Clostridium phage phiSM101

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0SPH5|Q0SPH5_9CAUD Uncharacterized protein OS=Clostridium phage phiSM101 OX=396359 GN=CPR_C0042 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.68VKK4 pKa = 9.09ITFIKK9 pKa = 9.88TSIDD13 pKa = 3.37KK14 pKa = 11.03LVDD17 pKa = 3.25LWIEE21 pKa = 4.11EE22 pKa = 4.37EE23 pKa = 4.24EE24 pKa = 4.9LEE26 pKa = 4.49QLKK29 pKa = 10.67NDD31 pKa = 4.07SFVKK35 pKa = 10.67SIDD38 pKa = 3.91RR39 pKa = 11.84NWDD42 pKa = 3.25DD43 pKa = 5.37SKK45 pKa = 11.4EE46 pKa = 3.79AWLYY50 pKa = 10.52SIEE53 pKa = 4.11YY54 pKa = 10.04QNGDD58 pKa = 3.56SEE60 pKa = 4.87EE61 pKa = 4.59VYY63 pKa = 11.22CNVV66 pKa = 3.53

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0SPI9|Q0SPI9_9CAUD Uncharacterized protein OS=Clostridium phage phiSM101 OX=396359 GN=CPR_C0028 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 9.67MSKK5 pKa = 10.6EE6 pKa = 3.98EE7 pKa = 3.91FVRR10 pKa = 11.84RR11 pKa = 11.84ASKK14 pKa = 10.61EE15 pKa = 3.65MAKK18 pKa = 10.15KK19 pKa = 9.44KK20 pKa = 10.0RR21 pKa = 11.84KK22 pKa = 9.68RR23 pKa = 11.84EE24 pKa = 3.75MLEE27 pKa = 3.9YY28 pKa = 10.4DD29 pKa = 3.4SKK31 pKa = 11.31SYY33 pKa = 10.68KK34 pKa = 10.54FNVLGKK40 pKa = 10.4ARR42 pKa = 11.84AKK44 pKa = 10.46LMKK47 pKa = 10.22KK48 pKa = 9.97KK49 pKa = 10.69FYY51 pKa = 10.75RR52 pKa = 4.02

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

8882

39

1178

167.6

19.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.607 ± 0.847

1.171 ± 0.209

5.539 ± 0.27

9.851 ± 0.515

4.064 ± 0.301

5.359 ± 0.4

1.025 ± 0.124

7.892 ± 0.482

11.833 ± 0.406

8.883 ± 0.42

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.225

6.947 ± 0.451

1.801 ± 0.218

2.837 ± 0.229

3.941 ± 0.272

5.787 ± 0.363

4.774 ± 0.438

5.652 ± 0.256

0.968 ± 0.144

3.58 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski