 0.059	3.194ArgThr: 3.194 ± 0.045	4.201ArgVal: 4.201 ± 0.053	0.867ArgTrp: 0.867 ± 0.024	1.706ArgTyr: 1.706 ± 0.037	0.001ArgXaa: 0.001 ± 0.001
Ser	6.523SerAla: 6.523 ± 0.075	0.435SerCys: 0.435 ± 0.018	3.244SerAsp: 3.244 ± 0.047	3.223SerGlu: 3.223 ± 0.05	2.647SerPhe: 2.647 ± 0.042	5.837SerGly: 5.837 ± 0.065	1.28SerHis: 1.28 ± 0.031	3.396SerIle: 3.396 ± 0.049	2.074SerLys: 2.074 ± 0.037	6.251SerLeu: 6.251 ± 0.086	1.587SerMet: 1.587 ± 0.033	1.675SerAsn: 1.675 ± 0.035	2.737SerPro: 2.737 ± 0.043	1.967SerGln: 1.967 ± 0.042	3.826SerArg: 3.826 ± 0.058	3.776SerSer: 3.776 ± 0.062	3.125SerThr: 3.125 ± 0.053	4.382SerVal: 4.382 ± 0.06	0.76SerTrp: 0.76 ± 0.02	1.398SerTyr: 1.398 ± 0.032	0.001SerXaa: 0.001 ± 0.001
Thr	6.069ThrAla: 6.069 ± 0.086	0.412ThrCys: 0.412 ± 0.017	2.807ThrAsp: 2.807 ± 0.044	2.794ThrGlu: 2.794 ± 0.04	2.065ThrPhe: 2.065 ± 0.039	5.04ThrGly: 5.04 ± 0.057	1.016ThrHis: 1.016 ± 0.025	3.244ThrIle: 3.244 ± 0.053	1.8ThrLys: 1.8 ± 0.039	5.62ThrLeu: 5.62 ± 0.055	1.328ThrMet: 1.328 ± 0.034	1.481ThrAsn: 1.481 ± 0.036	2.965ThrPro: 2.965 ± 0.046	1.573ThrGln: 1.573 ± 0.035	3.109ThrArg: 3.109 ± 0.048	3.18ThrSer: 3.18 ± 0.052	2.948ThrThr: 2.948 ± 0.053	4.339ThrVal: 4.339 ± 0.063	0.651ThrTrp: 0.651 ± 0.019	1.27ThrTyr: 1.27 ± 0.03	0.001ThrXaa: 0.001 ± 0.001
Val	8.274ValAla: 8.274 ± 0.089	0.609ValCys: 0.609 ± 0.021	4.076ValAsp: 4.076 ± 0.052	4.483ValGlu: 4.483 ± 0.046	2.771ValPhe: 2.771 ± 0.047	5.224ValGly: 5.224 ± 0.061	1.364ValHis: 1.364 ± 0.03	4.233ValIle: 4.233 ± 0.054	2.625ValLys: 2.625 ± 0.046	7.293ValLeu: 7.293 ± 0.065	1.907ValMet: 1.907 ± 0.04	2.143ValAsn: 2.143 ± 0.036	3.477ValPro: 3.477 ± 0.039	2.223ValGln: 2.223 ± 0.041	4.596ValArg: 4.596 ± 0.06	4.901ValSer: 4.901 ± 0.066	4.469ValThr: 4.469 ± 0.067	5.552ValVal: 5.552 ± 0.069	0.855ValTrp: 0.855 ± 0.026	1.46ValTyr: 1.46 ± 0.036	0.001ValXaa: 0.001 ± 0.001
Trp	1.075TrpAla: 1.075 ± 0.031	0.121TrpCys: 0.121 ± 0.008	0.576TrpAsp: 0.576 ± 0.018	0.551TrpGlu: 0.551 ± 0.018	0.518TrpPhe: 0.518 ± 0.022	0.779TrpGly: 0.779 ± 0.023	0.317TrpHis: 0.317 ± 0.013	0.665TrpIle: 0.665 ± 0.021	0.568TrpLys: 0.568 ± 0.018	1.567TrpLeu: 1.567 ± 0.035	0.37TrpMet: 0.37 ± 0.015	0.477TrpAsn: 0.477 ± 0.015	0.64TrpPro: 0.64 ± 0.022	0.594TrpGln: 0.594 ± 0.02	0.966TrpArg: 0.966 ± 0.025	0.822TrpSer: 0.822 ± 0.021	0.702TrpThr: 0.702 ± 0.023	0.711TrpVal: 0.711 ± 0.022	0.205TrpTrp: 0.205 ± 0.011	0.281TrpTyr: 0.281 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.416TyrAla: 2.416 ± 0.04	0.23TyrCys: 0.23 ± 0.011	1.393TyrAsp: 1.393 ± 0.032	1.243TyrGlu: 1.243 ± 0.024	0.961TyrPhe: 0.961 ± 0.025	2.055TyrGly: 2.055 ± 0.037	0.463TyrHis: 0.463 ± 0.015	0.972TyrIle: 0.972 ± 0.021	0.707TyrLys: 0.707 ± 0.027	2.373TyrLeu: 2.373 ± 0.046	0.453TyrMet: 0.453 ± 0.017	0.653TyrAsn: 0.653 ± 0.02	1.083TyrPro: 1.083 ± 0.026	0.789TyrGln: 0.789 ± 0.02	1.708TyrArg: 1.708 ± 0.039	1.306TyrSer: 1.306 ± 0.029	1.106TyrThr: 1.106 ± 0.027	1.617TyrVal: 1.617 ± 0.031	0.357TyrTrp: 0.357 ± 0.017	0.596TyrTyr: 0.596 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 5002 proteins (1581867 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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