Halomonas subglaciescola

Taxonomy: cellular organisms; Bacteria; Proteobacteria;

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M7I4F7|A0A1M7I4F7_9GAMM tRNA-dihydrouridine(16) synthase OS=Halomonas subglaciescola OX=29571 GN=dusC PE=3 SV=1
MM1 pKa = 7.07TFVVTEE7 pKa = 3.71NCIQCKK13 pKa = 8.52HH14 pKa = 5.47TDD16 pKa = 3.67CVEE19 pKa = 4.01VCPVDD24 pKa = 4.13CFYY27 pKa = 11.12EE28 pKa = 4.9GPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.7EE39 pKa = 5.82CIDD42 pKa = 4.15CALCEE47 pKa = 4.23PEE49 pKa = 4.78CPVDD53 pKa = 4.78AIYY56 pKa = 10.97SEE58 pKa = 5.35DD59 pKa = 4.09EE60 pKa = 4.08LPKK63 pKa = 10.73GQEE66 pKa = 3.56QFIEE70 pKa = 4.24INADD74 pKa = 4.27LAVEE78 pKa = 4.5WPNITEE84 pKa = 4.6KK85 pKa = 10.8KK86 pKa = 10.52DD87 pKa = 3.62PLPDD91 pKa = 3.85AEE93 pKa = 4.28EE94 pKa = 4.12WDD96 pKa = 4.01GKK98 pKa = 10.22SGKK101 pKa = 9.1IDD103 pKa = 3.19KK104 pKa = 10.91LEE106 pKa = 3.8RR107 pKa = 5.03

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7HXM2|A0A1M7HXM2_9GAMM Coproporphyrinogen-III oxidase OS=Halomonas subglaciescola OX=29571 GN=SAMN05878437_2412 PE=3 SV=1
MM1 pKa = 7.81AKK3 pKa = 10.08RR4 pKa = 11.84SRR6 pKa = 11.84RR7 pKa = 11.84GATRR11 pKa = 11.84RR12 pKa = 11.84GRR14 pKa = 11.84TTRR17 pKa = 11.84PVPAGGRR24 pKa = 11.84LDD26 pKa = 3.22RR27 pKa = 11.84WLNVAVTVAVITALVVGAAAVLAHH51 pKa = 6.68WLLL54 pKa = 4.01

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2724

0

2724

880880

30

2466

323.4

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.54 ± 0.057

0.935 ± 0.015

5.904 ± 0.044

6.021 ± 0.045

3.48 ± 0.031

7.922 ± 0.046

2.471 ± 0.025

4.607 ± 0.036

3.04 ± 0.038

11.207 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.025

2.795 ± 0.024

4.86 ± 0.034

4.05 ± 0.03

6.921 ± 0.038

5.482 ± 0.033

5.261 ± 0.024

7.097 ± 0.038

1.437 ± 0.022

2.433 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski