Tuber melanosporum (strain Mel28) (Perigord black truffle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Pezizomycetes; Pezizales; Tuberaceae; Tuber; Tuber melanosporum

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7494 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5GCY9|D5GCY9_TUBMM Fe2OG dioxygenase domain-containing protein OS=Tuber melanosporum (strain Mel28) OX=656061 GN=GSTUM_00000881001 PE=3 SV=1
MM1 pKa = 8.11DD2 pKa = 5.19GGIGTSSMGYY12 pKa = 9.75YY13 pKa = 9.14MAGLEE18 pKa = 4.25VFTYY22 pKa = 10.5QSVEE26 pKa = 4.23EE27 pKa = 4.74IRR29 pKa = 11.84VGWDD33 pKa = 2.88WVIVEE38 pKa = 4.08MLEE41 pKa = 4.38VGGEE45 pKa = 4.04CSRR48 pKa = 11.84VEE50 pKa = 4.23DD51 pKa = 3.57VVEE54 pKa = 3.97AVLAIWKK61 pKa = 9.67QNSTLGLGQRR71 pKa = 11.84GWCDD75 pKa = 3.09IILL78 pKa = 4.04

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5GPT5|D5GPT5_TUBMM Uncharacterized protein OS=Tuber melanosporum (strain Mel28) OX=656061 GN=GSTUM_00012024001 PE=4 SV=1
MM1 pKa = 7.3FRR3 pKa = 11.84RR4 pKa = 11.84PLTLLHH10 pKa = 7.01RR11 pKa = 11.84LPQRR15 pKa = 11.84LPRR18 pKa = 11.84IATPPLTRR26 pKa = 11.84TIITKK31 pKa = 7.05TTPLRR36 pKa = 11.84PSTISFTPAGLATPVPTSTGVGLAVTAPLMLKK68 pKa = 10.14ISSNPGLVGLQVRR81 pKa = 11.84CGPRR85 pKa = 11.84DD86 pKa = 3.45TYY88 pKa = 11.42NPSHH92 pKa = 7.01RR93 pKa = 11.84VRR95 pKa = 11.84KK96 pKa = 9.46RR97 pKa = 11.84RR98 pKa = 11.84LGFLARR104 pKa = 11.84KK105 pKa = 6.53RR106 pKa = 11.84TPGGRR111 pKa = 11.84GVLRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84LKK120 pKa = 10.09GRR122 pKa = 11.84KK123 pKa = 8.99SLTHH127 pKa = 6.24

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7494

0

7494

3279832

10

9545

437.7

48.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.886 ± 0.031

1.212 ± 0.01

5.241 ± 0.024

6.573 ± 0.034

3.639 ± 0.02

7.488 ± 0.033

2.301 ± 0.015

4.943 ± 0.024

5.249 ± 0.029

8.856 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.137 ± 0.012

3.592 ± 0.018

6.265 ± 0.039

3.539 ± 0.023

6.359 ± 0.031

8.263 ± 0.042

5.851 ± 0.03

6.214 ± 0.027

1.294 ± 0.01

2.683 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski