Bifidobacterium phage BadAargau2
Average proteome isoelectric point is 5.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6Y0H1|A0A6G6Y0H1_9CAUD Uncharacterized protein OS=Bifidobacterium phage BadAargau2 OX=2713242 GN=BAAR0010003c01_00012 PE=4 SV=1
MM1 pKa = 7.3 QAIKK5 pKa = 10.92 DD6 pKa = 3.99 EE7 pKa = 4.56 LTEE10 pKa = 4.4 SMDD13 pKa = 4.3 DD14 pKa = 3.73 AVEE17 pKa = 3.95 LASAVVADD25 pKa = 4.48 WSGHH29 pKa = 4.15 MDD31 pKa = 3.14 VTVRR35 pKa = 11.84 PEE37 pKa = 3.46 YY38 pKa = 9.26 MAIVVTCGPVRR49 pKa = 11.84 LWIKK53 pKa = 10.61 APYY56 pKa = 9.49 GSSLITAYY64 pKa = 10.59 FPVSGYY70 pKa = 11.16 AEE72 pKa = 4.16 SCDD75 pKa = 3.22 IEE77 pKa = 4.71 HH78 pKa = 6.58 EE79 pKa = 4.42 NKK81 pKa = 10.35 LGEE84 pKa = 4.68 YY85 pKa = 8.71 FTMIQSSDD93 pKa = 3.7 MEE95 pKa = 4.1 QLRR98 pKa = 11.84 YY99 pKa = 10.61 DD100 pKa = 3.07 IMNWIRR106 pKa = 11.84 VRR108 pKa = 11.84 LVMSCLL114 pKa = 3.45
Molecular weight: 12.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.108
IPC2_protein 4.431
IPC_protein 4.317
Toseland 4.151
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.164
Rodwell 4.164
Grimsley 4.075
Solomon 4.279
Lehninger 4.228
Nozaki 4.393
DTASelect 4.558
Thurlkill 4.177
EMBOSS 4.19
Sillero 4.431
Patrickios 1.99
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.344
Protein with the highest isoelectric point:
>tr|A0A6G6Y069|A0A6G6Y069_9CAUD Uncharacterized protein OS=Bifidobacterium phage BadAargau2 OX=2713242 GN=BAAR0010003c01_00020 PE=4 SV=1
MM1 pKa = 7.42 ARR3 pKa = 11.84 SKK5 pKa = 9.96 KK6 pKa = 4.7 TTKK9 pKa = 10.01 RR10 pKa = 11.84 QRR12 pKa = 11.84 QAAQRR17 pKa = 11.84 KK18 pKa = 8.17 RR19 pKa = 11.84 NAQIRR24 pKa = 11.84 SAQVARR30 pKa = 11.84 NAARR34 pKa = 11.84 NIGTINPTKK43 pKa = 10.73 VSVGTLNKK51 pKa = 10.26 LSRR54 pKa = 11.84 PQLEE58 pKa = 3.69 RR59 pKa = 11.84 LAQNVGNEE67 pKa = 4.09 VYY69 pKa = 10.08 DD70 pKa = 4.03 AKK72 pKa = 11.28 SSVQKK77 pKa = 9.98 YY78 pKa = 9.67 LRR80 pKa = 11.84 SKK82 pKa = 10.06 PYY84 pKa = 8.6 YY85 pKa = 8.63 HH86 pKa = 7.34 APPVSITKK94 pKa = 10.12 RR95 pKa = 11.84 EE96 pKa = 3.84 RR97 pKa = 11.84 EE98 pKa = 3.83 FASRR102 pKa = 11.84 KK103 pKa = 9.9 VPTDD107 pKa = 3.28 QQIAAAPPKK116 pKa = 9.7 KK117 pKa = 10.34 AKK119 pKa = 10.18 LLRR122 pKa = 11.84 QQKK125 pKa = 10.16 RR126 pKa = 11.84 KK127 pKa = 8.18 ITAARR132 pKa = 11.84 NKK134 pKa = 10.45 LEE136 pKa = 3.62 DD137 pKa = 3.23 WKK139 pKa = 10.93 RR140 pKa = 11.84 YY141 pKa = 7.12 NAQVMAGRR149 pKa = 11.84 TARR152 pKa = 11.84 DD153 pKa = 3.22 EE154 pKa = 4.28 RR155 pKa = 11.84 ADD157 pKa = 3.66 ALNGTGGKK165 pKa = 9.11 EE166 pKa = 3.85 SRR168 pKa = 11.84 TRR170 pKa = 11.84 DD171 pKa = 3.0 AGKK174 pKa = 10.58 RR175 pKa = 11.84 STTDD179 pKa = 2.48 IDD181 pKa = 3.53 HH182 pKa = 7.57 LDD184 pKa = 3.44 RR185 pKa = 11.84 YY186 pKa = 10.96 RR187 pKa = 11.84 NVLKK191 pKa = 10.81 NPQIVSKK198 pKa = 11.11 LSDD201 pKa = 3.47 DD202 pKa = 4.03 EE203 pKa = 4.04 LRR205 pKa = 11.84 KK206 pKa = 9.96 EE207 pKa = 3.5 IRR209 pKa = 11.84 ARR211 pKa = 11.84 TKK213 pKa = 10.49 DD214 pKa = 3.45 LKK216 pKa = 10.1 NAKK219 pKa = 9.91 NILEE223 pKa = 4.33 NGVGRR228 pKa = 11.84 ISEE231 pKa = 4.01 KK232 pKa = 10.86 DD233 pKa = 3.03 NAYY236 pKa = 10.36 VEE238 pKa = 4.48 RR239 pKa = 11.84 RR240 pKa = 11.84 LKK242 pKa = 11.0 AGFGQSALDD251 pKa = 3.47 GWRR254 pKa = 11.84 KK255 pKa = 9.63 LSRR258 pKa = 11.84 TEE260 pKa = 4.02 RR261 pKa = 11.84 YY262 pKa = 10.09 SLVNQTSLLNRR273 pKa = 11.84 IEE275 pKa = 4.6 FYY277 pKa = 10.88 SVYY280 pKa = 10.65 NEE282 pKa = 3.69 EE283 pKa = 4.03 THH285 pKa = 7.47 AYY287 pKa = 8.91 EE288 pKa = 4.68 PWDD291 pKa = 3.6 HH292 pKa = 7.17 DD293 pKa = 3.49 RR294 pKa = 11.84 KK295 pKa = 9.98 EE296 pKa = 4.0 QVKK299 pKa = 8.68 QEE301 pKa = 4.13 RR302 pKa = 11.84 SDD304 pKa = 3.04 IMSFIRR310 pKa = 11.84 NARR313 pKa = 11.84 NISNASLEE321 pKa = 4.16
Molecular weight: 36.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.721
IPC_protein 10.321
Toseland 10.745
ProMoST 10.365
Dawson 10.833
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.169
Grimsley 10.877
Solomon 10.921
Lehninger 10.891
Nozaki 10.716
DTASelect 10.496
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.76
Patrickios 10.877
IPC_peptide 10.935
IPC2_peptide 9.092
IPC2.peptide.svr19 8.782
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
5929
55
710
257.8
28.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.096 ± 0.587
0.978 ± 0.186
7.59 ± 0.503
4.992 ± 0.295
3.542 ± 0.339
6.831 ± 0.478
1.737 ± 0.213
5.768 ± 0.237
5.347 ± 0.472
6.612 ± 0.338
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.154 ± 0.286
6.409 ± 0.48
3.846 ± 0.326
4.284 ± 0.486
5.363 ± 0.55
6.814 ± 0.476
6.612 ± 0.451
5.65 ± 0.371
1.636 ± 0.145
4.739 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here