Aerococcus sanguinicola
Average proteome isoelectric point is 5.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1731 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0X8FAF9|A0A0X8FAF9_9LACT Phosphate acyltransferase OS=Aerococcus sanguinicola OX=119206 GN=plsX PE=3 SV=1
MM1 pKa = 7.01 YY2 pKa = 10.02 LYY4 pKa = 10.61 YY5 pKa = 10.76 AIFTPSAGQFAIEE18 pKa = 4.36 FPDD21 pKa = 3.57 LEE23 pKa = 4.66 GAFSCGEE30 pKa = 4.11 DD31 pKa = 3.42 MDD33 pKa = 4.38 EE34 pKa = 4.33 ALYY37 pKa = 9.03 MAKK40 pKa = 10.37 DD41 pKa = 3.81 LLEE44 pKa = 4.33 GWLITAEE51 pKa = 4.22 EE52 pKa = 4.38 EE53 pKa = 4.26 GDD55 pKa = 4.39 PIPAPSLPDD64 pKa = 3.87 DD65 pKa = 4.35 LLVPEE70 pKa = 5.38 DD71 pKa = 4.36 ALLLPIEE78 pKa = 4.5 VDD80 pKa = 3.24 LDD82 pKa = 3.62 QAKK85 pKa = 9.95 EE86 pKa = 3.75 KK87 pKa = 10.83 HH88 pKa = 5.87 FLSGEE93 pKa = 3.98
Molecular weight: 10.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.839
IPC2_protein 3.554
IPC_protein 3.49
Toseland 3.3
ProMoST 3.63
Dawson 3.478
Bjellqvist 3.706
Wikipedia 3.414
Rodwell 3.325
Grimsley 3.21
Solomon 3.452
Lehninger 3.401
Nozaki 3.617
DTASelect 3.77
Thurlkill 3.363
EMBOSS 3.427
Sillero 3.617
Patrickios 0.693
IPC_peptide 3.439
IPC2_peptide 3.579
IPC2.peptide.svr19 3.659
Protein with the highest isoelectric point:
>tr|A0A109RD23|A0A109RD23_9LACT Uncharacterized protein OS=Aerococcus sanguinicola OX=119206 GN=AWM72_00230 PE=4 SV=1
MM1 pKa = 7.57 SKK3 pKa = 10.53 LIEE6 pKa = 4.91 SITNEE11 pKa = 4.02 QLRR14 pKa = 11.84 SDD16 pKa = 3.86 IPDD19 pKa = 3.75 FRR21 pKa = 11.84 PGDD24 pKa = 3.85 TVRR27 pKa = 11.84 VHH29 pKa = 6.44 AKK31 pKa = 9.56 VVEE34 pKa = 4.12 GDD36 pKa = 3.52 RR37 pKa = 11.84 EE38 pKa = 3.98 RR39 pKa = 11.84 IQIFEE44 pKa = 4.07 GVVIKK49 pKa = 10.73 RR50 pKa = 11.84 RR51 pKa = 11.84 GQGISEE57 pKa = 4.71 TYY59 pKa = 7.99 TVRR62 pKa = 11.84 KK63 pKa = 8.91 ISSGVGVEE71 pKa = 3.96 RR72 pKa = 11.84 TFPVHH77 pKa = 5.78 TPRR80 pKa = 11.84 VDD82 pKa = 3.29 QIEE85 pKa = 4.64 VIRR88 pKa = 11.84 QGKK91 pKa = 7.21 VRR93 pKa = 11.84 RR94 pKa = 11.84 AKK96 pKa = 10.42 LYY98 pKa = 9.27 YY99 pKa = 10.44 LRR101 pKa = 11.84 DD102 pKa = 3.56 RR103 pKa = 11.84 HH104 pKa = 5.68 GKK106 pKa = 8.29 AARR109 pKa = 11.84 IAEE112 pKa = 4.31 RR113 pKa = 11.84 RR114 pKa = 11.84 RR115 pKa = 11.84 KK116 pKa = 9.39
Molecular weight: 13.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.75
IPC_protein 10.833
Toseland 11.023
ProMoST 11.067
Dawson 11.067
Bjellqvist 10.862
Wikipedia 11.374
Rodwell 11.14
Grimsley 11.111
Solomon 11.33
Lehninger 11.272
Nozaki 10.994
DTASelect 10.877
Thurlkill 11.008
EMBOSS 11.462
Sillero 11.023
Patrickios 10.877
IPC_peptide 11.345
IPC2_peptide 9.955
IPC2.peptide.svr19 8.808
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1731
0
1731
556294
51
3265
321.4
35.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.768 ± 0.082
0.685 ± 0.016
6.586 ± 0.057
7.065 ± 0.067
4.07 ± 0.048
6.986 ± 0.07
1.978 ± 0.029
6.538 ± 0.06
5.558 ± 0.068
10.064 ± 0.115
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.408 ± 0.035
3.837 ± 0.043
3.786 ± 0.053
5.142 ± 0.053
4.471 ± 0.051
5.776 ± 0.05
4.93 ± 0.061
6.685 ± 0.059
0.904 ± 0.021
3.762 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here