candidate division Zixibacteria bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; candidate division Zixibacteria; unclassified candidate division Zixibacteria

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3031 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I7LZI6|A0A6I7LZI6_9BACT Uncharacterized protein OS=candidate division Zixibacteria bacterium OX=2053527 GN=TRIP_C21254 PE=4 SV=1
MM1 pKa = 7.46FKK3 pKa = 9.81TAFLLTAILSLFGPLKK19 pKa = 10.46VYY21 pKa = 10.7SFEE24 pKa = 5.18PIFNARR30 pKa = 11.84IDD32 pKa = 3.96YY33 pKa = 10.1EE34 pKa = 4.46VGSNPQAICTADD46 pKa = 3.48LNGDD50 pKa = 4.05GKK52 pKa = 11.18ADD54 pKa = 3.7LAVANLEE61 pKa = 4.08SHH63 pKa = 6.93NISILINQGDD73 pKa = 4.12GTFTSAINYY82 pKa = 8.1AAGLYY87 pKa = 7.84PASICTADD95 pKa = 3.41FDD97 pKa = 5.59GNGTADD103 pKa = 3.92LAVTHH108 pKa = 6.8AMDD111 pKa = 4.62SVVILINNGGGSSFMKK127 pKa = 6.85TTYY130 pKa = 10.24YY131 pKa = 10.89AGGVPYY137 pKa = 10.24SIYY140 pKa = 10.92AADD143 pKa = 3.8IDD145 pKa = 4.59GDD147 pKa = 4.07GDD149 pKa = 3.88TDD151 pKa = 4.21LATANYY157 pKa = 9.21VSNSVSILINNGDD170 pKa = 3.92GTFSSPISYY179 pKa = 10.3VVGGDD184 pKa = 2.9AWSICAADD192 pKa = 4.69LDD194 pKa = 4.74ADD196 pKa = 4.05GTTDD200 pKa = 4.2LALAIDD206 pKa = 4.08KK207 pKa = 10.5GVSVLRR213 pKa = 11.84NNGDD217 pKa = 3.71GTFSAPNIYY226 pKa = 10.09EE227 pKa = 3.96VGFTSIFICAVDD239 pKa = 3.84VNATGIIDD247 pKa = 3.97LAVVNHH253 pKa = 6.48NGVTVLKK260 pKa = 10.52NKK262 pKa = 10.36GDD264 pKa = 3.88GTFGALDD271 pKa = 3.74YY272 pKa = 11.24YY273 pKa = 10.37EE274 pKa = 4.86TGSDD278 pKa = 3.26PWSVIAADD286 pKa = 4.02LDD288 pKa = 4.03GDD290 pKa = 4.28GFSDD294 pKa = 4.62LAISHH299 pKa = 5.68IRR301 pKa = 11.84FWGRR305 pKa = 11.84ISILKK310 pKa = 10.31NNGDD314 pKa = 3.78GTFAAAVSFDD324 pKa = 3.98AKK326 pKa = 9.87EE327 pKa = 3.79KK328 pKa = 10.58SYY330 pKa = 11.04SVCAADD336 pKa = 4.32FNGDD340 pKa = 3.51NRR342 pKa = 11.84PDD344 pKa = 3.35LAVANGLWNSISILRR359 pKa = 11.84NGGNAVFPVAPYY371 pKa = 9.78FWVGDD376 pKa = 4.18LPSSAYY382 pKa = 9.63PADD385 pKa = 3.65FDD387 pKa = 4.92GDD389 pKa = 3.99GKK391 pKa = 10.7IDD393 pKa = 4.15LAVLNAGEE401 pKa = 4.05YY402 pKa = 9.76PYY404 pKa = 10.28TEE406 pKa = 3.59RR407 pKa = 11.84VSILKK412 pKa = 10.63NNGNTTFSSADD423 pKa = 3.59SYY425 pKa = 11.87SMEE428 pKa = 4.17HH429 pKa = 6.01FAKK432 pKa = 10.18SVYY435 pKa = 10.55AADD438 pKa = 3.74FDD440 pKa = 5.08GDD442 pKa = 3.77GKK444 pKa = 11.2ADD446 pKa = 3.66LVAANDD452 pKa = 4.31DD453 pKa = 4.01DD454 pKa = 5.19PGTVSLFKK462 pKa = 11.27NNGDD466 pKa = 3.61GTFAAAVNYY475 pKa = 9.64GAGALPYY482 pKa = 9.35NICAADD488 pKa = 3.53FDD490 pKa = 5.3GDD492 pKa = 3.73GSADD496 pKa = 3.33LAVANRR502 pKa = 11.84GNDD505 pKa = 3.12ANYY508 pKa = 10.0PGGFSILKK516 pKa = 10.09NNGDD520 pKa = 3.78GTFAPAVSYY529 pKa = 10.65GAGGGTSICSADD541 pKa = 3.21FDD543 pKa = 5.34GNGKK547 pKa = 10.07ADD549 pKa = 4.0LALSNAGGVSILINNGDD566 pKa = 3.74GTFAAAVNCNTGISPSSIVAVDD588 pKa = 3.79CDD590 pKa = 3.91SDD592 pKa = 3.82GDD594 pKa = 3.92ADD596 pKa = 4.09LAVANRR602 pKa = 11.84GSGSDD607 pKa = 3.61YY608 pKa = 10.51PGNISILINNGDD620 pKa = 3.74GTFAAAVNYY629 pKa = 10.71AEE631 pKa = 4.49GVRR634 pKa = 11.84PSALMVADD642 pKa = 4.69FSGDD646 pKa = 3.61GIADD650 pKa = 3.89LAVKK654 pKa = 10.09NNCDD658 pKa = 3.67VLILICLGDD667 pKa = 3.72GTFVSTLNYY676 pKa = 9.87GVRR679 pKa = 11.84DD680 pKa = 4.05DD681 pKa = 4.39GFGSISAADD690 pKa = 3.95FNNDD694 pKa = 3.28GKK696 pKa = 10.15PDD698 pKa = 3.75LAVTTGGRR706 pKa = 11.84SDD708 pKa = 3.33VQFLFNIAAFVFVCGDD724 pKa = 3.45ADD726 pKa = 3.81RR727 pKa = 11.84SGTAGISDD735 pKa = 3.52ITYY738 pKa = 10.71VIDD741 pKa = 3.59FLYY744 pKa = 10.62RR745 pKa = 11.84FGSAPIPVQAADD757 pKa = 3.62INNSGTVNILDD768 pKa = 3.41ISYY771 pKa = 9.05MINYY775 pKa = 9.34LYY777 pKa = 11.2KK778 pKa = 10.74NGTALNCPP786 pKa = 4.03

Molecular weight: 81.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I7LPB5|A0A6I7LPB5_9BACT Putative Transcriptional regulator TetR family OS=candidate division Zixibacteria bacterium OX=2053527 GN=TRIP_C20142 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 9.0KK12 pKa = 11.08LNDD15 pKa = 3.15HH16 pKa = 6.3GFRR19 pKa = 11.84KK20 pKa = 10.1RR21 pKa = 11.84MATADD26 pKa = 3.39GRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.35RR41 pKa = 11.84LTVSVRR47 pKa = 11.84KK48 pKa = 9.93RR49 pKa = 11.84KK50 pKa = 9.58

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3031

0

3031

1045971

20

2865

345.1

38.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.147 ± 0.041

1.003 ± 0.021

5.702 ± 0.037

5.79 ± 0.05

4.637 ± 0.035

7.701 ± 0.042

1.8 ± 0.022

7.641 ± 0.033

5.606 ± 0.046

9.574 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.02

4.304 ± 0.034

4.409 ± 0.031

2.92 ± 0.024

5.376 ± 0.043

6.467 ± 0.04

5.194 ± 0.041

6.349 ± 0.039

1.134 ± 0.017

3.779 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski