Sphingomonas sp. HDW15A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7ZJK4|A0A6G7ZJK4_9SPHN DNA polymerase Y family protein OS=Sphingomonas sp. HDW15A OX=2714942 GN=G7076_00005 PE=3 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84TFSRR6 pKa = 11.84RR7 pKa = 11.84NRR9 pKa = 11.84AACSILAMATVFTVGATPAAAQVAGTGNFSTGTGTINPGAGTVDD53 pKa = 3.03ITGGGQAVIDD63 pKa = 3.89WNATGTVVGGVNQFLAPGNTLTFSSSSDD91 pKa = 3.25FAVLNNVSTGTDD103 pKa = 3.15MLGLNGTVTSSVDD116 pKa = 3.28GVQGRR121 pKa = 11.84GSVYY125 pKa = 9.91FYY127 pKa = 10.59NPNGIVVGGSSVFNVGSLVLSTSPILVSGGAFIDD161 pKa = 4.3GNNQVVFQQGLAGASIDD178 pKa = 3.94VQSGAQINATSNNSYY193 pKa = 10.28VALVAPRR200 pKa = 11.84VVHH203 pKa = 6.63NGTINVNGSAALVGAEE219 pKa = 4.1AANITFSPDD228 pKa = 2.72GLFDD232 pKa = 3.75IEE234 pKa = 4.43VTAGTTDD241 pKa = 3.13ANGVVIGGTITGPEE255 pKa = 3.93AGVGEE260 pKa = 4.41TQRR263 pKa = 11.84IYY265 pKa = 11.39AVAVPKK271 pKa = 10.06NQAMTLAITAGAQLGFDD288 pKa = 2.97IAGAVNVDD296 pKa = 3.05GNAIVLSAGRR306 pKa = 11.84NVTNGDD312 pKa = 3.29ADD314 pKa = 4.1TARR317 pKa = 11.84SAAMGSGTASINSNDD332 pKa = 3.62LTTTSAITASAVNNNIFLGFNDD354 pKa = 3.16ITMASDD360 pKa = 3.25VTIIGEE366 pKa = 4.26DD367 pKa = 3.55MVHH370 pKa = 6.29LRR372 pKa = 11.84AIGANADD379 pKa = 3.77LTVGGLLTMSGDD391 pKa = 3.7VFVAAGQAGQAGDD404 pKa = 3.57VMVSAEE410 pKa = 4.12GGALLTASGGAVLSAVARR428 pKa = 11.84GQASGTAGVNGGNAQGGDD446 pKa = 3.51INVLGSDD453 pKa = 3.74GTLVIAGDD461 pKa = 4.05LSVDD465 pKa = 3.07ANAYY469 pKa = 10.06GGEE472 pKa = 4.14TLVNGANAGSAVGGLIAITAYY493 pKa = 10.5GPDD496 pKa = 3.47GTLSINGGLFATADD510 pKa = 3.72GVGGNGGYY518 pKa = 9.47SEE520 pKa = 5.43SCFSCNGAGGNGQGGQIYY538 pKa = 8.89FQTVGNGGFLTIGDD552 pKa = 5.31DD553 pKa = 3.68IQASASGSGGSSRR566 pKa = 11.84TATGRR571 pKa = 11.84GDD573 pKa = 3.4GGTISATYY581 pKa = 8.99GANGTIQFDD590 pKa = 3.91ANASFTATGAGGQSDD605 pKa = 4.13ALGVDD610 pKa = 3.78GGYY613 pKa = 11.21GKK615 pKa = 11.06GGTVKK620 pKa = 10.72FGAQNNATGGSLTVAGLAYY639 pKa = 10.2AAIEE643 pKa = 4.78GYY645 pKa = 10.88GGDD648 pKa = 3.61SYY650 pKa = 12.04GAGTGNGGTGEE661 pKa = 4.84GGLSDD666 pKa = 3.46NGGNAGTINFQGGLQILGRR685 pKa = 11.84GFGGDD690 pKa = 3.65GNNGTGGQGLGGQGWIDD707 pKa = 3.27AFGANVTIGGDD718 pKa = 3.31YY719 pKa = 10.5DD720 pKa = 4.21INVSGLGGSGITGGFGRR737 pKa = 11.84GGNAYY742 pKa = 9.48LASSGTFLLTGNADD756 pKa = 3.56LVADD760 pKa = 4.25GRR762 pKa = 11.84GGGAFGGNGGLGQGGLANVRR782 pKa = 11.84ATGSGTLTVQGNVDD796 pKa = 3.23AHH798 pKa = 5.97ATGQGGFVAFGSGTAGDD815 pKa = 3.95GDD817 pKa = 3.94GGVVSIHH824 pKa = 6.71AGNGGATPSGGTLNILGSLTGVSDD848 pKa = 5.01GIGGLADD855 pKa = 3.48TATSVGGTGRR865 pKa = 11.84NAEE868 pKa = 4.45LFLFAAGGGDD878 pKa = 3.66LSVGGSTDD886 pKa = 3.45LSADD890 pKa = 3.47GLAGFYY896 pKa = 10.45LEE898 pKa = 5.74CLDD901 pKa = 4.96CGGTPGIGDD910 pKa = 4.03GGNLNISADD919 pKa = 3.9GAGSTLSFGDD929 pKa = 4.67DD930 pKa = 3.34LTATADD936 pKa = 3.52GRR938 pKa = 11.84GGLSSTDD945 pKa = 3.41GGTGGYY951 pKa = 10.58GEE953 pKa = 5.01GGNAGMFAGNGGTITATYY971 pKa = 10.52VDD973 pKa = 4.12ISAIGTGGFDD983 pKa = 3.71YY984 pKa = 11.33NGVGGGAGKK993 pKa = 10.55GGNVYY998 pKa = 10.43FNTNATGVNTITVTGQVFLDD1018 pKa = 3.39ASGYY1022 pKa = 10.8GGGSYY1027 pKa = 10.36GGNGGAGTGGVAGMFVQTGTVTLGSADD1054 pKa = 3.0ISATGEE1060 pKa = 3.98GGTVIEE1066 pKa = 4.55GTGTGGDD1073 pKa = 3.49ATGGSARR1080 pKa = 11.84LQGAGGDD1087 pKa = 3.48ILIAGNLAMDD1097 pKa = 4.53GSAIGGNGTAGGNAIALADD1116 pKa = 3.71LTVNEE1121 pKa = 4.62TNPIAVGVYY1130 pKa = 10.01ANNNGSVTVNGAVAIDD1146 pKa = 3.99ASATGGNGSAGNGGNATAGEE1166 pKa = 4.06IDD1168 pKa = 3.13IVAFFGDD1175 pKa = 3.43IAMGDD1180 pKa = 3.83LAVDD1184 pKa = 3.63ISGNGGDD1191 pKa = 4.52GGQGGNGGNATGGYY1205 pKa = 10.53ADD1207 pKa = 3.52IAFGLGAAAIGGTLTLASATVVADD1231 pKa = 4.67GIGGDD1236 pKa = 3.87GGAGVDD1242 pKa = 4.42GNTGGNGGNGGTGTGGRR1259 pKa = 11.84ISFVGSAAGGFLTSGITVLSAGATGGDD1286 pKa = 3.52GGVGGNGDD1294 pKa = 3.84TGTGGNGGTGGAAFSGSIQSGTVSASGAPGSGGGFDD1330 pKa = 3.64VTSLFAATNAAGGGAGRR1347 pKa = 11.84PGLAAAATEE1356 pKa = 4.39TAATAAVRR1364 pKa = 11.84KK1365 pKa = 9.71VDD1367 pKa = 3.78RR1368 pKa = 11.84QASS1371 pKa = 3.49

Molecular weight: 127.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7ZH44|A0A6G7ZH44_9SPHN Glycine cleavage system T protein OS=Sphingomonas sp. HDW15A OX=2714942 GN=gcvT PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2226

0

2226

649559

41

1809

291.8

31.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.77 ± 0.081

0.78 ± 0.016

5.9 ± 0.037

5.966 ± 0.051

3.546 ± 0.037

8.812 ± 0.064

1.881 ± 0.024

5.14 ± 0.039

3.481 ± 0.042

9.924 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.026

2.607 ± 0.036

5.18 ± 0.037

3.004 ± 0.028

7.354 ± 0.062

5.559 ± 0.039

5.018 ± 0.049

7.141 ± 0.047

1.411 ± 0.026

2.137 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski