Amino acid dipepetide frequency for Tuber melanosporum (strain Mel28) (Perigord black truffle)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.646AlaAla: 7.646 ± 0.079	0.907AlaCys: 0.907 ± 0.016	3.508AlaAsp: 3.508 ± 0.032	5.031AlaGlu: 5.031 ± 0.053	2.768AlaPhe: 2.768 ± 0.035	5.925AlaGly: 5.925 ± 0.05	1.63AlaHis: 1.63 ± 0.027	4.078AlaIle: 4.078 ± 0.036	4.084AlaLys: 4.084 ± 0.042	7.261AlaLeu: 7.261 ± 0.055	1.734AlaMet: 1.734 ± 0.024	2.647AlaAsn: 2.647 ± 0.03	4.716AlaPro: 4.716 ± 0.057	2.79AlaGln: 2.79 ± 0.038	4.79AlaArg: 4.79 ± 0.047	6.501AlaSer: 6.501 ± 0.056	4.859AlaThr: 4.859 ± 0.047	5.151AlaVal: 5.151 ± 0.04	0.931AlaTrp: 0.931 ± 0.021	1.897AlaTyr: 1.897 ± 0.027	0.002AlaXaa: 0.002 ± 0.001
Cys	0.889CysAla: 0.889 ± 0.015	0.257CysCys: 0.257 ± 0.009	0.614CysAsp: 0.614 ± 0.015	0.647CysGlu: 0.647 ± 0.014	0.545CysPhe: 0.545 ± 0.013	1.021CysGly: 1.021 ± 0.023	0.302CysHis: 0.302 ± 0.011	0.645CysIle: 0.645 ± 0.014	0.538CysLys: 0.538 ± 0.013	1.224CysLeu: 1.224 ± 0.02	0.28CysMet: 0.28 ± 0.01	0.395CysAsn: 0.395 ± 0.01	0.675CysPro: 0.675 ± 0.016	0.378CysGln: 0.378 ± 0.01	0.756CysArg: 0.756 ± 0.017	0.933CysSer: 0.933 ± 0.02	0.674CysThr: 0.674 ± 0.015	0.791CysVal: 0.791 ± 0.015	0.19CysTrp: 0.19 ± 0.007	0.366CysTyr: 0.366 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.726AspAla: 3.726 ± 0.038	0.606AspCys: 0.606 ± 0.013	3.755AspAsp: 3.755 ± 0.049	4.368AspGlu: 4.368 ± 0.043	2.009AspPhe: 2.009 ± 0.025	4.131AspGly: 4.131 ± 0.04	1.056AspHis: 1.056 ± 0.022	2.914AspIle: 2.914 ± 0.034	2.342AspLys: 2.342 ± 0.03	4.681AspLeu: 4.681 ± 0.038	1.165AspMet: 1.165 ± 0.019	1.796AspAsn: 1.796 ± 0.024	3.123AspPro: 3.123 ± 0.037	1.528AspGln: 1.528 ± 0.024	2.947AspArg: 2.947 ± 0.037	3.84AspSer: 3.84 ± 0.038	2.718AspThr: 2.718 ± 0.036	3.413AspVal: 3.413 ± 0.036	0.756AspTrp: 0.756 ± 0.018	1.533AspTyr: 1.533 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	5.26GluAla: 5.26 ± 0.062	0.702GluCys: 0.702 ± 0.017	4.313GluAsp: 4.313 ± 0.047	6.576GluGlu: 6.576 ± 0.08	2.141GluPhe: 2.141 ± 0.029	4.817GluGly: 4.817 ± 0.046	1.27GluHis: 1.27 ± 0.018	3.376GluIle: 3.376 ± 0.035	4.444GluLys: 4.444 ± 0.05	5.411GluLeu: 5.411 ± 0.047	1.637GluMet: 1.637 ± 0.021	2.43GluAsn: 2.43 ± 0.028	2.528GluPro: 2.528 ± 0.035	2.123GluGln: 2.123 ± 0.03	4.475GluArg: 4.475 ± 0.05	4.179GluSer: 4.179 ± 0.042	3.253GluThr: 3.253 ± 0.037	4.152GluVal: 4.152 ± 0.041	0.884GluTrp: 0.884 ± 0.018	1.756GluTyr: 1.756 ± 0.025	0.002GluXaa: 0.002 ± 0.001
Phe	2.763PheAla: 2.763 ± 0.036	0.576PheCys: 0.576 ± 0.015	2.052PheAsp: 2.052 ± 0.027	2.156PheGlu: 2.156 ± 0.026	1.647PhePhe: 1.647 ± 0.029	2.848PheGly: 2.848 ± 0.046	0.864PheHis: 0.864 ± 0.017	1.717PheIle: 1.717 ± 0.029	1.552PheLys: 1.552 ± 0.025	3.615PheLeu: 3.615 ± 0.04	0.756PheMet: 0.756 ± 0.015	1.293PheAsn: 1.293 ± 0.019	2.039PhePro: 2.039 ± 0.027	1.22PheGln: 1.22 ± 0.022	2.01PheArg: 2.01 ± 0.026	3.124PheSer: 3.124 ± 0.034	2.116PheThr: 2.116 ± 0.028	2.398PheVal: 2.398 ± 0.031	0.548PheTrp: 0.548 ± 0.012	1.096PheTyr: 1.096 ± 0.021	0.001PheXaa: 0.001 ± 0.0
Gly	5.416GlyAla: 5.416 ± 0.046	0.901GlyCys: 0.901 ± 0.018	3.971GlyAsp: 3.971 ± 0.037	4.773GlyGlu: 4.773 ± 0.049	2.845GlyPhe: 2.845 ± 0.035	8.087GlyGly: 8.087 ± 0.087	1.514GlyHis: 1.514 ± 0.029	3.769GlyIle: 3.769 ± 0.037	4.167GlyLys: 4.167 ± 0.043	5.982GlyLeu: 5.982 ± 0.046	1.777GlyMet: 1.777 ± 0.024	2.78GlyAsn: 2.78 ± 0.033	3.225GlyPro: 3.225 ± 0.045	2.223GlyGln: 2.223 ± 0.029	4.838GlyArg: 4.838 ± 0.045	6.201GlySer: 6.201 ± 0.066	3.951GlyThr: 3.951 ± 0.039	5.116GlyVal: 5.116 ± 0.047	1.171GlyTrp: 1.171 ± 0.021	2.169GlyTyr: 2.169 ± 0.028	0.001GlyXaa: 0.001 ± 0.0
His	1.547HisAla: 1.547 ± 0.025	0.3HisCys: 0.3 ± 0.011	1.07HisAsp: 1.07 ± 0.019	1.219HisGlu: 1.219 ± 0.021	0.877HisPhe: 0.877 ± 0.018	1.59HisGly: 1.59 ± 0.025	0.971HisHis: 0.971 ± 0.026	1.167HisIle: 1.167 ± 0.019	0.967HisLys: 0.967 ± 0.017	2.21HisLeu: 2.21 ± 0.025	0.463HisMet: 0.463 ± 0.011	0.806HisAsn: 0.806 ± 0.016	1.815HisPro: 1.815 ± 0.031	0.984HisGln: 0.984 ± 0.021	1.57HisArg: 1.57 ± 0.031	1.952HisSer: 1.952 ± 0.032	1.284HisThr: 1.284 ± 0.024	1.275HisVal: 1.275 ± 0.021	0.275HisTrp: 0.275 ± 0.008	0.671HisTyr: 0.671 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	3.987IleAla: 3.987 ± 0.039	0.733IleCys: 0.733 ± 0.015	2.653IleAsp: 2.653 ± 0.035	3.016IleGlu: 3.016 ± 0.038	1.957IlePhe: 1.957 ± 0.029	3.256IleGly: 3.256 ± 0.034	1.21IleHis: 1.21 ± 0.021	2.51IleIle: 2.51 ± 0.035	2.376IleLys: 2.376 ± 0.027	4.67IleLeu: 4.67 ± 0.049	0.99IleMet: 0.99 ± 0.017	1.769IleAsn: 1.769 ± 0.026	3.249IlePro: 3.249 ± 0.032	1.697IleGln: 1.697 ± 0.027	2.934IleArg: 2.934 ± 0.037	4.164IleSer: 4.164 ± 0.036	2.994IleThr: 2.994 ± 0.031	3.209IleVal: 3.209 ± 0.04	0.656IleTrp: 0.656 ± 0.014	1.402IleTyr: 1.402 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.208LysAla: 4.208 ± 0.049	0.579LysCys: 0.579 ± 0.013	2.756LysAsp: 2.756 ± 0.03	3.978LysGlu: 3.978 ± 0.051	1.651LysPhe: 1.651 ± 0.023	3.567LysGly: 3.567 ± 0.036	1.162LysHis: 1.162 ± 0.021	2.588LysIle: 2.588 ± 0.032	3.998LysLys: 3.998 ± 0.054	4.355LysLeu: 4.355 ± 0.045	1.128LysMet: 1.128 ± 0.017	1.936LysAsn: 1.936 ± 0.026	2.889LysPro: 2.889 ± 0.044	1.759LysGln: 1.759 ± 0.025	3.943LysArg: 3.943 ± 0.045	3.744LysSer: 3.744 ± 0.042	2.94LysThr: 2.94 ± 0.032	3.185LysVal: 3.185 ± 0.037	0.663LysTrp: 0.663 ± 0.015	1.464LysTyr: 1.464 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	7.25LeuAla: 7.25 ± 0.046	1.18LeuCys: 1.18 ± 0.021	4.774LeuAsp: 4.774 ± 0.051	5.873LeuGlu: 5.873 ± 0.063	3.361LeuPhe: 3.361 ± 0.041	6.162LeuGly: 6.162 ± 0.062	2.166LeuHis: 2.166 ± 0.03	3.876LeuIle: 3.876 ± 0.044	4.664LeuLys: 4.664 ± 0.051	8.628LeuLeu: 8.628 ± 0.081	1.761LeuMet: 1.761 ± 0.025	3.012LeuAsn: 3.012 ± 0.032	5.582LeuPro: 5.582 ± 0.049	3.429LeuGln: 3.429 ± 0.039	5.817LeuArg: 5.817 ± 0.048	7.376LeuSer: 7.376 ± 0.064	4.808LeuThr: 4.808 ± 0.047	5.447LeuVal: 5.447 ± 0.051	1.107LeuTrp: 1.107 ± 0.025	2.278LeuTyr: 2.278 ± 0.028	0.003LeuXaa: 0.003 ± 0.001
Met	1.949MetAla: 1.949 ± 0.026	0.259MetCys: 0.259 ± 0.01	1.241MetAsp: 1.241 ± 0.02	1.493MetGlu: 1.493 ± 0.022	0.749MetPhe: 0.749 ± 0.016	1.642MetGly: 1.642 ± 0.021	0.457MetHis: 0.457 ± 0.015	1.027MetIle: 1.027 ± 0.017	1.106MetLys: 1.106 ± 0.019	1.777MetLeu: 1.777 ± 0.023	0.55MetMet: 0.55 ± 0.016	0.788MetAsn: 0.788 ± 0.017	1.21MetPro: 1.21 ± 0.019	0.724MetGln: 0.724 ± 0.016	1.271MetArg: 1.271 ± 0.02	1.825MetSer: 1.825 ± 0.026	1.172MetThr: 1.172 ± 0.017	1.372MetVal: 1.372 ± 0.021	0.252MetTrp: 0.252 ± 0.01	0.508MetTyr: 0.508 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.73AsnAla: 2.73 ± 0.027	0.451AsnCys: 0.451 ± 0.011	1.709AsnAsp: 1.709 ± 0.019	1.946AsnGlu: 1.946 ± 0.025	1.378AsnPhe: 1.378 ± 0.025	2.906AsnGly: 2.906 ± 0.037	0.861AsnHis: 0.861 ± 0.017	1.982AsnIle: 1.982 ± 0.026	1.65AsnLys: 1.65 ± 0.024	3.277AsnLeu: 3.277 ± 0.037	0.777AsnMet: 0.777 ± 0.016	1.537AsnAsn: 1.537 ± 0.028	2.577AsnPro: 2.577 ± 0.036	1.169AsnGln: 1.169 ± 0.021	2.075AsnArg: 2.075 ± 0.028	2.994AsnSer: 2.994 ± 0.036	2.171AsnThr: 2.171 ± 0.03	2.213AsnVal: 2.213 ± 0.027	0.494AsnTrp: 0.494 ± 0.013	1.027AsnTyr: 1.027 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	4.928ProAla: 4.928 ± 0.063	0.507ProCys: 0.507 ± 0.015	2.825ProAsp: 2.825 ± 0.034	3.78ProGlu: 3.78 ± 0.037	2.024ProPhe: 2.024 ± 0.029	4.146ProGly: 4.146 ± 0.045	1.486ProHis: 1.486 ± 0.021	2.725ProIle: 2.725 ± 0.032	2.91ProLys: 2.91 ± 0.035	4.927ProLeu: 4.927 ± 0.043	1.035ProMet: 1.035 ± 0.021	2.226ProAsn: 2.226 ± 0.028	6.933ProPro: 6.933 ± 0.115	2.449ProGln: 2.449 ± 0.041	3.518ProArg: 3.518 ± 0.044	6.122ProSer: 6.122 ± 0.071	4.477ProThr: 4.477 ± 0.056	3.597ProVal: 3.597 ± 0.04	0.581ProTrp: 0.581 ± 0.014	1.45ProTyr: 1.45 ± 0.025	0.002ProXaa: 0.002 ± 0.001
Gln	2.765GlnAla: 2.765 ± 0.033	0.37GlnCys: 0.37 ± 0.01	1.666GlnAsp: 1.666 ± 0.024	2.245GlnGlu: 2.245 ± 0.028	1.181GlnPhe: 1.181 ± 0.023	2.153GlnGly: 2.153 ± 0.032	0.955GlnHis: 0.955 ± 0.021	1.697GlnIle: 1.697 ± 0.026	1.975GlnLys: 1.975 ± 0.029	3.044GlnLeu: 3.044 ± 0.037	0.784GlnMet: 0.784 ± 0.017	1.437GlnAsn: 1.437 ± 0.024	2.211GlnPro: 2.211 ± 0.04	2.432GlnGln: 2.432 ± 0.07	2.39GlnArg: 2.39 ± 0.03	2.677GlnSer: 2.677 ± 0.03	2.058GlnThr: 2.058 ± 0.031	1.961GlnVal: 1.961 ± 0.029	0.42GlnTrp: 0.42 ± 0.013	0.97GlnTyr: 0.97 ± 0.02	0.001GlnXaa: 0.001 ± 0.001
Arg	4.765ArgAla: 4.765 ± 0.043	0.742ArgCys: 0.742 ± 0.018	3.252ArgAsp: 3.252 ± 0.037	4.535ArgGlu: 4.535 ± 0.046	2.107ArgPhe: 2.107 ± 0.025	4.546ArgGly: 4.546 ± 0.045	1.486ArgHis: 1.486 ± 0.022	3.146ArgIle: 3.146 ± 0.037	4.062ArgLys: 4.062 ± 0.042	5.335ArgLeu: 5.335 ± 0.05	1.492ArgMet: 1.492 ± 0.022	2.389ArgAsn: 2.389 ± 0.03	3.327ArgPro: 3.327 ± 0.038	2.322ArgGln: 2.322 ± 0.033	5.522ArgArg: 5.522 ± 0.065	4.932ArgSer: 4.932 ± 0.049	3.27ArgThr: 3.27 ± 0.036	3.79ArgVal: 3.79 ± 0.041	0.864ArgTrp: 0.864 ± 0.019	1.708ArgTyr: 1.708 ± 0.024	0.001ArgXaa: 0.001 ± 0.0
Ser	6.434SerAla: 6.434 ± 0.052	0.872SerCys: 0.872 ± 0.019	3.822SerAsp: 3.822 ± 0.041	4.251SerGlu: 4.251 ± 0.043	2.935SerPhe: 2.935 ± 0.028	6.226SerGly: 6.226 ± 0.061	1.904SerHis: 1.904 ± 0.029	3.982SerIle: 3.982 ± 0.042	3.732SerLys: 3.732 ± 0.038	7.344SerLeu: 7.344 ± 0.056	1.688SerMet: 1.688 ± 0.022	2.893SerAsn: 2.893 ± 0.035	6.288SerPro: 6.288 ± 0.082	2.845SerGln: 2.845 ± 0.038	5.134SerArg: 5.134 ± 0.054	9.112SerSer: 9.112 ± 0.1	5.633SerThr: 5.633 ± 0.058	4.554SerVal: 4.554 ± 0.044	0.956SerTrp: 0.956 ± 0.018	2.021SerTyr: 2.021 ± 0.028	0.001SerXaa: 0.001 ± 0.001
Thr	4.836ThrAla: 4.836 ± 0.044	0.701ThrCys: 0.701 ± 0.017	2.524ThrAsp: 2.524 ± 0.027	3.025ThrGlu: 3.025 ± 0.036	2.156ThrPhe: 2.156 ± 0.03	4.292ThrGly: 4.292 ± 0.049	1.335ThrHis: 1.335 ± 0.02	3.118ThrIle: 3.118 ± 0.032	2.686ThrLys: 2.686 ± 0.031	5.328ThrLeu: 5.328 ± 0.052	1.07ThrMet: 1.07 ± 0.019	2.035ThrAsn: 2.035 ± 0.024	4.809ThrPro: 4.809 ± 0.052	1.878ThrGln: 1.878 ± 0.027	3.267ThrArg: 3.267 ± 0.036	5.313ThrSer: 5.313 ± 0.058	4.423ThrThr: 4.423 ± 0.057	3.502ThrVal: 3.502 ± 0.037	0.696ThrTrp: 0.696 ± 0.014	1.518ThrTyr: 1.518 ± 0.024	0.001ThrXaa: 0.001 ± 0.001
Val	4.913ValAla: 4.913 ± 0.042	0.858ValCys: 0.858 ± 0.016	3.563ValAsp: 3.563 ± 0.041	4.396ValGlu: 4.396 ± 0.044	2.431ValPhe: 2.431 ± 0.034	4.644ValGly: 4.644 ± 0.041	1.363ValHis: 1.363 ± 0.022	3.068ValIle: 3.068 ± 0.035	3.228ValLys: 3.228 ± 0.036	5.726ValLeu: 5.726 ± 0.054	1.398ValMet: 1.398 ± 0.02	2.147ValAsn: 2.147 ± 0.028	3.469ValPro: 3.469 ± 0.043	2.039ValGln: 2.039 ± 0.026	3.728ValArg: 3.728 ± 0.037	4.608ValSer: 4.608 ± 0.041	3.405ValThr: 3.405 ± 0.039	4.597ValVal: 4.597 ± 0.045	0.842ValTrp: 0.842 ± 0.018	1.713ValTyr: 1.713 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	0.945TrpAla: 0.945 ± 0.018	0.186TrpCys: 0.186 ± 0.007	0.803TrpAsp: 0.803 ± 0.021	0.874TrpGlu: 0.874 ± 0.018	0.45TrpPhe: 0.45 ± 0.012	1.004TrpGly: 1.004 ± 0.021	0.272TrpHis: 0.272 ± 0.009	0.663TrpIle: 0.663 ± 0.015	0.799TrpLys: 0.799 ± 0.017	1.161TrpLeu: 1.161 ± 0.02	0.331TrpMet: 0.331 ± 0.01	0.531TrpAsn: 0.531 ± 0.013	0.466TrpPro: 0.466 ± 0.013	0.409TrpGln: 0.409 ± 0.01	0.921TrpArg: 0.921 ± 0.018	0.905TrpSer: 0.905 ± 0.019	0.727TrpThr: 0.727 ± 0.017	0.861TrpVal: 0.861 ± 0.018	0.265TrpTrp: 0.265 ± 0.01	0.363TrpTyr: 0.363 ± 0.011	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.898TyrAla: 1.898 ± 0.024	0.437TyrCys: 0.437 ± 0.014	1.535TyrAsp: 1.535 ± 0.025	1.549TyrGlu: 1.549 ± 0.023	1.181TyrPhe: 1.181 ± 0.021	1.911TyrGly: 1.911 ± 0.025	0.746TyrHis: 0.746 ± 0.015	1.383TyrIle: 1.383 ± 0.021	1.216TyrLys: 1.216 ± 0.039	2.597TyrLeu: 2.597 ± 0.031	0.551TyrMet: 0.551 ± 0.014	1.078TyrAsn: 1.078 ± 0.017	1.515TyrPro: 1.515 ± 0.024	0.994TyrGln: 0.994 ± 0.019	1.685TyrArg: 1.685 ± 0.026	2.124TyrSer: 2.124 ± 0.03	1.576TyrThr: 1.576 ± 0.027	1.553TyrVal: 1.553 ± 0.023	0.383TyrTrp: 0.383 ± 0.013	0.916TyrTyr: 0.916 ± 0.019	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.0	4.15XaaXaa: 4.15 ± 2.488

Statistics based on 7494 proteins (3279832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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