Citrus leprosis virus C2
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1JAW3|M1JAW3_9VIRU Helicase OS=Citrus leprosis virus C2 OX=2052685 PE=4 SV=1
MM1 pKa = 8.06 DD2 pKa = 5.45 FYY4 pKa = 11.13 TVYY7 pKa = 10.65 TFLNLTLGLLTYY19 pKa = 10.53 NYY21 pKa = 10.39 DD22 pKa = 3.47 SLGTFGPTPRR32 pKa = 11.84 CTSKK36 pKa = 10.7 RR37 pKa = 11.84 CSLLPAEE44 pKa = 5.59 CSTEE48 pKa = 3.82 HH49 pKa = 5.69 TVNKK53 pKa = 9.67 VVEE56 pKa = 4.26 RR57 pKa = 11.84 HH58 pKa = 5.0 VFTANDD64 pKa = 4.09 HH65 pKa = 5.74 YY66 pKa = 10.79 CISLGYY72 pKa = 9.53 RR73 pKa = 11.84 KK74 pKa = 10.18 NISFSVSTLFIGDD87 pKa = 3.48 YY88 pKa = 10.48 DD89 pKa = 4.04 VYY91 pKa = 11.44 GGFEE95 pKa = 4.45 CDD97 pKa = 2.88 RR98 pKa = 11.84 TALSMGIVSNRR109 pKa = 11.84 LGFYY113 pKa = 10.5 EE114 pKa = 5.77 DD115 pKa = 5.2 ALFDD119 pKa = 4.01 CKK121 pKa = 10.89 NYY123 pKa = 10.05 NLSYY127 pKa = 10.76 GNYY130 pKa = 9.39 RR131 pKa = 11.84 FEE133 pKa = 4.3 FCLNSSVYY141 pKa = 10.92 DD142 pKa = 3.53 SVLTDD147 pKa = 3.63 KK148 pKa = 10.98 FYY150 pKa = 10.91 TPQIEE155 pKa = 3.93 AAAPINTRR163 pKa = 11.84 GVEE166 pKa = 4.08 VFDD169 pKa = 4.95 DD170 pKa = 4.39 FLGFTYY176 pKa = 10.71 YY177 pKa = 9.17 RR178 pKa = 11.84 TSCDD182 pKa = 3.56 SFCSEE187 pKa = 4.97 SKK189 pKa = 10.65 FSPDD193 pKa = 2.99 FDD195 pKa = 3.94 FGNYY199 pKa = 7.4 VFYY202 pKa = 10.68 FPLCDD207 pKa = 3.38 NHH209 pKa = 7.87 IPLCYY214 pKa = 10.3 DD215 pKa = 3.39 GAEE218 pKa = 4.22 DD219 pKa = 3.76 SCPRR223 pKa = 11.84 GYY225 pKa = 10.78 SLQTIFVSSLVEE237 pKa = 4.0 EE238 pKa = 4.49 EE239 pKa = 4.37 TEE241 pKa = 3.74 KK242 pKa = 10.66 LARR245 pKa = 11.84 VICVSDD251 pKa = 3.89 NEE253 pKa = 4.46 PGLTFPAWFRR263 pKa = 11.84 DD264 pKa = 3.74 NPSTYY269 pKa = 10.96 DD270 pKa = 3.82 DD271 pKa = 4.25 LVGNYY276 pKa = 7.67 TINVEE281 pKa = 4.59 SKK283 pKa = 10.65 CGVAMSKK290 pKa = 10.58 KK291 pKa = 9.43 MRR293 pKa = 11.84 FSRR296 pKa = 11.84 YY297 pKa = 8.96 GVTGTYY303 pKa = 9.32 IDD305 pKa = 5.17 DD306 pKa = 4.71 IIVVTIDD313 pKa = 3.29 EE314 pKa = 4.51 VHH316 pKa = 6.32 FLTSKK321 pKa = 10.09 FCRR324 pKa = 11.84 DD325 pKa = 3.56 YY326 pKa = 11.37 ILFLGYY332 pKa = 9.85 RR333 pKa = 11.84 DD334 pKa = 4.94 GYY336 pKa = 11.62 DD337 pKa = 3.18 NDD339 pKa = 4.58 FSMKK343 pKa = 10.04 TYY345 pKa = 9.04 FQKK348 pKa = 10.87 RR349 pKa = 11.84 LNCNSDD355 pKa = 3.39 GCWYY359 pKa = 10.75 SGVDD363 pKa = 3.34 LSRR366 pKa = 11.84 ILSHH370 pKa = 7.07 CEE372 pKa = 3.12 LSLVVEE378 pKa = 4.46 KK379 pKa = 11.08 KK380 pKa = 9.64 EE381 pKa = 3.93 ALVSTFTLFNKK392 pKa = 9.33 TFGGKK397 pKa = 9.54 VGFIPVGLSDD407 pKa = 4.45 SVLFGYY413 pKa = 10.51 NVFLFRR419 pKa = 11.84 GFYY422 pKa = 9.92 SYY424 pKa = 11.33 SSSSVTLKK432 pKa = 8.39 STKK435 pKa = 10.09 YY436 pKa = 10.05 FLVKK440 pKa = 10.58 AEE442 pKa = 4.49 AEE444 pKa = 4.2 WYY446 pKa = 7.22 MKK448 pKa = 11.21 LMMFFADD455 pKa = 5.39 DD456 pKa = 3.55 VLKK459 pKa = 11.25 VCFEE463 pKa = 4.73 TIFSVLLGALSSCLSFIFNIGGCCFRR489 pKa = 11.84 LVFLCVMDD497 pKa = 4.69 SIIILLCLLPCYY509 pKa = 9.95 CHH511 pKa = 7.28 LGFILCSVSNLIIKK525 pKa = 10.47 LFMKK529 pKa = 10.24 NNCCFGISDD538 pKa = 4.25 AVASSFF544 pKa = 4.31
Molecular weight: 61.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.962
IPC2_protein 4.914
IPC_protein 4.876
Toseland 4.749
ProMoST 5.016
Dawson 4.863
Bjellqvist 5.003
Wikipedia 4.762
Rodwell 4.749
Grimsley 4.66
Solomon 4.863
Lehninger 4.825
Nozaki 4.978
DTASelect 5.181
Thurlkill 4.762
EMBOSS 4.787
Sillero 5.029
Patrickios 0.439
IPC_peptide 4.863
IPC2_peptide 5.029
IPC2.peptide.svr19 4.875
Protein with the highest isoelectric point:
>tr|M1J7K9|M1J7K9_9VIRU p61 OS=Citrus leprosis virus C2 OX=2052685 PE=4 SV=1
MM1 pKa = 7.77 DD2 pKa = 3.27 QRR4 pKa = 11.84 FLRR7 pKa = 11.84 GASVLNLSNKK17 pKa = 9.48 RR18 pKa = 11.84 EE19 pKa = 4.01 KK20 pKa = 10.98 LKK22 pKa = 10.42 TNASVSLLSDD32 pKa = 3.53 IQQILLRR39 pKa = 11.84 YY40 pKa = 7.55 IQKK43 pKa = 10.39 PYY45 pKa = 10.02 VLLMYY50 pKa = 10.67 ACVLVLFAMHH60 pKa = 6.85 IDD62 pKa = 3.84 AGTHH66 pKa = 7.5 DD67 pKa = 4.75 ILDD70 pKa = 4.44 DD71 pKa = 4.72 LAQQFPNNPVIEE83 pKa = 4.18 WARR86 pKa = 11.84 NNFFRR91 pKa = 11.84 LCGALVFIPVITDD104 pKa = 2.94 ARR106 pKa = 11.84 KK107 pKa = 8.45 EE108 pKa = 3.91 HH109 pKa = 5.76 QLYY112 pKa = 10.33 FGMVIALFLLGFPQRR127 pKa = 11.84 SIFEE131 pKa = 4.19 YY132 pKa = 10.2 FVYY135 pKa = 10.57 SLSLHH140 pKa = 6.29 VYY142 pKa = 9.79 AKK144 pKa = 10.14 SKK146 pKa = 11.06 HH147 pKa = 5.14 PFTRR151 pKa = 11.84 IFILVITVVSCVLFGVFTNDD171 pKa = 3.36 QLRR174 pKa = 11.84 KK175 pKa = 9.91 LYY177 pKa = 10.47 QEE179 pKa = 4.18 LPKK182 pKa = 10.78 VPTHH186 pKa = 6.25 PVNKK190 pKa = 9.71 VEE192 pKa = 4.09 RR193 pKa = 11.84 VVNRR197 pKa = 11.84 VGGQQSFSGG206 pKa = 3.64
Molecular weight: 23.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.883
IPC2_protein 8.99
IPC_protein 8.975
Toseland 9.443
ProMoST 9.311
Dawson 9.765
Bjellqvist 9.545
Wikipedia 9.97
Rodwell 9.955
Grimsley 9.853
Solomon 9.809
Lehninger 9.765
Nozaki 9.589
DTASelect 9.487
Thurlkill 9.589
EMBOSS 9.882
Sillero 9.721
Patrickios 4.8
IPC_peptide 9.809
IPC2_peptide 8.346
IPC2.peptide.svr19 7.882
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4007
67
2504
572.4
64.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.091 ± 0.488
2.945 ± 0.538
6.613 ± 0.597
4.842 ± 0.448
6.289 ± 0.819
5.141 ± 0.5
2.595 ± 0.311
6.114 ± 0.519
5.54 ± 0.755
9.733 ± 0.402
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.997 ± 0.286
4.817 ± 0.509
3.893 ± 1.183
2.62 ± 0.627
5.49 ± 0.535
8.56 ± 0.567
5.091 ± 0.42
8.111 ± 0.291
0.574 ± 0.092
3.943 ± 0.486
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here