Streptococcus phage Javan288

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AZQ2|A0A4D6AZQ2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan288 OX=2548094 GN=Javan288_0043 PE=4 SV=1
MM1 pKa = 7.45SVTKK5 pKa = 10.46EE6 pKa = 3.98EE7 pKa = 4.23VMLALNLDD15 pKa = 3.72EE16 pKa = 6.11SDD18 pKa = 4.48DD19 pKa = 3.83VALIPAYY26 pKa = 8.86ITTAEE31 pKa = 4.17IYY33 pKa = 10.26IKK35 pKa = 10.74NAVGNADD42 pKa = 3.46GFFEE46 pKa = 4.25QEE48 pKa = 4.22HH49 pKa = 6.03VEE51 pKa = 4.09PLYY54 pKa = 10.49DD55 pKa = 3.61TAVLALASSYY65 pKa = 8.38YY66 pKa = 8.68TYY68 pKa = 10.97RR69 pKa = 11.84VALTDD74 pKa = 3.23AMTYY78 pKa = 10.34PIDD81 pKa = 3.6LTLNSVIGQLRR92 pKa = 11.84GLYY95 pKa = 9.95AVYY98 pKa = 10.77CEE100 pKa = 4.36GG101 pKa = 4.24

Molecular weight: 11.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B131|A0A4D6B131_9CAUD Uncharacterized protein OS=Streptococcus phage Javan288 OX=2548094 GN=Javan288_0044 PE=4 SV=1
MM1 pKa = 7.53ARR3 pKa = 11.84KK4 pKa = 9.13QYY6 pKa = 10.95KK7 pKa = 8.28PTDD10 pKa = 3.85FRR12 pKa = 11.84NKK14 pKa = 10.66AEE16 pKa = 4.15FGTYY20 pKa = 9.47EE21 pKa = 4.28SIPNKK26 pKa = 8.97FTGVSVPKK34 pKa = 10.19FVSKK38 pKa = 10.46FILHH42 pKa = 6.13YY43 pKa = 10.21KK44 pKa = 8.65PHH46 pKa = 6.25TRR48 pKa = 11.84TLNQQYY54 pKa = 10.41LAISAGEE61 pKa = 4.14SEE63 pKa = 4.13SRR65 pKa = 11.84IIVIRR70 pKa = 11.84HH71 pKa = 4.61NPKK74 pKa = 9.66VVEE77 pKa = 4.1GLAVRR82 pKa = 11.84LNGTVYY88 pKa = 10.63NVDD91 pKa = 4.14KK92 pKa = 10.87ISPDD96 pKa = 3.24EE97 pKa = 3.97NFGLNRR103 pKa = 11.84YY104 pKa = 10.01DD105 pKa = 4.37FVTLKK110 pKa = 10.66KK111 pKa = 10.06SEE113 pKa = 4.23KK114 pKa = 10.04VGKK117 pKa = 10.23QNGG120 pKa = 3.37

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11660

43

1582

228.6

25.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.007 ± 0.485

0.583 ± 0.138

6.561 ± 0.317

6.878 ± 0.563

4.091 ± 0.175

6.664 ± 0.513

1.346 ± 0.112

6.527 ± 0.268

8.576 ± 0.477

7.753 ± 0.347

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.175

6.278 ± 0.291

2.762 ± 0.173

4.057 ± 0.186

3.816 ± 0.35

6.364 ± 0.444

6.398 ± 0.536

6.244 ± 0.28

1.407 ± 0.107

4.005 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski