Streptococcus phage SW3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S5H0Q0|A0A3S5H0Q0_9CAUD Head-tail connector protein OS=Streptococcus phage SW3 OX=2419649 GN=SW3_011 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.65IEE28 pKa = 3.9SARR31 pKa = 11.84QYY33 pKa = 10.3VVNSVGDD40 pKa = 3.54DD41 pKa = 3.37PKK43 pKa = 10.95FYY45 pKa = 11.07NLDD48 pKa = 3.41SVRR51 pKa = 11.84ALFDD55 pKa = 3.34TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.66ATYY98 pKa = 10.56SEE100 pKa = 4.46EE101 pKa = 5.06RR102 pKa = 11.84GDD104 pKa = 3.53

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7W8S1|A0A3S7W8S1_9CAUD Uncharacterized protein OS=Streptococcus phage SW3 OX=2419649 GN=SW3_042 PE=4 SV=1
MM1 pKa = 7.43EE2 pKa = 5.84RR3 pKa = 11.84LLKK6 pKa = 9.63RR7 pKa = 11.84TTYY10 pKa = 10.7RR11 pKa = 11.84EE12 pKa = 4.1LNVAIALLLASLIINIGTLVSVVNRR37 pKa = 11.84PLKK40 pKa = 10.28PIIVYY45 pKa = 10.1KK46 pKa = 10.54ADD48 pKa = 3.41NSAVMHH54 pKa = 6.02GKK56 pKa = 8.14ITGKK60 pKa = 10.18QMIGKK65 pKa = 9.8LYY67 pKa = 9.98TLDD70 pKa = 3.56CGAYY74 pKa = 10.2GKK76 pKa = 10.61FLVTKK81 pKa = 9.1EE82 pKa = 3.96QYY84 pKa = 10.91NSVNVGDD91 pKa = 5.86DD92 pKa = 2.98IPNYY96 pKa = 10.6LKK98 pKa = 11.04GRR100 pKa = 11.84GNN102 pKa = 3.45

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

11190

48

1591

248.7

28.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.524 ± 0.529

0.554 ± 0.133

6.434 ± 0.301

6.72 ± 0.462

4.441 ± 0.218

6.443 ± 0.479

1.448 ± 0.104

6.318 ± 0.228

8.588 ± 0.479

8.105 ± 0.373

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.52 ± 0.169

6.443 ± 0.27

2.949 ± 0.188

4.361 ± 0.247

3.789 ± 0.291

6.479 ± 0.314

6.55 ± 0.337

5.791 ± 0.214

1.448 ± 0.15

4.093 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski