Aliiroseovarius pelagivivens
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3215 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R8ARA5|A0A2R8ARA5_9RHOB DNA-directed RNA polymerase subunit omega OS=Aliiroseovarius pelagivivens OX=1639690 GN=rpoZ PE=3 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 SLRR5 pKa = 11.84 PTLLSIPIIAATALPLASHH24 pKa = 6.33 PHH26 pKa = 4.8 VFIDD30 pKa = 4.57 AGANLLFDD38 pKa = 5.51 DD39 pKa = 5.3 AGQLAAVRR47 pKa = 11.84 VFWAYY52 pKa = 10.98 DD53 pKa = 3.32 EE54 pKa = 5.34 FYY56 pKa = 11.3 SLLLVEE62 pKa = 5.99 DD63 pKa = 4.41 NGLDD67 pKa = 3.58 ADD69 pKa = 4.36 GDD71 pKa = 4.15 GTPEE75 pKa = 3.72 QAALDD80 pKa = 4.15 AYY82 pKa = 10.5 AGQDD86 pKa = 3.45 VDD88 pKa = 3.55 WAAGFPGDD96 pKa = 5.97 LYY98 pKa = 11.34 LQDD101 pKa = 4.28 KK102 pKa = 10.55 GSDD105 pKa = 3.48 VALLGPVEE113 pKa = 3.97 HH114 pKa = 6.64 GMRR117 pKa = 11.84 YY118 pKa = 9.31 EE119 pKa = 4.38 DD120 pKa = 3.52 GRR122 pKa = 11.84 VISWHH127 pKa = 5.65 IRR129 pKa = 11.84 PLEE132 pKa = 3.97 TRR134 pKa = 11.84 VSVGAEE140 pKa = 3.67 PLLVQIYY147 pKa = 10.62 DD148 pKa = 3.36 PTFFVAYY155 pKa = 9.8 DD156 pKa = 3.2 VRR158 pKa = 11.84 LPVTVKK164 pKa = 10.58 GPEE167 pKa = 4.16 GCTVEE172 pKa = 5.91 HH173 pKa = 7.05 IPADD177 pKa = 3.77 LNAAYY182 pKa = 10.3 DD183 pKa = 3.83 KK184 pKa = 11.55 VEE186 pKa = 4.02 SLLYY190 pKa = 10.89 GPDD193 pKa = 3.27 SVEE196 pKa = 4.2 YY197 pKa = 10.87 SDD199 pKa = 5.79 DD200 pKa = 3.87 SYY202 pKa = 12.03 PEE204 pKa = 4.06 VGDD207 pKa = 4.43 LFADD211 pKa = 3.78 QLLLTCSEE219 pKa = 4.37
Molecular weight: 23.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.923
IPC_protein 3.923
Toseland 3.706
ProMoST 4.062
Dawson 3.923
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.617
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.05
Patrickios 1.214
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|A0A2R8AN06|A0A2R8AN06_9RHOB Urease accessory protein UreG OS=Aliiroseovarius pelagivivens OX=1639690 GN=ureG PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3215
0
3215
1011528
31
3616
314.6
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.457 ± 0.051
0.882 ± 0.014
6.318 ± 0.04
5.988 ± 0.039
3.853 ± 0.028
8.649 ± 0.072
2.129 ± 0.022
5.408 ± 0.033
3.682 ± 0.03
9.817 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.941 ± 0.023
2.893 ± 0.023
4.858 ± 0.036
3.257 ± 0.026
6.022 ± 0.039
5.417 ± 0.035
5.507 ± 0.031
7.288 ± 0.033
1.366 ± 0.019
2.264 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here