Angomonas deanei
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G2CJY9|A0A7G2CJY9_9TRYP Uncharacterized protein OS=Angomonas deanei OX=59799 GN=ADEAN_000769300 PE=4 SV=1
MM1 pKa = 7.08 FKK3 pKa = 10.78 VMRR6 pKa = 11.84 TLLLSLLLLATTLHH20 pKa = 6.6 ASSSDD25 pKa = 3.36 SGSNSTDD32 pKa = 2.96 SGSNSTDD39 pKa = 2.96 SGSNSTDD46 pKa = 2.96 SGSNSTDD53 pKa = 2.98 SSDD56 pKa = 4.92 SYY58 pKa = 11.45 LDD60 pKa = 3.51 HH61 pKa = 7.16 NKK63 pKa = 9.03 TDD65 pKa = 3.67 SGSGSHH71 pKa = 6.84 HH72 pKa = 6.84 SDD74 pKa = 2.97 SGNSPDD80 pKa = 4.13 SNHH83 pKa = 6.41 SGSLPTDD90 pKa = 3.55 SSADD94 pKa = 3.54 DD95 pKa = 3.83 SALSDD100 pKa = 4.48 SDD102 pKa = 5.76 DD103 pKa = 3.72 GVCNVTHH110 pKa = 6.87 CKK112 pKa = 10.04 EE113 pKa = 3.88 CAKK116 pKa = 10.74 RR117 pKa = 11.84 NVCLQCEE124 pKa = 4.28 DD125 pKa = 4.65 HH126 pKa = 6.9 YY127 pKa = 11.63 VLPPSSSTRR136 pKa = 11.84 CVPVMDD142 pKa = 4.31 GCLVYY147 pKa = 9.96 TVNANVCQQCKK158 pKa = 9.86 EE159 pKa = 4.2 GYY161 pKa = 7.53 TLEE164 pKa = 4.34 TGGTCTGTKK173 pKa = 9.92 KK174 pKa = 10.74 KK175 pKa = 10.57 EE176 pKa = 3.91 GTQWWVWLIVALAIVVVIVIIIIIICCCCCKK207 pKa = 9.98 KK208 pKa = 10.86 DD209 pKa = 3.36 KK210 pKa = 7.8 TTPTRR215 pKa = 11.84 GTSTTTSRR223 pKa = 11.84 STSTNSSFSSKK234 pKa = 9.16 TATGEE239 pKa = 4.05 DD240 pKa = 4.36 DD241 pKa = 4.55 SDD243 pKa = 5.37 SSDD246 pKa = 5.77 DD247 pKa = 6.14 DD248 pKa = 6.46 DD249 pKa = 7.69 DD250 pKa = 7.65 DD251 pKa = 7.64 DD252 pKa = 7.67 DD253 pKa = 7.6 DD254 pKa = 7.56 DD255 pKa = 7.19 DD256 pKa = 7.37 DD257 pKa = 5.61 EE258 pKa = 7.83 DD259 pKa = 6.39 SDD261 pKa = 6.34 DD262 pKa = 4.81 DD263 pKa = 6.15 DD264 pKa = 5.15 EE265 pKa = 7.02 DD266 pKa = 4.48 SSEE269 pKa = 4.27 DD270 pKa = 3.47 SSEE273 pKa = 3.98 EE274 pKa = 3.84 SSGDD278 pKa = 3.65 EE279 pKa = 4.5 EE280 pKa = 4.72 EE281 pKa = 5.07 DD282 pKa = 4.3 SSDD285 pKa = 3.48 EE286 pKa = 4.1 SDD288 pKa = 3.91 AEE290 pKa = 4.55 GSAGTAEE297 pKa = 4.13 ATVV300 pKa = 3.27
Molecular weight: 31.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.923
Patrickios 1.354
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A7G2CE56|A0A7G2CE56_9TRYP Uncharacterized protein OS=Angomonas deanei OX=59799 GN=ADEAN_000471900 PE=4 SV=1
MM1 pKa = 7.31 VIALATRR8 pKa = 11.84 QTAMRR13 pKa = 11.84 IVPATQQATTAAAPAVQWVTRR34 pKa = 11.84 RR35 pKa = 11.84 IALAVHH41 pKa = 6.81 RR42 pKa = 11.84 PTLKK46 pKa = 10.31 KK47 pKa = 10.74 VPAAQRR53 pKa = 11.84 ATLRR57 pKa = 11.84 RR58 pKa = 11.84 ARR60 pKa = 11.84 QATTMIARR68 pKa = 11.84 AVRR71 pKa = 11.84 WKK73 pKa = 8.97 ITATAPVMRR82 pKa = 11.84 PSPPATALVPRR93 pKa = 11.84 KK94 pKa = 8.64 ATLARR99 pKa = 11.84 VTKK102 pKa = 10.43 AALIVAKK109 pKa = 10.15 AARR112 pKa = 11.84 RR113 pKa = 11.84 LARR116 pKa = 11.84 TIALMIRR123 pKa = 11.84 QATPTKK129 pKa = 10.51 VRR131 pKa = 11.84 AALQAKK137 pKa = 8.01 LTAAPVAQQATMTKK151 pKa = 8.13 TPVPHH156 pKa = 7.21 RR157 pKa = 11.84 VALWLATALMTLPATPKK174 pKa = 9.97 KK175 pKa = 10.18 VRR177 pKa = 11.84 AVRR180 pKa = 11.84 WKK182 pKa = 10.26 IKK184 pKa = 9.49 ATTPVTRR191 pKa = 11.84 PSPPTTALVMQQSFLTRR208 pKa = 11.84 VRR210 pKa = 11.84 AVRR213 pKa = 11.84 RR214 pKa = 11.84 ATLPTAPVMQQSTPKK229 pKa = 10.06 KK230 pKa = 8.83 VRR232 pKa = 11.84 AAQWVNLKK240 pKa = 9.94 RR241 pKa = 11.84 VPAMPRR247 pKa = 11.84 ATMRR251 pKa = 11.84 KK252 pKa = 7.91 APAVPRR258 pKa = 11.84 VITMRR263 pKa = 11.84 GPAMQRR269 pKa = 11.84 ATPRR273 pKa = 11.84 LAAPPVRR280 pKa = 11.84 PRR282 pKa = 11.84 TTRR285 pKa = 11.84 RR286 pKa = 11.84 AALPPQRR293 pKa = 11.84 HH294 pKa = 4.84 NPKK297 pKa = 10.23 RR298 pKa = 11.84 MRR300 pKa = 11.84 NDD302 pKa = 3.15 LVSKK306 pKa = 10.51 RR307 pKa = 11.84 KK308 pKa = 9.48 ADD310 pKa = 3.91 RR311 pKa = 11.84 PIAPLRR317 pKa = 11.84 PLTEE321 pKa = 3.86 QSFSSARR328 pKa = 11.84 CQAALPSVTGALGKK342 pKa = 10.34
Molecular weight: 37.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.51
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.588
Grimsley 12.954
Solomon 13.422
Lehninger 13.32
Nozaki 12.925
DTASelect 12.91
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.296
IPC_peptide 13.422
IPC2_peptide 12.413
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10115
0
10115
4218491
51
4916
417.1
46.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.764 ± 0.04
1.917 ± 0.025
5.31 ± 0.025
7.451 ± 0.043
3.791 ± 0.018
5.788 ± 0.026
2.373 ± 0.013
4.086 ± 0.017
5.563 ± 0.026
9.472 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.012
4.356 ± 0.027
5.033 ± 0.023
4.001 ± 0.026
6.005 ± 0.025
7.811 ± 0.029
6.05 ± 0.028
7.049 ± 0.022
0.995 ± 0.007
2.987 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here