Amino acid dipepetide frequency for Aeromonas sp. RU39B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.712AlaAla: 11.712 ± 0.135	1.326AlaCys: 1.326 ± 0.038	5.38AlaAsp: 5.38 ± 0.084	6.269AlaGlu: 6.269 ± 0.09	3.445AlaPhe: 3.445 ± 0.064	8.381AlaGly: 8.381 ± 0.103	2.169AlaHis: 2.169 ± 0.043	5.761AlaIle: 5.761 ± 0.081	3.852AlaLys: 3.852 ± 0.077	13.651AlaLeu: 13.651 ± 0.156	3.224AlaMet: 3.224 ± 0.053	2.998AlaAsn: 2.998 ± 0.054	4.144AlaPro: 4.144 ± 0.064	4.343AlaGln: 4.343 ± 0.065	6.659AlaArg: 6.659 ± 0.096	5.968AlaSer: 5.968 ± 0.081	4.886AlaThr: 4.886 ± 0.063	6.909AlaVal: 6.909 ± 0.081	1.412AlaTrp: 1.412 ± 0.034	2.235AlaTyr: 2.235 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.061CysAla: 1.061 ± 0.034	0.183CysCys: 0.183 ± 0.012	0.683CysAsp: 0.683 ± 0.026	0.661CysGlu: 0.661 ± 0.023	0.448CysPhe: 0.448 ± 0.019	1.107CysGly: 1.107 ± 0.037	0.419CysHis: 0.419 ± 0.021	0.501CysIle: 0.501 ± 0.02	0.308CysLys: 0.308 ± 0.018	1.165CysLeu: 1.165 ± 0.037	0.225CysMet: 0.225 ± 0.013	0.316CysAsn: 0.316 ± 0.017	0.551CysPro: 0.551 ± 0.023	0.53CysGln: 0.53 ± 0.022	0.763CysArg: 0.763 ± 0.024	0.716CysSer: 0.716 ± 0.024	0.442CysThr: 0.442 ± 0.022	0.725CysVal: 0.725 ± 0.026	0.183CysTrp: 0.183 ± 0.012	0.344CysTyr: 0.344 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.272AspAla: 5.272 ± 0.075	0.525AspCys: 0.525 ± 0.02	2.855AspAsp: 2.855 ± 0.056	3.868AspGlu: 3.868 ± 0.06	1.877AspPhe: 1.877 ± 0.042	4.157AspGly: 4.157 ± 0.074	1.248AspHis: 1.248 ± 0.033	2.825AspIle: 2.825 ± 0.052	2.174AspLys: 2.174 ± 0.05	5.436AspLeu: 5.436 ± 0.067	1.309AspMet: 1.309 ± 0.031	1.723AspAsn: 1.723 ± 0.037	2.423AspPro: 2.423 ± 0.048	2.018AspGln: 2.018 ± 0.044	2.806AspArg: 2.806 ± 0.052	2.72AspSer: 2.72 ± 0.055	2.495AspThr: 2.495 ± 0.054	3.597AspVal: 3.597 ± 0.061	0.849AspTrp: 0.849 ± 0.027	1.781AspTyr: 1.781 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.762GluAla: 5.762 ± 0.081	0.544GluCys: 0.544 ± 0.025	1.932GluAsp: 1.932 ± 0.046	3.333GluGlu: 3.333 ± 0.058	1.865GluPhe: 1.865 ± 0.039	3.816GluGly: 3.816 ± 0.061	1.623GluHis: 1.623 ± 0.037	2.907GluIle: 2.907 ± 0.054	2.297GluLys: 2.297 ± 0.053	7.338GluLeu: 7.338 ± 0.086	1.632GluMet: 1.632 ± 0.041	1.528GluAsn: 1.528 ± 0.037	2.498GluPro: 2.498 ± 0.044	4.487GluGln: 4.487 ± 0.075	4.72GluArg: 4.72 ± 0.075	3.038GluSer: 3.038 ± 0.057	2.442GluThr: 2.442 ± 0.046	4.155GluVal: 4.155 ± 0.068	0.809GluTrp: 0.809 ± 0.025	1.307GluTyr: 1.307 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.845PheAla: 3.845 ± 0.058	0.51PheCys: 0.51 ± 0.022	2.32PheAsp: 2.32 ± 0.043	1.947PheGlu: 1.947 ± 0.048	1.426PhePhe: 1.426 ± 0.043	3.177PheGly: 3.177 ± 0.054	0.846PheHis: 0.846 ± 0.027	2.027PheIle: 2.027 ± 0.044	1.222PheLys: 1.222 ± 0.037	3.147PheLeu: 3.147 ± 0.061	0.937PheMet: 0.937 ± 0.029	1.442PheAsn: 1.442 ± 0.036	1.356PhePro: 1.356 ± 0.033	1.017PheGln: 1.017 ± 0.027	1.684PheArg: 1.684 ± 0.035	2.538PheSer: 2.538 ± 0.05	2.022PheThr: 2.022 ± 0.046	2.586PheVal: 2.586 ± 0.048	0.545PheTrp: 0.545 ± 0.022	1.103PheTyr: 1.103 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	6.857GlyAla: 6.857 ± 0.09	1.078GlyCys: 1.078 ± 0.033	3.909GlyAsp: 3.909 ± 0.06	4.959GlyGlu: 4.959 ± 0.066	3.215GlyPhe: 3.215 ± 0.049	5.598GlyGly: 5.598 ± 0.095	2.004GlyHis: 2.004 ± 0.048	4.733GlyIle: 4.733 ± 0.074	3.564GlyLys: 3.564 ± 0.062	8.289GlyLeu: 8.289 ± 0.103	2.5GlyMet: 2.5 ± 0.045	2.423GlyAsn: 2.423 ± 0.045	2.218GlyPro: 2.218 ± 0.051	3.613GlyGln: 3.613 ± 0.064	4.612GlyArg: 4.612 ± 0.059	4.529GlySer: 4.529 ± 0.089	3.561GlyThr: 3.561 ± 0.071	5.815GlyVal: 5.815 ± 0.075	1.369GlyTrp: 1.369 ± 0.039	2.379GlyTyr: 2.379 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.195HisAla: 2.195 ± 0.04	0.374HisCys: 0.374 ± 0.016	1.326HisAsp: 1.326 ± 0.035	1.251HisGlu: 1.251 ± 0.032	1.11HisPhe: 1.11 ± 0.031	2.023HisGly: 2.023 ± 0.048	0.898HisHis: 0.898 ± 0.03	1.184HisIle: 1.184 ± 0.032	0.749HisLys: 0.749 ± 0.023	2.869HisLeu: 2.869 ± 0.052	0.557HisMet: 0.557 ± 0.022	0.706HisAsn: 0.706 ± 0.023	1.581HisPro: 1.581 ± 0.038	1.282HisGln: 1.282 ± 0.031	1.386HisArg: 1.386 ± 0.031	1.421HisSer: 1.421 ± 0.038	1.157HisThr: 1.157 ± 0.028	1.3HisVal: 1.3 ± 0.032	0.48HisTrp: 0.48 ± 0.021	0.938HisTyr: 0.938 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.963IleAla: 5.963 ± 0.086	0.596IleCys: 0.596 ± 0.022	3.359IleAsp: 3.359 ± 0.052	3.449IleGlu: 3.449 ± 0.064	1.524IlePhe: 1.524 ± 0.036	4.562IleGly: 4.562 ± 0.07	1.172IleHis: 1.172 ± 0.029	2.508IleIle: 2.508 ± 0.053	2.128IleLys: 2.128 ± 0.048	4.514IleLeu: 4.514 ± 0.078	1.089IleMet: 1.089 ± 0.031	2.022IleAsn: 2.022 ± 0.05	2.319IlePro: 2.319 ± 0.047	1.544IleGln: 1.544 ± 0.037	2.961IleArg: 2.961 ± 0.052	3.339IleSer: 3.339 ± 0.052	3.066IleThr: 3.066 ± 0.053	3.244IleVal: 3.244 ± 0.061	0.554IleTrp: 0.554 ± 0.02	1.275IleTyr: 1.275 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.003LysAla: 4.003 ± 0.079	0.224LysCys: 0.224 ± 0.013	1.664LysAsp: 1.664 ± 0.038	2.205LysGlu: 2.205 ± 0.051	0.916LysPhe: 0.916 ± 0.029	2.774LysGly: 2.774 ± 0.049	0.764LysHis: 0.764 ± 0.022	1.677LysIle: 1.677 ± 0.044	1.563LysLys: 1.563 ± 0.05	3.798LysLeu: 3.798 ± 0.056	0.977LysMet: 0.977 ± 0.03	1.014LysAsn: 1.014 ± 0.036	1.933LysPro: 1.933 ± 0.041	1.845LysGln: 1.845 ± 0.04	2.212LysArg: 2.212 ± 0.043	2.094LysSer: 2.094 ± 0.052	1.794LysThr: 1.794 ± 0.042	3.099LysVal: 3.099 ± 0.057	0.368LysTrp: 0.368 ± 0.017	0.762LysTyr: 0.762 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	14.375LeuAla: 14.375 ± 0.149	1.603LeuCys: 1.603 ± 0.042	6.303LeuAsp: 6.303 ± 0.075	6.162LeuGlu: 6.162 ± 0.074	4.321LeuPhe: 4.321 ± 0.076	9.148LeuGly: 9.148 ± 0.11	2.505LeuHis: 2.505 ± 0.047	5.537LeuIle: 5.537 ± 0.071	4.068LeuLys: 4.068 ± 0.062	14.978LeuLeu: 14.978 ± 0.227	3.017LeuMet: 3.017 ± 0.052	3.243LeuAsn: 3.243 ± 0.054	6.472LeuPro: 6.472 ± 0.104	4.603LeuGln: 4.603 ± 0.072	6.729LeuArg: 6.729 ± 0.084	7.513LeuSer: 7.513 ± 0.096	6.662LeuThr: 6.662 ± 0.1	8.287LeuVal: 8.287 ± 0.099	1.534LeuTrp: 1.534 ± 0.045	2.608LeuTyr: 2.608 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	3.031MetAla: 3.031 ± 0.053	0.174MetCys: 0.174 ± 0.014	1.2MetAsp: 1.2 ± 0.029	1.261MetGlu: 1.261 ± 0.033	0.718MetPhe: 0.718 ± 0.026	2.018MetGly: 2.018 ± 0.043	0.492MetHis: 0.492 ± 0.021	1.226MetIle: 1.226 ± 0.034	1.089MetLys: 1.089 ± 0.032	3.254MetLeu: 3.254 ± 0.058	0.825MetMet: 0.825 ± 0.028	0.95MetAsn: 0.95 ± 0.031	1.313MetPro: 1.313 ± 0.033	1.276MetGln: 1.276 ± 0.031	1.412MetArg: 1.412 ± 0.031	1.857MetSer: 1.857 ± 0.036	1.731MetThr: 1.731 ± 0.041	2.011MetVal: 2.011 ± 0.042	0.239MetTrp: 0.239 ± 0.015	0.408MetTyr: 0.408 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.852AsnAla: 2.852 ± 0.044	0.271AsnCys: 0.271 ± 0.013	1.506AsnAsp: 1.506 ± 0.041	1.535AsnGlu: 1.535 ± 0.038	1.015AsnPhe: 1.015 ± 0.031	2.385AsnGly: 2.385 ± 0.052	0.726AsnHis: 0.726 ± 0.024	1.583AsnIle: 1.583 ± 0.039	1.089AsnLys: 1.089 ± 0.033	3.459AsnLeu: 3.459 ± 0.055	0.728AsnMet: 0.728 ± 0.026	0.989AsnAsn: 0.989 ± 0.03	1.964AsnPro: 1.964 ± 0.037	1.53AsnGln: 1.53 ± 0.037	1.869AsnArg: 1.869 ± 0.041	1.511AsnSer: 1.511 ± 0.039	1.465AsnThr: 1.465 ± 0.038	1.855AsnVal: 1.855 ± 0.042	0.502AsnTrp: 0.502 ± 0.022	0.833AsnTyr: 0.833 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	5.141ProAla: 5.141 ± 0.075	0.453ProCys: 0.453 ± 0.021	2.831ProAsp: 2.831 ± 0.052	3.067ProGlu: 3.067 ± 0.052	1.818ProPhe: 1.818 ± 0.037	3.363ProGly: 3.363 ± 0.05	1.174ProHis: 1.174 ± 0.032	2.05ProIle: 2.05 ± 0.04	1.466ProLys: 1.466 ± 0.037	5.714ProLeu: 5.714 ± 0.085	1.189ProMet: 1.189 ± 0.032	1.154ProAsn: 1.154 ± 0.036	1.605ProPro: 1.605 ± 0.044	1.885ProGln: 1.885 ± 0.041	2.181ProArg: 2.181 ± 0.046	2.478ProSer: 2.478 ± 0.041	2.308ProThr: 2.308 ± 0.039	3.796ProVal: 3.796 ± 0.056	0.787ProTrp: 0.787 ± 0.03	1.213ProTyr: 1.213 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.315GlnAla: 5.315 ± 0.08	0.417GlnCys: 0.417 ± 0.02	1.954GlnAsp: 1.954 ± 0.043	2.312GlnGlu: 2.312 ± 0.048	1.438GlnPhe: 1.438 ± 0.032	3.655GlnGly: 3.655 ± 0.055	1.316GlnHis: 1.316 ± 0.035	2.182GlnIle: 2.182 ± 0.042	1.38GlnLys: 1.38 ± 0.04	5.734GlnLeu: 5.734 ± 0.082	1.21GlnMet: 1.21 ± 0.035	1.022GlnAsn: 1.022 ± 0.027	2.269GlnPro: 2.269 ± 0.052	3.175GlnGln: 3.175 ± 0.075	3.128GlnArg: 3.128 ± 0.064	2.714GlnSer: 2.714 ± 0.059	2.192GlnThr: 2.192 ± 0.042	3.493GlnVal: 3.493 ± 0.064	0.79GlnTrp: 0.79 ± 0.028	0.971GlnTyr: 0.971 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	5.438ArgAla: 5.438 ± 0.079	0.63ArgCys: 0.63 ± 0.025	3.317ArgAsp: 3.317 ± 0.049	4.102ArgGlu: 4.102 ± 0.07	2.7ArgPhe: 2.7 ± 0.052	3.678ArgGly: 3.678 ± 0.059	2.065ArgHis: 2.065 ± 0.052	3.51ArgIle: 3.51 ± 0.057	1.954ArgLys: 1.954 ± 0.042	7.747ArgLeu: 7.747 ± 0.106	1.521ArgMet: 1.521 ± 0.035	1.741ArgAsn: 1.741 ± 0.036	2.52ArgPro: 2.52 ± 0.051	3.361ArgGln: 3.361 ± 0.063	4.079ArgArg: 4.079 ± 0.063	3.336ArgSer: 3.336 ± 0.059	2.538ArgThr: 2.538 ± 0.045	4.024ArgVal: 4.024 ± 0.058	1.09ArgTrp: 1.09 ± 0.034	2.087ArgTyr: 2.087 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	5.992SerAla: 5.992 ± 0.086	0.606SerCys: 0.606 ± 0.031	3.284SerAsp: 3.284 ± 0.068	3.431SerGlu: 3.431 ± 0.057	2.15SerPhe: 2.15 ± 0.051	5.266SerGly: 5.266 ± 0.083	1.628SerHis: 1.628 ± 0.037	2.712SerIle: 2.712 ± 0.051	1.891SerLys: 1.891 ± 0.043	7.116SerLeu: 7.116 ± 0.094	1.453SerMet: 1.453 ± 0.035	1.684SerAsn: 1.684 ± 0.043	2.504SerPro: 2.504 ± 0.051	2.726SerGln: 2.726 ± 0.049	3.847SerArg: 3.847 ± 0.065	3.529SerSer: 3.529 ± 0.084	2.685SerThr: 2.685 ± 0.051	4.061SerVal: 4.061 ± 0.078	0.949SerTrp: 0.949 ± 0.031	1.484SerTyr: 1.484 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.843ThrAla: 4.843 ± 0.061	0.471ThrCys: 0.471 ± 0.021	2.433ThrAsp: 2.433 ± 0.05	2.396ThrGlu: 2.396 ± 0.048	1.718ThrPhe: 1.718 ± 0.04	4.129ThrGly: 4.129 ± 0.069	1.184ThrHis: 1.184 ± 0.03	2.557ThrIle: 2.557 ± 0.055	1.275ThrLys: 1.275 ± 0.033	7.751ThrLeu: 7.751 ± 0.107	1.039ThrMet: 1.039 ± 0.032	1.284ThrAsn: 1.284 ± 0.033	3.021ThrPro: 3.021 ± 0.049	1.937ThrGln: 1.937 ± 0.04	3.151ThrArg: 3.151 ± 0.048	2.755ThrSer: 2.755 ± 0.063	2.63ThrThr: 2.63 ± 0.064	3.509ThrVal: 3.509 ± 0.072	0.61ThrTrp: 0.61 ± 0.021	1.139ThrTyr: 1.139 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	7.828ValAla: 7.828 ± 0.084	0.823ValCys: 0.823 ± 0.026	3.741ValAsp: 3.741 ± 0.062	4.084ValGlu: 4.084 ± 0.055	2.367ValPhe: 2.367 ± 0.045	5.06ValGly: 5.06 ± 0.066	1.372ValHis: 1.372 ± 0.033	4.035ValIle: 4.035 ± 0.053	2.551ValLys: 2.551 ± 0.054	7.854ValLeu: 7.854 ± 0.084	2.147ValMet: 2.147 ± 0.045	2.26ValAsn: 2.26 ± 0.043	2.919ValPro: 2.919 ± 0.051	2.466ValGln: 2.466 ± 0.048	4.173ValArg: 4.173 ± 0.061	4.662ValSer: 4.662 ± 0.078	4.163ValThr: 4.163 ± 0.079	5.658ValVal: 5.658 ± 0.079	0.88ValTrp: 0.88 ± 0.026	1.614ValTyr: 1.614 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.995TrpAla: 0.995 ± 0.03	0.191TrpCys: 0.191 ± 0.013	0.596TrpAsp: 0.596 ± 0.023	0.575TrpGlu: 0.575 ± 0.022	0.609TrpPhe: 0.609 ± 0.021	0.893TrpGly: 0.893 ± 0.032	0.44TrpHis: 0.44 ± 0.018	0.632TrpIle: 0.632 ± 0.026	0.339TrpLys: 0.339 ± 0.017	2.586TrpLeu: 2.586 ± 0.058	0.381TrpMet: 0.381 ± 0.018	0.377TrpAsn: 0.377 ± 0.018	0.699TrpPro: 0.699 ± 0.024	1.352TrpGln: 1.352 ± 0.039	1.055TrpArg: 1.055 ± 0.032	0.874TrpSer: 0.874 ± 0.026	0.47TrpThr: 0.47 ± 0.02	0.961TrpVal: 0.961 ± 0.028	0.282TrpTrp: 0.282 ± 0.015	0.347TrpTyr: 0.347 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.246TyrAla: 2.246 ± 0.043	0.333TyrCys: 0.333 ± 0.019	1.366TyrAsp: 1.366 ± 0.039	1.164TyrGlu: 1.164 ± 0.03	1.018TyrPhe: 1.018 ± 0.032	2.034TyrGly: 2.034 ± 0.045	0.743TyrHis: 0.743 ± 0.023	1.044TyrIle: 1.044 ± 0.033	0.726TyrLys: 0.726 ± 0.027	3.271TyrLeu: 3.271 ± 0.054	0.523TyrMet: 0.523 ± 0.02	0.788TyrAsn: 0.788 ± 0.03	1.363TyrPro: 1.363 ± 0.03	1.676TyrGln: 1.676 ± 0.045	2.012TyrArg: 2.012 ± 0.04	1.406TyrSer: 1.406 ± 0.039	1.123TyrThr: 1.123 ± 0.034	1.571TyrVal: 1.571 ± 0.036	0.421TyrTrp: 0.421 ± 0.018	0.805TyrTyr: 0.805 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3949 proteins (1259776 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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