Amino acid dipepetide frequency for Sphingomonas sp. YR710

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.291AlaAla: 19.291 ± 0.185	1.054AlaCys: 1.054 ± 0.037	7.893AlaAsp: 7.893 ± 0.089	7.144AlaGlu: 7.144 ± 0.096	4.343AlaPhe: 4.343 ± 0.057	11.369AlaGly: 11.369 ± 0.134	2.391AlaHis: 2.391 ± 0.049	8.045AlaIle: 8.045 ± 0.092	4.042AlaLys: 4.042 ± 0.069	13.748AlaLeu: 13.748 ± 0.13	4.03AlaMet: 4.03 ± 0.064	3.22AlaAsn: 3.22 ± 0.052	6.131AlaPro: 6.131 ± 0.085	4.178AlaGln: 4.178 ± 0.067	9.682AlaArg: 9.682 ± 0.117	6.872AlaSer: 6.872 ± 0.108	6.976AlaThr: 6.976 ± 0.146	8.585AlaVal: 8.585 ± 0.096	1.543AlaTrp: 1.543 ± 0.033	2.6AlaTyr: 2.6 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.974CysAla: 0.974 ± 0.027	0.099CysCys: 0.099 ± 0.009	0.533CysAsp: 0.533 ± 0.019	0.363CysGlu: 0.363 ± 0.018	0.311CysPhe: 0.311 ± 0.014	0.875CysGly: 0.875 ± 0.031	0.233CysHis: 0.233 ± 0.016	0.347CysIle: 0.347 ± 0.017	0.163CysLys: 0.163 ± 0.011	0.676CysLeu: 0.676 ± 0.023	0.149CysMet: 0.149 ± 0.011	0.211CysAsn: 0.211 ± 0.014	0.459CysPro: 0.459 ± 0.021	0.222CysGln: 0.222 ± 0.014	0.609CysArg: 0.609 ± 0.024	0.439CysSer: 0.439 ± 0.018	0.416CysThr: 0.416 ± 0.021	0.543CysVal: 0.543 ± 0.022	0.12CysTrp: 0.12 ± 0.009	0.166CysTyr: 0.166 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.482AspAla: 7.482 ± 0.094	0.488AspCys: 0.488 ± 0.02	3.452AspAsp: 3.452 ± 0.06	3.189AspGlu: 3.189 ± 0.063	2.09AspPhe: 2.09 ± 0.043	5.628AspGly: 5.628 ± 0.084	1.482AspHis: 1.482 ± 0.037	3.359AspIle: 3.359 ± 0.057	1.628AspLys: 1.628 ± 0.042	5.894AspLeu: 5.894 ± 0.073	1.475AspMet: 1.475 ± 0.034	1.299AspAsn: 1.299 ± 0.034	3.811AspPro: 3.811 ± 0.06	1.84AspGln: 1.84 ± 0.043	5.235AspArg: 5.235 ± 0.072	2.54AspSer: 2.54 ± 0.044	2.554AspThr: 2.554 ± 0.062	4.247AspVal: 4.247 ± 0.061	1.008AspTrp: 1.008 ± 0.029	1.555AspTyr: 1.555 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	7.015GluAla: 7.015 ± 0.094	0.291GluCys: 0.291 ± 0.014	2.452GluAsp: 2.452 ± 0.049	2.356GluGlu: 2.356 ± 0.057	1.481GluPhe: 1.481 ± 0.036	3.917GluGly: 3.917 ± 0.061	1.13GluHis: 1.13 ± 0.033	3.016GluIle: 3.016 ± 0.055	1.767GluLys: 1.767 ± 0.041	4.685GluLeu: 4.685 ± 0.073	1.384GluMet: 1.384 ± 0.032	1.234GluAsn: 1.234 ± 0.031	2.39GluPro: 2.39 ± 0.044	1.992GluGln: 1.992 ± 0.04	4.392GluArg: 4.392 ± 0.068	2.3GluSer: 2.3 ± 0.042	2.836GluThr: 2.836 ± 0.05	3.101GluVal: 3.101 ± 0.057	0.674GluTrp: 0.674 ± 0.024	0.942GluTyr: 0.942 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.926PheAla: 4.926 ± 0.072	0.326PheCys: 0.326 ± 0.016	2.676PheAsp: 2.676 ± 0.05	1.849PheGlu: 1.849 ± 0.034	1.271PhePhe: 1.271 ± 0.036	3.793PheGly: 3.793 ± 0.068	0.755PheHis: 0.755 ± 0.025	1.501PheIle: 1.501 ± 0.041	0.837PheLys: 0.837 ± 0.029	3.049PheLeu: 3.049 ± 0.053	0.722PheMet: 0.722 ± 0.024	0.994PheAsn: 0.994 ± 0.031	1.491PhePro: 1.491 ± 0.036	0.933PheGln: 0.933 ± 0.026	2.23PheArg: 2.23 ± 0.046	2.074PheSer: 2.074 ± 0.04	2.019PheThr: 2.019 ± 0.038	2.647PheVal: 2.647 ± 0.05	0.469PheTrp: 0.469 ± 0.023	0.827PheTyr: 0.827 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.89GlyAla: 9.89 ± 0.118	0.847GlyCys: 0.847 ± 0.026	4.98GlyAsp: 4.98 ± 0.091	4.337GlyGlu: 4.337 ± 0.074	3.596GlyPhe: 3.596 ± 0.056	8.146GlyGly: 8.146 ± 0.154	1.995GlyHis: 1.995 ± 0.042	4.771GlyIle: 4.771 ± 0.065	3.052GlyLys: 3.052 ± 0.056	8.832GlyLeu: 8.832 ± 0.094	2.337GlyMet: 2.337 ± 0.047	2.357GlyAsn: 2.357 ± 0.082	3.612GlyPro: 3.612 ± 0.051	2.911GlyGln: 2.911 ± 0.057	6.546GlyArg: 6.546 ± 0.094	5.07GlySer: 5.07 ± 0.088	4.867GlyThr: 4.867 ± 0.089	6.123GlyVal: 6.123 ± 0.075	1.565GlyTrp: 1.565 ± 0.04	2.357GlyTyr: 2.357 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.465HisAla: 2.465 ± 0.04	0.213HisCys: 0.213 ± 0.012	1.388HisAsp: 1.388 ± 0.033	0.956HisGlu: 0.956 ± 0.029	0.87HisPhe: 0.87 ± 0.03	1.996HisGly: 1.996 ± 0.041	0.63HisHis: 0.63 ± 0.027	1.143HisIle: 1.143 ± 0.031	0.465HisLys: 0.465 ± 0.02	2.019HisLeu: 2.019 ± 0.049	0.55HisMet: 0.55 ± 0.022	0.494HisAsn: 0.494 ± 0.021	1.365HisPro: 1.365 ± 0.033	0.544HisGln: 0.544 ± 0.019	1.557HisArg: 1.557 ± 0.033	0.991HisSer: 0.991 ± 0.025	0.71HisThr: 0.71 ± 0.021	1.569HisVal: 1.569 ± 0.035	0.369HisTrp: 0.369 ± 0.019	0.577HisTyr: 0.577 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	8.936IleAla: 8.936 ± 0.088	0.506IleCys: 0.506 ± 0.02	4.546IleAsp: 4.546 ± 0.062	3.757IleGlu: 3.757 ± 0.064	1.871IlePhe: 1.871 ± 0.045	5.902IleGly: 5.902 ± 0.075	0.972IleHis: 0.972 ± 0.029	2.553IleIle: 2.553 ± 0.044	1.304IleLys: 1.304 ± 0.036	4.527IleLeu: 4.527 ± 0.069	1.061IleMet: 1.061 ± 0.029	1.467IleAsn: 1.467 ± 0.038	2.33IlePro: 2.33 ± 0.043	1.241IleGln: 1.241 ± 0.033	3.539IleArg: 3.539 ± 0.063	3.144IleSer: 3.144 ± 0.057	2.914IleThr: 2.914 ± 0.066	4.627IleVal: 4.627 ± 0.062	0.661IleTrp: 0.661 ± 0.026	1.108IleTyr: 1.108 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.099LysAla: 4.099 ± 0.065	0.148LysCys: 0.148 ± 0.011	1.454LysAsp: 1.454 ± 0.043	1.112LysGlu: 1.112 ± 0.032	0.807LysPhe: 0.807 ± 0.026	2.461LysGly: 2.461 ± 0.041	0.53LysHis: 0.53 ± 0.02	1.62LysIle: 1.62 ± 0.042	0.972LysLys: 0.972 ± 0.035	3.225LysLeu: 3.225 ± 0.057	0.817LysMet: 0.817 ± 0.028	0.705LysAsn: 0.705 ± 0.023	1.934LysPro: 1.934 ± 0.043	0.881LysGln: 0.881 ± 0.029	2.044LysArg: 2.044 ± 0.052	1.585LysSer: 1.585 ± 0.037	1.659LysThr: 1.659 ± 0.039	2.145LysVal: 2.145 ± 0.047	0.343LysTrp: 0.343 ± 0.016	0.584LysTyr: 0.584 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.889LeuAla: 13.889 ± 0.13	0.807LeuCys: 0.807 ± 0.029	6.085LeuAsp: 6.085 ± 0.078	4.223LeuGlu: 4.223 ± 0.069	3.613LeuPhe: 3.613 ± 0.065	8.07LeuGly: 8.07 ± 0.076	1.971LeuHis: 1.971 ± 0.04	5.805LeuIle: 5.805 ± 0.07	2.958LeuLys: 2.958 ± 0.061	9.744LeuLeu: 9.744 ± 0.108	2.257LeuMet: 2.257 ± 0.05	2.478LeuAsn: 2.478 ± 0.045	5.661LeuPro: 5.661 ± 0.064	2.56LeuGln: 2.56 ± 0.053	6.917LeuArg: 6.917 ± 0.077	6.235LeuSer: 6.235 ± 0.071	5.597LeuThr: 5.597 ± 0.083	6.759LeuVal: 6.759 ± 0.075	1.228LeuTrp: 1.228 ± 0.032	1.979LeuTyr: 1.979 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	3.56MetAla: 3.56 ± 0.051	0.164MetCys: 0.164 ± 0.012	1.103MetAsp: 1.103 ± 0.03	0.958MetGlu: 0.958 ± 0.026	0.734MetPhe: 0.734 ± 0.027	1.85MetGly: 1.85 ± 0.036	0.445MetHis: 0.445 ± 0.016	1.684MetIle: 1.684 ± 0.035	0.932MetLys: 0.932 ± 0.028	2.892MetLeu: 2.892 ± 0.053	0.783MetMet: 0.783 ± 0.025	0.687MetAsn: 0.687 ± 0.023	1.559MetPro: 1.559 ± 0.039	0.646MetGln: 0.646 ± 0.027	1.873MetArg: 1.873 ± 0.036	1.509MetSer: 1.509 ± 0.036	1.783MetThr: 1.783 ± 0.041	1.649MetVal: 1.649 ± 0.038	0.235MetTrp: 0.235 ± 0.014	0.271MetTyr: 0.271 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.159AsnAla: 3.159 ± 0.06	0.245AsnCys: 0.245 ± 0.016	1.521AsnAsp: 1.521 ± 0.056	1.038AsnGlu: 1.038 ± 0.03	0.965AsnPhe: 0.965 ± 0.029	2.494AsnGly: 2.494 ± 0.074	0.477AsnHis: 0.477 ± 0.019	1.488AsnIle: 1.488 ± 0.039	0.594AsnLys: 0.594 ± 0.024	2.537AsnLeu: 2.537 ± 0.045	0.585AsnMet: 0.585 ± 0.019	0.683AsnAsn: 0.683 ± 0.026	1.735AsnPro: 1.735 ± 0.037	0.692AsnGln: 0.692 ± 0.023	1.84AsnArg: 1.84 ± 0.038	1.269AsnSer: 1.269 ± 0.038	1.088AsnThr: 1.088 ± 0.033	2.021AsnVal: 2.021 ± 0.054	0.403AsnTrp: 0.403 ± 0.018	0.704AsnTyr: 0.704 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.107ProAla: 7.107 ± 0.09	0.319ProCys: 0.319 ± 0.017	3.683ProAsp: 3.683 ± 0.058	3.092ProGlu: 3.092 ± 0.049	1.957ProPhe: 1.957 ± 0.042	4.653ProGly: 4.653 ± 0.067	1.09ProHis: 1.09 ± 0.029	2.963ProIle: 2.963 ± 0.05	1.615ProLys: 1.615 ± 0.047	4.818ProLeu: 4.818 ± 0.071	1.247ProMet: 1.247 ± 0.03	1.312ProAsn: 1.312 ± 0.036	2.794ProPro: 2.794 ± 0.059	1.564ProGln: 1.564 ± 0.036	2.953ProArg: 2.953 ± 0.053	2.818ProSer: 2.818 ± 0.047	2.675ProThr: 2.675 ± 0.052	4.053ProVal: 4.053 ± 0.056	0.669ProTrp: 0.669 ± 0.025	1.13ProTyr: 1.13 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.181GlnAla: 4.181 ± 0.066	0.181GlnCys: 0.181 ± 0.012	1.36GlnAsp: 1.36 ± 0.034	1.101GlnGlu: 1.101 ± 0.033	1.033GlnPhe: 1.033 ± 0.029	2.425GlnGly: 2.425 ± 0.042	0.613GlnHis: 0.613 ± 0.023	1.952GlnIle: 1.952 ± 0.044	0.915GlnLys: 0.915 ± 0.03	2.989GlnLeu: 2.989 ± 0.047	0.818GlnMet: 0.818 ± 0.028	0.79GlnAsn: 0.79 ± 0.03	1.77GlnPro: 1.77 ± 0.04	1.095GlnGln: 1.095 ± 0.03	2.28GlnArg: 2.28 ± 0.049	1.712GlnSer: 1.712 ± 0.04	1.657GlnThr: 1.657 ± 0.032	2.103GlnVal: 2.103 ± 0.042	0.354GlnTrp: 0.354 ± 0.015	0.616GlnTyr: 0.616 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.662ArgAla: 8.662 ± 0.111	0.536ArgCys: 0.536 ± 0.019	4.244ArgAsp: 4.244 ± 0.064	3.591ArgGlu: 3.591 ± 0.058	3.024ArgPhe: 3.024 ± 0.052	5.03ArgGly: 5.03 ± 0.069	1.781ArgHis: 1.781 ± 0.039	4.556ArgIle: 4.556 ± 0.069	2.152ArgLys: 2.152 ± 0.042	8.154ArgLeu: 8.154 ± 0.104	2.034ArgMet: 2.034 ± 0.041	1.888ArgAsn: 1.888 ± 0.036	3.806ArgPro: 3.806 ± 0.057	2.405ArgGln: 2.405 ± 0.043	5.862ArgArg: 5.862 ± 0.081	3.966ArgSer: 3.966 ± 0.058	3.65ArgThr: 3.65 ± 0.065	4.513ArgVal: 4.513 ± 0.057	1.097ArgTrp: 1.097 ± 0.028	1.829ArgTyr: 1.829 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	7.084SerAla: 7.084 ± 0.126	0.443SerCys: 0.443 ± 0.019	3.155SerAsp: 3.155 ± 0.054	2.474SerGlu: 2.474 ± 0.051	2.198SerPhe: 2.198 ± 0.047	5.706SerGly: 5.706 ± 0.095	1.125SerHis: 1.125 ± 0.027	3.069SerIle: 3.069 ± 0.054	1.426SerLys: 1.426 ± 0.039	5.477SerLeu: 5.477 ± 0.085	1.281SerMet: 1.281 ± 0.032	1.388SerAsn: 1.388 ± 0.041	2.952SerPro: 2.952 ± 0.053	1.546SerGln: 1.546 ± 0.037	3.579SerArg: 3.579 ± 0.054	3.149SerSer: 3.149 ± 0.074	2.866SerThr: 2.866 ± 0.054	3.836SerVal: 3.836 ± 0.06	0.753SerTrp: 0.753 ± 0.024	1.482SerTyr: 1.482 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	6.712ThrAla: 6.712 ± 0.105	0.372ThrCys: 0.372 ± 0.019	2.925ThrAsp: 2.925 ± 0.05	2.232ThrGlu: 2.232 ± 0.04	1.863ThrPhe: 1.863 ± 0.037	5.338ThrGly: 5.338 ± 0.12	1.007ThrHis: 1.007 ± 0.027	3.519ThrIle: 3.519 ± 0.064	1.35ThrLys: 1.35 ± 0.033	5.553ThrLeu: 5.553 ± 0.081	1.334ThrMet: 1.334 ± 0.033	1.42ThrAsn: 1.42 ± 0.055	3.178ThrPro: 3.178 ± 0.048	1.416ThrGln: 1.416 ± 0.033	3.381ThrArg: 3.381 ± 0.057	2.968ThrSer: 2.968 ± 0.078	3.085ThrThr: 3.085 ± 0.072	4.044ThrVal: 4.044 ± 0.063	0.547ThrTrp: 0.547 ± 0.021	1.166ThrTyr: 1.166 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	9.705ValAla: 9.705 ± 0.094	0.52ValCys: 0.52 ± 0.022	4.471ValAsp: 4.471 ± 0.066	4.014ValGlu: 4.014 ± 0.067	2.004ValPhe: 2.004 ± 0.042	5.56ValGly: 5.56 ± 0.065	1.382ValHis: 1.382 ± 0.033	3.954ValIle: 3.954 ± 0.055	1.948ValLys: 1.948 ± 0.041	6.301ValLeu: 6.301 ± 0.08	1.671ValMet: 1.671 ± 0.038	1.879ValAsn: 1.879 ± 0.04	3.82ValPro: 3.82 ± 0.056	1.994ValGln: 1.994 ± 0.04	5.106ValArg: 5.106 ± 0.068	4.254ValSer: 4.254 ± 0.077	4.274ValThr: 4.274 ± 0.068	5.16ValVal: 5.16 ± 0.075	0.732ValTrp: 0.732 ± 0.024	1.321ValTyr: 1.321 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.324TrpAla: 1.324 ± 0.038	0.129TrpCys: 0.129 ± 0.01	0.66TrpAsp: 0.66 ± 0.021	0.505TrpGlu: 0.505 ± 0.021	0.509TrpPhe: 0.509 ± 0.023	0.847TrpGly: 0.847 ± 0.024	0.363TrpHis: 0.363 ± 0.018	0.711TrpIle: 0.711 ± 0.023	0.427TrpLys: 0.427 ± 0.02	1.64TrpLeu: 1.64 ± 0.043	0.35TrpMet: 0.35 ± 0.018	0.422TrpAsn: 0.422 ± 0.018	0.723TrpPro: 0.723 ± 0.022	0.59TrpGln: 0.59 ± 0.023	1.25TrpArg: 1.25 ± 0.029	0.857TrpSer: 0.857 ± 0.025	0.765TrpThr: 0.765 ± 0.024	0.757TrpVal: 0.757 ± 0.027	0.232TrpTrp: 0.232 ± 0.015	0.275TrpTyr: 0.275 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.675TyrAla: 2.675 ± 0.042	0.216TyrCys: 0.216 ± 0.013	1.675TyrAsp: 1.675 ± 0.049	1.066TyrGlu: 1.066 ± 0.029	0.847TyrPhe: 0.847 ± 0.025	2.133TyrGly: 2.133 ± 0.044	0.498TyrHis: 0.498 ± 0.019	0.909TyrIle: 0.909 ± 0.026	0.581TyrLys: 0.581 ± 0.024	2.077TyrLeu: 2.077 ± 0.046	0.433TyrMet: 0.433 ± 0.019	0.612TyrAsn: 0.612 ± 0.025	0.991TyrPro: 0.991 ± 0.031	0.692TyrGln: 0.692 ± 0.024	1.902TyrArg: 1.902 ± 0.043	1.238TyrSer: 1.238 ± 0.039	1.02TyrThr: 1.02 ± 0.037	1.591TyrVal: 1.591 ± 0.042	0.333TyrTrp: 0.333 ± 0.016	0.586TyrTyr: 0.586 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4037 proteins (1304086 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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