Amino acid dipepetide frequency for Streptococcus phage SW3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.771AlaAla: 2.771 ± 0.975
0.268AlaCys: 0.268 ± 0.158
4.201AlaAsp: 4.201 ± 0.601
3.486AlaGlu: 3.486 ± 0.447
2.771AlaPhe: 2.771 ± 0.531
3.843AlaGly: 3.843 ± 0.726
0.804AlaHis: 0.804 ± 0.291
4.022AlaIle: 4.022 ± 0.744
5.809AlaLys: 5.809 ± 0.952
6.435AlaLeu: 6.435 ± 0.715
1.877AlaMet: 1.877 ± 0.431
4.826AlaAsn: 4.826 ± 0.699
1.519AlaPro: 1.519 ± 0.38
3.039AlaGln: 3.039 ± 0.596
2.681AlaArg: 2.681 ± 0.477
4.916AlaSer: 4.916 ± 0.73
3.932AlaThr: 3.932 ± 0.808
4.469AlaVal: 4.469 ± 0.709
1.162AlaTrp: 1.162 ± 0.339
2.413AlaTyr: 2.413 ± 0.352
0.0AlaXaa: 0.0 ± 0.0
Cys
0.179CysAla: 0.179 ± 0.127
0.089CysCys: 0.089 ± 0.112
0.894CysAsp: 0.894 ± 0.299
0.536CysGlu: 0.536 ± 0.315
0.626CysPhe: 0.626 ± 0.246
0.179CysGly: 0.179 ± 0.113
0.089CysHis: 0.089 ± 0.093
0.0CysIle: 0.0 ± 0.0
0.357CysLys: 0.357 ± 0.195
0.357CysLeu: 0.357 ± 0.184
0.089CysMet: 0.089 ± 0.091
0.089CysAsn: 0.089 ± 0.073
0.179CysPro: 0.179 ± 0.128
0.089CysGln: 0.089 ± 0.102
0.268CysArg: 0.268 ± 0.231
0.536CysSer: 0.536 ± 0.259
0.357CysThr: 0.357 ± 0.194
0.268CysVal: 0.268 ± 0.133
0.179CysTrp: 0.179 ± 0.112
0.179CysTyr: 0.179 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
4.022AspAla: 4.022 ± 0.615
0.626AspCys: 0.626 ± 0.221
4.201AspAsp: 4.201 ± 0.839
4.647AspGlu: 4.647 ± 0.58
3.754AspPhe: 3.754 ± 0.723
5.72AspGly: 5.72 ± 0.976
1.072AspHis: 1.072 ± 0.284
5.631AspIle: 5.631 ± 0.801
4.558AspLys: 4.558 ± 0.689
4.916AspLeu: 4.916 ± 0.892
2.056AspMet: 2.056 ± 0.452
4.469AspAsn: 4.469 ± 0.752
2.324AspPro: 2.324 ± 0.387
1.341AspGln: 1.341 ± 0.351
2.056AspArg: 2.056 ± 0.546
3.217AspSer: 3.217 ± 0.509
3.932AspThr: 3.932 ± 0.622
2.592AspVal: 2.592 ± 0.616
0.626AspTrp: 0.626 ± 0.206
2.592AspTyr: 2.592 ± 0.447
0.0AspXaa: 0.0 ± 0.0
Glu
4.737GluAla: 4.737 ± 0.51
0.357GluCys: 0.357 ± 0.189
3.486GluAsp: 3.486 ± 0.645
4.558GluGlu: 4.558 ± 0.772
2.413GluPhe: 2.413 ± 0.537
2.681GluGly: 2.681 ± 0.358
1.341GluHis: 1.341 ± 0.33
4.826GluIle: 4.826 ± 0.704
4.737GluLys: 4.737 ± 0.867
6.614GluLeu: 6.614 ± 0.557
2.681GluMet: 2.681 ± 0.55
3.754GluAsn: 3.754 ± 0.582
1.698GluPro: 1.698 ± 0.387
3.217GluGln: 3.217 ± 0.604
3.217GluArg: 3.217 ± 0.571
3.575GluSer: 3.575 ± 0.623
4.022GluThr: 4.022 ± 0.613
5.362GluVal: 5.362 ± 0.771
1.072GluTrp: 1.072 ± 0.346
2.86GluTyr: 2.86 ± 0.468
0.0GluXaa: 0.0 ± 0.0
Phe
3.396PheAla: 3.396 ± 0.498
0.179PheCys: 0.179 ± 0.175
3.217PheAsp: 3.217 ± 0.495
2.681PheGlu: 2.681 ± 0.579
2.234PhePhe: 2.234 ± 0.377
3.039PheGly: 3.039 ± 0.727
0.626PheHis: 0.626 ± 0.199
2.681PheIle: 2.681 ± 0.458
5.005PheLys: 5.005 ± 0.763
2.949PheLeu: 2.949 ± 0.451
0.536PheMet: 0.536 ± 0.213
3.843PheAsn: 3.843 ± 0.762
0.804PhePro: 0.804 ± 0.298
1.341PheGln: 1.341 ± 0.32
0.894PheArg: 0.894 ± 0.251
2.86PheSer: 2.86 ± 0.578
2.949PheThr: 2.949 ± 0.558
2.324PheVal: 2.324 ± 0.407
0.715PheTrp: 0.715 ± 0.224
2.145PheTyr: 2.145 ± 0.399
0.0PheXaa: 0.0 ± 0.0
Gly
2.949GlyAla: 2.949 ± 0.73
0.447GlyCys: 0.447 ± 0.214
4.022GlyAsp: 4.022 ± 0.421
3.486GlyGlu: 3.486 ± 0.577
3.575GlyPhe: 3.575 ± 0.514
4.469GlyGly: 4.469 ± 0.797
1.072GlyHis: 1.072 ± 0.253
5.094GlyIle: 5.094 ± 0.704
5.988GlyLys: 5.988 ± 0.765
5.273GlyLeu: 5.273 ± 0.844
1.162GlyMet: 1.162 ± 0.328
3.932GlyAsn: 3.932 ± 0.708
1.251GlyPro: 1.251 ± 0.318
3.039GlyGln: 3.039 ± 0.549
2.592GlyArg: 2.592 ± 0.403
4.647GlySer: 4.647 ± 0.709
4.469GlyThr: 4.469 ± 0.613
2.949GlyVal: 2.949 ± 0.637
0.983GlyTrp: 0.983 ± 0.341
3.039GlyTyr: 3.039 ± 0.565
0.0GlyXaa: 0.0 ± 0.0
His
0.626HisAla: 0.626 ± 0.219
0.089HisCys: 0.089 ± 0.079
0.626HisAsp: 0.626 ± 0.176
0.447HisGlu: 0.447 ± 0.199
0.715HisPhe: 0.715 ± 0.258
0.715HisGly: 0.715 ± 0.204
0.357HisHis: 0.357 ± 0.138
1.072HisIle: 1.072 ± 0.312
1.251HisLys: 1.251 ± 0.384
1.43HisLeu: 1.43 ± 0.335
0.357HisMet: 0.357 ± 0.152
0.804HisAsn: 0.804 ± 0.333
0.536HisPro: 0.536 ± 0.178
0.536HisGln: 0.536 ± 0.233
0.715HisArg: 0.715 ± 0.215
0.804HisSer: 0.804 ± 0.24
0.894HisThr: 0.894 ± 0.272
1.519HisVal: 1.519 ± 0.295
0.179HisTrp: 0.179 ± 0.133
0.804HisTyr: 0.804 ± 0.328
0.0HisXaa: 0.0 ± 0.0
Ile
5.273IleAla: 5.273 ± 0.845
0.357IleCys: 0.357 ± 0.18
4.737IleAsp: 4.737 ± 0.615
4.379IleGlu: 4.379 ± 0.741
1.251IlePhe: 1.251 ± 0.34
4.916IleGly: 4.916 ± 0.539
0.536IleHis: 0.536 ± 0.233
3.128IleIle: 3.128 ± 0.624
5.809IleLys: 5.809 ± 0.734
3.754IleLeu: 3.754 ± 0.669
1.966IleMet: 1.966 ± 0.44
4.826IleAsn: 4.826 ± 0.713
2.502IlePro: 2.502 ± 0.413
2.949IleGln: 2.949 ± 0.421
2.413IleArg: 2.413 ± 0.456
3.664IleSer: 3.664 ± 0.622
4.558IleThr: 4.558 ± 0.564
3.486IleVal: 3.486 ± 0.606
0.715IleTrp: 0.715 ± 0.246
1.966IleTyr: 1.966 ± 0.503
0.0IleXaa: 0.0 ± 0.0
Lys
5.72LysAla: 5.72 ± 0.644
0.357LysCys: 0.357 ± 0.206
5.541LysAsp: 5.541 ± 0.703
7.597LysGlu: 7.597 ± 0.97
4.379LysPhe: 4.379 ± 0.756
5.094LysGly: 5.094 ± 0.803
1.698LysHis: 1.698 ± 0.492
5.273LysIle: 5.273 ± 0.617
6.346LysLys: 6.346 ± 1.128
6.971LysLeu: 6.971 ± 0.846
2.056LysMet: 2.056 ± 0.422
5.541LysAsn: 5.541 ± 0.716
3.396LysPro: 3.396 ± 0.414
4.201LysGln: 4.201 ± 0.447
3.128LysArg: 3.128 ± 0.511
4.558LysSer: 4.558 ± 0.568
5.541LysThr: 5.541 ± 0.91
3.932LysVal: 3.932 ± 0.681
1.162LysTrp: 1.162 ± 0.287
3.396LysTyr: 3.396 ± 0.588
0.0LysXaa: 0.0 ± 0.0
Leu
6.703LeuAla: 6.703 ± 0.797
0.447LeuCys: 0.447 ± 0.182
5.988LeuAsp: 5.988 ± 0.749
6.703LeuGlu: 6.703 ± 0.947
3.039LeuPhe: 3.039 ± 0.395
5.631LeuGly: 5.631 ± 0.953
1.072LeuHis: 1.072 ± 0.349
4.469LeuIle: 4.469 ± 0.606
7.15LeuLys: 7.15 ± 0.72
5.72LeuLeu: 5.72 ± 0.778
2.86LeuMet: 2.86 ± 0.461
5.273LeuAsn: 5.273 ± 0.722
2.681LeuPro: 2.681 ± 0.391
2.324LeuGln: 2.324 ± 0.489
2.949LeuArg: 2.949 ± 0.713
4.737LeuSer: 4.737 ± 0.594
6.346LeuThr: 6.346 ± 0.961
4.29LeuVal: 4.29 ± 0.509
0.715LeuTrp: 0.715 ± 0.206
1.966LeuTyr: 1.966 ± 0.497
0.0LeuXaa: 0.0 ± 0.0
Met
2.056MetAla: 2.056 ± 0.41
0.0MetCys: 0.0 ± 0.0
1.251MetAsp: 1.251 ± 0.299
1.251MetGlu: 1.251 ± 0.439
1.43MetPhe: 1.43 ± 0.285
0.804MetGly: 0.804 ± 0.315
0.268MetHis: 0.268 ± 0.161
2.056MetIle: 2.056 ± 0.448
3.128MetLys: 3.128 ± 0.616
2.145MetLeu: 2.145 ± 0.329
0.804MetMet: 0.804 ± 0.251
0.715MetAsn: 0.715 ± 0.257
0.804MetPro: 0.804 ± 0.192
0.804MetGln: 0.804 ± 0.242
0.626MetArg: 0.626 ± 0.216
2.592MetSer: 2.592 ± 0.367
1.787MetThr: 1.787 ± 0.507
1.341MetVal: 1.341 ± 0.393
0.179MetTrp: 0.179 ± 0.107
1.162MetTyr: 1.162 ± 0.296
0.0MetXaa: 0.0 ± 0.0
Asn
4.469AsnAla: 4.469 ± 1.083
0.357AsnCys: 0.357 ± 0.182
3.664AsnAsp: 3.664 ± 0.62
4.379AsnGlu: 4.379 ± 0.72
2.592AsnPhe: 2.592 ± 0.524
6.882AsnGly: 6.882 ± 1.137
0.715AsnHis: 0.715 ± 0.212
3.396AsnIle: 3.396 ± 0.518
4.558AsnLys: 4.558 ± 0.761
4.737AsnLeu: 4.737 ± 0.578
0.983AsnMet: 0.983 ± 0.262
4.647AsnAsn: 4.647 ± 0.81
2.681AsnPro: 2.681 ± 0.523
3.039AsnGln: 3.039 ± 0.451
2.324AsnArg: 2.324 ± 0.51
3.843AsnSer: 3.843 ± 0.666
3.575AsnThr: 3.575 ± 0.64
3.486AsnVal: 3.486 ± 0.616
1.251AsnTrp: 1.251 ± 0.335
2.771AsnTyr: 2.771 ± 0.623
0.0AsnXaa: 0.0 ± 0.0
Pro
1.698ProAla: 1.698 ± 0.349
0.089ProCys: 0.089 ± 0.103
1.519ProAsp: 1.519 ± 0.394
2.413ProGlu: 2.413 ± 0.475
1.162ProPhe: 1.162 ± 0.295
0.894ProGly: 0.894 ± 0.306
0.536ProHis: 0.536 ± 0.218
1.43ProIle: 1.43 ± 0.345
3.575ProLys: 3.575 ± 0.473
2.413ProLeu: 2.413 ± 0.368
0.268ProMet: 0.268 ± 0.185
2.592ProAsn: 2.592 ± 0.451
0.447ProPro: 0.447 ± 0.246
1.519ProGln: 1.519 ± 0.265
0.983ProArg: 0.983 ± 0.313
2.502ProSer: 2.502 ± 0.527
2.056ProThr: 2.056 ± 0.34
1.787ProVal: 1.787 ± 0.352
0.536ProTrp: 0.536 ± 0.176
1.072ProTyr: 1.072 ± 0.37
0.0ProXaa: 0.0 ± 0.0
Gln
3.664GlnAla: 3.664 ± 0.5
0.179GlnCys: 0.179 ± 0.116
2.502GlnAsp: 2.502 ± 0.5
3.754GlnGlu: 3.754 ± 0.611
1.609GlnPhe: 1.609 ± 0.521
3.039GlnGly: 3.039 ± 0.605
0.179GlnHis: 0.179 ± 0.127
2.234GlnIle: 2.234 ± 0.483
3.307GlnLys: 3.307 ± 0.624
3.754GlnLeu: 3.754 ± 0.477
1.251GlnMet: 1.251 ± 0.293
2.771GlnAsn: 2.771 ± 0.438
0.626GlnPro: 0.626 ± 0.222
3.486GlnGln: 3.486 ± 0.727
1.698GlnArg: 1.698 ± 0.335
2.145GlnSer: 2.145 ± 0.407
2.681GlnThr: 2.681 ± 0.409
2.056GlnVal: 2.056 ± 0.541
0.894GlnTrp: 0.894 ± 0.297
1.787GlnTyr: 1.787 ± 0.361
0.0GlnXaa: 0.0 ± 0.0
Arg
2.592ArgAla: 2.592 ± 0.458
0.089ArgCys: 0.089 ± 0.102
2.234ArgAsp: 2.234 ± 0.526
2.413ArgGlu: 2.413 ± 0.539
2.324ArgPhe: 2.324 ± 0.43
2.145ArgGly: 2.145 ± 0.492
0.536ArgHis: 0.536 ± 0.204
2.592ArgIle: 2.592 ± 0.604
3.307ArgLys: 3.307 ± 0.68
3.843ArgLeu: 3.843 ± 0.666
0.804ArgMet: 0.804 ± 0.285
1.877ArgAsn: 1.877 ± 0.344
0.715ArgPro: 0.715 ± 0.253
2.592ArgGln: 2.592 ± 0.541
1.072ArgArg: 1.072 ± 0.288
1.609ArgSer: 1.609 ± 0.408
2.145ArgThr: 2.145 ± 0.641
2.592ArgVal: 2.592 ± 0.398
0.536ArgTrp: 0.536 ± 0.156
1.877ArgTyr: 1.877 ± 0.509
0.0ArgXaa: 0.0 ± 0.0
Ser
3.754SerAla: 3.754 ± 0.601
0.447SerCys: 0.447 ± 0.217
4.29SerAsp: 4.29 ± 0.47
3.396SerGlu: 3.396 ± 0.527
2.86SerPhe: 2.86 ± 0.409
3.486SerGly: 3.486 ± 0.6
0.626SerHis: 0.626 ± 0.218
4.201SerIle: 4.201 ± 0.491
5.809SerLys: 5.809 ± 0.745
4.916SerLeu: 4.916 ± 0.636
1.698SerMet: 1.698 ± 0.365
4.111SerAsn: 4.111 ± 0.685
2.413SerPro: 2.413 ± 0.443
2.771SerGln: 2.771 ± 0.468
2.949SerArg: 2.949 ± 0.696
4.558SerSer: 4.558 ± 1.171
4.647SerThr: 4.647 ± 0.685
4.29SerVal: 4.29 ± 0.715
0.983SerTrp: 0.983 ± 0.405
2.592SerTyr: 2.592 ± 0.52
0.0SerXaa: 0.0 ± 0.0
Thr
4.469ThrAla: 4.469 ± 0.712
0.268ThrCys: 0.268 ± 0.143
4.111ThrAsp: 4.111 ± 0.668
4.201ThrGlu: 4.201 ± 0.606
2.502ThrPhe: 2.502 ± 0.506
4.022ThrGly: 4.022 ± 0.451
0.983ThrHis: 0.983 ± 0.278
4.022ThrIle: 4.022 ± 0.745
5.988ThrLys: 5.988 ± 0.794
6.524ThrLeu: 6.524 ± 0.748
1.072ThrMet: 1.072 ± 0.266
4.022ThrAsn: 4.022 ± 0.572
1.609ThrPro: 1.609 ± 0.469
2.949ThrGln: 2.949 ± 0.555
1.787ThrArg: 1.787 ± 0.373
4.29ThrSer: 4.29 ± 0.714
3.843ThrThr: 3.843 ± 0.767
3.932ThrVal: 3.932 ± 0.562
1.251ThrTrp: 1.251 ± 0.393
3.664ThrTyr: 3.664 ± 0.619
0.0ThrXaa: 0.0 ± 0.0
Val
3.396ValAla: 3.396 ± 0.73
0.268ValCys: 0.268 ± 0.138
4.379ValAsp: 4.379 ± 0.386
3.575ValGlu: 3.575 ± 0.518
2.056ValPhe: 2.056 ± 0.394
3.575ValGly: 3.575 ± 0.532
0.626ValHis: 0.626 ± 0.207
4.111ValIle: 4.111 ± 0.629
5.273ValLys: 5.273 ± 0.587
3.664ValLeu: 3.664 ± 0.773
0.894ValMet: 0.894 ± 0.309
3.843ValAsn: 3.843 ± 0.76
1.609ValPro: 1.609 ± 0.39
1.609ValGln: 1.609 ± 0.337
2.413ValArg: 2.413 ± 0.658
5.005ValSer: 5.005 ± 0.703
4.737ValThr: 4.737 ± 0.772
3.664ValVal: 3.664 ± 0.627
1.072ValTrp: 1.072 ± 0.272
2.145ValTyr: 2.145 ± 0.473
0.0ValXaa: 0.0 ± 0.0
Trp
0.715TrpAla: 0.715 ± 0.282
0.0TrpCys: 0.0 ± 0.0
1.162TrpAsp: 1.162 ± 0.383
0.626TrpGlu: 0.626 ± 0.211
0.536TrpPhe: 0.536 ± 0.192
0.536TrpGly: 0.536 ± 0.184
0.357TrpHis: 0.357 ± 0.2
0.894TrpIle: 0.894 ± 0.285
0.804TrpLys: 0.804 ± 0.279
1.609TrpLeu: 1.609 ± 0.324
0.089TrpMet: 0.089 ± 0.095
0.804TrpAsn: 0.804 ± 0.285
0.268TrpPro: 0.268 ± 0.181
0.536TrpGln: 0.536 ± 0.199
0.894TrpArg: 0.894 ± 0.263
1.877TrpSer: 1.877 ± 0.602
0.894TrpThr: 0.894 ± 0.207
1.162TrpVal: 1.162 ± 0.325
0.268TrpTrp: 0.268 ± 0.134
0.447TrpTyr: 0.447 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.056TyrAla: 2.056 ± 0.37
0.626TyrCys: 0.626 ± 0.292
2.324TyrAsp: 2.324 ± 0.38
2.681TyrGlu: 2.681 ± 0.494
2.592TyrPhe: 2.592 ± 0.426
2.771TyrGly: 2.771 ± 0.448
0.983TyrHis: 0.983 ± 0.305
2.056TyrIle: 2.056 ± 0.364
3.128TyrLys: 3.128 ± 0.468
3.039TyrLeu: 3.039 ± 0.488
1.609TyrMet: 1.609 ± 0.445
1.698TyrAsn: 1.698 ± 0.335
1.43TyrPro: 1.43 ± 0.382
2.234TyrGln: 2.234 ± 0.4
2.234TyrArg: 2.234 ± 0.422
2.86TyrSer: 2.86 ± 0.549
2.145TyrThr: 2.145 ± 0.493
2.413TyrVal: 2.413 ± 0.419
0.0TyrTrp: 0.0 ± 0.0
2.056TyrTyr: 2.056 ± 0.63
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (11190 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski