Amino acid dipepetide frequency for Clostridium phage phiSM101

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.617AlaAla: 4.617 ± 1.872
0.338AlaCys: 0.338 ± 0.186
3.378AlaAsp: 3.378 ± 0.506
4.504AlaGlu: 4.504 ± 1.065
1.464AlaPhe: 1.464 ± 0.418
4.729AlaGly: 4.729 ± 0.98
0.788AlaHis: 0.788 ± 0.302
4.617AlaIle: 4.617 ± 0.692
7.432AlaLys: 7.432 ± 1.238
3.265AlaLeu: 3.265 ± 0.633
1.576AlaMet: 1.576 ± 0.484
2.702AlaAsn: 2.702 ± 0.57
1.013AlaPro: 1.013 ± 0.335
1.576AlaGln: 1.576 ± 0.383
1.689AlaArg: 1.689 ± 0.455
3.491AlaSer: 3.491 ± 1.023
3.603AlaThr: 3.603 ± 0.741
3.828AlaVal: 3.828 ± 1.291
0.225AlaTrp: 0.225 ± 0.149
1.239AlaTyr: 1.239 ± 0.358
0.0AlaXaa: 0.0 ± 0.0
Cys
0.225CysAla: 0.225 ± 0.169
0.113CysCys: 0.113 ± 0.138
0.901CysAsp: 0.901 ± 0.277
0.788CysGlu: 0.788 ± 0.338
0.225CysPhe: 0.225 ± 0.185
1.126CysGly: 1.126 ± 0.339
0.225CysHis: 0.225 ± 0.172
1.013CysIle: 1.013 ± 0.294
1.464CysLys: 1.464 ± 0.446
0.788CysLeu: 0.788 ± 0.406
0.225CysMet: 0.225 ± 0.18
0.901CysAsn: 0.901 ± 0.382
0.113CysPro: 0.113 ± 0.122
0.45CysGln: 0.45 ± 0.209
0.676CysArg: 0.676 ± 0.359
0.563CysSer: 0.563 ± 0.285
1.013CysThr: 1.013 ± 0.435
0.225CysVal: 0.225 ± 0.148
0.113CysTrp: 0.113 ± 0.114
0.563CysTyr: 0.563 ± 0.282
0.0CysXaa: 0.0 ± 0.0
Asp
1.802AspAla: 1.802 ± 0.608
0.563AspCys: 0.563 ± 0.238
2.702AspAsp: 2.702 ± 0.724
5.405AspGlu: 5.405 ± 0.804
3.04AspPhe: 3.04 ± 0.503
2.702AspGly: 2.702 ± 0.689
0.563AspHis: 0.563 ± 0.243
5.18AspIle: 5.18 ± 0.845
6.418AspLys: 6.418 ± 0.895
6.08AspLeu: 6.08 ± 0.825
1.802AspMet: 1.802 ± 0.409
2.928AspAsn: 2.928 ± 0.821
2.139AspPro: 2.139 ± 0.637
1.126AspGln: 1.126 ± 0.311
2.027AspArg: 2.027 ± 0.437
2.928AspSer: 2.928 ± 0.764
2.815AspThr: 2.815 ± 0.504
2.365AspVal: 2.365 ± 0.533
0.676AspTrp: 0.676 ± 0.246
2.139AspTyr: 2.139 ± 0.588
0.0AspXaa: 0.0 ± 0.0
Glu
4.391GluAla: 4.391 ± 0.771
1.239GluCys: 1.239 ± 0.505
4.504GluAsp: 4.504 ± 0.704
13.174GluGlu: 13.174 ± 1.891
5.18GluPhe: 5.18 ± 0.728
4.504GluGly: 4.504 ± 0.661
1.351GluHis: 1.351 ± 0.34
8.895GluIle: 8.895 ± 1.198
10.922GluLys: 10.922 ± 1.562
8.783GluLeu: 8.783 ± 1.452
2.59GluMet: 2.59 ± 0.61
6.531GluAsn: 6.531 ± 1.012
1.576GluPro: 1.576 ± 0.375
3.04GluGln: 3.04 ± 0.59
3.491GluArg: 3.491 ± 0.59
4.054GluSer: 4.054 ± 0.664
3.491GluThr: 3.491 ± 0.87
5.63GluVal: 5.63 ± 0.932
0.901GluTrp: 0.901 ± 0.291
4.166GluTyr: 4.166 ± 0.781
0.0GluXaa: 0.0 ± 0.0
Phe
1.013PheAla: 1.013 ± 0.302
0.676PheCys: 0.676 ± 0.267
2.252PheAsp: 2.252 ± 0.598
3.603PheGlu: 3.603 ± 0.506
2.252PhePhe: 2.252 ± 0.554
2.928PheGly: 2.928 ± 0.566
0.338PheHis: 0.338 ± 0.222
2.59PheIle: 2.59 ± 0.672
6.193PheLys: 6.193 ± 0.742
3.153PheLeu: 3.153 ± 0.716
1.013PheMet: 1.013 ± 0.334
2.702PheAsn: 2.702 ± 0.656
0.901PhePro: 0.901 ± 0.309
0.901PheGln: 0.901 ± 0.381
1.013PheArg: 1.013 ± 0.355
1.689PheSer: 1.689 ± 0.472
2.702PheThr: 2.702 ± 0.623
2.139PheVal: 2.139 ± 0.458
0.45PheTrp: 0.45 ± 0.178
2.139PheTyr: 2.139 ± 0.603
0.0PheXaa: 0.0 ± 0.0
Gly
3.491GlyAla: 3.491 ± 1.232
0.45GlyCys: 0.45 ± 0.229
3.04GlyAsp: 3.04 ± 0.485
4.842GlyGlu: 4.842 ± 0.762
2.59GlyPhe: 2.59 ± 0.51
3.153GlyGly: 3.153 ± 0.692
0.563GlyHis: 0.563 ± 0.343
4.166GlyIle: 4.166 ± 0.749
5.743GlyLys: 5.743 ± 0.637
4.617GlyLeu: 4.617 ± 1.025
2.139GlyMet: 2.139 ± 0.707
2.59GlyAsn: 2.59 ± 0.552
0.901GlyPro: 0.901 ± 0.28
1.464GlyGln: 1.464 ± 0.346
1.914GlyArg: 1.914 ± 0.383
3.04GlySer: 3.04 ± 0.529
2.59GlyThr: 2.59 ± 0.66
3.491GlyVal: 3.491 ± 0.771
1.013GlyTrp: 1.013 ± 0.3
1.802GlyTyr: 1.802 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
0.563HisAla: 0.563 ± 0.255
0.113HisCys: 0.113 ± 0.118
0.338HisAsp: 0.338 ± 0.181
1.126HisGlu: 1.126 ± 0.401
0.338HisPhe: 0.338 ± 0.205
0.788HisGly: 0.788 ± 0.261
0.563HisHis: 0.563 ± 0.374
0.788HisIle: 0.788 ± 0.28
1.126HisLys: 1.126 ± 0.397
1.126HisLeu: 1.126 ± 0.384
0.113HisMet: 0.113 ± 0.119
0.338HisAsn: 0.338 ± 0.194
0.225HisPro: 0.225 ± 0.119
0.338HisGln: 0.338 ± 0.203
0.45HisArg: 0.45 ± 0.21
0.563HisSer: 0.563 ± 0.308
0.113HisThr: 0.113 ± 0.092
0.563HisVal: 0.563 ± 0.198
0.0HisTrp: 0.0 ± 0.0
0.676HisTyr: 0.676 ± 0.285
0.0HisXaa: 0.0 ± 0.0
Ile
4.166IleAla: 4.166 ± 0.548
1.126IleCys: 1.126 ± 0.49
6.306IleAsp: 6.306 ± 0.898
7.657IleGlu: 7.657 ± 0.839
2.815IlePhe: 2.815 ± 0.614
3.04IleGly: 3.04 ± 0.815
0.563IleHis: 0.563 ± 0.256
6.756IleIle: 6.756 ± 0.872
9.458IleLys: 9.458 ± 1.135
6.08IleLeu: 6.08 ± 1.326
1.689IleMet: 1.689 ± 0.456
7.432IleAsn: 7.432 ± 1.194
1.689IlePro: 1.689 ± 0.523
2.139IleGln: 2.139 ± 0.379
3.265IleArg: 3.265 ± 0.537
4.617IleSer: 4.617 ± 0.707
3.603IleThr: 3.603 ± 0.68
3.265IleVal: 3.265 ± 0.679
0.676IleTrp: 0.676 ± 0.274
2.59IleTyr: 2.59 ± 0.462
0.0IleXaa: 0.0 ± 0.0
Lys
6.418LysAla: 6.418 ± 0.907
0.901LysCys: 0.901 ± 0.306
5.855LysAsp: 5.855 ± 0.829
14.188LysGlu: 14.188 ± 1.893
5.855LysPhe: 5.855 ± 0.788
4.842LysGly: 4.842 ± 0.657
0.676LysHis: 0.676 ± 0.221
8.895LysIle: 8.895 ± 1.029
11.936LysLys: 11.936 ± 1.427
10.81LysLeu: 10.81 ± 1.353
2.815LysMet: 2.815 ± 0.656
9.346LysAsn: 9.346 ± 1.298
1.126LysPro: 1.126 ± 0.37
4.166LysGln: 4.166 ± 0.719
5.855LysArg: 5.855 ± 0.845
6.643LysSer: 6.643 ± 0.8
5.405LysThr: 5.405 ± 0.604
6.756LysVal: 6.756 ± 0.809
1.351LysTrp: 1.351 ± 0.487
4.504LysTyr: 4.504 ± 1.016
0.0LysXaa: 0.0 ± 0.0
Leu
5.517LeuAla: 5.517 ± 1.037
0.788LeuCys: 0.788 ± 0.396
4.166LeuAsp: 4.166 ± 0.699
10.697LeuGlu: 10.697 ± 1.098
2.477LeuPhe: 2.477 ± 0.496
5.292LeuGly: 5.292 ± 0.885
0.338LeuHis: 0.338 ± 0.166
5.292LeuIle: 5.292 ± 0.927
11.598LeuLys: 11.598 ± 1.112
7.544LeuLeu: 7.544 ± 1.219
1.914LeuMet: 1.914 ± 0.481
8.107LeuAsn: 8.107 ± 0.77
1.914LeuPro: 1.914 ± 0.427
2.252LeuGln: 2.252 ± 0.538
3.941LeuArg: 3.941 ± 0.784
4.842LeuSer: 4.842 ± 0.634
5.18LeuThr: 5.18 ± 0.99
4.279LeuVal: 4.279 ± 0.741
0.45LeuTrp: 0.45 ± 0.218
2.252LeuTyr: 2.252 ± 0.488
0.0LeuXaa: 0.0 ± 0.0
Met
2.252MetAla: 2.252 ± 0.569
0.45MetCys: 0.45 ± 0.222
1.239MetAsp: 1.239 ± 0.398
1.689MetGlu: 1.689 ± 0.393
0.676MetPhe: 0.676 ± 0.26
1.126MetGly: 1.126 ± 0.276
0.225MetHis: 0.225 ± 0.16
1.576MetIle: 1.576 ± 0.37
3.491MetLys: 3.491 ± 0.623
2.928MetLeu: 2.928 ± 0.579
0.45MetMet: 0.45 ± 0.188
1.126MetAsn: 1.126 ± 0.405
0.113MetPro: 0.113 ± 0.123
0.563MetGln: 0.563 ± 0.249
0.901MetArg: 0.901 ± 0.265
3.153MetSer: 3.153 ± 0.573
0.901MetThr: 0.901 ± 0.284
1.464MetVal: 1.464 ± 0.323
0.0MetTrp: 0.0 ± 0.0
0.563MetTyr: 0.563 ± 0.243
0.0MetXaa: 0.0 ± 0.0
Asn
4.391AsnAla: 4.391 ± 0.802
0.338AsnCys: 0.338 ± 0.217
3.716AsnAsp: 3.716 ± 0.458
5.18AsnGlu: 5.18 ± 0.824
4.391AsnPhe: 4.391 ± 0.734
4.391AsnGly: 4.391 ± 0.746
1.013AsnHis: 1.013 ± 0.365
6.756AsnIle: 6.756 ± 1.069
7.432AsnLys: 7.432 ± 1.199
5.517AsnLeu: 5.517 ± 0.83
1.914AsnMet: 1.914 ± 0.473
4.617AsnAsn: 4.617 ± 0.845
0.676AsnPro: 0.676 ± 0.334
2.252AsnGln: 2.252 ± 0.527
2.027AsnArg: 2.027 ± 0.633
4.842AsnSer: 4.842 ± 0.842
2.815AsnThr: 2.815 ± 0.481
3.941AsnVal: 3.941 ± 0.723
1.464AsnTrp: 1.464 ± 0.459
1.802AsnTyr: 1.802 ± 0.613
0.0AsnXaa: 0.0 ± 0.0
Pro
1.239ProAla: 1.239 ± 0.327
0.225ProCys: 0.225 ± 0.164
0.901ProAsp: 0.901 ± 0.261
1.802ProGlu: 1.802 ± 0.463
0.563ProPhe: 0.563 ± 0.285
0.45ProGly: 0.45 ± 0.236
0.45ProHis: 0.45 ± 0.261
1.576ProIle: 1.576 ± 0.504
1.351ProLys: 1.351 ± 0.392
1.351ProLeu: 1.351 ± 0.456
0.225ProMet: 0.225 ± 0.167
1.239ProAsn: 1.239 ± 0.367
0.225ProPro: 0.225 ± 0.185
0.45ProGln: 0.45 ± 0.207
0.676ProArg: 0.676 ± 0.244
1.013ProSer: 1.013 ± 0.287
1.576ProThr: 1.576 ± 0.467
1.689ProVal: 1.689 ± 0.528
0.0ProTrp: 0.0 ± 0.0
1.013ProTyr: 1.013 ± 0.372
0.0ProXaa: 0.0 ± 0.0
Gln
2.59GlnAla: 2.59 ± 0.558
0.0GlnCys: 0.0 ± 0.0
1.013GlnAsp: 1.013 ± 0.336
2.252GlnGlu: 2.252 ± 0.609
0.563GlnPhe: 0.563 ± 0.268
1.351GlnGly: 1.351 ± 0.337
0.225GlnHis: 0.225 ± 0.184
1.689GlnIle: 1.689 ± 0.447
3.491GlnLys: 3.491 ± 0.624
3.265GlnLeu: 3.265 ± 0.553
0.901GlnMet: 0.901 ± 0.32
1.914GlnAsn: 1.914 ± 0.455
0.45GlnPro: 0.45 ± 0.241
1.126GlnGln: 1.126 ± 0.453
1.576GlnArg: 1.576 ± 0.332
1.914GlnSer: 1.914 ± 0.367
0.788GlnThr: 0.788 ± 0.372
1.802GlnVal: 1.802 ± 0.454
0.113GlnTrp: 0.113 ± 0.12
1.351GlnTyr: 1.351 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
2.252ArgAla: 2.252 ± 0.52
0.901ArgCys: 0.901 ± 0.44
2.139ArgAsp: 2.139 ± 0.418
4.166ArgGlu: 4.166 ± 0.779
1.239ArgPhe: 1.239 ± 0.512
0.676ArgGly: 0.676 ± 0.264
0.338ArgHis: 0.338 ± 0.167
4.617ArgIle: 4.617 ± 0.612
4.842ArgLys: 4.842 ± 0.907
4.279ArgLeu: 4.279 ± 0.791
2.027ArgMet: 2.027 ± 0.44
2.139ArgAsn: 2.139 ± 0.349
0.45ArgPro: 0.45 ± 0.216
1.239ArgGln: 1.239 ± 0.324
1.689ArgArg: 1.689 ± 0.502
1.239ArgSer: 1.239 ± 0.342
0.901ArgThr: 0.901 ± 0.282
2.252ArgVal: 2.252 ± 0.492
0.113ArgTrp: 0.113 ± 0.108
1.914ArgTyr: 1.914 ± 0.603
0.0ArgXaa: 0.0 ± 0.0
Ser
2.027SerAla: 2.027 ± 0.453
1.239SerCys: 1.239 ± 0.414
3.603SerAsp: 3.603 ± 0.731
4.842SerGlu: 4.842 ± 0.803
2.139SerPhe: 2.139 ± 0.424
2.815SerGly: 2.815 ± 0.714
0.676SerHis: 0.676 ± 0.256
4.954SerIle: 4.954 ± 0.67
6.531SerLys: 6.531 ± 0.783
5.855SerLeu: 5.855 ± 0.952
0.901SerMet: 0.901 ± 0.278
4.504SerAsn: 4.504 ± 0.946
1.126SerPro: 1.126 ± 0.303
1.576SerGln: 1.576 ± 0.444
2.477SerArg: 2.477 ± 0.594
3.828SerSer: 3.828 ± 0.891
2.477SerThr: 2.477 ± 0.777
3.603SerVal: 3.603 ± 0.583
0.788SerTrp: 0.788 ± 0.29
1.914SerTyr: 1.914 ± 0.513
0.0SerXaa: 0.0 ± 0.0
Thr
3.491ThrAla: 3.491 ± 1.258
0.338ThrCys: 0.338 ± 0.18
2.365ThrAsp: 2.365 ± 0.521
4.166ThrGlu: 4.166 ± 0.721
1.576ThrPhe: 1.576 ± 0.419
3.941ThrGly: 3.941 ± 0.998
0.338ThrHis: 0.338 ± 0.159
3.378ThrIle: 3.378 ± 0.657
4.617ThrLys: 4.617 ± 0.735
4.166ThrLeu: 4.166 ± 0.794
0.788ThrMet: 0.788 ± 0.3
2.815ThrAsn: 2.815 ± 0.56
1.013ThrPro: 1.013 ± 0.265
1.576ThrGln: 1.576 ± 0.556
2.027ThrArg: 2.027 ± 0.582
3.378ThrSer: 3.378 ± 0.738
2.365ThrThr: 2.365 ± 0.804
3.265ThrVal: 3.265 ± 0.717
0.338ThrTrp: 0.338 ± 0.239
1.802ThrTyr: 1.802 ± 0.49
0.0ThrXaa: 0.0 ± 0.0
Val
3.378ValAla: 3.378 ± 0.947
0.901ValCys: 0.901 ± 0.231
4.391ValAsp: 4.391 ± 0.623
5.743ValGlu: 5.743 ± 1.044
1.576ValPhe: 1.576 ± 0.46
3.603ValGly: 3.603 ± 0.974
0.338ValHis: 0.338 ± 0.259
3.04ValIle: 3.04 ± 0.767
7.995ValLys: 7.995 ± 0.908
4.166ValLeu: 4.166 ± 0.703
0.788ValMet: 0.788 ± 0.264
4.279ValAsn: 4.279 ± 0.781
1.689ValPro: 1.689 ± 0.526
1.689ValGln: 1.689 ± 0.4
1.689ValArg: 1.689 ± 0.478
2.815ValSer: 2.815 ± 0.548
3.491ValThr: 3.491 ± 0.626
2.815ValVal: 2.815 ± 0.563
0.676ValTrp: 0.676 ± 0.315
1.464ValTyr: 1.464 ± 0.334
0.0ValXaa: 0.0 ± 0.0
Trp
0.338TrpAla: 0.338 ± 0.19
0.113TrpCys: 0.113 ± 0.127
0.563TrpAsp: 0.563 ± 0.26
0.676TrpGlu: 0.676 ± 0.232
0.225TrpPhe: 0.225 ± 0.166
0.338TrpGly: 0.338 ± 0.218
0.225TrpHis: 0.225 ± 0.14
1.239TrpIle: 1.239 ± 0.445
1.239TrpLys: 1.239 ± 0.306
0.901TrpLeu: 0.901 ± 0.354
0.0TrpMet: 0.0 ± 0.0
0.563TrpAsn: 0.563 ± 0.241
0.0TrpPro: 0.0 ± 0.0
0.113TrpGln: 0.113 ± 0.128
0.338TrpArg: 0.338 ± 0.209
0.788TrpSer: 0.788 ± 0.321
0.225TrpThr: 0.225 ± 0.136
1.013TrpVal: 1.013 ± 0.425
0.113TrpTrp: 0.113 ± 0.092
0.676TrpTyr: 0.676 ± 0.271
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.914TyrAla: 1.914 ± 0.307
0.901TyrCys: 0.901 ± 0.319
2.027TyrAsp: 2.027 ± 0.489
2.027TyrGlu: 2.027 ± 0.434
1.464TyrPhe: 1.464 ± 0.347
1.802TyrGly: 1.802 ± 0.511
0.45TyrHis: 0.45 ± 0.185
1.914TyrIle: 1.914 ± 0.476
5.067TyrLys: 5.067 ± 1.046
4.166TyrLeu: 4.166 ± 0.781
0.901TyrMet: 0.901 ± 0.384
2.702TyrAsn: 2.702 ± 0.506
0.676TyrPro: 0.676 ± 0.23
0.338TyrGln: 0.338 ± 0.164
1.689TyrArg: 1.689 ± 0.505
2.477TyrSer: 2.477 ± 0.549
1.689TyrThr: 1.689 ± 0.371
2.139TyrVal: 2.139 ± 0.521
0.225TyrTrp: 0.225 ± 0.167
1.239TyrTyr: 1.239 ± 0.448
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (8882 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski