Lactobacillus kimchicus JCM 15530

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Secundilactobacillus; Secundilactobacillus kimchicus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2470 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0R1HWX8|A0A0R1HWX8_9LACO CSD domain-containing protein OS=Lactobacillus kimchicus JCM 15530 OX=1302272 GN=FC96_GL002087 PE=4 SV=1
MM1 pKa = 7.35TFSFLLFSSTFGFTVVGNTQAQATEE26 pKa = 4.17TPTAVVQNVKK36 pKa = 9.78TSTPKK41 pKa = 10.18AHH43 pKa = 6.62ATFTTSVKK51 pKa = 10.55SINQVVQADD60 pKa = 4.28DD61 pKa = 3.69YY62 pKa = 10.91ATAITQEE69 pKa = 4.36VNWINTQVAAEE80 pKa = 4.88KK81 pKa = 9.56LTGWDD86 pKa = 2.74ALALVRR92 pKa = 11.84SSTGITDD99 pKa = 3.52AQKK102 pKa = 8.67TQIQTNIEE110 pKa = 3.99TNYY113 pKa = 10.64ADD115 pKa = 5.49PSTQHH120 pKa = 5.95QPTDD124 pKa = 3.39YY125 pKa = 10.82ARR127 pKa = 11.84DD128 pKa = 3.91VIGLICVGADD138 pKa = 3.24PTNVNGQNLVQEE150 pKa = 4.51AVTNGIAADD159 pKa = 3.62ADD161 pKa = 4.39VYY163 pKa = 11.4AVTYY167 pKa = 10.45GLIAATAAQSSNLGNVQTADD187 pKa = 3.67LQIMVTNLLGMQNSEE202 pKa = 3.66TGYY205 pKa = 8.01WTDD208 pKa = 2.95QYY210 pKa = 11.52GYY212 pKa = 8.34TLDD215 pKa = 3.68TTGMALQALALYY227 pKa = 9.89LGNSEE232 pKa = 4.62FDD234 pKa = 3.07TDD236 pKa = 3.98GRR238 pKa = 11.84ITNAVNSAFNTITLIGGNGALQEE261 pKa = 4.79DD262 pKa = 5.54GNFKK266 pKa = 11.23DD267 pKa = 4.53PFVADD272 pKa = 3.55AAVNSSSDD280 pKa = 3.07AMLIAGLAACGTEE293 pKa = 5.09LPDD296 pKa = 4.23EE297 pKa = 4.85SPLSALLSFQVLHH310 pKa = 6.07GTDD313 pKa = 2.97AGSFNWTPEE322 pKa = 3.75MTYY325 pKa = 10.99DD326 pKa = 3.8RR327 pKa = 11.84QLSTQQAVYY336 pKa = 10.96ALDD339 pKa = 3.5QYY341 pKa = 11.57SYY343 pKa = 11.51SVDD346 pKa = 3.11GKK348 pKa = 11.43GSIFDD353 pKa = 3.65FTANPVNN360 pKa = 3.62

Molecular weight:
38.22 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0R1HMB6|A0A0R1HMB6_9LACO HTH arsR-type domain-containing protein OS=Lactobacillus kimchicus JCM 15530 OX=1302272 GN=FC96_GL002213 PE=4 SV=1
MM1 pKa = 7.45VYY3 pKa = 9.73VGKK6 pKa = 10.69RR7 pKa = 11.84IDD9 pKa = 3.54LTGNRR14 pKa = 11.84YY15 pKa = 9.13SRR17 pKa = 11.84LMVVSFVGNSKK28 pKa = 10.47NGNARR33 pKa = 11.84WLCEE37 pKa = 3.95CDD39 pKa = 2.74CGRR42 pKa = 11.84FIEE45 pKa = 4.35TDD47 pKa = 3.72GYY49 pKa = 10.46RR50 pKa = 11.84LRR52 pKa = 11.84TGRR55 pKa = 11.84VRR57 pKa = 11.84SCGCLRR63 pKa = 11.84RR64 pKa = 11.84EE65 pKa = 4.01KK66 pKa = 11.0SRR68 pKa = 11.84DD69 pKa = 3.32RR70 pKa = 11.84LLGFAATKK78 pKa = 10.43QNIGVADD85 pKa = 4.18NLPIHH90 pKa = 6.55NGTVLRR96 pKa = 11.84LTDD99 pKa = 3.86KK100 pKa = 10.92NNQSGVIGVSWDD112 pKa = 3.32KK113 pKa = 11.16QSQKK117 pKa = 8.88WVARR121 pKa = 11.84LMVRR125 pKa = 11.84GKK127 pKa = 10.43YY128 pKa = 9.42VINRR132 pKa = 11.84KK133 pKa = 7.83FHH135 pKa = 6.25NFNQAVSFRR144 pKa = 11.84HH145 pKa = 4.49QAEE148 pKa = 4.12LQFRR152 pKa = 11.84DD153 pKa = 3.39PLLRR157 pKa = 5.42

Molecular weight:
18.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2470

0

2470

740804

50

2467

299.9

33.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.248 ± 0.063

0.375 ± 0.01

5.822 ± 0.051

4.965 ± 0.053

4.198 ± 0.042

7.122 ± 0.05

2.172 ± 0.024

6.356 ± 0.045

5.357 ± 0.054

9.897 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.687 ± 0.03

4.332 ± 0.035

3.876 ± 0.028

4.665 ± 0.04

4.314 ± 0.036

5.861 ± 0.056

6.787 ± 0.052

7.597 ± 0.044

1.082 ± 0.018

3.287 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski