Lysinibacillus antri
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3826 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432LD64|A0A432LD64_9BACI Uncharacterized protein OS=Lysinibacillus antri OX=2498145 GN=EK386_08520 PE=4 SV=1
MM1 pKa = 7.49 AAEE4 pKa = 3.99 QRR6 pKa = 11.84 LSQVLTKK13 pKa = 10.74 KK14 pKa = 10.33 LGYY17 pKa = 9.09 YY18 pKa = 9.95 VPISEE23 pKa = 5.11 IGYY26 pKa = 5.95 EE27 pKa = 4.51 TIWFMKK33 pKa = 10.72 DD34 pKa = 2.81 EE35 pKa = 4.56 VDD37 pKa = 3.97 NKK39 pKa = 10.62 LIPPQIIEE47 pKa = 4.78 HH48 pKa = 6.78 LAQPIIANSASFTDD62 pKa = 3.5 ADD64 pKa = 3.61 GNYY67 pKa = 9.08 YY68 pKa = 10.42 LVIIIEE74 pKa = 4.18 IGLPEE79 pKa = 4.97 PYY81 pKa = 10.05 EE82 pKa = 3.88 VWLVNDD88 pKa = 4.9 EE89 pKa = 4.21 IVSDD93 pKa = 3.79 FLGDD97 pKa = 3.23 VDD99 pKa = 4.37
Molecular weight: 11.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.694
ProMoST 3.948
Dawson 3.859
Bjellqvist 4.075
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.605
Solomon 3.834
Lehninger 3.795
Nozaki 3.986
DTASelect 4.151
Thurlkill 3.745
EMBOSS 3.783
Sillero 3.999
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|A0A432L934|A0A432L934_9BACI Uncharacterized protein OS=Lysinibacillus antri OX=2498145 GN=EK386_14720 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.54 QPKK8 pKa = 9.44 KK9 pKa = 7.96 RR10 pKa = 11.84 KK11 pKa = 8.69 HH12 pKa = 5.94 SKK14 pKa = 8.54 VHH16 pKa = 5.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.91 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3826
0
3826
1143930
33
2801
299.0
33.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.882 ± 0.041
0.695 ± 0.011
4.981 ± 0.03
7.629 ± 0.045
4.623 ± 0.032
6.464 ± 0.04
2.033 ± 0.021
8.355 ± 0.042
6.812 ± 0.033
9.701 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.657 ± 0.024
4.854 ± 0.033
3.411 ± 0.025
3.915 ± 0.032
3.785 ± 0.032
6.002 ± 0.03
5.674 ± 0.035
7.065 ± 0.034
0.93 ± 0.015
3.533 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here