Amino acid dipepetide frequency for Bacillus sp. CHD6a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.264AlaAla: 5.264 ± 0.101	0.507AlaCys: 0.507 ± 0.021	3.147AlaAsp: 3.147 ± 0.055	4.575AlaGlu: 4.575 ± 0.076	3.236AlaPhe: 3.236 ± 0.056	5.093AlaGly: 5.093 ± 0.087	1.271AlaHis: 1.271 ± 0.04	5.559AlaIle: 5.559 ± 0.076	4.569AlaLys: 4.569 ± 0.077	6.953AlaLeu: 6.953 ± 0.095	2.073AlaMet: 2.073 ± 0.049	2.653AlaAsn: 2.653 ± 0.049	2.118AlaPro: 2.118 ± 0.047	2.102AlaGln: 2.102 ± 0.045	2.575AlaArg: 2.575 ± 0.055	4.118AlaSer: 4.118 ± 0.066	3.635AlaThr: 3.635 ± 0.065	5.24AlaVal: 5.24 ± 0.076	0.616AlaTrp: 0.616 ± 0.025	2.247AlaTyr: 2.247 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.419CysAla: 0.419 ± 0.022	0.102CysCys: 0.102 ± 0.012	0.387CysAsp: 0.387 ± 0.02	0.475CysGlu: 0.475 ± 0.023	0.299CysPhe: 0.299 ± 0.017	0.6CysGly: 0.6 ± 0.027	0.203CysHis: 0.203 ± 0.015	0.505CysIle: 0.505 ± 0.022	0.378CysLys: 0.378 ± 0.021	0.648CysLeu: 0.648 ± 0.026	0.186CysMet: 0.186 ± 0.013	0.305CysAsn: 0.305 ± 0.016	0.337CysPro: 0.337 ± 0.02	0.217CysGln: 0.217 ± 0.016	0.267CysArg: 0.267 ± 0.016	0.491CysSer: 0.491 ± 0.025	0.397CysThr: 0.397 ± 0.021	0.424CysVal: 0.424 ± 0.026	0.07CysTrp: 0.07 ± 0.009	0.259CysTyr: 0.259 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.268AspAla: 3.268 ± 0.064	0.38AspCys: 0.38 ± 0.021	2.252AspAsp: 2.252 ± 0.054	4.263AspGlu: 4.263 ± 0.07	2.462AspPhe: 2.462 ± 0.047	3.345AspGly: 3.345 ± 0.065	1.091AspHis: 1.091 ± 0.035	4.045AspIle: 4.045 ± 0.06	3.179AspLys: 3.179 ± 0.056	4.92AspLeu: 4.92 ± 0.064	1.456AspMet: 1.456 ± 0.042	1.822AspAsn: 1.822 ± 0.046	1.948AspPro: 1.948 ± 0.044	1.852AspGln: 1.852 ± 0.04	2.262AspArg: 2.262 ± 0.048	2.628AspSer: 2.628 ± 0.054	2.46AspThr: 2.46 ± 0.054	3.908AspVal: 3.908 ± 0.062	0.62AspTrp: 0.62 ± 0.028	2.137AspTyr: 2.137 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.503GluAla: 5.503 ± 0.079	0.395GluCys: 0.395 ± 0.022	4.176GluAsp: 4.176 ± 0.062	8.314GluGlu: 8.314 ± 0.112	2.714GluPhe: 2.714 ± 0.05	4.705GluGly: 4.705 ± 0.078	1.609GluHis: 1.609 ± 0.042	5.49GluIle: 5.49 ± 0.081	7.002GluLys: 7.002 ± 0.103	7.527GluLeu: 7.527 ± 0.1	2.409GluMet: 2.409 ± 0.05	3.728GluAsn: 3.728 ± 0.067	1.996GluPro: 1.996 ± 0.048	3.285GluGln: 3.285 ± 0.06	3.535GluArg: 3.535 ± 0.067	3.608GluSer: 3.608 ± 0.059	4.008GluThr: 4.008 ± 0.061	5.755GluVal: 5.755 ± 0.072	0.921GluTrp: 0.921 ± 0.035	2.412GluTyr: 2.412 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	2.977PheAla: 2.977 ± 0.057	0.35PheCys: 0.35 ± 0.018	2.271PheAsp: 2.271 ± 0.046	2.962PheGlu: 2.962 ± 0.054	2.518PhePhe: 2.518 ± 0.064	3.35PheGly: 3.35 ± 0.06	1.138PheHis: 1.138 ± 0.032	3.844PheIle: 3.844 ± 0.075	2.264PheLys: 2.264 ± 0.043	4.771PheLeu: 4.771 ± 0.077	1.244PheMet: 1.244 ± 0.034	1.809PheAsn: 1.809 ± 0.044	1.692PhePro: 1.692 ± 0.039	1.725PheGln: 1.725 ± 0.044	1.596PheArg: 1.596 ± 0.04	3.349PheSer: 3.349 ± 0.052	2.704PheThr: 2.704 ± 0.051	3.268PheVal: 3.268 ± 0.063	0.483PheTrp: 0.483 ± 0.023	1.814PheTyr: 1.814 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.763GlyAla: 4.763 ± 0.079	0.585GlyCys: 0.585 ± 0.024	3.22GlyAsp: 3.22 ± 0.055	4.849GlyGlu: 4.849 ± 0.072	3.387GlyPhe: 3.387 ± 0.057	4.825GlyGly: 4.825 ± 0.081	1.32GlyHis: 1.32 ± 0.041	5.535GlyIle: 5.535 ± 0.075	4.972GlyLys: 4.972 ± 0.074	6.546GlyLeu: 6.546 ± 0.097	2.262GlyMet: 2.262 ± 0.046	2.733GlyAsn: 2.733 ± 0.053	1.71GlyPro: 1.71 ± 0.046	2.055GlyGln: 2.055 ± 0.05	2.704GlyArg: 2.704 ± 0.049	4.025GlySer: 4.025 ± 0.069	3.991GlyThr: 3.991 ± 0.072	5.364GlyVal: 5.364 ± 0.085	0.819GlyTrp: 0.819 ± 0.034	2.692GlyTyr: 2.692 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.355HisAla: 1.355 ± 0.037	0.178HisCys: 0.178 ± 0.014	1.036HisAsp: 1.036 ± 0.033	1.431HisGlu: 1.431 ± 0.047	1.086HisPhe: 1.086 ± 0.031	1.363HisGly: 1.363 ± 0.037	0.688HisHis: 0.688 ± 0.028	1.538HisIle: 1.538 ± 0.041	1.127HisLys: 1.127 ± 0.038	2.165HisLeu: 2.165 ± 0.054	0.502HisMet: 0.502 ± 0.019	0.822HisAsn: 0.822 ± 0.027	1.106HisPro: 1.106 ± 0.033	0.865HisGln: 0.865 ± 0.027	0.911HisArg: 0.911 ± 0.031	1.291HisSer: 1.291 ± 0.04	1.148HisThr: 1.148 ± 0.03	1.532HisVal: 1.532 ± 0.039	0.215HisTrp: 0.215 ± 0.013	0.937HisTyr: 0.937 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.615IleAla: 5.615 ± 0.081	0.605IleCys: 0.605 ± 0.025	3.92IleAsp: 3.92 ± 0.067	5.149IleGlu: 5.149 ± 0.084	3.37IlePhe: 3.37 ± 0.065	5.738IleGly: 5.738 ± 0.08	1.686IleHis: 1.686 ± 0.042	5.695IleIle: 5.695 ± 0.09	4.493IleLys: 4.493 ± 0.066	7.046IleLeu: 7.046 ± 0.09	1.834IleMet: 1.834 ± 0.043	3.248IleAsn: 3.248 ± 0.072	3.304IlePro: 3.304 ± 0.051	2.79IleGln: 2.79 ± 0.046	2.949IleArg: 2.949 ± 0.063	4.926IleSer: 4.926 ± 0.08	4.33IleThr: 4.33 ± 0.073	5.403IleVal: 5.403 ± 0.076	0.637IleTrp: 0.637 ± 0.025	2.452IleTyr: 2.452 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.505LysAla: 4.505 ± 0.069	0.333LysCys: 0.333 ± 0.02	3.903LysAsp: 3.903 ± 0.056	7.466LysGlu: 7.466 ± 0.096	1.943LysPhe: 1.943 ± 0.043	4.546LysGly: 4.546 ± 0.07	1.385LysHis: 1.385 ± 0.035	4.35LysIle: 4.35 ± 0.068	5.857LysLys: 5.857 ± 0.095	6.04LysLeu: 6.04 ± 0.073	2.174LysMet: 2.174 ± 0.048	3.238LysAsn: 3.238 ± 0.055	2.146LysPro: 2.146 ± 0.048	2.951LysGln: 2.951 ± 0.057	3.351LysArg: 3.351 ± 0.061	3.438LysSer: 3.438 ± 0.064	3.407LysThr: 3.407 ± 0.058	5.108LysVal: 5.108 ± 0.073	0.824LysTrp: 0.824 ± 0.034	2.179LysTyr: 2.179 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	7.076LeuAla: 7.076 ± 0.093	0.69LeuCys: 0.69 ± 0.027	4.817LeuAsp: 4.817 ± 0.071	7.445LeuGlu: 7.445 ± 0.102	5.026LeuPhe: 5.026 ± 0.092	6.513LeuGly: 6.513 ± 0.085	2.118LeuHis: 2.118 ± 0.046	6.911LeuIle: 6.911 ± 0.095	6.457LeuLys: 6.457 ± 0.074	10.536LeuLeu: 10.536 ± 0.135	2.562LeuMet: 2.562 ± 0.06	4.0LeuAsn: 4.0 ± 0.07	3.947LeuPro: 3.947 ± 0.07	3.831LeuGln: 3.831 ± 0.07	3.61LeuArg: 3.61 ± 0.067	6.614LeuSer: 6.614 ± 0.082	5.725LeuThr: 5.725 ± 0.069	6.78LeuVal: 6.78 ± 0.09	0.822LeuTrp: 0.822 ± 0.029	3.165LeuTyr: 3.165 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	1.991MetAla: 1.991 ± 0.045	0.136MetCys: 0.136 ± 0.012	1.651MetAsp: 1.651 ± 0.04	2.614MetGlu: 2.614 ± 0.054	1.152MetPhe: 1.152 ± 0.034	1.811MetGly: 1.811 ± 0.046	0.463MetHis: 0.463 ± 0.021	1.981MetIle: 1.981 ± 0.05	2.615MetLys: 2.615 ± 0.046	2.736MetLeu: 2.736 ± 0.058	0.917MetMet: 0.917 ± 0.033	1.545MetAsn: 1.545 ± 0.035	0.951MetPro: 0.951 ± 0.031	0.973MetGln: 0.973 ± 0.029	1.092MetArg: 1.092 ± 0.032	1.591MetSer: 1.591 ± 0.034	1.659MetThr: 1.659 ± 0.042	2.007MetVal: 2.007 ± 0.04	0.224MetTrp: 0.224 ± 0.016	0.773MetTyr: 0.773 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.527AsnAla: 2.527 ± 0.051	0.283AsnCys: 0.283 ± 0.016	2.05AsnAsp: 2.05 ± 0.049	3.385AsnGlu: 3.385 ± 0.066	1.681AsnPhe: 1.681 ± 0.039	3.183AsnGly: 3.183 ± 0.059	1.012AsnHis: 1.012 ± 0.034	3.272AsnIle: 3.272 ± 0.063	2.998AsnLys: 2.998 ± 0.054	3.828AsnLeu: 3.828 ± 0.066	1.267AsnMet: 1.267 ± 0.032	2.013AsnAsn: 2.013 ± 0.049	1.977AsnPro: 1.977 ± 0.04	1.782AsnGln: 1.782 ± 0.045	1.995AsnArg: 1.995 ± 0.041	2.306AsnSer: 2.306 ± 0.049	2.106AsnThr: 2.106 ± 0.058	3.106AsnVal: 3.106 ± 0.064	0.506AsnTrp: 0.506 ± 0.023	1.573AsnTyr: 1.573 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.18ProAla: 2.18 ± 0.051	0.212ProCys: 0.212 ± 0.017	1.906ProAsp: 1.906 ± 0.047	2.868ProGlu: 2.868 ± 0.062	1.982ProPhe: 1.982 ± 0.049	2.266ProGly: 2.266 ± 0.051	0.766ProHis: 0.766 ± 0.029	2.769ProIle: 2.769 ± 0.053	2.21ProLys: 2.21 ± 0.047	3.447ProLeu: 3.447 ± 0.062	0.875ProMet: 0.875 ± 0.028	1.644ProAsn: 1.644 ± 0.036	1.103ProPro: 1.103 ± 0.037	1.112ProGln: 1.112 ± 0.032	1.127ProArg: 1.127 ± 0.034	2.464ProSer: 2.464 ± 0.05	2.138ProThr: 2.138 ± 0.05	2.85ProVal: 2.85 ± 0.048	0.343ProTrp: 0.343 ± 0.017	1.458ProTyr: 1.458 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	2.693GlnAla: 2.693 ± 0.05	0.188GlnCys: 0.188 ± 0.012	1.769GlnAsp: 1.769 ± 0.038	3.131GlnGlu: 3.131 ± 0.051	1.462GlnPhe: 1.462 ± 0.036	2.167GlnGly: 2.167 ± 0.046	0.819GlnHis: 0.819 ± 0.031	2.321GlnIle: 2.321 ± 0.044	2.65GlnLys: 2.65 ± 0.052	3.887GlnLeu: 3.887 ± 0.074	1.079GlnMet: 1.079 ± 0.028	1.524GlnAsn: 1.524 ± 0.042	1.221GlnPro: 1.221 ± 0.035	1.836GlnGln: 1.836 ± 0.051	1.486GlnArg: 1.486 ± 0.035	2.089GlnSer: 2.089 ± 0.043	2.03GlnThr: 2.03 ± 0.041	2.456GlnVal: 2.456 ± 0.055	0.44GlnTrp: 0.44 ± 0.02	1.261GlnTyr: 1.261 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.397ArgAla: 2.397 ± 0.056	0.259ArgCys: 0.259 ± 0.016	2.072ArgAsp: 2.072 ± 0.049	3.319ArgGlu: 3.319 ± 0.064	1.828ArgPhe: 1.828 ± 0.039	2.432ArgGly: 2.432 ± 0.047	0.817ArgHis: 0.817 ± 0.027	2.904ArgIle: 2.904 ± 0.06	3.324ArgLys: 3.324 ± 0.067	3.953ArgLeu: 3.953 ± 0.069	1.368ArgMet: 1.368 ± 0.036	1.87ArgAsn: 1.87 ± 0.038	1.296ArgPro: 1.296 ± 0.035	1.389ArgGln: 1.389 ± 0.034	1.775ArgArg: 1.775 ± 0.046	2.255ArgSer: 2.255 ± 0.047	2.082ArgThr: 2.082 ± 0.039	2.743ArgVal: 2.743 ± 0.053	0.42ArgTrp: 0.42 ± 0.02	1.515ArgTyr: 1.515 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.497SerAla: 3.497 ± 0.058	0.406SerCys: 0.406 ± 0.022	2.71SerAsp: 2.71 ± 0.06	4.044SerGlu: 4.044 ± 0.063	3.534SerPhe: 3.534 ± 0.066	4.323SerGly: 4.323 ± 0.066	1.224SerHis: 1.224 ± 0.041	5.161SerIle: 5.161 ± 0.07	3.835SerLys: 3.835 ± 0.063	6.244SerLeu: 6.244 ± 0.072	1.791SerMet: 1.791 ± 0.041	2.561SerAsn: 2.561 ± 0.047	2.091SerPro: 2.091 ± 0.048	1.879SerGln: 1.879 ± 0.04	2.166SerArg: 2.166 ± 0.045	3.939SerSer: 3.939 ± 0.064	3.297SerThr: 3.297 ± 0.064	4.234SerVal: 4.234 ± 0.063	0.63SerTrp: 0.63 ± 0.025	2.278SerTyr: 2.278 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	3.668ThrAla: 3.668 ± 0.07	0.386ThrCys: 0.386 ± 0.019	2.675ThrAsp: 2.675 ± 0.053	3.716ThrGlu: 3.716 ± 0.067	2.753ThrPhe: 2.753 ± 0.058	4.034ThrGly: 4.034 ± 0.064	1.116ThrHis: 1.116 ± 0.038	4.584ThrIle: 4.584 ± 0.071	3.486ThrLys: 3.486 ± 0.058	5.592ThrLeu: 5.592 ± 0.072	1.417ThrMet: 1.417 ± 0.034	2.487ThrAsn: 2.487 ± 0.053	2.307ThrPro: 2.307 ± 0.045	1.54ThrGln: 1.54 ± 0.032	1.866ThrArg: 1.866 ± 0.043	3.34ThrSer: 3.34 ± 0.056	2.941ThrThr: 2.941 ± 0.063	4.289ThrVal: 4.289 ± 0.075	0.535ThrTrp: 0.535 ± 0.025	2.01ThrTyr: 2.01 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	5.198ValAla: 5.198 ± 0.079	0.614ValCys: 0.614 ± 0.026	3.887ValAsp: 3.887 ± 0.073	5.62ValGlu: 5.62 ± 0.083	3.225ValPhe: 3.225 ± 0.056	5.038ValGly: 5.038 ± 0.092	1.478ValHis: 1.478 ± 0.041	5.514ValIle: 5.514 ± 0.075	4.849ValLys: 4.849 ± 0.07	7.085ValLeu: 7.085 ± 0.092	2.147ValMet: 2.147 ± 0.048	2.904ValAsn: 2.904 ± 0.057	2.774ValPro: 2.774 ± 0.056	2.463ValGln: 2.463 ± 0.048	2.733ValArg: 2.733 ± 0.058	4.609ValSer: 4.609 ± 0.072	4.357ValThr: 4.357 ± 0.073	5.626ValVal: 5.626 ± 0.093	0.683ValTrp: 0.683 ± 0.026	2.292ValTyr: 2.292 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.584TrpAla: 0.584 ± 0.025	0.071TrpCys: 0.071 ± 0.009	0.558TrpAsp: 0.558 ± 0.021	0.758TrpGlu: 0.758 ± 0.03	0.513TrpPhe: 0.513 ± 0.02	0.669TrpGly: 0.669 ± 0.028	0.201TrpHis: 0.201 ± 0.013	0.804TrpIle: 0.804 ± 0.033	0.846TrpLys: 0.846 ± 0.032	1.08TrpLeu: 1.08 ± 0.034	0.397TrpMet: 0.397 ± 0.02	0.552TrpAsn: 0.552 ± 0.022	0.251TrpPro: 0.251 ± 0.016	0.35TrpGln: 0.35 ± 0.02	0.396TrpArg: 0.396 ± 0.02	0.606TrpSer: 0.606 ± 0.029	0.527TrpThr: 0.527 ± 0.022	0.663TrpVal: 0.663 ± 0.026	0.172TrpTrp: 0.172 ± 0.011	0.361TrpTyr: 0.361 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.073TyrAla: 2.073 ± 0.037	0.288TyrCys: 0.288 ± 0.018	1.891TyrAsp: 1.891 ± 0.051	2.709TyrGlu: 2.709 ± 0.063	1.956TyrPhe: 1.956 ± 0.042	2.354TyrGly: 2.354 ± 0.043	0.889TyrHis: 0.889 ± 0.025	2.41TyrIle: 2.41 ± 0.05	2.033TyrLys: 2.033 ± 0.043	3.642TyrLeu: 3.642 ± 0.067	0.925TyrMet: 0.925 ± 0.029	1.391TyrAsn: 1.391 ± 0.04	1.445TyrPro: 1.445 ± 0.038	1.514TyrGln: 1.514 ± 0.041	1.624TyrArg: 1.624 ± 0.041	2.155TyrSer: 2.155 ± 0.047	1.8TyrThr: 1.8 ± 0.042	2.34TyrVal: 2.34 ± 0.047	0.377TyrTrp: 0.377 ± 0.018	1.5TyrTyr: 1.5 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3740 proteins (1060701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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