Amino acid dipepetide frequency for Sphingomonas sp. HDW15A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.009AlaAla: 18.009 ± 0.229
0.985AlaCys: 0.985 ± 0.044
7.177AlaAsp: 7.177 ± 0.112
8.118AlaGlu: 8.118 ± 0.14
4.395AlaPhe: 4.395 ± 0.091
10.717AlaGly: 10.717 ± 0.175
2.075AlaHis: 2.075 ± 0.064
7.126AlaIle: 7.126 ± 0.13
4.657AlaLys: 4.657 ± 0.108
13.437AlaLeu: 13.437 ± 0.184
3.935AlaMet: 3.935 ± 0.094
3.279AlaAsn: 3.279 ± 0.084
5.55AlaPro: 5.55 ± 0.111
3.901AlaGln: 3.901 ± 0.085
8.925AlaArg: 8.925 ± 0.143
6.671AlaSer: 6.671 ± 0.097
6.232AlaThr: 6.232 ± 0.111
8.366AlaVal: 8.366 ± 0.112
1.569AlaTrp: 1.569 ± 0.055
2.577AlaTyr: 2.577 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.93CysAla: 0.93 ± 0.043
0.091CysCys: 0.091 ± 0.013
0.527CysAsp: 0.527 ± 0.028
0.47CysGlu: 0.47 ± 0.028
0.299CysPhe: 0.299 ± 0.02
0.904CysGly: 0.904 ± 0.042
0.229CysHis: 0.229 ± 0.019
0.354CysIle: 0.354 ± 0.025
0.185CysLys: 0.185 ± 0.019
0.682CysLeu: 0.682 ± 0.036
0.12CysMet: 0.12 ± 0.013
0.203CysAsn: 0.203 ± 0.015
0.45CysPro: 0.45 ± 0.027
0.189CysGln: 0.189 ± 0.02
0.565CysArg: 0.565 ± 0.028
0.505CysSer: 0.505 ± 0.028
0.36CysThr: 0.36 ± 0.023
0.49CysVal: 0.49 ± 0.027
0.1CysTrp: 0.1 ± 0.012
0.145CysTyr: 0.145 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.469AspAla: 6.469 ± 0.102
0.551AspCys: 0.551 ± 0.03
3.295AspAsp: 3.295 ± 0.088
3.99AspGlu: 3.99 ± 0.096
2.229AspPhe: 2.229 ± 0.064
5.485AspGly: 5.485 ± 0.121
1.259AspHis: 1.259 ± 0.043
2.83AspIle: 2.83 ± 0.072
1.963AspLys: 1.963 ± 0.065
5.727AspLeu: 5.727 ± 0.104
1.213AspMet: 1.213 ± 0.046
1.355AspAsn: 1.355 ± 0.046
3.841AspPro: 3.841 ± 0.084
1.797AspGln: 1.797 ± 0.066
4.99AspArg: 4.99 ± 0.102
2.936AspSer: 2.936 ± 0.062
2.395AspThr: 2.395 ± 0.056
4.0AspVal: 4.0 ± 0.092
1.122AspTrp: 1.122 ± 0.045
1.553AspTyr: 1.553 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
8.178GluAla: 8.178 ± 0.147
0.405GluCys: 0.405 ± 0.029
2.913GluAsp: 2.913 ± 0.071
3.769GluGlu: 3.769 ± 0.1
1.767GluPhe: 1.767 ± 0.054
4.751GluGly: 4.751 ± 0.107
1.164GluHis: 1.164 ± 0.044
3.19GluIle: 3.19 ± 0.077
2.4GluLys: 2.4 ± 0.071
5.615GluLeu: 5.615 ± 0.118
1.523GluMet: 1.523 ± 0.05
1.501GluAsn: 1.501 ± 0.048
2.893GluPro: 2.893 ± 0.074
2.302GluGln: 2.302 ± 0.056
5.55GluArg: 5.55 ± 0.102
2.827GluSer: 2.827 ± 0.078
3.097GluThr: 3.097 ± 0.066
3.989GluVal: 3.989 ± 0.104
0.817GluTrp: 0.817 ± 0.04
1.011GluTyr: 1.011 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
4.769PheAla: 4.769 ± 0.086
0.311PheCys: 0.311 ± 0.021
2.697PheAsp: 2.697 ± 0.073
2.223PheGlu: 2.223 ± 0.066
1.321PhePhe: 1.321 ± 0.052
3.43PheGly: 3.43 ± 0.073
0.713PheHis: 0.713 ± 0.035
1.503PheIle: 1.503 ± 0.05
0.965PheLys: 0.965 ± 0.048
3.298PheLeu: 3.298 ± 0.077
0.645PheMet: 0.645 ± 0.035
0.988PheAsn: 0.988 ± 0.04
1.49PhePro: 1.49 ± 0.046
0.894PheGln: 0.894 ± 0.039
2.218PheArg: 2.218 ± 0.063
1.931PheSer: 1.931 ± 0.058
1.926PheThr: 1.926 ± 0.059
2.746PheVal: 2.746 ± 0.077
0.508PheTrp: 0.508 ± 0.031
0.882PheTyr: 0.882 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
9.505GlyAla: 9.505 ± 0.152
0.854GlyCys: 0.854 ± 0.04
4.942GlyAsp: 4.942 ± 0.091
5.284GlyGlu: 5.284 ± 0.109
3.481GlyPhe: 3.481 ± 0.085
8.121GlyGly: 8.121 ± 0.202
1.841GlyHis: 1.841 ± 0.051
4.623GlyIle: 4.623 ± 0.098
3.755GlyLys: 3.755 ± 0.09
8.387GlyLeu: 8.387 ± 0.12
2.192GlyMet: 2.192 ± 0.053
2.517GlyAsn: 2.517 ± 0.108
3.706GlyPro: 3.706 ± 0.075
3.056GlyGln: 3.056 ± 0.07
6.39GlyArg: 6.39 ± 0.107
5.094GlySer: 5.094 ± 0.105
4.716GlyThr: 4.716 ± 0.107
5.827GlyVal: 5.827 ± 0.093
1.578GlyTrp: 1.578 ± 0.055
2.246GlyTyr: 2.246 ± 0.071
0.0GlyXaa: 0.0 ± 0.0
His
2.012HisAla: 2.012 ± 0.062
0.263HisCys: 0.263 ± 0.02
1.192HisAsp: 1.192 ± 0.045
1.055HisGlu: 1.055 ± 0.046
0.822HisPhe: 0.822 ± 0.035
1.907HisGly: 1.907 ± 0.061
0.508HisHis: 0.508 ± 0.031
0.825HisIle: 0.825 ± 0.04
0.523HisLys: 0.523 ± 0.033
1.889HisLeu: 1.889 ± 0.061
0.414HisMet: 0.414 ± 0.028
0.42HisAsn: 0.42 ± 0.025
1.195HisPro: 1.195 ± 0.042
0.519HisGln: 0.519 ± 0.032
1.412HisArg: 1.412 ± 0.055
0.973HisSer: 0.973 ± 0.039
0.58HisThr: 0.58 ± 0.028
1.412HisVal: 1.412 ± 0.048
0.339HisTrp: 0.339 ± 0.024
0.55HisTyr: 0.55 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
7.818IleAla: 7.818 ± 0.109
0.371IleCys: 0.371 ± 0.027
3.99IleAsp: 3.99 ± 0.084
3.703IleGlu: 3.703 ± 0.084
1.59IlePhe: 1.59 ± 0.056
5.093IleGly: 5.093 ± 0.103
0.956IleHis: 0.956 ± 0.038
2.088IleIle: 2.088 ± 0.064
1.289IleLys: 1.289 ± 0.044
4.465IleLeu: 4.465 ± 0.09
0.83IleMet: 0.83 ± 0.033
1.352IleAsn: 1.352 ± 0.045
2.295IlePro: 2.295 ± 0.067
1.178IleGln: 1.178 ± 0.042
3.432IleArg: 3.432 ± 0.079
2.648IleSer: 2.648 ± 0.063
2.431IleThr: 2.431 ± 0.079
4.295IleVal: 4.295 ± 0.086
0.547IleTrp: 0.547 ± 0.03
1.036IleTyr: 1.036 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.64LysAla: 4.64 ± 0.098
0.202LysCys: 0.202 ± 0.019
1.861LysAsp: 1.861 ± 0.063
1.627LysGlu: 1.627 ± 0.051
0.959LysPhe: 0.959 ± 0.041
2.837LysGly: 2.837 ± 0.07
0.55LysHis: 0.55 ± 0.031
1.743LysIle: 1.743 ± 0.052
1.463LysLys: 1.463 ± 0.057
3.738LysLeu: 3.738 ± 0.091
0.842LysMet: 0.842 ± 0.036
0.837LysAsn: 0.837 ± 0.036
2.208LysPro: 2.208 ± 0.06
1.028LysGln: 1.028 ± 0.042
2.74LysArg: 2.74 ± 0.067
1.972LysSer: 1.972 ± 0.064
1.821LysThr: 1.821 ± 0.061
2.593LysVal: 2.593 ± 0.074
0.48LysTrp: 0.48 ± 0.024
0.67LysTyr: 0.67 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
13.986LeuAla: 13.986 ± 0.183
0.68LeuCys: 0.68 ± 0.031
6.28LeuAsp: 6.28 ± 0.098
5.456LeuGlu: 5.456 ± 0.107
3.69LeuPhe: 3.69 ± 0.09
8.554LeuGly: 8.554 ± 0.135
1.747LeuHis: 1.747 ± 0.048
4.882LeuIle: 4.882 ± 0.098
3.816LeuLys: 3.816 ± 0.083
9.697LeuLeu: 9.697 ± 0.159
2.034LeuMet: 2.034 ± 0.058
2.468LeuAsn: 2.468 ± 0.066
5.111LeuPro: 5.111 ± 0.099
2.494LeuGln: 2.494 ± 0.065
6.417LeuArg: 6.417 ± 0.116
5.959LeuSer: 5.959 ± 0.097
5.384LeuThr: 5.384 ± 0.096
7.457LeuVal: 7.457 ± 0.126
1.182LeuTrp: 1.182 ± 0.044
1.947LeuTyr: 1.947 ± 0.062
0.0LeuXaa: 0.0 ± 0.0
Met
3.148MetAla: 3.148 ± 0.073
0.146MetCys: 0.146 ± 0.015
1.119MetAsp: 1.119 ± 0.039
1.082MetGlu: 1.082 ± 0.042
0.707MetPhe: 0.707 ± 0.035
1.721MetGly: 1.721 ± 0.059
0.411MetHis: 0.411 ± 0.025
1.516MetIle: 1.516 ± 0.048
1.015MetLys: 1.015 ± 0.046
2.452MetLeu: 2.452 ± 0.067
0.665MetMet: 0.665 ± 0.038
0.722MetAsn: 0.722 ± 0.036
1.387MetPro: 1.387 ± 0.039
0.664MetGln: 0.664 ± 0.028
1.855MetArg: 1.855 ± 0.056
1.587MetSer: 1.587 ± 0.049
1.633MetThr: 1.633 ± 0.053
1.606MetVal: 1.606 ± 0.054
0.234MetTrp: 0.234 ± 0.021
0.237MetTyr: 0.237 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.308AsnAla: 3.308 ± 0.091
0.234AsnCys: 0.234 ± 0.019
1.464AsnAsp: 1.464 ± 0.053
1.293AsnGlu: 1.293 ± 0.043
0.91AsnPhe: 0.91 ± 0.041
2.569AsnGly: 2.569 ± 0.086
0.513AsnHis: 0.513 ± 0.03
1.369AsnIle: 1.369 ± 0.048
0.724AsnLys: 0.724 ± 0.037
2.477AsnLeu: 2.477 ± 0.065
0.611AsnMet: 0.611 ± 0.032
0.708AsnAsn: 0.708 ± 0.044
1.74AsnPro: 1.74 ± 0.056
0.785AsnGln: 0.785 ± 0.037
1.931AsnArg: 1.931 ± 0.054
1.333AsnSer: 1.333 ± 0.048
0.979AsnThr: 0.979 ± 0.046
1.989AsnVal: 1.989 ± 0.061
0.423AsnTrp: 0.423 ± 0.025
0.705AsnTyr: 0.705 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
6.375ProAla: 6.375 ± 0.11
0.303ProCys: 0.303 ± 0.02
3.505ProAsp: 3.505 ± 0.071
3.495ProGlu: 3.495 ± 0.059
1.824ProPhe: 1.824 ± 0.049
4.66ProGly: 4.66 ± 0.1
0.975ProHis: 0.975 ± 0.036
2.516ProIle: 2.516 ± 0.057
1.884ProLys: 1.884 ± 0.058
4.766ProLeu: 4.766 ± 0.085
1.175ProMet: 1.175 ± 0.042
1.421ProAsn: 1.421 ± 0.059
2.925ProPro: 2.925 ± 0.142
1.581ProGln: 1.581 ± 0.055
2.954ProArg: 2.954 ± 0.075
2.974ProSer: 2.974 ± 0.072
2.576ProThr: 2.576 ± 0.062
4.147ProVal: 4.147 ± 0.088
0.653ProTrp: 0.653 ± 0.03
1.092ProTyr: 1.092 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
3.864GlnAla: 3.864 ± 0.092
0.232GlnCys: 0.232 ± 0.018
1.285GlnAsp: 1.285 ± 0.045
1.312GlnGlu: 1.312 ± 0.048
1.128GlnPhe: 1.128 ± 0.044
2.38GlnGly: 2.38 ± 0.074
0.523GlnHis: 0.523 ± 0.028
1.479GlnIle: 1.479 ± 0.046
1.019GlnLys: 1.019 ± 0.041
3.198GlnLeu: 3.198 ± 0.08
0.844GlnMet: 0.844 ± 0.036
0.688GlnAsn: 0.688 ± 0.03
1.821GlnPro: 1.821 ± 0.06
1.062GlnGln: 1.062 ± 0.05
2.525GlnArg: 2.525 ± 0.068
1.877GlnSer: 1.877 ± 0.054
1.493GlnThr: 1.493 ± 0.042
2.269GlnVal: 2.269 ± 0.054
0.422GlnTrp: 0.422 ± 0.023
0.613GlnTyr: 0.613 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
8.159ArgAla: 8.159 ± 0.133
0.547ArgCys: 0.547 ± 0.032
4.189ArgAsp: 4.189 ± 0.082
4.483ArgGlu: 4.483 ± 0.088
3.061ArgPhe: 3.061 ± 0.072
5.134ArgGly: 5.134 ± 0.103
1.546ArgHis: 1.546 ± 0.052
4.175ArgIle: 4.175 ± 0.082
2.337ArgLys: 2.337 ± 0.06
8.495ArgLeu: 8.495 ± 0.142
2.049ArgMet: 2.049 ± 0.06
1.914ArgAsn: 1.914 ± 0.054
3.616ArgPro: 3.616 ± 0.083
2.591ArgGln: 2.591 ± 0.069
5.989ArgArg: 5.989 ± 0.115
4.206ArgSer: 4.206 ± 0.094
3.319ArgThr: 3.319 ± 0.066
4.677ArgVal: 4.677 ± 0.096
1.282ArgTrp: 1.282 ± 0.047
1.767ArgTyr: 1.767 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
6.942SerAla: 6.942 ± 0.102
0.389SerCys: 0.389 ± 0.025
3.27SerAsp: 3.27 ± 0.07
3.185SerGlu: 3.185 ± 0.068
2.1SerPhe: 2.1 ± 0.056
5.838SerGly: 5.838 ± 0.111
0.925SerHis: 0.925 ± 0.038
2.842SerIle: 2.842 ± 0.057
1.86SerLys: 1.86 ± 0.059
5.547SerLeu: 5.547 ± 0.103
1.261SerMet: 1.261 ± 0.044
1.504SerAsn: 1.504 ± 0.052
2.925SerPro: 2.925 ± 0.063
1.589SerGln: 1.589 ± 0.046
3.872SerArg: 3.872 ± 0.074
3.222SerSer: 3.222 ± 0.075
2.633SerThr: 2.633 ± 0.067
3.716SerVal: 3.716 ± 0.087
0.765SerTrp: 0.765 ± 0.036
1.201SerTyr: 1.201 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
6.009ThrAla: 6.009 ± 0.126
0.417ThrCys: 0.417 ± 0.025
2.668ThrAsp: 2.668 ± 0.065
2.569ThrGlu: 2.569 ± 0.057
1.864ThrPhe: 1.864 ± 0.061
5.083ThrGly: 5.083 ± 0.115
0.83ThrHis: 0.83 ± 0.036
2.96ThrIle: 2.96 ± 0.066
1.476ThrLys: 1.476 ± 0.044
5.217ThrLeu: 5.217 ± 0.087
1.225ThrMet: 1.225 ± 0.046
1.273ThrAsn: 1.273 ± 0.042
2.957ThrPro: 2.957 ± 0.068
1.321ThrGln: 1.321 ± 0.043
3.21ThrArg: 3.21 ± 0.075
2.76ThrSer: 2.76 ± 0.062
2.606ThrThr: 2.606 ± 0.088
4.069ThrVal: 4.069 ± 0.107
0.567ThrTrp: 0.567 ± 0.036
1.096ThrTyr: 1.096 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
9.657ValAla: 9.657 ± 0.131
0.488ValCys: 0.488 ± 0.029
4.409ValAsp: 4.409 ± 0.092
4.859ValGlu: 4.859 ± 0.092
1.98ValPhe: 1.98 ± 0.057
5.862ValGly: 5.862 ± 0.103
1.324ValHis: 1.324 ± 0.049
3.883ValIle: 3.883 ± 0.08
2.354ValLys: 2.354 ± 0.07
6.22ValLeu: 6.22 ± 0.109
1.621ValMet: 1.621 ± 0.051
1.924ValAsn: 1.924 ± 0.061
3.935ValPro: 3.935 ± 0.075
1.867ValGln: 1.867 ± 0.058
5.328ValArg: 5.328 ± 0.097
3.933ValSer: 3.933 ± 0.075
4.366ValThr: 4.366 ± 0.084
5.327ValVal: 5.327 ± 0.1
0.876ValTrp: 0.876 ± 0.045
1.192ValTyr: 1.192 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
1.459TrpAla: 1.459 ± 0.051
0.117TrpCys: 0.117 ± 0.013
0.693TrpAsp: 0.693 ± 0.031
0.576TrpGlu: 0.576 ± 0.031
0.47TrpPhe: 0.47 ± 0.028
0.971TrpGly: 0.971 ± 0.046
0.323TrpHis: 0.323 ± 0.02
0.764TrpIle: 0.764 ± 0.038
0.52TrpLys: 0.52 ± 0.029
1.772TrpLeu: 1.772 ± 0.059
0.34TrpMet: 0.34 ± 0.021
0.413TrpAsn: 0.413 ± 0.029
0.719TrpPro: 0.719 ± 0.032
0.56TrpGln: 0.56 ± 0.029
1.256TrpArg: 1.256 ± 0.048
0.931TrpSer: 0.931 ± 0.034
0.814TrpThr: 0.814 ± 0.033
0.871TrpVal: 0.871 ± 0.038
0.268TrpTrp: 0.268 ± 0.021
0.276TrpTyr: 0.276 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.462TyrAla: 2.462 ± 0.065
0.208TyrCys: 0.208 ± 0.017
1.524TyrAsp: 1.524 ± 0.058
1.115TyrGlu: 1.115 ± 0.043
0.862TyrPhe: 0.862 ± 0.038
2.097TyrGly: 2.097 ± 0.073
0.397TyrHis: 0.397 ± 0.023
0.737TyrIle: 0.737 ± 0.033
0.607TyrLys: 0.607 ± 0.034
2.165TyrLeu: 2.165 ± 0.062
0.368TyrMet: 0.368 ± 0.025
0.577TyrAsn: 0.577 ± 0.035
1.038TyrPro: 1.038 ± 0.044
0.657TyrGln: 0.657 ± 0.034
1.978TyrArg: 1.978 ± 0.062
1.245TyrSer: 1.245 ± 0.047
0.817TyrThr: 0.817 ± 0.033
1.56TyrVal: 1.56 ± 0.056
0.382TyrTrp: 0.382 ± 0.022
0.571TyrTyr: 0.571 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2226 proteins (649559 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski