Jannaschia rubra
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3629 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M6XKY5|A0A0M6XKY5_9RHOB Heparinase II/III-like protein OS=Jannaschia rubra OX=282197 GN=JAN5088_00346 PE=4 SV=1
MM1 pKa = 7.52 LRR3 pKa = 11.84 TLSTAALAVTLAVPAFAQDD22 pKa = 3.42 MADD25 pKa = 3.22 MSDD28 pKa = 4.47 LIRR31 pKa = 11.84 SRR33 pKa = 11.84 DD34 pKa = 3.39 ITGGPVYY41 pKa = 10.64 SIANNYY47 pKa = 9.52 DD48 pKa = 3.15 EE49 pKa = 5.05 NTWLDD54 pKa = 3.67 LNDD57 pKa = 3.99 RR58 pKa = 11.84 DD59 pKa = 4.14 SYY61 pKa = 11.78 GYY63 pKa = 10.99 NNYY66 pKa = 10.56 GYY68 pKa = 8.78 GTDD71 pKa = 3.59 YY72 pKa = 11.28 NQIGEE77 pKa = 4.3 IEE79 pKa = 4.65 DD80 pKa = 3.9 VILDD84 pKa = 3.71 KK85 pKa = 11.06 GGQMVGIVAEE95 pKa = 4.18 VGGFLDD101 pKa = 4.33 IGDD104 pKa = 4.01 KK105 pKa = 10.84 HH106 pKa = 7.75 VMVPVDD112 pKa = 4.95 DD113 pKa = 4.1 IRR115 pKa = 11.84 LVAVDD120 pKa = 4.19 DD121 pKa = 4.29 ASYY124 pKa = 11.19 SYY126 pKa = 9.3 VTRR129 pKa = 11.84 LSEE132 pKa = 4.14 EE133 pKa = 3.93 QLEE136 pKa = 4.25 EE137 pKa = 4.23 LPSVDD142 pKa = 4.47 EE143 pKa = 4.62 GWWDD147 pKa = 3.22
Molecular weight: 16.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.478
ProMoST 3.834
Dawson 3.706
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.528
Grimsley 3.389
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.088
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A0M6XTL3|A0A0M6XTL3_9RHOB GIY-YIG domain-containing protein OS=Jannaschia rubra OX=282197 GN=JAN5088_03216 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.83 HH14 pKa = 4.64 RR15 pKa = 11.84 HH16 pKa = 3.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.97 VLSAA44 pKa = 4.11
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3629
0
3629
1106816
29
2957
305.0
32.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.164 ± 0.053
0.843 ± 0.013
6.681 ± 0.04
5.525 ± 0.039
3.499 ± 0.024
9.14 ± 0.041
2.03 ± 0.017
4.704 ± 0.03
2.26 ± 0.027
10.055 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.748 ± 0.02
2.139 ± 0.019
5.422 ± 0.031
2.737 ± 0.022
7.847 ± 0.041
4.602 ± 0.026
5.606 ± 0.029
7.601 ± 0.037
1.457 ± 0.02
1.941 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here