Bacillus sp. CHD6a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3740 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9GV97|A0A0M9GV97_9BACI 5'-3' exonuclease OS=Bacillus sp. CHD6a OX=1643452 GN=AAV98_02130 PE=4 SV=1
MM1 pKa = 6.2YY2 pKa = 9.31TIYY5 pKa = 11.13NKK7 pKa = 11.12GEE9 pKa = 4.18LDD11 pKa = 3.65GLASPTYY18 pKa = 8.22WWPEE22 pKa = 3.65DD23 pKa = 3.98RR24 pKa = 11.84SWCVSTDD31 pKa = 2.98YY32 pKa = 11.58DD33 pKa = 4.85LDD35 pKa = 3.72FTIFGGNKK43 pKa = 8.88QLFDD47 pKa = 3.91ALMFNDD53 pKa = 3.79KK54 pKa = 10.83LEE56 pKa = 4.76CIEE59 pKa = 4.26VDD61 pKa = 3.28LEE63 pKa = 4.28TRR65 pKa = 11.84IDD67 pKa = 3.62EE68 pKa = 4.35

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M9GT15|A0A0M9GT15_9BACI Uncharacterized protein OS=Bacillus sp. CHD6a OX=1643452 GN=AAV98_08550 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84SKK14 pKa = 9.25VHH16 pKa = 5.86GFRR19 pKa = 11.84EE20 pKa = 4.33RR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3740

0

3740

1060701

26

1921

283.6

31.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.755 ± 0.048

0.697 ± 0.011

5.03 ± 0.031

7.909 ± 0.052

4.613 ± 0.037

6.836 ± 0.042

2.129 ± 0.021

7.519 ± 0.041

6.914 ± 0.041

9.865 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.888 ± 0.017

4.285 ± 0.027

3.572 ± 0.025

3.651 ± 0.025

4.002 ± 0.029

5.984 ± 0.031

5.474 ± 0.023

7.31 ± 0.035

1.036 ± 0.016

3.532 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski