Amino acid dipepetide frequency for candidate division Zixibacteria bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.355AlaAla: 8.355 ± 0.127	0.806AlaCys: 0.806 ± 0.033	4.994AlaAsp: 4.994 ± 0.076	5.209AlaGlu: 5.209 ± 0.095	3.318AlaPhe: 3.318 ± 0.053	7.277AlaGly: 7.277 ± 0.091	1.298AlaHis: 1.298 ± 0.039	5.818AlaIle: 5.818 ± 0.077	3.698AlaLys: 3.698 ± 0.07	7.849AlaLeu: 7.849 ± 0.119	1.969AlaMet: 1.969 ± 0.049	2.851AlaAsn: 2.851 ± 0.054	3.011AlaPro: 3.011 ± 0.064	2.285AlaGln: 2.285 ± 0.05	4.412AlaArg: 4.412 ± 0.075	4.644AlaSer: 4.644 ± 0.084	3.805AlaThr: 3.805 ± 0.07	6.578AlaVal: 6.578 ± 0.085	0.737AlaTrp: 0.737 ± 0.031	2.547AlaTyr: 2.547 ± 0.05	0.005AlaXaa: 0.005 ± 0.002
Cys	0.684CysAla: 0.684 ± 0.03	0.145CysCys: 0.145 ± 0.012	0.598CysAsp: 0.598 ± 0.032	0.446CysGlu: 0.446 ± 0.02	0.428CysPhe: 0.428 ± 0.034	1.133CysGly: 1.133 ± 0.04	0.492CysHis: 0.492 ± 0.049	0.488CysIle: 0.488 ± 0.023	0.357CysLys: 0.357 ± 0.019	0.85CysLeu: 0.85 ± 0.031	0.166CysMet: 0.166 ± 0.015	0.4CysAsn: 0.4 ± 0.019	0.718CysPro: 0.718 ± 0.034	0.346CysGln: 0.346 ± 0.018	0.739CysArg: 0.739 ± 0.033	0.637CysSer: 0.637 ± 0.028	0.446CysThr: 0.446 ± 0.023	0.451CysVal: 0.451 ± 0.021	0.122CysTrp: 0.122 ± 0.012	0.383CysTyr: 0.383 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.708AspAla: 3.708 ± 0.062	0.631AspCys: 0.631 ± 0.027	2.989AspAsp: 2.989 ± 0.057	3.158AspGlu: 3.158 ± 0.064	2.906AspPhe: 2.906 ± 0.055	4.438AspGly: 4.438 ± 0.097	0.911AspHis: 0.911 ± 0.03	4.636AspIle: 4.636 ± 0.072	3.141AspLys: 3.141 ± 0.058	5.292AspLeu: 5.292 ± 0.068	1.289AspMet: 1.289 ± 0.035	2.675AspAsn: 2.675 ± 0.056	2.641AspPro: 2.641 ± 0.056	1.51AspGln: 1.51 ± 0.037	3.135AspArg: 3.135 ± 0.065	4.644AspSer: 4.644 ± 0.099	3.09AspThr: 3.09 ± 0.084	3.013AspVal: 3.013 ± 0.062	0.755AspTrp: 0.755 ± 0.027	2.461AspTyr: 2.461 ± 0.059	0.004AspXaa: 0.004 ± 0.002
Glu	4.662GluAla: 4.662 ± 0.086	0.462GluCys: 0.462 ± 0.024	2.366GluAsp: 2.366 ± 0.054	3.738GluGlu: 3.738 ± 0.073	2.469GluPhe: 2.469 ± 0.052	3.504GluGly: 3.504 ± 0.06	0.919GluHis: 0.919 ± 0.035	5.388GluIle: 5.388 ± 0.096	4.957GluLys: 4.957 ± 0.096	5.138GluLeu: 5.138 ± 0.079	1.891GluMet: 1.891 ± 0.049	2.714GluAsn: 2.714 ± 0.052	2.001GluPro: 2.001 ± 0.041	1.795GluGln: 1.795 ± 0.054	3.271GluArg: 3.271 ± 0.057	3.45GluSer: 3.45 ± 0.064	2.967GluThr: 2.967 ± 0.057	3.503GluVal: 3.503 ± 0.07	0.567GluTrp: 0.567 ± 0.02	2.14GluTyr: 2.14 ± 0.045	0.002GluXaa: 0.002 ± 0.001
Phe	3.573PheAla: 3.573 ± 0.06	0.543PheCys: 0.543 ± 0.027	3.001PheAsp: 3.001 ± 0.057	2.495PheGlu: 2.495 ± 0.048	2.125PhePhe: 2.125 ± 0.057	3.813PheGly: 3.813 ± 0.067	0.892PheHis: 0.892 ± 0.029	3.143PheIle: 3.143 ± 0.064	2.17PheLys: 2.17 ± 0.047	4.636PheLeu: 4.636 ± 0.093	1.054PheMet: 1.054 ± 0.033	2.067PheAsn: 2.067 ± 0.05	1.957PhePro: 1.957 ± 0.045	1.312PheGln: 1.312 ± 0.037	2.643PheArg: 2.643 ± 0.053	3.404PheSer: 3.404 ± 0.066	2.215PheThr: 2.215 ± 0.05	2.817PheVal: 2.817 ± 0.054	0.639PheTrp: 0.639 ± 0.026	1.869PheTyr: 1.869 ± 0.044	0.005PheXaa: 0.005 ± 0.002
Gly	5.812GlyAla: 5.812 ± 0.087	0.868GlyCys: 0.868 ± 0.034	4.251GlyAsp: 4.251 ± 0.084	4.303GlyGlu: 4.303 ± 0.069	3.484GlyPhe: 3.484 ± 0.07	6.127GlyGly: 6.127 ± 0.103	1.553GlyHis: 1.553 ± 0.04	5.802GlyIle: 5.802 ± 0.08	4.702GlyLys: 4.702 ± 0.076	7.184GlyLeu: 7.184 ± 0.101	2.02GlyMet: 2.02 ± 0.048	3.523GlyAsn: 3.523 ± 0.075	2.559GlyPro: 2.559 ± 0.051	2.523GlyGln: 2.523 ± 0.051	4.293GlyArg: 4.293 ± 0.067	4.645GlySer: 4.645 ± 0.083	4.573GlyThr: 4.573 ± 0.088	4.704GlyVal: 4.704 ± 0.069	1.022GlyTrp: 1.022 ± 0.033	3.052GlyTyr: 3.052 ± 0.062	0.011GlyXaa: 0.011 ± 0.004
His	1.142HisAla: 1.142 ± 0.031	0.25HisCys: 0.25 ± 0.015	1.015HisAsp: 1.015 ± 0.037	0.931HisGlu: 0.931 ± 0.033	1.042HisPhe: 1.042 ± 0.035	1.318HisGly: 1.318 ± 0.047	0.432HisHis: 0.432 ± 0.018	1.325HisIle: 1.325 ± 0.037	0.866HisLys: 0.866 ± 0.031	1.999HisLeu: 1.999 ± 0.045	0.379HisMet: 0.379 ± 0.017	0.764HisAsn: 0.764 ± 0.028	1.077HisPro: 1.077 ± 0.031	0.614HisGln: 0.614 ± 0.025	1.041HisArg: 1.041 ± 0.034	1.124HisSer: 1.124 ± 0.036	0.832HisThr: 0.832 ± 0.029	0.802HisVal: 0.802 ± 0.028	0.253HisTrp: 0.253 ± 0.017	0.789HisTyr: 0.789 ± 0.028	0.003HisXaa: 0.003 ± 0.002
Ile	6.049IleAla: 6.049 ± 0.089	0.803IleCys: 0.803 ± 0.041	5.035IleAsp: 5.035 ± 0.075	4.212IleGlu: 4.212 ± 0.067	3.87IlePhe: 3.87 ± 0.074	5.525IleGly: 5.525 ± 0.078	1.199IleHis: 1.199 ± 0.037	5.896IleIle: 5.896 ± 0.099	4.153IleLys: 4.153 ± 0.066	7.476IleLeu: 7.476 ± 0.1	1.74IleMet: 1.74 ± 0.041	3.436IleAsn: 3.436 ± 0.063	3.561IlePro: 3.561 ± 0.059	1.802IleGln: 1.802 ± 0.042	3.845IleArg: 3.845 ± 0.066	5.403IleSer: 5.403 ± 0.075	3.956IleThr: 3.956 ± 0.074	4.598IleVal: 4.598 ± 0.074	0.846IleTrp: 0.846 ± 0.03	3.001IleTyr: 3.001 ± 0.063	0.005IleXaa: 0.005 ± 0.002
Lys	4.203LysAla: 4.203 ± 0.078	0.48LysCys: 0.48 ± 0.022	2.87LysAsp: 2.87 ± 0.06	3.847LysGlu: 3.847 ± 0.08	2.321LysPhe: 2.321 ± 0.048	3.682LysGly: 3.682 ± 0.06	0.836LysHis: 0.836 ± 0.032	5.058LysIle: 5.058 ± 0.07	4.251LysLys: 4.251 ± 0.093	4.764LysLeu: 4.764 ± 0.078	1.892LysMet: 1.892 ± 0.049	2.558LysAsn: 2.558 ± 0.053	2.078LysPro: 2.078 ± 0.057	1.457LysGln: 1.457 ± 0.04	2.799LysArg: 2.799 ± 0.059	3.533LysSer: 3.533 ± 0.063	2.866LysThr: 2.866 ± 0.054	3.584LysVal: 3.584 ± 0.065	0.585LysTrp: 0.585 ± 0.026	2.391LysTyr: 2.391 ± 0.046	0.007LysXaa: 0.007 ± 0.002
Leu	8.878LeuAla: 8.878 ± 0.118	0.84LeuCys: 0.84 ± 0.034	5.139LeuAsp: 5.139 ± 0.069	5.417LeuGlu: 5.417 ± 0.085	4.537LeuPhe: 4.537 ± 0.087	6.866LeuGly: 6.866 ± 0.1	1.603LeuHis: 1.603 ± 0.039	7.175LeuIle: 7.175 ± 0.101	5.951LeuLys: 5.951 ± 0.09	9.154LeuLeu: 9.154 ± 0.127	2.274LeuMet: 2.274 ± 0.047	4.169LeuAsn: 4.169 ± 0.064	4.378LeuPro: 4.378 ± 0.069	2.534LeuGln: 2.534 ± 0.05	4.672LeuArg: 4.672 ± 0.076	6.627LeuSer: 6.627 ± 0.093	5.394LeuThr: 5.394 ± 0.073	5.825LeuVal: 5.825 ± 0.078	0.979LeuTrp: 0.979 ± 0.035	3.312LeuTyr: 3.312 ± 0.067	0.009LeuXaa: 0.009 ± 0.003
Met	2.585MetAla: 2.585 ± 0.049	0.154MetCys: 0.154 ± 0.013	1.073MetAsp: 1.073 ± 0.034	1.438MetGlu: 1.438 ± 0.035	0.804MetPhe: 0.804 ± 0.03	1.766MetGly: 1.766 ± 0.044	0.38MetHis: 0.38 ± 0.022	1.831MetIle: 1.831 ± 0.041	1.854MetLys: 1.854 ± 0.042	1.976MetLeu: 1.976 ± 0.047	0.726MetMet: 0.726 ± 0.032	1.063MetAsn: 1.063 ± 0.029	1.229MetPro: 1.229 ± 0.034	0.714MetGln: 0.714 ± 0.028	1.33MetArg: 1.33 ± 0.031	1.516MetSer: 1.516 ± 0.038	1.707MetThr: 1.707 ± 0.034	1.769MetVal: 1.769 ± 0.045	0.146MetTrp: 0.146 ± 0.011	0.526MetTyr: 0.526 ± 0.023	0.004MetXaa: 0.004 ± 0.002
Asn	2.666AsnAla: 2.666 ± 0.053	0.582AsnCys: 0.582 ± 0.028	2.425AsnAsp: 2.425 ± 0.051	1.941AsnGlu: 1.941 ± 0.049	2.217AsnPhe: 2.217 ± 0.048	3.218AsnGly: 3.218 ± 0.072	0.834AsnHis: 0.834 ± 0.027	3.784AsnIle: 3.784 ± 0.063	1.988AsnLys: 1.988 ± 0.053	4.692AsnLeu: 4.692 ± 0.074	1.024AsnMet: 1.024 ± 0.027	2.015AsnAsn: 2.015 ± 0.055	2.453AsnPro: 2.453 ± 0.059	1.443AsnGln: 1.443 ± 0.04	2.531AsnArg: 2.531 ± 0.045	2.539AsnSer: 2.539 ± 0.057	1.717AsnThr: 1.717 ± 0.047	2.273AsnVal: 2.273 ± 0.054	0.654AsnTrp: 0.654 ± 0.027	2.038AsnTyr: 2.038 ± 0.055	0.005AsnXaa: 0.005 ± 0.003
Pro	3.823ProAla: 3.823 ± 0.06	0.344ProCys: 0.344 ± 0.016	3.336ProAsp: 3.336 ± 0.067	3.38ProGlu: 3.38 ± 0.064	2.078ProPhe: 2.078 ± 0.051	3.693ProGly: 3.693 ± 0.072	0.831ProHis: 0.831 ± 0.031	2.791ProIle: 2.791 ± 0.052	1.946ProLys: 1.946 ± 0.042	3.834ProLeu: 3.834 ± 0.057	0.86ProMet: 0.86 ± 0.029	1.745ProAsn: 1.745 ± 0.044	2.126ProPro: 2.126 ± 0.057	1.308ProGln: 1.308 ± 0.034	1.871ProArg: 1.871 ± 0.043	2.786ProSer: 2.786 ± 0.056	2.088ProThr: 2.088 ± 0.054	3.309ProVal: 3.309 ± 0.06	0.408ProTrp: 0.408 ± 0.023	1.536ProTyr: 1.536 ± 0.044	0.002ProXaa: 0.002 ± 0.002
Gln	2.396GlnAla: 2.396 ± 0.056	0.241GlnCys: 0.241 ± 0.015	1.301GlnAsp: 1.301 ± 0.035	1.627GlnGlu: 1.627 ± 0.046	1.429GlnPhe: 1.429 ± 0.033	1.843GlnGly: 1.843 ± 0.04	0.436GlnHis: 0.436 ± 0.022	2.437GlnIle: 2.437 ± 0.049	1.972GlnLys: 1.972 ± 0.045	2.714GlnLeu: 2.714 ± 0.057	0.903GlnMet: 0.903 ± 0.032	1.454GlnAsn: 1.454 ± 0.035	1.158GlnPro: 1.158 ± 0.035	0.94GlnGln: 0.94 ± 0.032	1.354GlnArg: 1.354 ± 0.04	1.86GlnSer: 1.86 ± 0.048	1.576GlnThr: 1.576 ± 0.045	1.974GlnVal: 1.974 ± 0.047	0.348GlnTrp: 0.348 ± 0.02	1.236GlnTyr: 1.236 ± 0.037	0.001GlnXaa: 0.001 ± 0.001
Arg	4.011ArgAla: 4.011 ± 0.077	0.424ArgCys: 0.424 ± 0.022	2.81ArgAsp: 2.81 ± 0.05	3.51ArgGlu: 3.51 ± 0.073	2.612ArgPhe: 2.612 ± 0.052	3.305ArgGly: 3.305 ± 0.061	1.134ArgHis: 1.134 ± 0.033	4.152ArgIle: 4.152 ± 0.075	3.336ArgLys: 3.336 ± 0.061	5.757ArgLeu: 5.757 ± 0.095	1.408ArgMet: 1.408 ± 0.033	2.292ArgAsn: 2.292 ± 0.047	2.372ArgPro: 2.372 ± 0.053	2.022ArgGln: 2.022 ± 0.048	3.368ArgArg: 3.368 ± 0.077	2.824ArgSer: 2.824 ± 0.059	2.626ArgThr: 2.626 ± 0.049	3.149ArgVal: 3.149 ± 0.057	0.573ArgTrp: 0.573 ± 0.024	2.073ArgTyr: 2.073 ± 0.045	0.003ArgXaa: 0.003 ± 0.002
Ser	5.297SerAla: 5.297 ± 0.078	0.675SerCys: 0.675 ± 0.035	3.856SerAsp: 3.856 ± 0.063	3.453SerGlu: 3.453 ± 0.062	3.133SerPhe: 3.133 ± 0.058	6.486SerGly: 6.486 ± 0.096	1.187SerHis: 1.187 ± 0.034	4.487SerIle: 4.487 ± 0.077	2.731SerLys: 2.731 ± 0.059	6.618SerLeu: 6.618 ± 0.092	1.33SerMet: 1.33 ± 0.034	2.393SerAsn: 2.393 ± 0.051	2.955SerPro: 2.955 ± 0.051	2.001SerGln: 2.001 ± 0.047	3.653SerArg: 3.653 ± 0.067	4.209SerSer: 4.209 ± 0.07	3.147SerThr: 3.147 ± 0.068	3.951SerVal: 3.951 ± 0.072	0.763SerTrp: 0.763 ± 0.03	2.34SerTyr: 2.34 ± 0.052	0.002SerXaa: 0.002 ± 0.001
Thr	5.0ThrAla: 5.0 ± 0.073	0.541ThrCys: 0.541 ± 0.028	3.112ThrAsp: 3.112 ± 0.062	2.769ThrGlu: 2.769 ± 0.051	2.371ThrPhe: 2.371 ± 0.054	4.835ThrGly: 4.835 ± 0.073	0.88ThrHis: 0.88 ± 0.033	3.954ThrIle: 3.954 ± 0.068	2.16ThrLys: 2.16 ± 0.054	4.862ThrLeu: 4.862 ± 0.072	1.086ThrMet: 1.086 ± 0.031	1.86ThrAsn: 1.86 ± 0.05	2.499ThrPro: 2.499 ± 0.064	1.251ThrGln: 1.251 ± 0.041	2.33ThrArg: 2.33 ± 0.046	3.146ThrSer: 3.146 ± 0.058	2.79ThrThr: 2.79 ± 0.058	4.249ThrVal: 4.249 ± 0.08	0.545ThrTrp: 0.545 ± 0.026	1.694ThrTyr: 1.694 ± 0.042	0.004ThrXaa: 0.004 ± 0.002
Val	5.48ValAla: 5.48 ± 0.08	0.708ValCys: 0.708 ± 0.033	3.658ValAsp: 3.658 ± 0.07	3.611ValGlu: 3.611 ± 0.066	2.587ValPhe: 2.587 ± 0.045	4.577ValGly: 4.577 ± 0.082	1.021ValHis: 1.021 ± 0.035	5.015ValIle: 5.015 ± 0.082	3.497ValLys: 3.497 ± 0.064	5.67ValLeu: 5.67 ± 0.071	1.528ValMet: 1.528 ± 0.043	2.719ValAsn: 2.719 ± 0.058	2.974ValPro: 2.974 ± 0.05	1.617ValGln: 1.617 ± 0.044	3.332ValArg: 3.332 ± 0.06	4.396ValSer: 4.396 ± 0.078	3.888ValThr: 3.888 ± 0.072	4.39ValVal: 4.39 ± 0.075	0.686ValTrp: 0.686 ± 0.026	2.135ValTyr: 2.135 ± 0.044	0.003ValXaa: 0.003 ± 0.002
Trp	0.709TrpAla: 0.709 ± 0.028	0.119TrpCys: 0.119 ± 0.012	0.685TrpAsp: 0.685 ± 0.028	0.605TrpGlu: 0.605 ± 0.022	0.478TrpPhe: 0.478 ± 0.023	0.884TrpGly: 0.884 ± 0.029	0.305TrpHis: 0.305 ± 0.018	0.729TrpIle: 0.729 ± 0.033	0.62TrpLys: 0.62 ± 0.024	1.079TrpLeu: 1.079 ± 0.041	0.302TrpMet: 0.302 ± 0.018	0.587TrpAsn: 0.587 ± 0.032	0.492TrpPro: 0.492 ± 0.024	0.426TrpGln: 0.426 ± 0.02	0.65TrpArg: 0.65 ± 0.025	0.772TrpSer: 0.772 ± 0.033	0.594TrpThr: 0.594 ± 0.028	0.714TrpVal: 0.714 ± 0.024	0.186TrpTrp: 0.186 ± 0.013	0.398TrpTyr: 0.398 ± 0.024	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.426TyrAla: 2.426 ± 0.052	0.412TyrCys: 0.412 ± 0.021	2.504TyrAsp: 2.504 ± 0.075	1.812TyrGlu: 1.812 ± 0.04	2.153TyrPhe: 2.153 ± 0.049	2.711TyrGly: 2.711 ± 0.059	0.855TyrHis: 0.855 ± 0.031	2.496TyrIle: 2.496 ± 0.053	1.709TyrLys: 1.709 ± 0.041	4.187TyrLeu: 4.187 ± 0.073	0.747TyrMet: 0.747 ± 0.03	1.75TyrAsn: 1.75 ± 0.036	1.851TyrPro: 1.851 ± 0.044	1.299TyrGln: 1.299 ± 0.037	2.487TyrArg: 2.487 ± 0.046	2.505TyrSer: 2.505 ± 0.065	1.658TyrThr: 1.658 ± 0.045	1.839TyrVal: 1.839 ± 0.04	0.519TyrTrp: 0.519 ± 0.024	1.863TyrTyr: 1.863 ± 0.066	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.008XaaAla: 0.008 ± 0.003	0.001XaaCys: 0.001 ± 0.001	0.008XaaAsp: 0.008 ± 0.003	0.001XaaGlu: 0.001 ± 0.001	0.01XaaPhe: 0.01 ± 0.003	0.009XaaGly: 0.009 ± 0.003	0.003XaaHis: 0.003 ± 0.002	0.006XaaIle: 0.006 ± 0.002	0.004XaaLys: 0.004 ± 0.002	0.005XaaLeu: 0.005 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.003XaaAsn: 0.003 ± 0.002	0.004XaaPro: 0.004 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.004XaaArg: 0.004 ± 0.002	0.003XaaSer: 0.003 ± 0.002	0.003XaaThr: 0.003 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.002XaaTyr: 0.002 ± 0.001	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 3031 proteins (1045971 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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