Sewage-associated circular DNA virus-6

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J182|A0A075J182_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-6 OX=1519395 PE=4 SV=1
MM1 pKa = 7.72GEE3 pKa = 3.82ARR5 pKa = 11.84RR6 pKa = 11.84QGVYY10 pKa = 9.01WLLTIPHH17 pKa = 7.22ADD19 pKa = 3.06WTPYY23 pKa = 10.26LPPGITWIRR32 pKa = 11.84GQLEE36 pKa = 4.31SGSDD40 pKa = 2.91TGYY43 pKa = 10.8LHH45 pKa = 5.96WQIIVGCSKK54 pKa = 10.44KK55 pKa = 10.32KK56 pKa = 9.55SLRR59 pKa = 11.84GIQEE63 pKa = 3.58IFGRR67 pKa = 11.84TCHH70 pKa = 6.65AEE72 pKa = 3.9LGRR75 pKa = 11.84SEE77 pKa = 4.61AAAAYY82 pKa = 8.71VWKK85 pKa = 10.47EE86 pKa = 3.68NTRR89 pKa = 11.84VEE91 pKa = 4.35GTQFEE96 pKa = 4.64LGKK99 pKa = 10.79KK100 pKa = 8.68PFKK103 pKa = 10.59RR104 pKa = 11.84NCATDD109 pKa = 3.01WDD111 pKa = 5.31LIWEE115 pKa = 4.31SAQRR119 pKa = 11.84GDD121 pKa = 4.48FMSIPADD128 pKa = 3.38IRR130 pKa = 11.84IHH132 pKa = 6.14SYY134 pKa = 8.66RR135 pKa = 11.84TLRR138 pKa = 11.84AISADD143 pKa = 3.42YY144 pKa = 10.63AKK146 pKa = 9.89PIEE149 pKa = 4.21MEE151 pKa = 3.87RR152 pKa = 11.84TCYY155 pKa = 10.75VFVGPTGTGKK165 pKa = 9.99SRR167 pKa = 11.84RR168 pKa = 11.84AWSEE172 pKa = 3.55SGMEE176 pKa = 4.55AFCKK180 pKa = 10.59DD181 pKa = 3.58PNSKK185 pKa = 9.74FWCGYY190 pKa = 7.91SGQSRR195 pKa = 11.84VVIDD199 pKa = 3.65EE200 pKa = 3.67FRR202 pKa = 11.84GRR204 pKa = 11.84IDD206 pKa = 3.22VSHH209 pKa = 7.5LLRR212 pKa = 11.84WLDD215 pKa = 3.64RR216 pKa = 11.84YY217 pKa = 9.52PCNVEE222 pKa = 3.74IKK224 pKa = 10.13GSSVPLCAEE233 pKa = 4.31SFWITSNLDD242 pKa = 2.7VDD244 pKa = 3.38QWYY247 pKa = 9.46PEE249 pKa = 4.17LDD251 pKa = 3.98EE252 pKa = 4.66PTLAALKK259 pKa = 10.14RR260 pKa = 11.84RR261 pKa = 11.84LKK263 pKa = 8.97ITHH266 pKa = 6.4FRR268 pKa = 11.84TLQQ271 pKa = 3.2

Molecular weight: 31.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J182|A0A075J182_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-6 OX=1519395 PE=4 SV=1
MM1 pKa = 6.12VTIRR5 pKa = 11.84EE6 pKa = 4.25LAGGLSGGALGYY18 pKa = 8.29ITHH21 pKa = 7.17GARR24 pKa = 11.84GAIKK28 pKa = 10.13GASAGYY34 pKa = 10.34ALARR38 pKa = 11.84NKK40 pKa = 10.22RR41 pKa = 11.84KK42 pKa = 10.44AEE44 pKa = 3.95DD45 pKa = 3.53DD46 pKa = 3.3MAGNRR51 pKa = 11.84KK52 pKa = 8.22YY53 pKa = 11.24VKK55 pKa = 9.48TKK57 pKa = 10.52HH58 pKa = 6.47PYY60 pKa = 8.4TKK62 pKa = 10.55VNKK65 pKa = 9.73LSKK68 pKa = 10.47KK69 pKa = 9.59YY70 pKa = 9.98GLHH73 pKa = 6.2RR74 pKa = 11.84ARR76 pKa = 11.84NISGGGPHH84 pKa = 7.82PYY86 pKa = 10.66NKK88 pKa = 9.91VGSNASAVKK97 pKa = 10.47LKK99 pKa = 10.35RR100 pKa = 11.84KK101 pKa = 9.95KK102 pKa = 10.33GVEE105 pKa = 3.29IKK107 pKa = 9.8KK108 pKa = 9.4RR109 pKa = 11.84KK110 pKa = 8.36RR111 pKa = 11.84VRR113 pKa = 11.84VSKK116 pKa = 10.8LFEE119 pKa = 4.52KK120 pKa = 10.47KK121 pKa = 10.0VKK123 pKa = 9.69QALEE127 pKa = 4.17AEE129 pKa = 4.97DD130 pKa = 4.86IEE132 pKa = 4.6GTYY135 pKa = 10.95LKK137 pKa = 9.49VTYY140 pKa = 11.01ARR142 pKa = 11.84MLPPASDD149 pKa = 3.3TSQAVIDD156 pKa = 3.96FANAFGPMEE165 pKa = 4.44FVDD168 pKa = 4.05AADD171 pKa = 3.5VLFNDD176 pKa = 4.02QVPVEE181 pKa = 4.92APTFPNNDD189 pKa = 2.92WSNNWIRR196 pKa = 11.84KK197 pKa = 7.21DD198 pKa = 3.49TVINSTMQLEE208 pKa = 4.69MKK210 pKa = 10.59NMSQRR215 pKa = 11.84TYY217 pKa = 7.81TLKK220 pKa = 10.35IYY222 pKa = 8.64TCQPKK227 pKa = 9.63VVPTDD232 pKa = 3.46AAPNDD237 pKa = 4.1ALADD241 pKa = 3.72WSTGMIMSTSFGANPLSNSPSTLYY265 pKa = 10.54CIPTDD270 pKa = 3.22APQFNRR276 pKa = 11.84FWKK279 pKa = 9.16AQCEE283 pKa = 4.42TVVLQPGQSHH293 pKa = 6.38TYY295 pKa = 10.03FLQGPNDD302 pKa = 3.45YY303 pKa = 11.36VLDD306 pKa = 3.99YY307 pKa = 11.26KK308 pKa = 10.62KK309 pKa = 11.04LQMKK313 pKa = 10.26NIAAAPNWLIPYY325 pKa = 10.27AKK327 pKa = 9.07FTRR330 pKa = 11.84NVFVVGHH337 pKa = 6.41TDD339 pKa = 3.58LVTSTLAGAGRR350 pKa = 11.84FPSGGVGQGGIVFEE364 pKa = 4.07RR365 pKa = 11.84RR366 pKa = 11.84MFYY369 pKa = 10.96KK370 pKa = 9.82MKK372 pKa = 10.06CPASAGFQYY381 pKa = 8.45PTGAYY386 pKa = 8.89PVGDD390 pKa = 3.9TQQLDD395 pKa = 3.46QKK397 pKa = 10.83IPAKK401 pKa = 10.16VIKK404 pKa = 9.75FFPIGAAGSTEE415 pKa = 4.22DD416 pKa = 3.84VLEE419 pKa = 4.38EE420 pKa = 4.17NPLSIINPLDD430 pKa = 3.17

Molecular weight: 47.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

701

271

430

350.5

39.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.559 ± 1.089

1.712 ± 0.705

4.993 ± 0.098

4.708 ± 1.099

3.994 ± 0.173

7.989 ± 0.136

1.712 ± 0.285

5.421 ± 0.694

7.275 ± 1.199

6.847 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.458

4.137 ± 1.302

5.849 ± 0.598

3.709 ± 0.22

5.991 ± 1.418

6.277 ± 0.627

6.134 ± 0.131

5.991 ± 0.888

2.425 ± 1.138

3.994 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski