Pseudobacteriovorax antillogorgiicola
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6344 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y6BZD0|A0A1Y6BZD0_9PROT Uncharacterized protein OS=Pseudobacteriovorax antillogorgiicola OX=1513793 GN=SAMN06296036_10912 PE=4 SV=1
MM1 pKa = 7.98 RR2 pKa = 11.84 YY3 pKa = 9.21 VLCSTLLLSIAASSCGEE20 pKa = 4.04 SDD22 pKa = 4.6 SEE24 pKa = 4.4 TSADD28 pKa = 3.43 NQTAVSVEE36 pKa = 4.05 FAAVAGTEE44 pKa = 4.32 SISCEE49 pKa = 3.82 TSLTGLGSNQDD60 pKa = 3.53 SATLADD66 pKa = 3.99 FRR68 pKa = 11.84 FYY70 pKa = 10.72 IHH72 pKa = 7.67 DD73 pKa = 3.63 IVFTKK78 pKa = 10.74 DD79 pKa = 3.18 DD80 pKa = 4.0 GSSISLTLDD89 pKa = 3.11 EE90 pKa = 5.92 NDD92 pKa = 3.34 WQTDD96 pKa = 3.32 NLALLDD102 pKa = 4.18 FQDD105 pKa = 5.23 KK106 pKa = 10.94 SDD108 pKa = 3.79 SCSGDD113 pKa = 3.05 EE114 pKa = 4.4 KK115 pKa = 10.59 DD116 pKa = 3.3 THH118 pKa = 7.87 KK119 pKa = 10.84 EE120 pKa = 3.76 VTGTVSDD127 pKa = 3.59 TTGISGISFTLGVPEE142 pKa = 5.21 DD143 pKa = 5.04 LNHH146 pKa = 6.43 QDD148 pKa = 3.16 QSSASTPLNIVSLFWSWNSGYY169 pKa = 10.8 KK170 pKa = 9.93 FLRR173 pKa = 11.84 ADD175 pKa = 3.45 VTPSNGITRR184 pKa = 11.84 PSDD187 pKa = 3.23 STFSGTTFNIHH198 pKa = 6.53 IGSTACTGDD207 pKa = 3.0 ATAGEE212 pKa = 5.22 DD213 pKa = 3.86 VTCTNSNRR221 pKa = 11.84 AQIALSDD228 pKa = 4.13 FEE230 pKa = 5.88 LGTSLVTFDD239 pKa = 4.05 YY240 pKa = 8.92 ATLVQSHH247 pKa = 7.27 NITSDD252 pKa = 2.96 VGGAPGCMSGSTDD265 pKa = 3.83 PEE267 pKa = 4.28 CQGVFSGLGLDD278 pKa = 4.61 LSSGTQSTTTQTAFSLQQ295 pKa = 3.31
Molecular weight: 30.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.935
Patrickios 1.189
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A1Y6BUH0|A0A1Y6BUH0_9PROT Histidine kinase OS=Pseudobacteriovorax antillogorgiicola OX=1513793 GN=SAMN06296036_109102 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 9.35 ATKK5 pKa = 10.26 KK6 pKa = 9.29 KK7 pKa = 6.81 TAKK10 pKa = 9.96 RR11 pKa = 11.84 AAAKK15 pKa = 10.37 KK16 pKa = 8.71 KK17 pKa = 5.15 TTKK20 pKa = 10.51 KK21 pKa = 10.22 KK22 pKa = 9.23 VAKK25 pKa = 8.91 KK26 pKa = 6.59 TTAKK30 pKa = 10.41 KK31 pKa = 9.96 KK32 pKa = 8.75 ATRR35 pKa = 11.84 KK36 pKa = 9.36 KK37 pKa = 9.93 AAKK40 pKa = 8.44 KK41 pKa = 6.31 TTAKK45 pKa = 10.35 KK46 pKa = 9.84 KK47 pKa = 8.89 ATRR50 pKa = 11.84 KK51 pKa = 8.33 STAKK55 pKa = 10.46 KK56 pKa = 10.01 KK57 pKa = 9.06 VAKK60 pKa = 10.34 KK61 pKa = 10.09 KK62 pKa = 8.6 ATKK65 pKa = 10.31 KK66 pKa = 9.99 KK67 pKa = 8.93 VARR70 pKa = 11.84 KK71 pKa = 9.23 KK72 pKa = 8.76 ATKK75 pKa = 10.3 KK76 pKa = 10.21 KK77 pKa = 8.71 VAKK80 pKa = 10.37 KK81 pKa = 10.04 KK82 pKa = 8.74 VAKK85 pKa = 10.47 KK86 pKa = 8.99 KK87 pKa = 5.33 TTKK90 pKa = 10.56 KK91 pKa = 10.32 KK92 pKa = 9.01 VAKK95 pKa = 10.31 KK96 pKa = 10.05 KK97 pKa = 8.6 ATKK100 pKa = 10.36 KK101 pKa = 10.29 KK102 pKa = 8.68 VAKK105 pKa = 10.4 KK106 pKa = 8.56 KK107 pKa = 5.57 TTRR110 pKa = 11.84 KK111 pKa = 9.75 KK112 pKa = 9.36 VAKK115 pKa = 10.01 KK116 pKa = 9.94 KK117 pKa = 8.55 AAKK120 pKa = 9.99 RR121 pKa = 11.84 ATTKK125 pKa = 10.66 RR126 pKa = 11.84 KK127 pKa = 4.17 TTRR130 pKa = 11.84 RR131 pKa = 11.84 KK132 pKa = 10.11 ASASAA137 pKa = 3.26
Molecular weight: 15.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.438
IPC2_protein 11.403
IPC_protein 12.749
Toseland 12.998
ProMoST 13.408
Dawson 13.013
Bjellqvist 12.939
Wikipedia 13.422
Rodwell 13.364
Grimsley 13.042
Solomon 13.437
Lehninger 13.349
Nozaki 12.998
DTASelect 12.939
Thurlkill 12.998
EMBOSS 13.481
Sillero 12.998
Patrickios 13.071
IPC_peptide 13.451
IPC2_peptide 12.398
IPC2.peptide.svr19 9.04
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6344
0
6344
2143815
25
3542
337.9
37.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.281 ± 0.028
1.12 ± 0.012
6.006 ± 0.025
6.503 ± 0.026
4.556 ± 0.021
6.812 ± 0.029
2.168 ± 0.016
6.424 ± 0.025
5.842 ± 0.034
10.271 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.217 ± 0.013
3.97 ± 0.022
4.056 ± 0.019
4.335 ± 0.023
5.185 ± 0.021
7.719 ± 0.028
4.842 ± 0.019
6.274 ± 0.022
1.23 ± 0.011
3.188 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here