Lactobacillus kimchicus JCM 15530
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2470 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1HWX8|A0A0R1HWX8_9LACO CSD domain-containing protein OS=Lactobacillus kimchicus JCM 15530 OX=1302272 GN=FC96_GL002087 PE=4 SV=1
MM1 pKa = 7.35 TFSFLLFSSTFGFTVVGNTQAQATEE26 pKa = 4.17 TPTAVVQNVKK36 pKa = 9.78 TSTPKK41 pKa = 10.18 AHH43 pKa = 6.62 ATFTTSVKK51 pKa = 10.55 SINQVVQADD60 pKa = 4.28 DD61 pKa = 3.69 YY62 pKa = 10.91 ATAITQEE69 pKa = 4.36 VNWINTQVAAEE80 pKa = 4.88 KK81 pKa = 9.56 LTGWDD86 pKa = 2.74 ALALVRR92 pKa = 11.84 SSTGITDD99 pKa = 3.52 AQKK102 pKa = 8.67 TQIQTNIEE110 pKa = 3.99 TNYY113 pKa = 10.64 ADD115 pKa = 5.49 PSTQHH120 pKa = 5.95 QPTDD124 pKa = 3.39 YY125 pKa = 10.82 ARR127 pKa = 11.84 DD128 pKa = 3.91 VIGLICVGADD138 pKa = 3.24 PTNVNGQNLVQEE150 pKa = 4.51 AVTNGIAADD159 pKa = 3.62 ADD161 pKa = 4.39 VYY163 pKa = 11.4 AVTYY167 pKa = 10.45 GLIAATAAQSSNLGNVQTADD187 pKa = 3.67 LQIMVTNLLGMQNSEE202 pKa = 3.66 TGYY205 pKa = 8.01 WTDD208 pKa = 2.95 QYY210 pKa = 11.52 GYY212 pKa = 8.34 TLDD215 pKa = 3.68 TTGMALQALALYY227 pKa = 9.89 LGNSEE232 pKa = 4.62 FDD234 pKa = 3.07 TDD236 pKa = 3.98 GRR238 pKa = 11.84 ITNAVNSAFNTITLIGGNGALQEE261 pKa = 4.79 DD262 pKa = 5.54 GNFKK266 pKa = 11.23 DD267 pKa = 4.53 PFVADD272 pKa = 3.55 AAVNSSSDD280 pKa = 3.07 AMLIAGLAACGTEE293 pKa = 5.09 LPDD296 pKa = 4.23 EE297 pKa = 4.85 SPLSALLSFQVLHH310 pKa = 6.07 GTDD313 pKa = 2.97 AGSFNWTPEE322 pKa = 3.75 MTYY325 pKa = 10.99 DD326 pKa = 3.8 RR327 pKa = 11.84 QLSTQQAVYY336 pKa = 10.96 ALDD339 pKa = 3.5 QYY341 pKa = 11.57 SYY343 pKa = 11.51 SVDD346 pKa = 3.11 GKK348 pKa = 11.43 GSIFDD353 pKa = 3.65 FTANPVNN360 pKa = 3.62
Molecular weight: 38.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.948
Patrickios 0.693
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A0R1HMB6|A0A0R1HMB6_9LACO HTH arsR-type domain-containing protein OS=Lactobacillus kimchicus JCM 15530 OX=1302272 GN=FC96_GL002213 PE=4 SV=1
MM1 pKa = 7.45 VYY3 pKa = 9.73 VGKK6 pKa = 10.69 RR7 pKa = 11.84 IDD9 pKa = 3.54 LTGNRR14 pKa = 11.84 YY15 pKa = 9.13 SRR17 pKa = 11.84 LMVVSFVGNSKK28 pKa = 10.47 NGNARR33 pKa = 11.84 WLCEE37 pKa = 3.95 CDD39 pKa = 2.74 CGRR42 pKa = 11.84 FIEE45 pKa = 4.35 TDD47 pKa = 3.72 GYY49 pKa = 10.46 RR50 pKa = 11.84 LRR52 pKa = 11.84 TGRR55 pKa = 11.84 VRR57 pKa = 11.84 SCGCLRR63 pKa = 11.84 RR64 pKa = 11.84 EE65 pKa = 4.01 KK66 pKa = 11.0 SRR68 pKa = 11.84 DD69 pKa = 3.32 RR70 pKa = 11.84 LLGFAATKK78 pKa = 10.43 QNIGVADD85 pKa = 4.18 NLPIHH90 pKa = 6.55 NGTVLRR96 pKa = 11.84 LTDD99 pKa = 3.86 KK100 pKa = 10.92 NNQSGVIGVSWDD112 pKa = 3.32 KK113 pKa = 11.16 QSQKK117 pKa = 8.88 WVARR121 pKa = 11.84 LMVRR125 pKa = 11.84 GKK127 pKa = 10.43 YY128 pKa = 9.42 VINRR132 pKa = 11.84 KK133 pKa = 7.83 FHH135 pKa = 6.25 NFNQAVSFRR144 pKa = 11.84 HH145 pKa = 4.49 QAEE148 pKa = 4.12 LQFRR152 pKa = 11.84 DD153 pKa = 3.39 PLLRR157 pKa = 5.42
Molecular weight: 18.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.677
IPC_protein 10.555
Toseland 10.774
ProMoST 10.716
Dawson 10.862
Bjellqvist 10.599
Wikipedia 11.082
Rodwell 11.038
Grimsley 10.906
Solomon 11.008
Lehninger 10.965
Nozaki 10.774
DTASelect 10.584
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.804
Patrickios 10.76
IPC_peptide 11.008
IPC2_peptide 9.823
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2470
0
2470
740804
50
2467
299.9
33.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.248 ± 0.063
0.375 ± 0.01
5.822 ± 0.051
4.965 ± 0.053
4.198 ± 0.042
7.122 ± 0.05
2.172 ± 0.024
6.356 ± 0.045
5.357 ± 0.054
9.897 ± 0.093
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.687 ± 0.03
4.332 ± 0.035
3.876 ± 0.028
4.665 ± 0.04
4.314 ± 0.036
5.861 ± 0.056
6.787 ± 0.052
7.597 ± 0.044
1.082 ± 0.018
3.287 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here