Arthrobacter phage Abba
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8R2B7|A0A6G8R2B7_9CAUD Portal protein OS=Arthrobacter phage Abba OX=2713256 GN=3 PE=4 SV=1
MM1 pKa = 6.83 TTTFEE6 pKa = 4.11 QARR9 pKa = 11.84 KK10 pKa = 9.04 IASEE14 pKa = 4.17 LNAPAWRR21 pKa = 11.84 DD22 pKa = 3.11 LGNRR26 pKa = 11.84 GEE28 pKa = 4.87 YY29 pKa = 7.43 MTADD33 pKa = 3.58 YY34 pKa = 10.49 GYY36 pKa = 9.8 EE37 pKa = 4.01 NADD40 pKa = 2.55 AWLIVEE46 pKa = 4.91 GARR49 pKa = 11.84 EE50 pKa = 4.1 YY51 pKa = 10.78 IEE53 pKa = 5.72 GGDD56 pKa = 3.73 DD57 pKa = 3.52 SFVILDD63 pKa = 3.66 QPLTLVMKK71 pKa = 9.72 ATGDD75 pKa = 3.91 VVSVQYY81 pKa = 11.22 LDD83 pKa = 4.27 DD84 pKa = 5.24 PEE86 pKa = 7.03 LIDD89 pKa = 4.96 GMTPVGNAPTYY100 pKa = 9.53 EE101 pKa = 3.94
Molecular weight: 11.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.004
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.579
ProMoST 3.897
Dawson 3.757
Bjellqvist 3.973
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.897
Patrickios 2.931
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A6G8R2E2|A0A6G8R2E2_9CAUD Uncharacterized protein OS=Arthrobacter phage Abba OX=2713256 GN=17 PE=4 SV=1
MM1 pKa = 6.79 TTTHH5 pKa = 5.85 STLAALIVEE14 pKa = 4.52 EE15 pKa = 4.27 NDD17 pKa = 3.45 RR18 pKa = 11.84 ADD20 pKa = 3.68 RR21 pKa = 11.84 AQADD25 pKa = 4.09 RR26 pKa = 11.84 QRR28 pKa = 11.84 GRR30 pKa = 11.84 NEE32 pKa = 3.84 PEE34 pKa = 3.86 HH35 pKa = 6.73 LGQARR40 pKa = 11.84 LLAATRR46 pKa = 11.84 EE47 pKa = 3.99 IAEE50 pKa = 4.13 YY51 pKa = 10.23 NARR54 pKa = 11.84 NPHH57 pKa = 5.82 PQTIKK62 pKa = 10.29 RR63 pKa = 11.84 RR64 pKa = 11.84 ARR66 pKa = 11.84 KK67 pKa = 9.24 AKK69 pKa = 9.54 QQ70 pKa = 2.79
Molecular weight: 8.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.56
IPC_protein 10.672
Toseland 10.906
ProMoST 10.921
Dawson 10.95
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 10.95
Grimsley 10.994
Solomon 11.228
Lehninger 11.169
Nozaki 10.891
DTASelect 10.76
Thurlkill 10.891
EMBOSS 11.345
Sillero 10.906
Patrickios 10.76
IPC_peptide 11.242
IPC2_peptide 9.853
IPC2.peptide.svr19 8.923
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
14884
41
1067
209.6
22.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.048 ± 0.515
0.504 ± 0.093
6.564 ± 0.216
5.724 ± 0.353
3.225 ± 0.206
8.492 ± 0.448
1.747 ± 0.17
4.3 ± 0.197
4.26 ± 0.219
8.318 ± 0.243
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.123 ± 0.119
3.023 ± 0.169
5.523 ± 0.351
3.084 ± 0.183
6.316 ± 0.378
5.55 ± 0.282
6.739 ± 0.325
7.646 ± 0.346
1.586 ± 0.162
2.231 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here