Aeropyrum pernix spindle-shaped virus 1

Taxonomy: Viruses; unclassified archaeal viruses

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3CAY6|G3CAY6_9VIRU Uncharacterized protein OS=Aeropyrum pernix spindle-shaped virus 1 OX=1032473 PE=4 SV=1
MM1 pKa = 7.3QGFTAYY7 pKa = 10.44LVGFAIVAGFAVALGFIFGIFNQIVAQLNTDD38 pKa = 3.81PNNPMISQEE47 pKa = 4.08FVDD50 pKa = 6.1AINLAQSLGGTGLTLAIAALIVGLAMLLIAVIRR83 pKa = 11.84SYY85 pKa = 11.52SGVV88 pKa = 3.23

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3CAY0|G3CAY0_9VIRU Uncharacterized protein OS=Aeropyrum pernix spindle-shaped virus 1 OX=1032473 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 5.04GGGSAGSAPSSPGSSRR18 pKa = 11.84PPDD21 pKa = 3.34YY22 pKa = 11.03SLYY25 pKa = 10.34EE26 pKa = 4.16SQPRR30 pKa = 11.84PAPASWSPLGVLASALDD47 pKa = 3.6APYY50 pKa = 11.09SFVQEE55 pKa = 4.27RR56 pKa = 11.84GWGVVEE62 pKa = 4.06RR63 pKa = 11.84LRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84ALRR70 pKa = 11.84EE71 pKa = 3.79KK72 pKa = 11.22AEE74 pKa = 3.9GDD76 pKa = 3.74YY77 pKa = 11.74AEE79 pKa = 4.61GFLWALGASAASFGVGVAAGVTGLFSPRR107 pKa = 11.84AWRR110 pKa = 11.84EE111 pKa = 3.48AFHH114 pKa = 8.07AFMHH118 pKa = 6.42PGEE121 pKa = 4.35TVRR124 pKa = 11.84AVASDD129 pKa = 3.94PLSWPYY135 pKa = 10.76LAGSVVGPAKK145 pKa = 10.52LGRR148 pKa = 11.84AVGRR152 pKa = 11.84RR153 pKa = 11.84LGRR156 pKa = 11.84VRR158 pKa = 11.84EE159 pKa = 4.06PDD161 pKa = 3.27LASTSEE167 pKa = 4.04GSLTLVAAKK176 pKa = 10.28GEE178 pKa = 4.14RR179 pKa = 11.84LRR181 pKa = 11.84WAARR185 pKa = 11.84LEE187 pKa = 4.08ARR189 pKa = 11.84GPTVGVKK196 pKa = 9.63GVKK199 pKa = 9.24PRR201 pKa = 11.84PPPDD205 pKa = 3.1RR206 pKa = 11.84LVTLEE211 pKa = 4.04TPRR214 pKa = 11.84GRR216 pKa = 11.84VEE218 pKa = 3.91LLEE221 pKa = 4.09SRR223 pKa = 11.84RR224 pKa = 11.84GSTVRR229 pKa = 11.84RR230 pKa = 11.84VYY232 pKa = 9.91RR233 pKa = 11.84AEE235 pKa = 3.71YY236 pKa = 10.09RR237 pKa = 11.84DD238 pKa = 3.59VGKK241 pKa = 10.75KK242 pKa = 10.29LLGVEE247 pKa = 4.27EE248 pKa = 4.51WSVEE252 pKa = 3.28GWLRR256 pKa = 11.84PRR258 pKa = 11.84YY259 pKa = 9.28RR260 pKa = 11.84YY261 pKa = 9.79RR262 pKa = 11.84GLLVDD267 pKa = 4.64PEE269 pKa = 4.65TPGAAVLLEE278 pKa = 3.79ARR280 pKa = 11.84RR281 pKa = 11.84PLLRR285 pKa = 11.84GVDD288 pKa = 4.22LPPPRR293 pKa = 11.84LWVEE297 pKa = 3.72PRR299 pKa = 11.84LAAPAGAVGLILGLGAAGTGARR321 pKa = 11.84GGLEE325 pKa = 3.8GGAAGRR331 pKa = 11.84PAEE334 pKa = 4.33AGARR338 pKa = 11.84GLVSGSSLAGGEE350 pKa = 4.2AAASTLAGASAASSPAEE367 pKa = 3.96PSAASAGRR375 pKa = 11.84GGPEE379 pKa = 3.92APPLLGSGSRR389 pKa = 11.84GRR391 pKa = 11.84RR392 pKa = 11.84GRR394 pKa = 11.84RR395 pKa = 11.84GKK397 pKa = 10.02LAYY400 pKa = 10.23SEE402 pKa = 4.01ILYY405 pKa = 10.02PGGVLLEE412 pKa = 4.25LLL414 pKa = 4.31

Molecular weight: 43.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11486

38

929

216.7

24.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.324 ± 0.421

0.609 ± 0.095

4.788 ± 0.239

7.191 ± 0.309

3.213 ± 0.209

8.375 ± 0.518

1.41 ± 0.134

4.78 ± 0.495

4.083 ± 0.428

11.675 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.147

2.046 ± 0.196

4.536 ± 0.293

2.168 ± 0.22

8.593 ± 0.582

6.26 ± 0.253

4.066 ± 0.286

8.523 ± 0.358

1.898 ± 0.214

4.423 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski