Roseibaca ekhonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Roseibaca

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B0MBM0|A0A3B0MBM0_9RHOB Tetrathionate reductase subunit B OS=Roseibaca ekhonensis OX=254356 GN=ttrB PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.11LKK4 pKa = 10.61HH5 pKa = 5.66LAAYY9 pKa = 10.1SLAAQSLALGTAFAQTPLTMWYY31 pKa = 9.31HH32 pKa = 5.69GAGNEE37 pKa = 4.07VEE39 pKa = 4.39SAIINQIVDD48 pKa = 4.35DD49 pKa = 4.79FNASQSDD56 pKa = 3.32WSVSLEE62 pKa = 4.06SFPQGAYY69 pKa = 9.97NDD71 pKa = 4.3SVVAAALAGNLPDD84 pKa = 4.5ILDD87 pKa = 3.29VDD89 pKa = 4.65GPVMPNWAWAGYY101 pKa = 7.25MQPLPLDD108 pKa = 3.6EE109 pKa = 5.35SVIADD114 pKa = 4.35FLPGTKK120 pKa = 10.12GYY122 pKa = 9.82WDD124 pKa = 3.95GEE126 pKa = 4.42LYY128 pKa = 10.91SIGLWDD134 pKa = 3.9AAVAMVARR142 pKa = 11.84QSTLDD147 pKa = 3.55ALGLRR152 pKa = 11.84TPTVAEE158 pKa = 4.09PWTGEE163 pKa = 3.9EE164 pKa = 5.13FMAALDD170 pKa = 3.84AAKK173 pKa = 9.37ATGDD177 pKa = 3.09WEE179 pKa = 4.52YY180 pKa = 11.73AFDD183 pKa = 5.96PGMAWTGEE191 pKa = 3.93WYY193 pKa = 9.24PYY195 pKa = 10.83AFSPFLQSFGGDD207 pKa = 2.53IVDD210 pKa = 3.7RR211 pKa = 11.84STYY214 pKa = 10.23QSAEE218 pKa = 3.73GVLNGDD224 pKa = 3.63AAIEE228 pKa = 4.07FGEE231 pKa = 4.1WWQSLFAEE239 pKa = 6.43GYY241 pKa = 10.97APGTSQDD248 pKa = 3.0PAARR252 pKa = 11.84DD253 pKa = 3.4NGFLEE258 pKa = 4.15GRR260 pKa = 11.84YY261 pKa = 9.68AFSWNGNWAALNALNAFDD279 pKa = 4.85DD280 pKa = 4.34TLFLPAPDD288 pKa = 4.62FGNGPKK294 pKa = 9.72IGAASWQFGVSGSSDD309 pKa = 3.6HH310 pKa = 7.41PDD312 pKa = 2.96GAAAFIEE319 pKa = 4.67FALQDD324 pKa = 3.57EE325 pKa = 4.68YY326 pKa = 11.78LAAFSNGIGLIPATASAAAMTEE348 pKa = 4.01NYY350 pKa = 10.83AEE352 pKa = 4.53GGAMAEE358 pKa = 3.99FFEE361 pKa = 5.27LSEE364 pKa = 4.21AQALVRR370 pKa = 11.84PVTPGYY376 pKa = 10.01VVQAKK381 pKa = 9.65VFEE384 pKa = 4.37KK385 pKa = 10.94ALADD389 pKa = 3.53IANGADD395 pKa = 3.38VADD398 pKa = 4.21TLDD401 pKa = 3.73AAVDD405 pKa = 4.31EE406 pKa = 4.55IDD408 pKa = 5.07ADD410 pKa = 3.63IEE412 pKa = 4.36RR413 pKa = 11.84NSGYY417 pKa = 11.02GHH419 pKa = 7.45

Molecular weight: 44.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B0M734|A0A3B0M734_9RHOB Membrane-bound lytic murein transglycosylase B OS=Roseibaca ekhonensis OX=254356 GN=mltB_3 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.83HH14 pKa = 4.64RR15 pKa = 11.84HH16 pKa = 3.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.77IINARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84MGRR39 pKa = 11.84KK40 pKa = 9.27KK41 pKa = 9.66LTAA44 pKa = 4.18

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3887

0

3887

1169467

30

2469

300.9

32.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.972 ± 0.057

0.921 ± 0.014

6.289 ± 0.032

5.462 ± 0.039

3.638 ± 0.027

8.557 ± 0.037

2.146 ± 0.023

4.797 ± 0.029

2.79 ± 0.036

10.307 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.02

2.436 ± 0.021

5.207 ± 0.029

3.405 ± 0.022

7.206 ± 0.035

4.981 ± 0.025

5.446 ± 0.027

7.075 ± 0.034

1.448 ± 0.017

2.105 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski