Cotton leaf curl Alabad virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9W8K2|C9W8K2_9GEMI Replication-associated protein OS=Cotton leaf curl Alabad virus OX=222456 GN=AC1 PE=3 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84SSSHH6 pKa = 6.67LKK8 pKa = 10.41DD9 pKa = 3.35PCTHH13 pKa = 5.67VPIKK17 pKa = 9.65VQHH20 pKa = 6.42RR21 pKa = 11.84EE22 pKa = 3.38AKK24 pKa = 9.26RR25 pKa = 11.84RR26 pKa = 11.84IRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84VDD33 pKa = 3.86LEE35 pKa = 4.55CGCSYY40 pKa = 10.87YY41 pKa = 11.12LSINCHH47 pKa = 3.96NHH49 pKa = 5.13GFTHH53 pKa = 7.48RR54 pKa = 11.84GTHH57 pKa = 5.76HH58 pKa = 6.75CSSSRR63 pKa = 11.84EE64 pKa = 3.59WRR66 pKa = 11.84IYY68 pKa = 10.76LGGSKK73 pKa = 10.35SPIFQDD79 pKa = 3.24HH80 pKa = 6.0QPRR83 pKa = 11.84QPSIHH88 pKa = 7.24DD89 pKa = 3.67EE90 pKa = 3.82YY91 pKa = 11.2GYY93 pKa = 8.04THH95 pKa = 7.12DD96 pKa = 4.34QDD98 pKa = 4.41PVQLQHH104 pKa = 6.48SEE106 pKa = 4.17SSGTAQVFSNLPNLDD121 pKa = 4.54DD122 pKa = 4.57LTPSDD127 pKa = 3.25WSFLKK132 pKa = 10.89GIQNPSPQISEE143 pKa = 3.81QSRR146 pKa = 11.84CNFNN150 pKa = 3.23

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9W8K0|C9W8K0_9GEMI Replication enhancer OS=Cotton leaf curl Alabad virus OX=222456 GN=AC3 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 10.21RR4 pKa = 11.84AADD7 pKa = 3.55IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.01SPYY27 pKa = 9.88ACRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 9.91QQAWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 8.9PEE54 pKa = 4.27CTGCTEE60 pKa = 3.55VRR62 pKa = 11.84MFQGDD67 pKa = 3.2EE68 pKa = 4.1RR69 pKa = 11.84VPVRR73 pKa = 11.84YY74 pKa = 9.69SRR76 pKa = 11.84LNLDD80 pKa = 3.27TMSFILVRR88 pKa = 11.84YY89 pKa = 9.11CVFRR93 pKa = 11.84MLRR96 pKa = 11.84VEE98 pKa = 4.39SVCTHH103 pKa = 6.92RR104 pKa = 11.84IGKK107 pKa = 8.5RR108 pKa = 11.84FCVKK112 pKa = 9.96SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.57NSHH172 pKa = 6.59RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 9.17QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1118

96

362

186.3

21.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.204 ± 0.795

3.309 ± 0.69

3.846 ± 0.519

4.83 ± 0.746

4.204 ± 0.469

4.114 ± 0.321

3.667 ± 0.608

5.367 ± 0.749

5.456 ± 0.466

7.871 ± 0.991

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.414

5.188 ± 0.736

6.261 ± 0.786

4.83 ± 0.412

7.961 ± 0.899

8.318 ± 1.428

5.903 ± 0.492

6.351 ± 1.263

1.252 ± 0.115

4.293 ± 0.683
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski