Sulfurisoma sediminicola
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2823 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A497X9K6|A0A497X9K6_9PROT Nitric oxide reductase NorE protein OS=Sulfurisoma sediminicola OX=1381557 GN=DFR35_2501 PE=3 SV=1
MM1 pKa = 7.41 NAPNEE6 pKa = 4.16 MPAPFNFTDD15 pKa = 3.42 SAANKK20 pKa = 9.49 VKK22 pKa = 10.47 EE23 pKa = 4.3 LIQEE27 pKa = 4.22 EE28 pKa = 4.85 GNPDD32 pKa = 2.91 LKK34 pKa = 11.14 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.9 GFTFDD54 pKa = 3.69 EE55 pKa = 4.74 VANEE59 pKa = 4.76 DD60 pKa = 3.73 DD61 pKa = 3.24 TSMVKK66 pKa = 10.61 NGVTLLIDD74 pKa = 3.7 PMSYY78 pKa = 10.31 QYY80 pKa = 11.58 LVGAEE85 pKa = 3.64 IDD87 pKa = 3.75 YY88 pKa = 10.77 TEE90 pKa = 4.43 GLEE93 pKa = 4.39 GSQFVIKK100 pKa = 10.6 NPNATSTCGCGSSFSAA116 pKa = 4.79
Molecular weight: 12.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 4.05
IPC_protein 3.948
Toseland 3.757
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.681
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.19
Thurlkill 3.808
EMBOSS 3.834
Sillero 4.062
Patrickios 1.901
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.95
Protein with the highest isoelectric point:
>tr|A0A497XPL0|A0A497XPL0_9PROT Uncharacterized protein involved in tolerance to divalent cations OS=Sulfurisoma sediminicola OX=1381557 GN=DFR35_0643 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.6 RR14 pKa = 11.84 THH16 pKa = 5.72 GFLVRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 HH40 pKa = 4.92 RR41 pKa = 11.84 LAVV44 pKa = 3.37
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.461
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2823
0
2823
922662
28
8624
326.8
35.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.906 ± 0.072
0.997 ± 0.017
5.473 ± 0.045
5.804 ± 0.053
3.719 ± 0.029
8.475 ± 0.063
2.26 ± 0.031
4.777 ± 0.034
3.718 ± 0.056
10.746 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.417 ± 0.024
2.581 ± 0.039
5.067 ± 0.034
3.234 ± 0.027
7.026 ± 0.063
4.74 ± 0.053
4.891 ± 0.065
7.432 ± 0.036
1.421 ± 0.02
2.318 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here